Journal of Microbiology

The Microbiological Society of Korea (한국미생물학회)
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An analysis of the arm-type site binding domain of bacteriophage .lambda. integrase

  • Cho, Eun-Hee (Department of Science Education, Chosun University)
  • Published : 1995.06.01
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Abstract

The 356 amino acid long lambda integrase protein of bacteriophage .lambda. constains two autonomous DNA binding domains with distinct sequence specificities. The amino terminal domain of integrase is implicated to bind to the arm-type sequences and the carboxyl domain interacts with the coretype sequencess. As a first step to understand the molecular mechanism of the integrase-DNA interaction at the arm-type site, the int(am)94 gene carrying an amber mutation at the 94th codon of the int was cloned under the control of the P$\_$tac/ promoter and the lacI$\_$q/ gene. The Int(am)94 mutant protein of amino terminal 93 amino acid residues can be produced at high level from a suppressor free strain harboring the plasmid pInt(am)94. The arm-type binding activity of Int(am)94 were measured in vivo and in vitro. A comparison of the arm-type binding properties of the wild-type integrase and the truncated Int(am)94 mutant indicated that the truncated fragment containing 93 amino acid residues carry all the determinants for DNA binding at the arm-type sites.

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References

  1. J. Mol. Biol. v.192 Mutational analysis of integrase arm-type binding sites of bacteriophage lambda Bauer,C.E.;S.D.Hesse;R.I.Gumport;J.F.Gardner
  2. J. Bacteriol. v.169 Mutational analysis of the lambda int gene:DNA sequence of domminant mutations Bear,S.E.;J.B.Clemens;L.W.Enquist;R.J.Zagursky
  3. Genetics v.114 General selection for specific DNA-binding activities Benson,N.;P.Sugiono;S.Bass;L.V.Nendelman;P.Younderian
  4. Cell v.35 The mechanism of phage λ site-specific recombination:site-specific breakage of DNA by Int topoisomerase Craig,N.L.;H.A.Nash
  5. J. Mol. Biol. v.194 Systematic method for the detection of potential λ Cro-like DN-binding region in proteins Dodd,L.B.;J.B.Egan
  6. Science v.233 Multiple DNA-protein interactions governing high-precision DNA transactions Echols,H.
  7. EMBO J. v.12 Complementation on bacteriophage lambda integrase mutants:evidence for an intersubunit active site Han,Y.W.;R.I.Gumport;J.F.Gardner
  8. J. Mol. Biol. v.235 Mapping the functional domains of bacteriophage lambda integrase protein Han,Y.W.;R.I.Gumport;J.F.Gardner
  9. J. Mol. Biol. v.253 The bacteriophage λ int gene product Kikuch,A.;H.A.Nash
  10. Cold Spring Harbor Symp. Quant. Biol. v.43 Integrative recombination of bacteriophage λ:Requirement for supertwisted DNA in vivo and characterization of Int Kikuchi,Y.;H.A.Nash
  11. J. Mol. Biol. v.204 Bacteriophage lambda site-specific recombination process with a defined order of strand exchange Kitts,P.A.;H.A.Nash
  12. Ann. Rev. Biochem v.58 Dynamic, structural, and regulatory aspects of λ site-specific recombination Landy,A.
  13. J. Bacteriol. v.172 Genetic analysis of bacteriophage λ integrase interactions with arm-type attachment site sequences Lee,E.C.;R.I.Gumport;J.F.Gardner
  14. Ph.D. thesis. Univ. of Illinois A genetic analysis of bacteriophage lambda integrase-core site interactions MacWilliams,M.P.
  15. Experimental techniques in Bacterial Genetics Maloy,S.
  16. Cell v.54 Autonomous DNA binding domains of λ integrase recognize different sequence families Moitoso de Vargas,N.;C.A.Pargellis;N.M.Hasan;E.W.Bushman;Landy,A.
  17. PNAS v.79 Bacteriophage λ int protein recognizes two calsses of sequence in the phage at site:characterization of arm-type sites Ross,W.;A.Landy
  18. Cell v.33 Patterns of λ Int recognition in the regions of strand exchange Ross,W.;A.Landy
  19. Molecular in the cloning:a laboratory manual(2nd ed.) Sambrook,J.;E.F.Fritsch;T.Maniatis
  20. Quart. Rev. Biophy. v.23 Structural studies of protein-nucleic acid interaction:the sources of sequence-specific binding Steitz,T.A.
  21. J. Mol. Biol. v.195 Protein-protein interactions in a higher-order structure direct lambda site-specific recombination Thompson,J.F.;L.Moitoso de Vargas;S.E.Skinner;A.Landy
  22. Cell v.50 Cellular foctors couple recombination with growth phase:characterization of a new component in the λ site-specific recombination pathway Thompson,J.F.;L.Moitoso de Vargas;C.Koch;R.Kahmann;A.Landy