• Proceedings of the Zoological Society Korea Conference
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• 1995.10b
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• pp.311.1-311
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• 1995

• Journal of The Korean Society of Grassland and Forage Science
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• v.29 no.3
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• pp.165-170
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• 2009

A system for the production of transgenic plants has been developed for perennial ryegrass (Lolium perenne L.) via Agrobacterium-mediated transformation. Included in this study were two factors which may affect the gene transfer efficiency: concentrations of acetosyringone (AS, 0 to 300 ${\mu}M$), and co-culture period (1 to 7 days). Both factors were very important to achieve high efficiency gene transformation in the perennial ryegrass. The highest transformation efficiency was obtained when embryogenic calli were inoculated with Agrobacterium in the presence of 100 ${\mu}M$ AS with the culture medium for 5 days. Phosphinothricin resistant calli were developed with into complete plants. GUS histochemical assay, polymerase chain reaction (PCR) and Northern blot analysis of transgenic plants demonstrated that transgenes were integrated into the genome of perennial ryegrass. Using this protocol, it was possible to obtain transformants efficiently for further study.

• Journal of Plant Biotechnology
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• v.33 no.1
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• pp.49-56
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• 2006

Gerber (Gerbera hybrida) is a valuable ornamental species grown as a potted plant and cut flowers. However, genetic variability within the gerbera genus is very limited. So it is absolutely needed to introduce and widen genetic resources into gerbera lines by genetic transformation. For the purpose, 18 Korean gerbera lines were screened to establish Agrobacterium-mediated genetic transformation procedure. In an experiment to select Korean gerbera lines which are amenable to Agrobacterium-inoculation, 12 lines turned out to be positive in Agrobacterium-inoculation. More callus were produced from BA 2ppm, Zeatin 2ppm, IAA 0.2ppm in pre-culture and regeneration medium (2X media) but there was no difference in the frequency of GUS expression rate. In another experiment to find out optimal condition for highly efficient Agrobacterium-inoculation, petiole and leaf explants have been treated with four different pre-culture periods, two different co-culture periods and two different Agrobacterium strains. As a result, high GUS expression has been showed from petiole and leaf explants treated no pre-culture period with LBA4404 Agrobacterium tumerfaciens, 5 day co-culture period and dipping treatment.

• Journal of Plant Biotechnology
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• v.35 no.2
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• pp.109-114
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• 2008

The aim of this research is to establish the conditions for Agrobacterium-mediated genetic transformation using microspore. The embryo induction from the microspore was examined under several Kanamycin concentration in media, and the induction rate decreased about 4, 8, 10 times when the Kanamycin concentration increased 10, 50, 100 mg/L, respectively. This indicates that the transformation rate would be much lower if the Kanamycin was used for selection marker. In order to apply the GFP gene as a reporter gene for Agrobacterium-mediated genetic transformation, GFP expression from the microspore-mediated embryos was observed using GFP filter under microscope. The GFP expression occurred when the microspore cultured toward the embryo development for 12, 24 and 48 days. The microspore formed a cluster by microspore division from 12 days culture and continuously became a bigger mass. We obtained a total of 8 GFP-expressing embryos suggesting that the transformation of microspore occurred. However, those young embryos were not fully developed. Further study pertinent to culture conditions is required to fulfill the Agrobacterium-mediated genetic transformation using microspore.

• The Microorganisms and Industry
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• v.12 no.2
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• pp.2-13
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• 1986

Agrobacterium tumefaciens의 연구는 유전공학 시대를 맞이하여 많은 연구자들의 커다란 주목을 끌고있다. 식물세포내에 외부 유전자를 도입시키는, 확실히 믿을 수 있는 vector로 등장된 때문이다. 원래 이세균은 식물 줄기나 뿌리에 암종을 유발시키므로서 암발성 원인 구명 연구로 흥미를 끌게 되었다. 연구결과는 암발생 예방및 치료에 목적을 둠은 덩연할 것이다. 많은 약제가 시험되었으나 별로 진전을 보지 못하던중 비 원인성인 Agrobacterium radiobacter, strain 84에 의한 생물학적 방제의 성공으로 유일한 방제법을 갖게되었다. 뒤이어 암종발생 기작도 밝혀졌다. Agrobacterium의 세계는 온통 유전공학 기술로 채워져 있다. 암종발생에서 방제원리에 이르기까지 수없이 먼 옛날부터 이미 익혀오던 DNA 조작기술이었던가\ulcorner 암종을 유발시키는 agrocin84 plasmid를 갖는 비병원성 Agrobacterium을 찾아 생물학적 방제법을 확립하였다. 그후 병원성 Agrobacterium은 이에 대하여 어떻게 살아남을 것인가\ulcorner 실로 놀라운 일이라 아니할 수 있을까\ulcorner 이 병원성 Agrobacterium은 비 병원성 Agrobacterium 속에 있는 agrocin 84 plasmid을 탈취하여 자신이 agrocin84를 생성분비하며 암종 유발을 계소하여 간다. 아니면 비병원성 Agrobacterium이 병원성 Agrobacterium에게 agrocin 84 plasmid를 넘겨주었을까\ulcorner 왜 넘겨주었을까\ulcorner 공존을 위하여서일까\ulcorner 우리의 유전공학 기술은 이것을 막아줄수 있을까\ulcorner 생물학적 방제의 재성공을 위하여 논제의 연구는 왜 필요했던가\ulcorner 그 전후를 여기에 서술해 본다.닭이며 또한 제한된 지면에서 충분히 고찰하기는 어렵다. 우리나라에서 자주 거론되는 백신 및 종류에 국한하여 그 문제점과 앞으로의 전망을 고찰해 보기로 한다.ocking electrode를 제작하여 복합고분자 전해질과의 계면저항을 측정하였다.nm (1.2921eV)는 acceptor-bound exciton 인 I1(AO,X) 이고, 964.6nm(1.2853eV)는 donor-acceptor pair(DAP) 발광, 1341.9nm (0.9239eV)는 self activated(SA)에 기인하는 광발광 봉우리로 고찰되었다.가 높을수록 방출전류가 시간에 따라 급격히 감소하였다. 각 duty비에서 방출전류의 양이 1/2로 감소하는 시점을 에미터의 수명으로 볼 때 duty비 대 에미터 수명관계를 구해 높은 duty비에서 전계방출을 시킴으로써 실제의 구동조건인 낮은 duty비에서의 수명을 단시간에 예측할 수 있었다. 단속적으로 일어난 것으로 생각된다.리 폐 관류는 정맥주입 방법에 비해 고농도의 cisplatin 투여로 인한 다른 장기에서의 농도 증가 없이 폐 조직에 약 50배 정도의 고농도 cisplatin을 투여할 수 있었으며, 또한 분리 폐 관류 시 cisplatin에 의한 직접적 폐 독성은 발견되지 않았다이 낮았으나 통계학적 의의는 없었다[10.0%(4/40) : 8.2%(20/244), p>0.05]. 결론: 비디오흉강경술에서 재발을 낮추기 위해 수술시 폐야 전체를 관찰하여 존재하는 폐기포를 놓치지 않는 것이 중요하며, 폐기포를 확인하지 못한 경우와 이차성 자연기흉에 대해서는 흉막유착술에 더 세심한 주의가 필요하다는 것을 확인하였다. 비디오흉강경수술은 통증이 적고, 입원기간이 짧고, 사회로의 복귀가 빠르며, 고위험군에 적용할 수 있고, 무엇보다도 미용상의 이점이 크다는 면에서 자연기흉에 대해 유용한 치료방법임에는

• Journal of Korean Society of Forest Science
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• v.77 no.2
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• pp.199-207
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• 1988

Three clones of Populus alba ${\times}$ P. grandidentata have been tested for susceptibility to Agrobacterium tumefaciens strains A281 and A348. We determined the optimum concentration of kanamycin sulfate for effective selection of leaf disc-derived, transgenic tissues transformed using Agrobacterium binary vector pGA472 containing a neomycin phosphotransferase gene (NPT-II) which confers kanamycin resistance. Of the wild type Ti plasmids contained by the two Agrobacterium strains, pTiBo542 of strain A281 appears to be best suited to serve as a helper plasmid for binary vector systems. A relatively low concentration (10mg/l) of kanamycin sulfate inhibited adventitious shoot initiation from leaf discs on regeneration medium. Transformed kanamycin-resistant calli were obtained by culturing Agrobacterium inoculated leaf discs on selective regeneration medium. The transformed kanamycin-resistant calli continued to grow on regeneration media supplemented with kanamycin sulfate to levels of 50 and 200mg/l. The growth of non-co-cultivated control calli was severely inhibited on regeneration medium containing 50mg/l kanamycin sulfate.

• Journal of Plant Biotechnology
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• v.50
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• pp.63-69
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• 2023

This study includes the transformation of genes such as ORE7, the increase of gene expression, and the use of the bar gene as a selectable marker that shows herbicide resistance with Agrobacterium tumefaciens using hypocotyls from the oilseed rape "Youngsan" cultivar. To establish an Agrobacterium transformation system for the production of oilseed rape with a high-yield trait, infection time and co-cultivation period with Agrobacterium were tested. Therefore, when hypocotyls from the oilseed rape "Youngsan" cultivar were infected with Agrobacterium for 20 min and co-cultivated for 3 days, approximately 32-36 putatively transformed hypocotyls with shoots including roots survived from 100 inoculated hypocotyls after 4 weeks of transformation on a selection medium containing 20 mg/L of phosphinothricin (PPT) as a selectable agent. Additionally, a PCR assay was performed to confirm the insertion of target genes and showed the presence of the ORE7 gene as a high-yielding trait and the bar gene as a selectable marker. Treatment with 0.5% (v/v) Basta solution as a selectable agent for 6 days with leaves from transformed oilseed rape expressed the bar gene. Therefore, this study can contribute to the development of special oilseed rapes containing agriculturally useful traits such as herbicide resistance, drought tolerance, high yielding traits, and high oleic acid content.

• Korean Journal of Agricultural Science
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• v.38 no.2
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• pp.213-217
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• 2011

Agrobacterium rhizogenes, a gram-negative soil bacterium, is one of the most widely studied among them. A, rhizogenes can transfer T-DNA, excised from Ri (root inducing)-plasmids from the bacterial to the plant cell. It is the causal agent of 'hairy root' diseases in plants, and has been used for the production of hairy root cultures from a multitude of species. Five different strains of Agrobacterium rhizogenes differed in their ability to induce Scutellaria baicalensis hairy roots and also showed varying effects on the growth in hairy root cultures. A. rhizogenes R 1000 is the most effective strain for the induction (57.3%) and growth (11.9 g $L^{-1}$) in hairy root of Scutellaria baicalensis. Our results demonstrate that use of suitable strains of A. rhizogenes may allow study of the regulation of flavone biosynthesis in hairy root cultures of Scutellaria baicalensis.

• Journal of the Korean Society of Tobacco Science
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• v.13 no.1
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• pp.43-52
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• 1991

These studies were carred out to obtain the transformant from tobacco cells by Agrobacterium spp. from crown gall and soil at the natural field in Korea, and identified their virulence. Kodo's and Clark's selective media were used for isolation of Agrobacterium spp. In these media, total of 99 strains were characterized based on the morphological characteristics of colonies. Among them 34 strains were able to induce on carrot discs. And hypervirulent strains C23-1 and K29-1 were identified as Agrobacterium tumefaciens biotype 1 and biotype 2, respectively. These strains formed fast growing, larger gall as compared to those induced by other strains on the carrot discs. Transformed tobacco callus was initiated on the phytohormone free MS medium with 250$\mu\textrm{g}$/ml carbenicillin after co-cultivation of tobacco stem explants and Agrobacteria. On the phytohormone free media, shoot was rarely formed from transformed callus. However, these shoot were teratoma shoots which were not grown as normal shoot, and teratoma shoot from transformant by C23-1 was smaller than that of K29-1.

• Proceedings of the Plant Resources Society of Korea Conference
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• 2018.10a
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• pp.52-52
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• 2018

Development of herbicide resistant transgenic legume plants through Agrobacterium-mediated transformation has been worked in many previous studied. Plant regeneration after infection is the important step to obtain successful transgenic plants. Many attempts try to find the optimum media condition for plant regeneration after infection. However, the transformation efficiency of legume plants is still low. In this study, regeneration of some Korean legume species including two soybean cultivars (Dawon and Pungsan) and pea have been done with organogenesis which is used various kind of explants such as cotyledonary-nodes in soybean and bud-containing tissue in pea. We developed the optimum media condition for plant regeneration regulators under Agrobacterium-mediated transformation using different kind and various concentration of plant growth. As the results, B5 medium containing 2 mg/L of 6-benzylaminopurine was selected in this study for the optimum plant regeneration media. The segments were inoculated with Agrobacterium suspension harbored an IG2 vector containing bar gene which confers resistance to phosphinotricin (PPT) in 3, 5 and 7 days. The transformation efficiency was achieved in Dawon 3.03 % and pea 1.46 % with co-cultivation period of 7 days which is showed a high number of GUS positive expression period.