Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Genetic Regulation of Corynebacterium glutamicum Metabolism

  • Wendisch Volker F. (Institute of Molecular Microbiology and Biotechnology, Westfalian Wilhelms University Muenster)
  • Published : 2006.07.01
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Abstract

Physiological, biochemical and genetic studies of Corynebacterium glutamicum, a workhorse of white biotechnology used for amino acid production, led to a waste knowledge mainly about amino acid biosynthetic pathways and the central carbon metabolism of this bacterium. Spurred by the availability of the genome sequence and of genome-based experimental methods such as DNA microarray analysis, research on genetic regulation came into focus. Recent progress on mechanisms of genetic regulation of the carbon, nitrogen, sulfur and phosphorus metabolism in C. glutamicum will be discussed.

Keywords

References

  1. Barreiro, C., E. Gonzalez-Lavado, S. Brand, A. Tauch, and J. F. Martin. 2005. Heat shock proteome analysis of wild-type Corynebacterium glutamicum ATCC 13032 and a spontaneous mutant lacking GroEL 1, a dispensable chaperone. J. Bacteriol. 187: 884-889 https://doi.org/10.1128/JB.187.3.884-889.2005
  2. Baumbach, J., K. Brinkrolf, L. F. Czaja, S. Rahmann, and A. Tauch. 2006. CoryneRegNet: An ontology-based data warehouse of corynebacterial transcription factors and regulatory networks. BMC Genomics 7: 24 https://doi.org/10.1186/1471-2164-7-24
  3. Beckers, G., A. K. Bendt, R. Kramer, and A. Burkovski. 2004. Molecular identification of the urea uptake system and transcriptional analysis of urea transporter- and urease- encoding genes in Corynebacterium glutamicum. J. Bacteriol. 186: 7645-7652 https://doi.org/10.1128/JB.186.22.7645-7652.2004
  4. Beckers, G, J. Strosser, U. Hildebrandt, J. Kalinowski, M. Farwick, R. Kramer, and A. Burkovski. 2005. Regulation of AmtR-controlled gene expression in Corynebacterium glutamicum: Mechanism and characterization of the AmtR regulon. Mol. Microbiol. 58: 580-595 https://doi.org/10.1111/j.1365-2958.2005.04855.x
  5. Bellmann, A., M. Vrljic, M. Patek, H. Sahm, R. Kramer, and L. Eggeling. 2001. Expression control and specificity of the basic amino acid exporter LysE of Corynebacterium glutamicum. Microbiology 147: 1765-1774 https://doi.org/10.1099/00221287-147-7-1765
  6. Bendt, A. K., G. Beckers, M. Silberbach, A. Wittmann, and A. Burkovski. 2004. Utilization of creatinine as an alternative nitrogen source in Corynebacterium glutamicum. Arch. Microbiol. 181: 443-50 https://doi.org/10.1007/s00203-004-0679-z
  7. Bendt, A. K., A. Burkovski, S. Schaffer, M. Bott, M. Farwick, and T. Hermann. 2003. Towards a phosphoproteome map of Corynebacterium glutamicum. Proteomics 3: 1637-1646 https://doi.org/10.1002/pmic.200300494
  8. Brune, I., K. Brinkrolf, J. Kalinowski, A. Puehler, and A. Tauch. 2005. The individual and common repertoire of DNA-binding transcriptional regulators of Corynebacterium glutamicum, Corynebacterium efficiens, Corynebacterium diphtheriae, and Corynebacterium jeikeium deduced from the complete genome sequences. BMC Genomics 6: 86 https://doi.org/10.1186/1471-2164-6-86
  9. Brune, I., H. Werner, A. T. Huser, J. Kalinowski, A. Puhler, and A. Tauch. 2006. The DtxR protein acting as dual transcriptional regulator directs a global regulatory network involved in iron metabolism of Corynebacterium glutamicum. BMC Genomics 7: 21 https://doi.org/10.1186/1471-2164-7-21
  10. Burkovski, A. 2005. Nitrogen metabolism and its regulation, pp. 333-350. In Eggeling. L. and Bott, M. (eds.), Handbook of Corynebacterium glutamicum. CRC Press, Boca Raton, U.S.A
  11. Chami, M., N. Bayan, J. Dedieu, G. Leblon, E. Shechter, and T. Gulik-Krzywicki. 1995. Organization of the outer layers of the cell envelope of Corynebacterium glutamicum: A combined freeze-etch electron microscopy and biochemical study. BioI. Cell. 83: 219-229 https://doi.org/10.1016/0248-4900(96)81311-6
  12. Chami, M., N. Bayan, J. L. Peyret, T. Gulik-Krzywicki, G. Leblon, and E. Shechter. 1997. The S-layer protein of Corynebacterium glutamicum is anchored to the cell wall by its C-terminal hydrophobic domain. Mol. Microbiol. 23: 483-492 https://doi.org/10.1046/j.1365-2958.1997.d01-1868.x
  13. Claes, W. A., A. Puhler, and J. Kalinowski. 2002. Identification of two prpDBC gene clusters in Corynebacterium glutamicum and their involvement in propionate degradation via the 2methylcitrate cycle. J. Bacteriol. 184: 2728-2739 https://doi.org/10.1128/JB.184.10.2728-2739.2002
  14. Cooper, A., A. McAlpine, and P. G. Stockley. 1994. Calorimetric studies of the energetics of protein-DNA interactions in the E. coli methionine repressor (MetJ) system. FEBS Lett. 348: 41-45 https://doi.org/10.1016/0014-5793(94)00579-6
  15. Cramer, A., R. Gerstrneir, S. Schaffer, M. Bott, and B. J. Eikmanns. 2006. Identification of Ram A, a novel LuxR-type transcriptional regulator of genes involved in acetate metabolism of Corynebacterium glutamicum. J. Bacteriol. 188: 2554-2567 https://doi.org/10.1128/JB.188.7.2554-2567.2006
  16. Dominguez, H., M. Cocaign-Bousquet, and N. D. Lindley. 1997. Simultaneous consumption of glucose and fructose from sugar mixtures during batch growth of Corynebacterium glutamicum. Appl. Microbiol. Biotechnol. 47: 600-603 https://doi.org/10.1007/s002530050980
  17. Eggeling, L. and M. Bott. 2005. Handbook of Corynebacterium glutamicum. (Eggeling, L. and Bott, M., eds.), CRC Press, Boca Raton, U.S.A
  18. Eikmanns, B. 2005. Central metabolism: Tricarboxylic acid cycle and anaplerotic reactions, pp. 241-276. In Eggeling, L. and Bott, M. (eds.), Handbook on Corynebacterium glutamicum. CRC Press, Boca Raton, U.S.A
  19. Engels, S., C. Ludwig, J. E. Schweitzer, C. Mack, M. Bott, and S. Schaffer. 2005. The transcriptional activator ClgR controls transcription of genes involved in proteolysis and DNA repair in Corynebacterium glutamicum. Mol. Microbiol. 57: 576-591 https://doi.org/10.1111/j.1365-2958.2005.04710.x
  20. Engels, S., J. E. Schweitzer, C. Ludwig, M. Bott, and S. Schaffer. 2004. clpC and clpP1P2 gene expression in Corynebacterium glutamicum is controlled by a regulatory network involving the transcriptional regulators ClgR and HspR as well as the ECF sigma factor sigmaH. Mol. Microbiol. 52: 285-302 https://doi.org/10.1111/j.1365-2958.2003.03979.x
  21. Georgi, T., D. Rittmann, and V. F. Wendisch. 2005. Lysine and glutamate production by Corynebacterium glutamicum on glucose, fructose and sucrose: Roles of malic enzyme and fructose-1,6-bisphosphatase. Metab. Eng. 7: 291-301 https://doi.org/10.1016/j.ymben.2005.05.001
  22. Gerstmeir, R., A. Cramer, P. Dangel, S. Schaffer, and B. J. Eikmanns. 2004. RamB, a novel transcriptional regulator of genes involved in acetate metabolism of Corynebacterium glutamicum. J. Bacteriol. 186: 2798-2809 https://doi.org/10.1128/JB.186.9.2798-2809.2004
  23. Gerstrneir, R., V. F. Wendisch, S. Schnicke, H. Ruan, M. Farwick, D. Reinscheid, and B. J. Eikmanns. 2003. Acetate metabolism and its regulation in Corynebacterium glutamicum. J. Biotechnol. 104: 99-122 https://doi.org/10.1016/S0168-1656(03)00167-6
  24. Grossmann, K., K. Herbster, and M. Mack. 2000. Rapid cloning of metK encoding methionine adenosyltransferase from Corynebacterium glutamicum by screening a genomic library on a high density colony-array. FEMS Microbiol. Lett. 193: 99-103 https://doi.org/10.1111/j.1574-6968.2000.tb09409.x
  25. Haitani, Y., N. Awano, M. Yamazaki, M. Wada, S. Nakamori, and H. Takagi. 2006. Functional analysis of L-serine Oacetyltransferase from Corynebacterium glutamicum. FEMS Microbiol. Lett. 255: 156-163 https://doi.org/10.1111/j.1574-6968.2005.00068.x
  26. Hansmeier, N., A. Albersmeier, A. Tauch, T. Damberg, R. Ros, D. Anselmetti, A. Puhler, and J. Kalinowski. 2006. The surface (S)-layer gene cspB of Corynebacterium glutamicum is transcriptionally activated by a LuxR-type regulator and located on a 6 kb genomic island absent from the type strain ATCC 13032. Microbiology 152: 923-935 https://doi.org/10.1099/mic.0.28673-0
  27. Hansmeier, N., F. W. Bartels, R. Ros, D. Anselmetti, A. Tauch, A. Puhler, and J. Kalinowski. 2004. Classification of hyper-variable Corynebacterium glutamicum surface-layer proteins by sequence analyses and atomic force microscopy. J. Biotechnol.112: 177-193 https://doi.org/10.1016/j.jbiotec.2004.03.020
  28. Hayashi, M., H. Mizoguchi, N. Shiraishi, M. Obayashi, S. Nakagawa, J. Imai, S. Watanabe, T. Ota, and M. Ikeda. 2002. Transcriptome analysis of acetate metabolism in Corynebacterium glutamicum using a newly developed metabolic array. Biosci. Biotechnol. Biochem. 66: 1337-1344 https://doi.org/10.1271/bbb.66.1337
  29. Hayashi, M., J. Ohnishi, S. Mitsuhashi, Y. Yonetani, S. Hashimoto, and M. Ikeda. 2006. Transcriptome analysis reveals global expression changes in an industrial L-Iysine producer of Corynebacterium glutamicum. Biosci. Biotechnol. Biochem. 70: 546-550 https://doi.org/10.1271/bbb.70.546
  30. Hermann, T. 2003. Industrial production of amino acids by coryneform bacteria. J. Biotechnol. 104: 155-172 https://doi.org/10.1016/S0168-1656(03)00149-4
  31. Hwang, B. J., Y. Kim, H. B. Kim, H. J. Hwang, J. H. Kim, and H. S. Lee. 1999. Analysis of Corynebacterium glutamicum methionine biosynthetic pathway: Isolation and analysis of metB encoding cystathionine gamma-synthase. Mol. Cells 9: 300-308
  32. Hwang, B. J., H. J. Yeom, Y. Kim, and H. S. Lee. 2002. Corynebacterium glutamicum utilizes both transsulfuration and direct sulthydrylation pathways for methionine biosynthesis. J. Bacteriol. 184: 1277-1286 https://doi.org/10.1128/JB.184.5.1277-1286.2002
  33. Ikeda, M. and S. Nakagawa. 2003. The Corynebacterium glutamicum genome: Features and impacts on biotechnological processes. Appl. Microbiol. Biotechnol. 62: 99-109 https://doi.org/10.1007/s00253-003-1328-1
  34. Ikeda, M., J. Ohnishi, M. Hayashi, and S. Mitsuhashi. 2006. A genome-based approach to create a minimally mutated Corynebacterium glutamicum strain for efficient L-lysine production. J. Industrial. Microbiol. Biotechnol. 33: 610- 615 https://doi.org/10.1007/s10295-006-0104-5
  35. Ishige, T., M. Krause, M. Bott, V. F. Wendisch, and H. Sahm. 2003. The phosphate starvation stimulon of Corynebacterium glutamicum determined by DNA microarray analyses. J. Bacteriol. 185: 4519-4529 https://doi.org/10.1128/JB.185.15.4519-4529.2003
  36. Jakoby, M., L. Nolden, J. Meier-Wagner, R. Kramer, and A. Burkovski. 2000. AmtR, a global repressor in the nitrogen regulation system of Corynebacterium glutamicum. Mol. Microbiol. 37: 964-977 https://doi.org/10.1046/j.1365-2958.2000.02073.x
  37. Kalinowski, J., B. Bathe, D. Bartels, N. Bischoff, M. Bott, A. Burkovski, N. Dusch, L. Eggeling, B. J. Eikmanns, L. Gaigalat, A. Goesmann, M. Hartmann, K. Huthmacher, R. Kramer, B. Linke, A. C. McHardy, F. Meyer, B. Mockel, W. Pfefferle, A. Puhler, D. A. Rey, C. Ruckert, O. Rupp, H. Sahm, V. F. Wendisch, I. Wiegrabe, and A. Tauch. 2003. The complete Corynebacterium glutamicum ATCC 13032 genome sequence and its impact on the production of Laspartate- derived amino acids and vitamins. J. Biotechnol. 104: 5-25 https://doi.org/10.1016/S0168-1656(03)00154-8
  38. Kawaguchi, H., A. A. Vertes, S. Okino, M. Inui, and H. Yukawa. 2006. Engineering of a xylose metabolic pathway in Corynebacterium glutamicum. Appl. Environ. Microbiol. 72: 3418-3428 https://doi.org/10.1128/AEM.72.5.3418-3428.2006
  39. Kiefer, P., E. Heinzle, and C. Wittmann. 2002. Influence of glucose, fructose and sucrose as carbon sources on kinetics and stoichiometry of lysine production by Corynebacterium glutamicum. J. Industrial. Microbiol. Biotechnol. 28: 338-343 https://doi.org/10.1038/sj.jim.7000252
  40. Kim, H. J., T. H. Kim, Y. Kim, and H. S. Lee. 2004. Identification and characterization of glxR, a gene involved in regulation of glyoxylate bypass in Corynebacterium glutamicum. J. Bacteriol. 186: 3453-3460 https://doi.org/10.1128/JB.186.11.3453-3460.2004
  41. Kim, H. J., J. S. Park, H. S. Lee, and Y. Kim. 2002. Utilization of lacZ to isolate regulatory genes from Corynebacterium glutamicum. J. Microbiol. Biotechnol. 12: 336-339
  42. Kim, J. W., H. J. Kim, Y. Kim, M. S. Lee, and H. S. Lee. 2001. Properties of the Corynebacterium glutamicum metC gene encoding cystathionine beta-lyase. Mol. Cells 11: 220- 225
  43. Kocan, M., S. Schaffer, T. Ishige, U. Sorger-Herrmann, V. F. Wendisch, and M. Bott. 2006. Two-component systems of Corynebacterium glutamicum: Deletion analysis and involvement of the PhoS-PhoR system in the phosphate starvation response. J. Bacteriol. 188: 724-732 https://doi.org/10.1128/JB.188.2.724-732.2006
  44. Koch, D. J., C. Ruckert, A. Albersmeier, A. T. Huser, A. Tauch, A. Puhler, and J. Kalinowski. 2005. The transcriptional regulator SsuR activates expression of the Corynebacterium glutamicum sulphonate utilization genes in the absence of sulphate. Mol. Microbiol. 58: 480-494 https://doi.org/10.1111/j.1365-2958.2005.04836.x
  45. Koch, D. J., C. Ruckert, D. A. Rey, A. Mix, A. Puhler, and J. Kalinowski. 2005. Role of the ssu and seu genes of Corynebacterium glutamicum ATCC 13032 in utilization of sulfonates and sulfonate esters as sulfur sources. Appl. Environ. Microbiol, 71: 6104-6114 https://doi.org/10.1128/AEM.71.10.6104-6114.2005
  46. Kramer, R., C. Lambert, C. Hoischen, and H. Ebbighausen. 1990. Uptake of glutamate in Corynebacterium glutamicum. 1. Kinetic properties and regulation by internal pH and potassium. Eur. J. Biochem. 194: 929-935 https://doi.org/10.1111/j.1432-1033.1990.tb19488.x
  47. Kromer, J. O., E. Heinzle, H. Schroder, and C. Wittmann. 2006. Accumulation of homolanthionine and activation of a novel pathway for isoleucine biosynthesis in Corynebacterium glutamicum McbR deletion strains. J .Bacteriol. 188: 609-618 https://doi.org/10.1128/JB.188.2.609-618.2006
  48. Kromer, J. O., O. Sorgenfrei, K. Klopprogge, E. Heinzle, and C. Wittmann. 2004. In-depth profiling of lysine-producing Corynebacterium glutamicum by combined analysis of the transcriptome, metabolome, and fluxome. J. Bacteriol. 186: 1769-1784 https://doi.org/10.1128/JB.186.6.1769-1784.2004
  49. Kronemeyer, W., N. Peekhaus, R. Kramer, H. Sahm, and L. Eggeling. 1995. Structure of the gluABCD cluster encoding the glutamate uptake system of Corynebacterium glutamicum. J. Bacteriol. 177: 1152-1158 https://doi.org/10.1128/jb.177.5.1152-1158.1995
  50. Krug, A., V. F. Wendisch, and M. Bott. 2005. Identification of AcnR, a TetR-type repressor of the aconitase gene acn in Corynebacterium glutamicum. J. Biol. Chem. 280: 585-595 https://doi.org/10.1074/jbc.M408271200
  51. Lange, C., D. Rittmann, V. F. Wendisch, M. Bott, and H. Sahm. 2003. Global expression profiling and physiological characterization of Corynebacterium glutamicum grown in the presence of L-valine. Appl. Environ. Microbiol. 69: 2521-2532 https://doi.org/10.1128/AEM.69.5.2521-2532.2003
  52. Lee, H.-S. 2005. Sulfur metabolism of Corynebacterium glutamicum, pp. 351-376. In Eggeling L. and Bott, M. (eds.), Handbook of Corynebacterium glutamicum. CRC Press, Boca Raton, U.S.A
  53. Lee, H. S. and B. J. Hwang. 2003. Methionine biosynthesis and its regulation in Corynebacterium glutamicum: Parallel pathways of transsulfuration and direct sulfhydrylation. Appl. Microbiol. Biotechnol. 62: 459-467 https://doi.org/10.1007/s00253-003-1306-7
  54. Lee, H.-S. and A. J. Sinskey. 1994. Molecular characterization of aceB, a gene encoding malate synthase in Corynebacterium glutamicum. J. Microbiol. Biotechnol. 4: 256-263
  55. Letek, M., N. Valbuena, A. Ramos, E. Ordonez, J. A. Gil, and L. M. Mateos. 2006. Characterization and use of catabolite-repressed promoters from gluconate genes in Corynebacterium glutamicum. J. Bacteriol. 188: 409-423 https://doi.org/10.1128/JB.188.2.409-423.2006
  56. Leuchtenberger, W., K. Huthmacher, and K. Drauz. 2005. Biotechnological production of amino acids and derivatives:Current status and prospects. Appl. Microbiol. Biotechnol. 69: 1-8 https://doi.org/10.1007/s00253-005-0155-y
  57. Liebl, W., R. Klamer, and K. H. Schleifer. 1989. Requirement of chelating compounds for the growth of Corynebacterium glutamicum in synthetic media. Appl. Microbiol. Biotechnol. 32: 205-210 https://doi.org/10.1007/BF00165889
  58. Marienhagen, J., N. Kennerknecht, H. Sahm, and L. Eggeling. 2005. Functional analysis of all aminotransferase proteins inferred from the genome sequence of Corynebacterium glutamicum. J. Bacteriol. 187: 7639-7646 https://doi.org/10.1128/JB.187.22.7639-7646.2005
  59. Marx, A., A. A. de Graaf, W. Wiechert, L. Eggeling, and H. Sahm. 1996. Determination of the fluxes in the central metabolism of Corynebacterium glutamicum by nuclear magnetic resonance spetroscopy combined with metabolite balancing. Biotech. Bioeng. 49: 111-129 https://doi.org/10.1002/(SICI)1097-0290(19960120)49:2<111::AID-BIT1>3.0.CO;2-T
  60. Marx, A., B. J. Eikmanns, H. Sahm, A. A. de Graaf, and L. Eggeling. 1999. Response of the central metabolism in Corynebacterium glutamicum to the use of an NADHdependent glutamate dehydrogenase. Metab. Eng. 1: 35-48 https://doi.org/10.1006/mben.1998.0106
  61. Marx, A., S. Hans, B. Mockel, B. Bathe, and A. A. de Graaf. 2003. Metabolic phenotype of phosphoglucose isomerase mutants of Corynebacterium glutamicum. J. Biotechnol. 104: 185-197 https://doi.org/10.1016/S0168-1656(03)00153-6
  62. Marx, A., K. Striegel, A. A. de Graaf, H. Sahm, and L. Eggeling. 1997. Response of the central metabolism of Corynebacterium glutamicum to different flux burdens. Biotech. Bioeng. 56: 168-180 https://doi.org/10.1002/(SICI)1097-0290(19971020)56:2<168::AID-BIT6>3.0.CO;2-N
  63. Marx, A., V. F. Wendisch, R. Kelle, and S. Buchholz. 2005. Towards Integration of Biorefinery and Microbial Amino Acid Production, pp. 201-216. In Kamm, B., Gruber, P. R. and Kamm, M. (eds.), Biorefmeries - Industrial Processes and Products: Status Quo and Future Directions. WileyVCH, Weinheim, Germany
  64. Meier-Wagner, J., L. Nolden, M. Jakoby, R. Siewe, R. Kramer, and A. Burkovski. 2001. Multiplicity of ammonium uptake systems in Corynebacterium glutamicum: Role of Amt and AmtB. Microbiology 147: 135-143 https://doi.org/10.1099/00221287-147-1-135
  65. Merkens, H., G. Beckers, A. Wirtz, and A. Burkovski. 2005. Vanillate Metabolism in Corynebacterium glutamicum. Curr. Microbiol. 51
  66. Morbach, S., C. Junger, H. Sahm, and L. Eggeling. 2000. Attenuation control of ilvBNC in Corynebacterium glutamicum:Evidence of leader peptide formation without the presence of a ribosome binding site. J. Biosci. Bioeng. 90: 501-507 https://doi.org/10.1016/S1389-1723(01)80030-X
  67. Muffler, A., S. Bettermann, M. Haushalter, A. Horlein, U. Neveling, M. Schramm, and O. Sorgenfrei. 2002. Genomewide transcription profiling of Corynebacterium glutamicum after heat shock and during growth on acetate and glucose. J. Biotechnol. 98: 255-268 https://doi.org/10.1016/S0168-1656(02)00136-0
  68. Netzer, R., M. Krause, D. Rittmann, P. G. Peters-Wendisch, L. Eggeling, V. F. Wendisch, and H. Sahm. 2004. Roles of pyruvate kinase and malic enzyme in Corynebacterium glutamicum for growth on carbon sources requiring gluconeogenesis. Arch. Microbiol. 182: 354-363 https://doi.org/10.1007/s00203-004-0710-4
  69. Netzer, R., P. Peters-Wendisch, L. Eggeling, and H. Sahm. 2004. Cometabolism of a nongrowth substrate: L-serine utilization by Corynebacterium glutamicum. Appl. Environ. Microbiol. 70: 7148-7155 https://doi.org/10.1128/AEM.70.12.7148-7155.2004
  70. Niebisch, A., A. Kabus, C. Schultz, B. Weil, and M. Bott. 2006. Corynebacterial protein kinase G controls 2-oxoglutarate dehydrogenase activity via the phosphorylation status of the OdhI protein. J. BioI. Chem. 281: 12300-12307 https://doi.org/10.1074/jbc.M512515200
  71. Nolden, L., G Beckers, B. Mockel, W. Pfefferle, K. M. Nampoothiri, R. Kramer, and A. Burkovski. 2000. Urease of Corynebacterium glutamicum: Organization of corresponding genes and investigation of activity. FEMS Microbiol. Lett. 189: 305-310 https://doi.org/10.1111/j.1574-6968.2000.tb09248.x
  72. Nolden, L., C. E. Ngouoto-Nkili, A. K. Bendt, R. Kramer, and A. Burkovski. 2001. Sensing nitrogen limitation in Corynebacterium glutamicum: The role of glnK and glnD. Mol. Microbiol. 42: 1281-1295 https://doi.org/10.1046/j.1365-2958.2001.02694.x
  73. Ohnishi, J., R. Katahira, S. Mitsuhashi, S. Kakita, and M. Ikeda. 2005. A novel gnd mutation leading to increased Llysine production in Corynebacterium glutamicum. FEMS Microbiol. Lett. 242: 265-274 https://doi.org/10.1016/j.femsle.2004.11.014
  74. Ohnishi, J., S. Mitsuhashi, M. Hayashi, S. Ando, H. Yokoi, K. Ochiai, and M. Ikeda. 2002. A novel methodology employing Corynebacterium glutamicum genome information to generate a new L-lysine-producing mutant. Appl. Microbiol. Biotechnol. 58: 217-223 https://doi.org/10.1007/s00253-001-0883-6
  75. Pallerla, S. R., S. Knebel, T. Polen, P. Klauth, J. Hollender, V. F. Wendisch, and S. M. Schoberth. 2005. Formation of volutin granules in Corynebacterium glutamicum. FEMS Microbiol. Lett. 243: 133-140 https://doi.org/10.1016/j.femsle.2004.11.047
  76. Park, S. D., J. Y. Lee, Y. Kim, J. H. Kim, and H. S. Lee. 1998. Isolation and analysis of metA, a methionine biosynthetic gene encoding homoserine acetyltransferase in Corynebacterium glutamicum. Mol. Cells 8: 286- 294
  77. Peters-Wendisch, P., M. Stolz, H. Etterich, N. Kennerknecht, H. Sahm, and L. Eggeling. 2005. Metabolic engineering of Corynebacterium glutamicum for L-serine production. Appl. Environ. Microbiol. 71: 7139-7144 https://doi.org/10.1128/AEM.71.11.7139-7144.2005
  78. Peters- Wendisch, P. G, B. Schiel, V. F. Wendisch, E. Katsoulidis, B. Mockel, H. Sahm, and B. J. Eikmanns. 2001. Pyruvate carboxylase is a major bottleneck for glutamate and lysine production by Corynebacterium glutamicum. J. Mol. Microbiol. Biotechnol. 3: 295-300
  79. Polen, T. and V. F. Wendisch. 2004. Genomewide expression analysis in amino acid-producing bacteria using DNA microarrays. Appl. Biochern. Biotechnol. 118: 215-232 https://doi.org/10.1385/ABAB:118:1-3:215
  80. Radmacher, E., K. C. Stansen, G S. Besra, L. J. Alderwick, W. N. Maughan, G Hollweg, H. Sahm, V. F. Wendisch, and L. Eggeling. 2005. Ethambutol, a cell wall inhibitor of Mycobacterium tuberculosis, elicits L-glutamate efflux of Corynebacterium glutamicum. Microbiology 151: 1359-1368 https://doi.org/10.1099/mic.0.27804-0
  81. Reinscheid, D. J., B. J. Eikmanns, and H. Sahm. 1994. Characterization of the isocitrate lyase gene from Corynebacterium glutamicum and biochemical analysis of the enzyme. J. Bacteriol. 176: 3474-3483 https://doi.org/10.1128/jb.176.12.3474-3483.1994
  82. Reinscheid, D. J., B. J. Eikmanns, and H. Sahm. 1994. Malate synthase from Corynebacterium glutamicum: Sequence analysis of the gene and biochemical characterization of the enzyme. Microbiology 140: 3099-3108 https://doi.org/10.1099/13500872-140-11-3099
  83. Reinscheid, D. J., S. Schnicke, D. Rittmann, U. Zahnow, H. Sahm, and B. J. Eikmanns. 1999. Cloning, sequence analysis, expression and inactivation of the Corynebacterium glutamicum pta-ack operon encoding phosphotransacetylase and acetate kinase. Microbiology 145: 503-513 https://doi.org/10.1099/13500872-145-2-503
  84. Rey, D. A, S. S. Nentwich, D. J. Koch, C. Ruckert, A Puhler, A Tauch, and J. Kalinowski. 2005. The McbR repressor modulated by the effector substance S-adenosylhomocysteine controls directly the transcription of a regulon involved in sulphur metabolism of Corynebacterium glutamicum ATCC 13032. Mol. Microbiol. 56: 871-887 https://doi.org/10.1111/j.1365-2958.2005.04586.x
  85. Riedel, C., D. Rittmann, P. Dangel, B. Mockel, S. Petersen, H. Sahm, and B. J. Eikmanns. 2001. Characterization of the phosphoenolpyruvate carboxykinase gene from Corynebacterium glutamicum and significance of the enzyme for growth and amino acid production. J. Mol. Microbiol. Biotechnol. 3: 573-583
  86. Rittmann, D., S. Schaffer, V. F. Wendisch, and H. Sahm. 2003. Fructose-1,6-bisphosphatase from Corynebacterium glutamicum: Expression and deletion of the fbp gene and biochemical characterization of the enzyme. Arch. Microbiol. 180: 285-292 https://doi.org/10.1007/s00203-003-0588-6
  87. Rittmann, D., U. Sorger-Herrmann, and V. F. Wendisch. 2005. Phosphate starvation-inducible gene ushA encodes a 5' nucleotidase required for growth of Corynebacterium glutamicum on media with nucleotides as the phosphorus source. Appl. Environ. Microbiol. 71: 4339-4344 https://doi.org/10.1128/AEM.71.8.4339-4344.2005
  88. Ruckert, C., A. Puhler, and J. Kalinowski. 2003. Genomewide analysis of the L-methionine biosynthetic pathway in Corynebacterium glutamicum by targeted gene deletion and homologous complementation. J. Biotechnol. 104: 213-228 https://doi.org/10.1016/S0168-1656(03)00158-5
  89. Schaffer, S. and A. Burkovski. 2005. Proteomics, pp. 99- 118. In L. Eggeling and M. Bott (eds.), Handbook of Corynebacterium glutamicum. CRC Press, Boca Raton, Florida, U.S.A
  90. Schmid, R., E. M. Uhlemann, L. Nolden, G. Wersch, R. Hecker, T. Hermann, A. Marx, and A. Burkovski. 2000. Response to nitrogen starvation in Corynebacterium glutamicum. FEMS Microbiol. Lett. 187: 83-88 https://doi.org/10.1111/j.1574-6968.2000.tb09141.x
  91. Silberbach, M. and A. Burkovski. 2006. Application of global analysis techniques to Corynebacterium glutamicum: New insights into nitrogen regulation. J. Biotechnol. in press
  92. Silberbach, M., M. Schafer, A. T. Huser, J. Kalinowski, A. Puhler, R. Kramer, and A. Burkovski. 2005. Adaptation of Corynebacterium glutamicum to ammonium limitation: A global analysis using transcriptome and proteome techniques. Appl. Environ. Microbiol. 71: 2391-2402 https://doi.org/10.1128/AEM.71.5.2391-2402.2005
  93. Simic, P., J. Willuhn, H. Sahm, and L. Eggeling. 2002. Identification of glyA (encoding serine hydroxymethyltransferase) and its use together with the exporter ThrE to increase L-threonine accumulation by Corynebacterium glutamicum. Appl. Environ. Microbiol. 68: 3321-3327 https://doi.org/10.1128/AEM.68.7.3321-3327.2002
  94. Soual-Hoebeke, E., C. de Sousa-D'Auria, M. Chami, M. F. Baucher, A. Guyonvarch, N. Bayan, K. Salim, and G Leblon. 1999. S-layer protein production by Corynebacterium strains is dependent on the carbon source. Microbiology 145: 3399-3408 https://doi.org/10.1099/00221287-145-12-3399
  95. Stansen, C., D. Uy, S. Delaunay, L. Eggeling, J. L. Goergen, and V. F. Wendisch. 2005. Characterization of a Corynebacterium glutamicum lactate utilization operon induced during temperature-triggered glutamate production. Appl. Environ. Microbiol. 71: 5920-5928 https://doi.org/10.1128/AEM.71.10.5920-5928.2005
  96. Strosser, J., A. Ludke, S. Schaffer, R. Kramer, and A. Burkovski. 2004. Regulation of GlnK activity: Modification, membrane sequestration and proteolysis as regulatory principles in the network of nitrogen control in Corynebacterium glutamicum. Mol. Microbiol. 54: 132-147 https://doi.org/10.1111/j.1365-2958.2004.04247.x
  97. Tesch, M., A. A. de Graaf, and H. Sahm. 1999. In vivo fluxes in the ammonium-assimilatory pathways in Corynebacterium glutamicum studied by $^{15}N$ nuclear magnetic resonance. Appl. Environ. Microbiol. 65: 1099-1109
  98. Trotschel, C., S. Kandirali, P. Diaz-Achirica, A. Meinhardt, S. Morbach, R. Kramer, and A. Burkovski. 2003. GltS, the sodium-coupled L-glutamate uptake system of Corynebacterium glutamicum: Identification of the corresponding gene and impact on L-glutamate production. Appl. Microbiol. Biotechnol. 60: 738-742 https://doi.org/10.1007/s00253-002-1170-x
  99. van der Ploeg, J. R., E. Eichhorn, and T. Leisinger. 2001. Sulfonate-sulfur metabolism and its regulation in Escherichia coli. Arch. Microbiol. 176: 1-8 https://doi.org/10.1007/s002030100298
  100. Vrljic, M., H. Sahm, and L. Eggeling. 1996. A new type of transporter with a new type of cellular function: L-lysine export from Corynebacterium glutamicum. Mol. Microbiol. 22: 815-826 https://doi.org/10.1046/j.1365-2958.1996.01527.x
  101. Wendisch, V. F. 2003. Genome-wide expression analysis in Corynebacterium glutamicum using DNA microarrays. J. Biotechnol. 104: 273-285 https://doi.org/10.1016/S0168-1656(03)00147-0
  102. Wendisch, V. F. and M. Bott. 2005. Phosphorus metabolism of Corynebacterium glutamicum, pp. 377-396 In Eggeling, L. and Bott, M. (eds.), Handbook of Corynebacterium glutamicum. CRC Press, Boca Raton, U.S.A
  103. Wendisch, V. F, M. Bott, J. Kalinowski, M. Oldiges, and W. Wiechert. 2006. Emerging Corynebacterium glutamicum systems biology. J. Biotechnol. 124: 74-92 https://doi.org/10.1016/j.jbiotec.2005.12.002
  104. Wendisch, V. F, A. A. de Graaf, H. Sahm, and B. J. Eikmanns. 2000. Quantitative determination of metabolic fluxes during coutilization of two carbon sources: comparative analyses with Corynebacterium glutamicum during growth on acetate and/or glucose. J. Bacteriol. 182: 3088-3096 https://doi.org/10.1128/JB.182.11.3088-3096.2000
  105. Wendisch, V. F., M. Spies, D. J. Reinscheid, S. Schnicke, H. Sahm, and B. J. Eikmanns. 1997. Regulation of acetate metabolism in Corynebacterium glutamicum: Transcriptional control of the isocitrate lyase and malate synthase genes. Arch. Microbiol. 168: 262-269 https://doi.org/10.1007/s002030050497
  106. Wennerhold, J. and M. Bott. 2006. The DtxR regulon of Corynebacterium glutamicum. J. Bacteriol. 188: 2907-2918 https://doi.org/10.1128/JB.188.8.2907-2918.2006
  107. Wennerhold, J., A. Krug, and M. Bott. 2005. The AraCtype regulator RipA represses aconitase and other iron proteins from Corynebacterium under iron limitation and is itself repressed by DtxR. J. Biol. Chem. 280: 40500-40508 https://doi.org/10.1074/jbc.M508693200