• Title/Summary/Keyword: amber

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An analysis of the arm-type site binding domain of bacteriophage .lambda. integrase

  • Cho, Eun-Hee
    • Journal of Microbiology
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    • v.33 no.2
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    • pp.165-170
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    • 1995
  • The 356 amino acid long lambda integrase protein of bacteriophage .lambda. constains two autonomous DNA binding domains with distinct sequence specificities. The amino terminal domain of integrase is implicated to bind to the arm-type sequences and the carboxyl domain interacts with the coretype sequencess. As a first step to understand the molecular mechanism of the integrase-DNA interaction at the arm-type site, the int(am)94 gene carrying an amber mutation at the 94th codon of the int was cloned under the control of the P$\_$tac/ promoter and the lacI$\_$q/ gene. The Int(am)94 mutant protein of amino terminal 93 amino acid residues can be produced at high level from a suppressor free strain harboring the plasmid pInt(am)94. The arm-type binding activity of Int(am)94 were measured in vivo and in vitro. A comparison of the arm-type binding properties of the wild-type integrase and the truncated Int(am)94 mutant indicated that the truncated fragment containing 93 amino acid residues carry all the determinants for DNA binding at the arm-type sites.

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An Experimental Study on Physical and Mechanical Properties of Steel Fiber Reinforced Concrete Containing Waste Glass (폐유리를 혼입한 강섬유보강 콘크리트의 물리ㆍ역학적 특성에 관한 실험적 연구)

  • 박승범;이봉춘;조광연;이택우
    • Proceedings of the Korea Concrete Institute Conference
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    • 2002.05a
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    • pp.903-908
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    • 2002
  • The production of waste glasses has been increased with the development of industry. The utilization of waste glass for concrete can cause the concrete to be cracked and to be weakened due to an expansion by alkali-silica reaction(ASR). When used the fibers with waste glass, there is an effect on reduction of expansion and strength loss due to ASR between the alkali in the cement paste and the silica in the waste glass. In this study, we conducted basic experimental research to analyze the possibilities of recycling of amber waste glass as fine aggregates for steel fiber reinforced concrete. Test results of fresh concrete. slump is decreased because grain shape is angular and air content is increased due to involving small size particles so much in waste glasses. Also. tensile and flexural strengths increased as the content of steel fibers increased. In conclusion, the content of waste glass below 40% is reasonable and usage of pertinent admixture is necessary to obtain workability or air content.

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Acute Onset of Intracerebral Hemorrhage due to Autonomic Dysreflexia

  • Eker, Amber;Yigitoglu, Pembe Hare;Ipekdal, H. Ilker;Tosun, Aliye
    • Journal of Korean Neurosurgical Society
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    • v.55 no.5
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    • pp.277-279
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    • 2014
  • Autonomic dysreflexia is a clinical emergency syndrome of uncontrolled sympathetic output that can occur in patients who have a history of spinal cord injury. Despite its frequency in spinal cord injury patients, central nervous system complications are very rare. We report a man with traumatic high level incomplete spinal cord injury who suffered hypertensive right thalamic hemorrhage secondary to an episode of autonomic dysreflexia. Prompt recognition and removal of the triggering factor, the suprapubic catheter obstruction which led to hypertensive attack, the patient had a favorable functional outcome after the resorption of the hematoma and effective rehabilitation programme.

Production and Purification of Pepsin Inhibitor from Actinomycetes GF 155-2 (Actinomycetes GF 155-2에 의한 pepsin 저해물질의 생산 및 정제)

  • 박석규;성낙계;이상원
    • Microbiology and Biotechnology Letters
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    • v.17 no.2
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    • pp.121-125
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    • 1989
  • Actinomycetes GF 155-2, which produced an extracellular pepsin inhibitor, was isolated from soil samples. Optimal conditions of inhibitor production by flask-shacking culture were 2% glucose, 0.7% polypeptone, initial pH 1.0, culture time 60 hours and temperature 30%. Effect of in-organic salts was not observed. About 5mg of colorless crystalline inhibitor was obtained from 5L culture broth in jar tormentor by means of ammonium sulfate precipitation, methanol extraction, and column chromatographies on Amberlite IR-120, XAD-2 and silicagel 60.

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Effects of Processed Slag as Glassmaking Raw Material (on Soda-Lime Glass) (개질 고로 슬래그에 관한 연구 (유리 제조에 관한 연구))

  • 양중식
    • Journal of the Korean Ceramic Society
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    • v.18 no.2
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    • pp.112-118
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    • 1981
  • A study was made on the processing of domestic blast furnace slag by flotation and chemical purification for the use of slag as a raw material in making soda lime glasses. Feasibility study has been made for the use of reprocessing slag as a source material for both coloring and chemical components (such as CaO, $Al_2O_3$, MgO and etc.) in the glass making process. Chemical composition of chemically purified slags ranges; $SiO_2$ 34.5~37.5, $Al_2O_3$, 16.2~14.1, $Fe_2O_3$ 0.33~0.14, CaO 34.5~38.8, MgO 4.0~5.2, NmO 0.16~0.39, $TiO_2$ 0.23~0.35, S 0.08~0.42, ignition loss 3.3~8.4 and others 0.48~0.51%. It was found that either amber or greenish color could be easily obtained with the addition of salg up to 24%, however the slag addition to glass batches much impaired the transmitancy of glass products, thus a glass made with 7% slag addition showed 82% in transmitancy value at 510 nm.

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A Study on the Scythian Torque

  • Kim, Moon-Ja
    • The International Journal of Costume Culture
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    • v.6 no.2
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    • pp.69-82
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    • 2003
  • The Scythians had a veritable passion for adornment, delighting in decorating themselves no less than their horses and belongings. Their love of jewellery was expressed at every turn. The most magnificent pieces naturally come from the royal tombs. In the area of the neck and chest the Scythian had a massive gold Torques, a symbol of power, made of gold, turquoise, cornelian coral and even amber. The entire surface of the torque, like that of many of the other artefacts, is decorated with depictions of animals. Scythian Torques are worn with the decorative terminals to the front. It was put a Torque on, grasped both terminals and placed the opening at the back of the neck. It is possible the Torque signified its wearer's religious leadership responsibilities. Scythian Torques were divided into several types according to the shape, Torque with Terminal style, Spiral style, Layers style, Crown style, Crescent-shaped pectoral style.

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Determination of Optimal Phase Split and Offset for the Synchronization of Traffic Signals in the CBD of Seoul (서울시(市) 신호체제의 적정 phase split 과 연쇄화를 위한 최적 offset)

  • Park, Gyeong-Su
    • Journal of Korean Institute of Industrial Engineers
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    • v.3 no.1
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    • pp.49-53
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    • 1977
  • The coordinated control of the traffic signals of adjacent intersections can reduce delays, relative number of stops and congestions in the coordinated traffic area. The road capacity can be increased to a certain extend because the stopping and starting of vehicles facing red traffic lights can be avoided in many instances due to the progression established along an artery. However, if traffic centers or leaves the main flow in irregular volumes on the intermediate road section, a coordination of traffic signals is unnecessary and may even be harmful. Therefore, a computer simulation model to simulate and predict the effectiveness of a synchronized traffic signal system in the CBD of Seoul was developed and alternative policy variables, such as cycle time, offsets, phase splits, to be fed into the simulation model had to be generated. This is a report of (1) the development of a heuristic algorithm for the determination of phase splits when there are amber periods specifically reserved for left turns and (2) the computerization of time-space diagramming.

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Assessment of material analysis for Halogen free flame retardant concentric neutral type Power cable (난연성 동심중성선 전력 케이블의 재료에 대한 평가 기술)

  • Soh, Jin-Joong;Shim, Dae-Sub
    • Proceedings of the KIEE Conference
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    • 2002.07c
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    • pp.1460-1462
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    • 2002
  • The test methods on materials of electrical power cable are used in accordance with 25 specifications of IEC, ASTM, ICEA, KS and KEPCO Spec. The validation of test methods were checked and stablished by solving the problems occurred during the test. These tests are flammability, degree of acidity of gases, amount of halogen acid gas, smoke density, oxygen index, amount of ion in semiconductor, void, amber, contaminant and water tree, crosslinked density. Then the performance of power cable made by A, B, C and D company were evaluated and compared.

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구장산술의 원판과 영문번역판의 간격

  • 유인영
    • Journal for History of Mathematics
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    • v.16 no.4
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    • pp.1-14
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    • 2003
  • The Nine Chapters by Liu Hui(劉徽) in the three kingdoms(220∼265 AD) is the fundamental source of the traditional Chinese Mathematics and has not only remained the lighthouse of the traditional Chinese Mathematics over the last 2000 years, but also has exerted a profound influence on the development of mathematics in the neigh-bouring countries and regions. At last it also translated into English. In this paper, some differences between the Orignal and the New Translated Nine Chapters will be introduced and a problem of Mathematical Treatise in Nine Sections (Shushu Jiuzhang, 수서구장, 1247) of Qin Jiushao(진구소, 1202∼1261 AD) in the Song (송, 960∼1261 AD) Dynasty It looks like an insect in the amber but not error.

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The Interaction of Phenylthiourea Derivatives as Catechol Oxidase Inhibitors by Molecular Mechanics Simulation (페닐티오우레아 유도체와 카테콜 산화효소와의 상호작용에 대한 분자역학적 모의실험)

  • Park, Kyung Lae
    • YAKHAK HOEJI
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    • v.60 no.2
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    • pp.78-84
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    • 2016
  • N-Phenylthiourea derivatives and catechol oxidase receptor complex was studied using molecular mechanics method. The starting structure was adopted from the protein databank and the calculation of energy minimization and molecular dynamics was performed with AMBER package. The molecular dynamics showed that the simulation time span of 20 ns was long enough to observe the interaction profile and stationary ligand-receptor configuration in the complex. The conformation of the ligand was related to the interaction to the receptor and the efficacy was also interpreted in this context.