• 한국염색가공학회지
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• 제21권5호
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• pp.1-9
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• 2009

The feasibility of earthworm protease as a new cosmetic material for human hair care was investigated. The effectiveness of the earthworm protease treatment was assessed by thickness change of hairs, optical microscope examination, aminoacid analysis, surface morphology, angular resolution analysis through methylene blue staining method and tensile strength change. The protease treated hair became thinner and the soil on the surface removed in experimental groups unlike control group. Tensile strength decreased in experimental group in which the enzymes may decompose polypeptide bonds.

• 한국식품저장유통학회지
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• 제8권2호
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• pp.187-192
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• 2001

Amino acid composition of proteins in Italian millet, Common millet and sorghum were invstigated by HCI hydrolysis method. The optimum condition was obtained by hydrolysis at 110$\^{C}$ for 24hr. As major amino acids from protein hydrolyzate, the content of tyosine, arginine and phebylalanine were 7.06%, 6.79% and 6.44%, respectively. The content of glutamic acid in Common millet, Italian millet and Sorghum were 5.73%, 5.64% and 5.46%, respectively. Glycine content was about 2.93% in three samples. Contents of crude protein and pure protein in Italian millet, Common millet and sorghum were determined by micro-kjeldahl method. Crude protein contents were slightly higher than that of pure protein. Protein content of sorghum was higher than those of Italian millet and Common millet. For SDS-PAGE analysis, Italian millet showed more soluble proteins including 50kDa, 30kDa and smaller proteins than other cereals. In particular, Common millet and Sorghum only solubilized proteins less than 15kDa.

• International Journal of Industrial Entomology and Biomaterials
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• 제15권2호
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• pp.123-130
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• 2007

The Hsp 20.8 and Hsp 90 cDNA sequence retrieved from NCBI database and consists of 764 bp and 2582 bp lengths respectively. The corresponding cDNA homologus sequences were BLAST searched in Bombyx mori genomic DNA database and two genomic contigs viz., BAAB01120347 and AADK01011786 showed maximum homology. In B. mori Hsp 20.8 and Hsp 90 is encoded by single gene without intron. Specific primers were used to amplify the Hsp 20.8 gene and Hsp 90 variable region from genomic DNA by using the PCR. Obtained products were 216 bp in Hsp 20.8 and 437 bp in Hsp 90. There was no variation found in the six silkworm races PCR products size of contrasting response to thermal tolerance. The comparison of the sequenced nucleotide variations through multiple sequence alignment analysis of Hsp 90 variable region products of three races not showed any differences respect to their thermotolerance and formed the clusters among the voltinism. The comparison of aminoacid sequences of B. mori Hsps with dipteran and other insect taxa revealed high percentage of identity growing with phylogenetic relatedness of species. The conserved domains of B. mori Hsps predicted, in which the Hsp 20.8 possesses ${\alpha}-crystallin$ domain and Hsp 90 holds HATPase and Hsp 90 domains.

• The Plant Pathology Journal
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• 제21권2호
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• pp.149-157
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• 2005

A novel rice (Oryza sativa L.) gene, homologous to Arabidopsis pathogenesis-related NDR1 gene, was cloned from cDNA library prepared from 30 min Magnaporthe grisea -treated rice seedling leaves, and named as OsNDR1. OsNDR1 encoded a 220-aminoacid polypeptide and was highly similar to the Arabidopsis AtNDR1 protein. OsNDR1 is a plasma membrane (PM)-localized protein, and presumes through sequence analysis and protein localization experiment. Overexpression of OsNDR1 promotes the expression of PBZ1 that is essential for the activation of defense/stressrelated gene. The OsNDR1 promoter did not respond significantly to treatments with either SA, PBZ, or ETP. Exogenously applied BTH induces the same set of SAR genes as biological induction, providing further evidence for BTH as a signal. Presumably, BTH is bound by a receptor and the binding triggers a signal transduction cascade that has an ultimate effect on transcription factors that regulate SAR gene expression. Thus OsNDR1 may act as a transducer of pathogen signals and/or interact with the pathogen and is indeed another important step in clarifying the component participating in the defense response pathways in rice.

• 한국식품영양과학회지
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• 제33권5호
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• pp.778-784
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• 2004

인삼을 재배한 후 널리 이용되지 못하고 있는 잎, 줄기 등 인삼 부산물의 활용방안을 모색하고자 연구를 수행하였다. 이에 따라 인삼잎과 줄기를 혼합하여 추출한 추출물을 대상으로 일반 영양성분 함량, 무기질 함량, 아미노산 및 지방산 조성을 분석하고 세포독성에 미치는 영향을 조사하여 다음과 같은 결과를 얻었다. 인삼잎, 줄기 혼합 추출물의 수분함량은 96.61%, 탄수화물 2.51%, 조회분 0.53%, 조지방 0.20% 및 조단백질이 0.15%이었다. 무기질 중 칼륨함량이 102.56 mg/100 g으로 가장 높았고 그 외 칼슘, 마그네슘, 나트륨, 인 및 철분, 망간, 아연, 구리도 함유되어 있었다. Glutamic acid와 aspartic acid 등 산성아미노산 함량이 높게 나타났고 필수아미노산이 모두 함유되어 있었다. 또한 불포화지방산 함량은 56.52%이었고 palmitic acid 함량이 39.99%로 가장 높았고, linoleic acid 14.96%, docosatetranoic acid 13.31%, linolenic acid 12.19%의 순이었다. 인삼의 총 ginenoside 함량은 0.82 mg/mL이 었고 PD/PT 함유 비율은 0.68이었다. 정상적인 간과 신장 세포의 생존율에는 negative effect를 나타내지 않았고 미토콘드리아와 라이소좀 수준에서 세포독성을 나타내지 않았다. 인삼잎, 줄기 혼합 추출물의 약리효능을 나타내는 gensenoside의 함량이 0.82 mg/mL로서 비교적 소량 함유되어 있으나 인삼 부산물의 활용차원에서 동물의 사료나 기능성 식품 재료로의 사용은 가능할 것으로 생각된다. 향후 동물실험 및 임상실험을 이용한 기능성 평가를 통해 확실하고 구체적인 활용방안의 모색이 요구되어진다.