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Identification, sequence characterization and expression analysis of the arginine kinase gene in response to laminarin challenge from the Oriental land snail, Nesiohelix samarangae (동양달팽이(Nesiohelix samarangae)의 arginine kinase 유전자 분석 및 발현 패턴에 관한 연구)

  • Jeong, Ji Eun;Lee, Yong Seok
    • The Korean Journal of Malacology
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    • v.29 no.3
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    • pp.171-179
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    • 2013
  • Arginine kinase (ArK) is known to play an important role in most invertebrates the level of ATP by phosphorylation of phosphagens in cell and immuninty in living organisms. ArK has been identified in many kinds of organisms ranging from invertebrate to vertebrate. However, no ArK gene has been cloned and investigated from N. samarangae. This leads us to identify ArK cDNA (NsArK) from the expressed sequence tag (EST) sequencing of N. samarangae. Sequence analysis indicated that the coding region of 1,065 bp contains 355 amino acid residues. Molecular phylogenetic analysis shows that NsArK had very high similarities with mollusca and arthropoda. In an attempt to investigate a potential role of NsArK in the digestive gland of N. samarangae, expression patterns were analyzed. RT-PCR analsysis shows that NsArK mRNA is induced in the rane of 1.2 fold at 6 hr by laminarin when compared with the control. The immunnologial and physiological role of NsArK remains to be further investigated in N. samarangae.

Novel reassortant 2.3.4.4B H5N6 highly pathogenic avian influenza viruses circulating among wild, domestic birds in Xinjiang, Northwest China

  • Zhang, Qian;Mei, Xindi;Zhang, Cheng;Li, Juan;Chang, Nana;Aji, Dilihuma;Shi, Weifeng;Bi, Yuhai;Ma, Zhenghai
    • Journal of Veterinary Science
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    • v.22 no.4
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    • pp.43.1-43.10
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    • 2021
  • Background: The H5 avian influenza viruses (AIVs) of clade 2.3.4.4 circulate in wild and domestic birds worldwide. In 2017, nine strains of H5N6 AIVs were isolated from aquatic poultry in Xinjiang, Northwest China. Objectives: This study aimed to analyze the origin, reassortment, and mutations of the AIV isolates. Methods: AIVs were isolated from oropharyngeal and cloacal swabs of poultry. Identification was accomplished by inoculating isolates into embryonated chicken eggs and performing hemagglutination tests and reverse transcription polymerase chain reaction (RT-PCR). The viral genomes were amplified with RT-PCR and then sequenced. The sequence alignment, phylogenetic, and molecular characteristic analyses were performed by using bioinformatic software. Results: Nine isolates originated from the same ancestor. The viral HA gene belonged to clade 2.3.4.4B, while the NA gene had a close phylogenetic relationship with the 2.3.4.4C H5N6 highly pathogenic avian influenza viruses (HPAIVs) isolated from shoveler ducks in Ningxia in 2015. The NP gene was grouped into an independent subcluster within the 2.3.4.4B H5N8 AIVs, and the remaining six genes all had close phylogenetic relationships with the 2.3.4.4B H5N8 HPAIVs isolated from the wild birds in China, Egypt, Uganda, Cameroon, and India in 2016-2017, Multiple basic amino acid residues associated with HPAIVs were located adjacent to the cleavage site of the HA protein. The nine isolates comprised reassortant 2.3.4.4B HPAIVs originating from 2.3.4.4B H5N8 and 2.3.4.4C H5N6 viruses in wild birds. Conclusions: These results suggest that the Northern Tianshan Mountain wetlands in Xinjiang may have a key role in AIVs disseminating from Central China to the Eurasian continent and East African.

Development of Buried Type TDR Module for Leak Detection from Buried Pipe (매설관 주변부 누수 탐지를 위한 매설형 TDR 모듈 개발)

  • Hong, Wontaek
    • Journal of the Korean GEO-environmental Society
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    • v.22 no.11
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    • pp.31-37
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    • 2021
  • To prevent accidents due to the cavities and loosened layers formed due to water leakage from the deteriorated buried pipes, evaluation of the changes in water contents around the buried pipes is required. As a method to evaluate the water contents of the soils, time domain reflectometry (TDR) system can be adopted. However, slender electrodes used in standard TDR probe may be damaged when buried in the ground. Thus, in this study, buried type TDR module was developed for the evaluation of the water contents with maintaining required shape of the electrodes in the ground. The TDR module is composed of three electrodes connected to the core conductor and outer conductor and a casing to prevent deformation and maintain alignment of the electrodes in the ground. For the verification of TDR waveforms measured using the TDR module, comparative analysis was conducted with the TDR waveforms measured using the standard TDR probe, and the relationship between the volumetric water content of the soils and the travel time of the guided electromagnetic wave was constructed. In addition, a model test was conducted to test the applicability of the buried type TDR module, and the experimental result shows that the TDR module clearly evaluates the changes in volumetric water contents due to the leakage from the modeled buried pipe. Therefore, the buried type TDR module may be effectively used for the health monitoring of the buried pipe and the evaluation of the water contents around the pipes buried in the urban pavements.

Practitioners' Needs Analysis to Improve the Youth Theft Prevention Program (청소년 절도비행 재범방지 프로그램 개선을 위한 실무자 요구조사 연구)

  • Choi, Jaegwang;Jang, Heamin;Lee, Yu-Kyeong;Song, Wonyoung
    • Journal of Convergence for Information Technology
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    • v.11 no.9
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    • pp.145-156
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    • 2021
  • This study is aimed to improve the youth theft prevention program with rationality and practicality, based on the needs of practitioners who conduct these programs in fields. The survey questionnaire was prepared based on previous researches, and the survey was conducted on 72 probation officers and education instructors nationwide running a youth theft prevention program. As a result, practitioners reported the lack of self-control and high impulsivity as the main characteristics of the theft delinquent adolescents. And they recognized peer alignment and family problems as the causes of theft. As for the contents of the program, they reported that recidivism prevention skills such as understanding psychological problems, peer relationships, raising a sense of law compliance, anger, impulse control, dealing with negative emotions, and finding alternative behaviors, as well as moral development, are necessary. These results indicate that multi-dimensional interventions such as individuals, families, and peers are needed to prevent recidivism of juvenile theft. Implications of these results, future research directions, and limitations and significance of the study were discussed.

Development of Fiber-end-cap Fabrication Equipment (대구경 광섬유 엔드캡 제작장비 개발)

  • Lee, Sung Hun;Hwang, Soon Hwi;Kim, Tae Kyun;Yang, Whan Seok;Yoon, Yeong Gap;Kim, Seon Ju
    • Korean Journal of Optics and Photonics
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    • v.32 no.2
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    • pp.49-54
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    • 2021
  • In this paper, we design and construct the equipment to manufacture large-diameter optical fiber end caps, which are the core parts of high-power fiber lasers, and we fabricate large-diameter optical fiber end caps using the home-made equipment. This equipment consists of a CO2 laser as a fusion-splice heat source, a precision stage assembly for transferring the position of a large-diameter optical fiber and an end cap, and a vision system used for alignment when the fusion splice is interlocked with the stage assembly. The output of the laser source is interlocked with the stage assembly to control the output, and the equipment is manufactured to align the polarization axis of the large-diameter polarization-maintaining optical fiber with the vision system. Optical fiber end caps were manufactured by laser fusion splicing of a large-diameter polarization-maintaining optical fiber with a clad diameter of 400 ㎛ and an end cap of 10×5×2 ㎣ (W×D×H) using home-made equipment. Signal-light insertion loss, polarization extinction ratio, and beam quality M2 of the fabricated large-diameter optical fiber end caps were measured to be 0.6%, 16.7 dB, and 1.21, respectively.

Development of Selectable Marker of High Oleate Trait in Peanut (Arachis hypogaea L.) (땅콩에서 고 올레인산 형질관련 분자마커의 선발)

  • Yang, Kiwoung;Pae, Suk-Bok;Park, Chang-Hwan;Lee, Myoung Hee;Jung, Chan-Sik;Son, Jeong-Hee;Park, Keum-Yong
    • Korean Journal of Breeding Science
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    • v.42 no.5
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    • pp.507-514
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    • 2010
  • Peanut(Arachis hypogaea L.) is one of the major oilseed crops. The peanut oil consists of palmitic, oleic and linoleic acids, which are present at levels of 10%, 36-67% and 15-43%, respectively. High oleate mutant of peanut F435 contains 80% oleate and as little as 2% linoleate in seed oil. Previous study indicated that delta 12 fatty acid desaturase is a major enzyme controlling the oleate content in seeds of oilseed crops. F435 sequence alignment of their coding regions disclosed that an extra A(adenine) was inserted at the position +2,823 bp of delta 12 fatty acid desaturase gene. This study was to develop molecular marker (SNP marker) co-segregating with the high oleate trait. Chopyeong ${\times}$ F435 $F_2$ 41 population were investigated using molecular marker and fatty acid assay (NIR and gas chromatography). Finally, this marker segregates Chopyeong type 26 lines, heterotype 9 lines and F435 type 6 lines. These results in our study suggested that SNP marker conform fatty acid assay.

Radiographic Study of Cobey Method and Modified Cobey Method (Cobey 검사법과 Modified Cobey 검사법에 대한 방사선학적 연구)

  • Go, Yu-Rim;Joo, Young-Cheol;Lee, Seung-Keun
    • Journal of radiological science and technology
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    • v.42 no.3
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    • pp.167-173
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    • 2019
  • The Cobey method and the modified Cobey method are most commonly used in clinical practice. Therefore, the purpose of this study was to investigate the radiological differences between Cobey and modified Cobey and provide radiographic information about changes of hindfoot image with X-ray entrance center and tube angle change in modified Cobey. This study was performed on foot and ankle phantom. First, for image comparison of Cobey and modified Cobey, the images obtained by applying the same X-ray entrance center to the ankle joint were compared and analyzed. Second, in the modified Cobey, the X-ray entrance center is set as ankle joint and lateral malleolus. The X-ray tube angle was varied from $10^{\circ}$ to $40^{\circ}$ at $5^{\circ}$ intervals for each X-ray entrance center. The images obtained by varying the X-ray tube angle from $10^{\circ}$ to $40^{\circ}$ at intervals of $5^{\circ}$ for each X-ray entrance center were compared and analyzed. The irradiation conditions were the same with 110 kVp, 200 mA, 10 ms, and 110 cm of source - image receptor distance (SID). Image evaluation was performed by two radiologists. Measurements were made on the lateral point, middle point, and calcaneus width based on a hypothetical line parallel to the calcaneal tuberosity. Data were analyzed by using descriptive statistics as the mean of the distance to each measurement location. The modified Cobey was longer than the Cobey by an average of 3 to 4 mm lateral and medial points, and the calcaneus width was similar (ICC = 0.939). In modified Cobey method, when the X-ray entrance center is ankle joint, the lateral point is about 3 mm and the medial point is about 4.3 mm longer than lateral malleolus. Also, when the X-ray tube angle is more than $20^{\circ}$, the degree of distortion is large. The ICCs for the lateral, medial point, and calcaneus width were 0.998, 0.961, and 0.997, respectively, as the X-ray entrance center and tube angle were changed. There was no significant difference between Modified Cobey and Cobey. Modified Cobey showed no need to compensate the $20^{\circ}$ detector angle of the Cobey. In addition, we suggest that tube angle should be limited within $20^{\circ}$ when modified Cobey is performed.

Analysis of Spatial Trip Regularity using Trajectory Data in Urban Areas (도시부 경로자료를 이용한 통행의 공간적 규칙성 분석)

  • Lee, Su jin;Jang, Ki tae
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.17 no.6
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    • pp.96-110
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    • 2018
  • As the development of ICT has made it easier to collect various traffic information, research on creating new traffic attributes is drawing attention. Estimation and forecasts of demand and traffic volume are one of the main indicators that are essential to traffic operation, assuming that the traffic pattern at a particular node or link is repeated. Traditionally, a survey method was used to demonstrate this similarity on trip behavior. However, the method was limited to achieving high accuracy with high costs and responses that relied on the respondents' memory. Recently, as traffic data has become easier to gather through ETC system, smart card, studies are performed to identify the regularity of trip in various ways. In, this study, route-level trip data collected in Daegu metropolitan city were analyzed to confirm that individual traveler forms a spatially similar trip chain over several days. For this purpose, we newly define the concept of spatial trip regularity and assess the spatial difference between daily trip chains using the sequence alignment algorithm, Dynamic Time Warping. In addition, we will discuss the applications as the indicators of fixed traffic demand and transportation services.

A Method for Determining Face Recognition Suitability of Face Image (얼굴영상의 얼굴인식 적합성 판정 방법)

  • Lee, Seung Ho
    • Journal of the Korea Academia-Industrial cooperation Society
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    • v.19 no.11
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    • pp.295-302
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    • 2018
  • Face recognition (FR) has been widely used in various applications, such as smart surveillance systems, immigration control in airports, user authentication in smart devices, and so on. FR in well-controlled conditions has been extensively studied and is relatively mature. However, in unconstrained conditions, FR performance could degrade due to undesired characteristics of the input face image (such as irregular facial pose variations). To overcome this problem, this paper proposes a new method for determining if an input image is suitable for FR. In the proposed method, for an input face image, reconstruction error is computed by using a predefined set of reference face images. Then, suitability can be determined by comparing the reconstruction error with a threshold value. In order to reduce the effect of illumination changes on the determination of suitability, a preprocessing algorithm is applied to the input and reference face images before the reconstruction. Experimental results show that the proposed method is able to accurately discriminate non-frontal and/or incorrectly aligned face images from correctly aligned frontal face images. In addition, only 3 ms is required to process a face image of $64{\times}64$ pixels, which further demonstrates the efficiency of the proposed method.

RNCC-based Fine Co-registration of Multi-temporal RapidEye Satellite Imagery (RNCC 기반 다시기 RapidEye 위성영상의 정밀 상호좌표등록)

  • Han, Youkyung;Oh, Jae Hong
    • Journal of the Korean Society of Surveying, Geodesy, Photogrammetry and Cartography
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    • v.36 no.6
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    • pp.581-588
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    • 2018
  • The aim of this study is to propose a fine co-registration approach for multi-temporal satellite images acquired from RapidEye, which has an advantage of availability for time-series analysis. To this end, we generate multitemporal ortho-rectified images using RPCs (Rational Polynomial Coefficients) provided with RapidEye images and then perform fine co-registration between the ortho-rectified images. A DEM (Digital Elevation Model) extracted from the digital map was used to generate the ortho-rectified images, and the RNCC (Registration Noise Cross Correlation) was applied to conduct the fine co-registration. Experiments were carried out using 4 RapidEye 1B images obtained from May 2015 to November 2016 over the Yeonggwang area. All 5 bands (blue, green, red, red edge, and near-infrared) that RapidEye provided were used to carry out the fine co-registration to show their possibility of being applicable for the co-registration. Experimental results showed that all the bands of RapidEye images could be co-registered with each other and the geometric alignment between images was qualitatively/quantitatively improved. Especially, it was confirmed that stable registration results were obtained by using the red and red edge bands, irrespective of the seasonal differences in the image acquisition.