• Korean journal of applied entomology
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• v.58 no.4
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• pp.363-380
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• 2019

Previous studies on the host plant range of Metcalfa pruinosa were conducted without distinguishing between its stages of development. In this study, we investigated host plants by studying the nymph and adult development stages of M. pruinosa. M. pruinosa nymphs were found on host plants that belong to 78 families and 227 species, and, to the best of our knowledge, host plants that belong to 27 families and 38 species have been reported for the first time. The host plants were divided into woody and herbaceous at the nymph stage of M. pruinosa, and the nymphs were found in 110 herbaceous and 117 woody species. M. pruinosa adults were found on host plants that belong to 87 families and 233 species, and, host plants that belong to 26 families and 36 species have been reported for the first time. The host plants were divided into woody and herbaceous at the adult stage of M. pruinosa, and the adults were found in 105 herbaceous and 128 woody species. Therefore, the total domestic host plant of M. pruinosa was 98 families 345 species. The nymph and adult in preoviposition stage prefer Helianthus annuus and the adult in oviposition stage prefer Persicaria tinctoria and Rosa rugosa.

• Proceedings of the National Institute of Ecology of the Republic of Korea
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• v.3 no.1
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• pp.7-12
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• 2022

Farming practices that balance environmental friendliness with biodiversity are increasingly valuable. Wild plants on farmlands compete for nutrients with crops and create a crucial microhabitat and resources for animals such as natural enemies. Investigating farmlands and their surrounding plants with limited human and material resources has become an essential aspect of evaluating the agricultural ecosystem services. This study investigated plants in six agricultural long-term ecological research sites in Taiwan from 2017 to 2020 to determine the ideal season for investigation. Cluster analysis was performed to group habitats with similar plant composition, and the species-area curves of the clusters in each season were created. The results indicated that the agricultural ecosystem could be divided into farmlands, banks, orchards, and tea gardens. The habitats were divided into farmland, bank, Chia-Yi orchard, Gu-Keng orchard, and tea garden clusters. Ground plant cover can be investigated all year with at least 18 quadrats. However, if human and material resources are limited, 10 quadrats should be the minimum for farmlands in autumn and for the other microhabitats in spring. The minimum number of quadrats is 10 for banks, 17 for orchards, and 9 for tea gardens.

• The Plant Pathology Journal
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• v.39 no.4
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• pp.384-396
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• 2023

Colletotrichum acutatum species complex is one of the most important groups in the genus Colletotrichum with a high species diversity and a wide range of host plants. C. acutatum and related species have been collected from different plants and locations in Korea and deposited into the Korean Agricultural Culture Collection (KACC), National Institute of Agricultural Sciences since the 1990s. These fungal isolates were previously identified based mainly on morphological characteristics, and a limitation of molecular data was provided. To confirm the identification of species, 64 C. acutatum species complex isolates in KACC were used in this study for DNA sequence analyses of six loci: nuclear ribosomal internal transcribed spacers (ITS), betatubulin 2 (TUB2), histone-3 (HIS3), glyceraldehyde3-phosphate dehydrogenase (GAPDH), chitin synthase 1 (CHS-1), and actin (ACT). The molecular analysis revealed that they were identified in six different species of C. fioriniae (24 isolates), C. nymphaeae (21 isolates), C. scovillei (12 isolates), C. chrysanthemi (three isolates), C. lupini (two isolates), and C. godetiae (one isolate), and a novel species candidate. We compared the hosts of KACC isolates with "The List of Plant Diseases in Korea", previous reports in Korea and global reports and found that 23 combinations between hosts and pathogens could be newly reported in Korea after pathogenicity tests, and 12 of these have not been recorded in the world.

• Korean Journal of Ecology and Environment
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• v.50 no.2
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• pp.254-265
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• 2017

To elucidate occurrence patterns of naturalized plants in agroecosystems, we surveyed flora in uplands, orchards and rice paddy fields in middle regions (Gyeonggi-do and Chungcheong-do) of Korea in summer and fall from 2013 to 2015. Our results showed that there are 77 species of naturalize plants in 54 genera and 20 families of vascular plants among total 420 species occurred in the agricultural areas composing 18.3% of naturalized index. Community compositions of both native and naturalized plants were different among agricultural types. We suggested that Cerastium glomeratum Thuill., Bidens frondosa L. and Erigeron philadelphicus L. are capable of dispersal to other regions based on our results with getting to broaden distribution of naturalized plants. The mean naturalized index was the highest in the upland (25.1%) followed by in the orchard (23.0%) and the paddy field (16.1%), while the mean number of naturalized species was the highest in the orchard (21.8 species) followed by in the upland (16.6 species) and paddy field (10.3 species). The numbers of naturalized plants in the orchard were increased along with increasing size of the agricultural land and decreasing ecological area rate. In addition, the number of native species was significantly correlated with the number of naturalized species in the upland. Our results would provide useful information to manage dispersion of naturalized plants in agricultural ecosystems.

• Korean Journal of Environmental Biology
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• v.39 no.1
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• pp.1-5
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• 2021

Radish is one of the major vegetable crops and has been damaged economically by the causal agent of Fusarium wilt disease, Fusarium oxysporum f. sp. raphani (FO) in the highlands of Gangwon province, Korea. Recently, clover cyst nematode, Heterodera trifolii(HT), occurred in highland of Jeongseon area and poses a threat to radish plants. The nematodes, besides causing direct damage to radish plants, possess the capability to synergize with other plant pathogens, leading to the development of a disease complex. The aim of this study was to investigate the interaction between FO and HT in radish plants. The length of plants co-infected with both FO and HT was shorter than that of plants infected with FO alone, but there was no statistically significant difference. However, the disease severity was much higher in the plants co-infected with both FO and HT(1.60±0.97) compared to FO alone(0.20±0.42)(df=3, F=10.17, p<0.001). Disease incidence was also about two-fold higher in plants co-infected with both FO and HT (80%) compared to FO alone (44%). These results suggested that the clover cyst nematode should be considered in the management of Fusarium wilt disease in the highlands of Gangwon province.

• Journal of Microbiology and Biotechnology
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• v.26 no.1
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• pp.160-170
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• 2016

The increasing spread of antibiotic-resistant pathogens has raised the interest in alternative antimicrobial treatments. In our study, the functionally active gram-negative bacterium bacteriophage CP933 endolysin was produced in Nicotiana benthamiana plants by a combination of transient expression and vacuole targeting strategies, and its antimicrobial activity was investigated. Expression of the cp933 gene in E. coli led to growth inhibition and lysis of the host cells or production of trace amounts of CP933. Cytoplasmic expression of the cp933 gene in plants using Potato virus X-based transient expression vectors (pP2C2S and pGR107) resulted in death of the apical portion of experimental plants. To protect plants against the toxic effects of the CP933 protein, the cp933 coding region was fused at its Nterminus to an N-terminal signal peptide from the potato proteinase inhibitor I to direct CP933 to the delta-type vacuoles. Plants producing the CP933 fusion protein did not exhibit the severe toxic effects seen with the unfused protein and the level of expression was 0.16 mg/g of plant tissue. Antimicrobial assays revealed that, in contrast to gram-negative bacterium E. coli (BL21(DE3)), the gram-positive plant pathogenic bacterium Clavibacter michiganensis was more susceptible to the plant-produced CP933, showing 18% growth inhibition. The results of our experiments demonstrate that the combination of transient expression and protein targeting to the delta vacuoles is a promising approach to produce functionally active proteins that exhibit toxicity when expressed in plant cells.

• Applied Biological Chemistry
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• v.37 no.4
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• pp.248-254
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• 1994

Bromoxynil is an antidicot herbicide widely used on cereal crops and has a short half life in the soil. A bxn gene, encoding a specific nitrilase that converts bromoxynil to its primary metabolite 3,5-dibromo-4-hydroxybenzoic acid, was inserted in plant binary vector pGA482, and then introduced into tobacco and lettuce plants via Agrobacterium mediated leaf-disc transformation method. Transgenic plants with the bxn gene were selected by kanamycin and regenerated to whole plants. The regenerated transgenic plants were determined level of expression of bxn gene by Northern blot analysis. Leaf-disc analysis and pot-assay confirmed that the transgenic tobacco and lettuce plants were resistant to high doses of bromoxynil.

• Korean Journal of Plant Tissue Culture
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• v.26 no.1
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• pp.65-69
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• 1999

Effect of chemo- and thermotherapy on LSV elimination was investigated in Lilium Oriental Hybrid cv. Casa Blanca which was infected by LSV. Either 20 mg/L ribavirin or the treatment of 35$^{\circ}C$/$25^{\circ}C$ (day/night) thermotherapy resulted in the desired growth rate and high percentage (86%, 80% respectively) of LSV elimination. When we combinated those application, the rate of LSV elimination was increased with the time of heat treatment, and was 100% by all heat treatment of 16 weeks. After all of the LSV-free plants transferred into the soil, the number of LSV-free plants was 8 plants out of 12 plants and the efficiency of LSV elimination was best in the combination of 20 mg/L ribavirin and 8 weeks of heat treatment. About 21 % of plants kept LSV-free after transferred all of the LSV-free plants into the soil, the others became LSV-infected again.

• The Plant Pathology Journal
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• v.19 no.5
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• pp.253-256
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• 2003

Citrus canker disease is caused by bacteria Xanthomonas axonopodis .pv. Citri. Shiranuhi cultivar, a hybrid of Kiyomi tangor and Nakano No.3 ponkan was evaluated for resistance to citrus canker based on initiation of disease, percent area of lesion infected and growth rate of bacteria in the leaf under growth chamber condition. Significant differences between susceptible plant and resistant plants were observed in these assays. Resistant plants showed delayed disease symptoms compared to the susceptible plants after spray inoculation of the pathogen. The resistant verities, satsuma, yuzu, and Shiranuhi showed symptoms after six days where as susceptible, mexican lime showed the symptoms just after three days of inoculation. 18 days after inoculation, percent area of lesions developed on leaf and disease severity differed significantly in susceptible and resistant plants, and were ranked as follows: mexican lime > early satsuma =Shiranuhi =yuzu (P <, 0.01). However, 30 days after inoculation, percent area of lesion was further differentiated into resistant and highly resistant plants. That was ranked as follows: sweet orange> early satsuma =Shiranuhi =Kiyomi > yuzu (P < 0.01). These results indicate that host reaction to the bacterial was more distinct when the disease developed for a longer period. Growth rates of a citrus canker bacterium during 16 40 h also were distinct after infiltration into leaves of susceptible and resistant plants, and were ranked as follows: sweet orange> early satsuma =Shiranuhi =Kiyomi =yuzu (P < 0.01). Based on these results, we concluded that Shiranuhi is resistant to citrus canker as compared to Kiyomi, early satsuma, and yuzu.

• Journal of People, Plants, and Environment
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• v.23 no.6
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• pp.615-624
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• 2020

Background and objective: Urbanization caused a reduction in urban green space and a lack of community spaces. An attempt to solve these problems, urban gardens, have become popular and are currently being implemented in various downtown areas. However, urban gardens have some inadequate aspects from a visual or landscape perspective. The goal of this study was to examine the possibility of an aesthetic and productive garden space by introducing cultivation methods through the planting system design process. Methods: The design process using plants was classified and presented to suggest the importance of the landscape and aesthetic value. An urban garden space was designed according to the perspective of 'production scenery' and 'participation aesthetics'. According to the characteristics of the plant, urban gardens were divided into vertical type (corn, millet, sorghum), climbing type (kidney bean, cucumber, bitter gourd), and runner type (melon, watermelon, peanut). After classifying plants according to the shape of the root, the structure supporting climbing was installed and the crops were cultivated in an upright form with a tunnel. Results: In the designed cultivation, each crop cooperates, without invading each other's space. Compared with the conventional cultivation, there was little difference in production, and management was made more convenient since weed outbreaks were effectively suppressed while runner type crops cover over the land's surface. Since the positions of each crop are clearly distinguished, the aesthetic value is improved by offering a sense of rhythm with a balanced design. Conclusion: The results suggest that the cultivation methods through the plant system design process have aesthetic as well as productive value, and the design using plants, an infinite living resource, could lead to an expansion of the design field. Moreover, it would enable a sustainable symbiosis between industry and environment. There is potential for the design industry to make significant progress through collaboration with agriculture, horticulture, and landscape architecture.