• Korean Journal of Soil Science and Fertilizer
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• v.10 no.1
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• pp.29-37
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• 1977

The mineralization of soil nitrogen, amino acid composition of acid hydrolizable organic nitrogen of four Korean soils were investigated in comparison with four Japanese (Hokuriku district) soils which is similar in nitrogen content but different in characteristics of clay minerals. The mineralization rate and pattern were quite different between Korean and Japanese soils; Korean soils were low in amount of mineralized nitrogen but porduced much ammonium nitrogen during the later stage of incubation. In Korean soils the ratio of acid hydrolizable nitrogen to total; especially ${\alpha}$-amino nitrogen and hydrolizable ammonium nitrogen were low while hexosamine content was considerablly high (greater than 10%) In all soils the amount of mineralized nitrogen showed significant positive correlation with ammonium nitrogen and ${\alpha}$-amino nitrogen in acid hydrolizate. The amino acid composition of acid hydrolizate of paddy soils showed higher in basic amino acids and lower in acidic amino acids than those of up land soils (humic volcanic ash soil) from both countries. Alanine content was low in Korean soils. Proline showed increasing trend with nitrogen content but aspartic acid decreasing.

• Journal of the East Asian Society of Dietary Life
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• v.23 no.1
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• pp.92-97
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• 2013

This study was conducted to understand the quality characteristics of salt fermented anchovies with heat treatment by measuring their chemical compositions. The heat-treated and non-heat treated salt fermented anchovies contained, respectively, 63.21 and 66.51% of moisture, 2.24 and 2.12% of total nitrogen (TN), and 1,537 and 1,520 mg/100 g of amino nitrogen (AN). In addition, heat-treated and non-heat-treated salt fermented anchovies contained 127 and 134 mg/100 g of volatile basic nitrogen (VBN), respectively. Moreover, measured the microbial level of heat-treated and non-heat-treated salt fermented anchovies was $2.58{\times}10^4$ and $3.61{\times}10^2$ CFU/mL, respectively. Also, the heat-treated and non-heat-treated salt fermented anchovies 3.65 and 0.30 units of protease activities, respectively. The total free amino acid contents in heat-treated and non-heat-treated salt fermented anchovies was 4,964 and 6,638 mg/100 g, respectively. The major free amino acid were glutamic acid, leucine, lysine, alanine, valine, isoleucine. Our results provide the characteristics of salt fermented anchovies and encourage their application for the food industry and cooking.

• Journal of Plant Biology
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• v.16 no.1_2
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• pp.30-38
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• 1973

To study the response to plant growth by the environmental factors, the effects of application of nitrogen on changes in the yield, crude protein, amino acids, chlorophyll, carotene, total phosphorus, acid-soluble phosphorus, phospholipids, RNA, and DNA were investigated with westerworlds 9Lolium sublatum) and perennial rye-grasses (Lolium perenne). The amounts of dry weight, crude protein, amino acids, chlorophyll, carotene, total phosphorus, acid-soluble phosphorus, phospholipids, RNA and DNA of both rye-grasses increased with adequately increasing nitrogen, and reached a maximum with an adequate application of nitrogen. The relationships between yields and crude protein contents, crude protein and RNA contents, and yields and RNA contents of westerworlds and perennial rye-grasses were found to be positively correlated, respectively. Therefore, in general, the response to plant growth by the environmental factors such as nitrogen nutrient may be summarized as follows: Environmental factors\longrightarrowDNA\longrightarrowRNA\longrightarrowProtein\longrightarrowPlant growth

• The Korean Journal of Ecology
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• v.22 no.3
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• pp.109-117
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• 1999

Thirty two species of coastal plants (mainly halophytes) were investigated for their free amino acids and the total and water-soluble nitrogen contents in leaves. All plants except some species (e.g. Scutellaria and Linaria) contained proline, but only Ageratum. Tetragonia and Raphanus in rather high amounts, that it can be thought to serve as a cytoplasmic osmolyte. In some plant species (Euphorbia. Glehnia. Peucedanum. Raphanus and monocotyledonous Carex and Zoysia), however, hydroxyproline. (OH-Pro) rather than proline were accumulated to a considerable extent. The concentrations of total free amino acids were low in Aster tripolium, Linaria. Lysimachia. Plantago. Rumex, Vitex and especially in the members of the Chenopodiaceae and Crassulaceae. Marked differences also occurred in the nitrogen levels. Aizoaceae, Chenopodiaceae, Convolvulaceae, Cruciferae, Euphorbiaceae and Leguminosae usually showed high values of total and soluble amino nitrogen, while the opposite was true for most of the Cyperaceae, Gramineae, Lamiaceae, Plantaginaceae, Scophuriaceae, and Verbenaceae. The free amino acids in the investigated plant species contributed very little to the nitrogen content, but in plants of Euphorbia, Messerschmidia and Orostachys. their amino acid-N made up for 25∼30% of the total nitrogen. In conclusion, only a few cases did proline known as compatible solute constitute a significant proportion of the free amino acid pool in coastal plants.

• ALGAE
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• v.29 no.3
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• pp.227-235
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• 2014

The red seaweed, Gracilaria tikvahiae McLachlan, was cultivated in open water farms in urbanized estuaries of Long Island Sound (26-30 psu of salinity) and New York City (20-25 psu), USA in 2011. Plants were harvested monthly from summer (August, $24^{\circ}C$) to fall (November, $13^{\circ}C$) and analyzed for total nitrogen, protein, and amino acid content. On a dry matter (DM) basis, nitrogen and protein significantly increased over the harvest period until October and then plateaued. Nitrogen increased from $22{\pm}1g\;kg^{-1}$ DM in August to $39{\pm}3g\;kg^{-1}$ DM in October (p < 0.001). Protein increased from $107{\pm}13g\;kg^{-1}$ DM in August to $196{\pm}5g\;kg^{-1}$ DM in November (p < 0.001). With two exceptions, amino acid concentrations expressed on a crude protein (CP) basis were similar over the harvest period. Essential amino acids accounted for $48{\pm}1%$ of all amino acids present with lysine and methionine averaging $56{\pm}2g\;kg^{-1}$ CP and $18{\pm}1g\;kg^{-1}$ CP, respectively. Histidine was underrepresented among essential amino acids and averaged $13{\pm}1g\;kg^{-1}$ CP. Taurine ranged from 2.1 to $3.2g\;kg^{-1}$ DM. With its moderate levels of lysine, methionine and taurine, ocean farmed G. tikvahiae has the potential of overcoming many nutrient deficiencies currently associated with terrestrial plant ingredients in alternative feeds for fish and shrimp.

• Korean Journal of Fisheries and Aquatic Sciences
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• v.15 no.1
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• pp.52-58
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• 1982

The effects of casein diet supplemented with excessive levels of three basic amino acidslysine, histidine and arginine - were studied in male, weanling, I. C. R. strain mice. The relationship between weight gain and concentration of blood urea nitrogen was also discussed with the follows results : 1. The groups supplemented with basic amino acids were demonstrated depressed growth and food intake than the control ($10\%$ casein diet) group, $5\%$ L-histidine HCl supplemented group being most depressed. 2. The concentration of blood urea nitrogen in the basic amino acid supplemented groups were higher than the control group. The value of L-arginine supplemented group was highest. 3. The concentration of blood urea nitrogen was related to the amounts of nitrogen contained in the diet not related to the growth gain.

• Asian-Australasian Journal of Animal Sciences
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• v.15 no.10
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• pp.1507-1516
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• 2002

Meat and bone meal is a valuable protein and mineral source in diets of production animals and contributes to the protein, energy and mineral component of diets. The aim of the present study was to more accurately characterise the apparent ileal amino acid digestibility of meat and bone meals produced in New Zealand and evaluate routine in vitro assays used in practise to measure meat and bone meal quality. A total of 94 commercial meat and bone meals from 25 New Zealand rendering plants over a two and a half year period were analysed for proximates, gross energy, gross amino acid content (incl. hydroxyproline, hydroxylysine and lanthionine), apparent ileal amino acid digestibility, pepsin nitrogen digestibility, protein solubility and bone content. The mean crude protein content of the 94 meat and bone meal samples was 56.8% with a range of >35% units and a coefficient of variation of 9.8%. The mean crude fat and ash content were 10.0 and 28.4% respectively. These latter components showed a large range (16 and 43%, respectively) with coefficients of variation above 22%. Amino acid digestibility between samples was highly variable with lysine and sulphur amino acids digestibility ranging between 45.8-89.0 and 38.2-85.5%, respectively. Pearson correlation coefficients are presented between crude protein content and individual gross amino acids, crude protein content and individual digestible amino acid content, and pepsin N digestibility and individual digestible amino acid content. There was a significant relationship between the digestible amino acid nitrogen content and the crude protein content while pepsin nitrogen digestibility was not correlated to ileal amino acid nitrogen digestibility (r=-0.06). Meat meals with a high protein content had relatively low hydroxyproline and hydroxylysine levels something that was attributed to the levels of collagen from bone. The data indicated that lanthionine (formed upon heat treatment of cysteine with a hydroprotein) is not a good indicator of the heat treatment employed to meat and bone meals. Step-wise multiple regression equations to predict the apparent digestible content of amino acids from rapid in vitro assays are presented. The most selected variables included ash and crude fat content. In general the equations derived for the essential amino acids had a higher degrees of fit (R2) compared to the non-essential amino acids. The R2 for the essential amino acids ranged from 0.43 for histidine and 0.68 for leucine. These equations provide a means of more rapidly estimating the apparent ileal digestible amino acid content (protein quality) of meat and bone meal using standard analyses.

• Asian-Australasian Journal of Animal Sciences
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• v.21 no.2
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• pp.232-236
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• 2008

This experiment was performed to investigate the effects of two energy levels and four lysine:digestible energy (DE) ratios on the apparent digestibility of nutrients in finishing pigs. The experiment was conducted using a $2{\times}4$ randomized complete block (RCB) design with three replicates. Twenty-four cross-bred finishing barrows ((Landrace${\times}$Yorkshire)${\times}$Duroc) with an average body weight of $64.2{\pm}0.69kg$ were assigned to one of eight treatments. Each barrow was placed in an individual metabolism crate and dietary treatment and water was provided ad libitum. Diets were designed to contain lysine:ME ratios of 1.5, 1.8, 2.1 and 2.4 g/Mcal at 3.35 and 3.6 Mcal/kg of diet in a $4{\times}2$ factorial arrangement. Dry matter (DM), ash, Ca and P digestibility were not affected by energy density or lysine:DE ratios. Crude fat digestibility increased as the energy density increased from 3.35 to 3.6 Mcal of DE/kg. Increasing the lysine:DE ratio also increased crude protein digestibility. There were no interactions between energy density and lysine:DE ratio in terms of nutrient digestibility. Nitrogen excretion via feces was not affected by energy density and lysine:DE ratio, while nitrogen excretion via urine was significantly affected by energy density and lysine:DE ratio. The apparent digestibility of all amino acids except for isoluecine, arginine and aspartic acid as well as average values of essential amino (EAA), non-essential amino acids (NEAA) and total amino acid digestibility (p>0.05) were not affected by energy density. The apparent digestibility of all amino acids except for leucine, proline, alanine and tyrosine, NEAA and total amino acid digestibility were significantly affected by lysine: DE ratio (p<0.05). Interactive effects of energy and lysine:DE ratio also significantly affected amino acid digestibility except for isoleucine, alanine, cystine, leucine, phenylalanine, glutamine and proline (p<0.05). In conclusion, these results suggest that maintaining the appropriate lysine:DE ratio becomes more important as the energy density of the diet increases. Consequently, increasing the lysine:DE ratio can result in increased crude protein digestibility and urinary nitrogen excretion, although apparent protein digestibility and nitrogen excretion were not affected by energy density Furthermore, increasing the lysine:DE ratio also increased the apparent digestibility of essential amino acids, except for leucine, regardless of energy density. The optimum lysine:DE ratio for maximum essential amino acid digestibility of the $64.2{\pm}0.69kg$ pig is approximately 2.4 g of lysine/Mcal of DE.

• Korean Journal of Food Science and Technology
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• v.6 no.1
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• pp.12-16
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• 1974

The amino acid pattern of embryo and endosperm of brown rice different in specific gravity was investigated using Jinheung (local leading temperate variety) and IR667-Suweon 213 (high-yielding newly bred tropical variety). 1. Embryo of IR667 (higher protein rice) showed lower protein, and lower lysine or essential amino acid per protein than that of Jinheung (lower protein rice). 2. In both embryo and endosperm nitrogen recovery as amino acids was highest in middle class of specific gravity and lowest in low class indicating that abundancy of non-protein nitrogen in low class and decomposition of amino acids by starch in high specific gravity class. 3. In both embryo and endosperm IR667 showed abundancy in order of glutamic acid, aspartic acid while Jinheung showed glutamic acid, arginine, suggesting varietal difference in nitrogen metabolism. 4. In both IR667 and Jinheung least amino acid was histidine and next leucine in embryo but histidine and next threonine in endosperm, suggesting organ difference in nitrogen metabolism.

• Journal of Microbiology and Biotechnology
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• v.16 no.7
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• pp.999-1009
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• 2006

Physiological, biochemical and genetic studies of Corynebacterium glutamicum, a workhorse of white biotechnology used for amino acid production, led to a waste knowledge mainly about amino acid biosynthetic pathways and the central carbon metabolism of this bacterium. Spurred by the availability of the genome sequence and of genome-based experimental methods such as DNA microarray analysis, research on genetic regulation came into focus. Recent progress on mechanisms of genetic regulation of the carbon, nitrogen, sulfur and phosphorus metabolism in C. glutamicum will be discussed.