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http://dx.doi.org/10.5010/JPB.2006.33.1.049

Optimization of Genetic Transformation Conditions for Korean Gerbera Lines  

Lee, Hye-Young (Dept. of Plant Genetic Engineering, Dong-A University)
Lee, Ki-Jung (Dept. of Plant Genetic Engineering, Dong-A University)
Jeon, Eun-Hee (Dept. of Plant Genetic Engineering, Dong-A University)
Shin, Sang-Hyun (Dept. of Plant Genetic Engineering, Dong-A University)
Lee, Jai-Heon (Dept. of Plant Genetic Engineering, Dong-A University)
Kim, Doh-Hoon (Dept. of Plant Genetic Engineering, Dong-A University)
Chung, Dae-Soo (Dept. of Plant Genetic Engineering, Dong-A University)
Chung, Yong-Mo (Flower Breeding Research Institute, Gyeongnam ARES)
Cho, Yong-Cho (Flower Breeding Research Institute, Gyeongnam ARES)
Kim, Jeong-Kook (School of Life Science and Biotechnology, Korea University)
Chung, Young-Soo (Dept. of Plant Genetic Engineering, Dong-A University)
Publication Information
Journal of Plant Biotechnology / v.33, no.1, 2006 , pp. 49-56 More about this Journal
Abstract
Gerber (Gerbera hybrida) is a valuable ornamental species grown as a potted plant and cut flowers. However, genetic variability within the gerbera genus is very limited. So it is absolutely needed to introduce and widen genetic resources into gerbera lines by genetic transformation. For the purpose, 18 Korean gerbera lines were screened to establish Agrobacterium-mediated genetic transformation procedure. In an experiment to select Korean gerbera lines which are amenable to Agrobacterium-inoculation, 12 lines turned out to be positive in Agrobacterium-inoculation. More callus were produced from BA 2ppm, Zeatin 2ppm, IAA 0.2ppm in pre-culture and regeneration medium (2X media) but there was no difference in the frequency of GUS expression rate. In another experiment to find out optimal condition for highly efficient Agrobacterium-inoculation, petiole and leaf explants have been treated with four different pre-culture periods, two different co-culture periods and two different Agrobacterium strains. As a result, high GUS expression has been showed from petiole and leaf explants treated no pre-culture period with LBA4404 Agrobacterium tumerfaciens, 5 day co-culture period and dipping treatment.
Keywords
Gerbera hybrida; Agrobacterium tumerfaciens; cefotaxime; Zeatin; IAA; BA (6-Benzlaminopurine); GUS ($\beta$-glucuronidase);
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