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http://dx.doi.org/10.7744/cnujas.2011.38.2.213

Influence of different strains of Agrobacterium rhizogenes on hairy root induction and growth in Scutellaria baicalensis  

Park, Woo-Tae (Department of Crop Science, Chungnam National University)
Kim, Young-Seon (Department of Crop Science, Chungnam National University)
Park, Nam-Il (Department of Crop Science, Chungnam National University)
Kim, Haeng-Hoon (National Agrobiodiversity Center, National Academy of Agricultural Science, Rural Development Administration (RDA))
Lee, Sook-Young (Chosum University Dental Hospital)
Park, Sang-Un (Department of Crop Science, Chungnam National University)
Publication Information
Korean Journal of Agricultural Science / v.38, no.2, 2011 , pp. 213-217 More about this Journal
Abstract
Agrobacterium rhizogenes, a gram-negative soil bacterium, is one of the most widely studied among them. A, rhizogenes can transfer T-DNA, excised from Ri (root inducing)-plasmids from the bacterial to the plant cell. It is the causal agent of 'hairy root' diseases in plants, and has been used for the production of hairy root cultures from a multitude of species. Five different strains of Agrobacterium rhizogenes differed in their ability to induce Scutellaria baicalensis hairy roots and also showed varying effects on the growth in hairy root cultures. A. rhizogenes R 1000 is the most effective strain for the induction (57.3%) and growth (11.9 g $L^{-1}$) in hairy root of Scutellaria baicalensis. Our results demonstrate that use of suitable strains of A. rhizogenes may allow study of the regulation of flavone biosynthesis in hairy root cultures of Scutellaria baicalensis.
Keywords
Agrobacterium rhizogenes; Hairy root; Scutellaria baicalensis; Strains;
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