• Korean journal of applied entomology
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• v.60 no.4
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• pp.357-361
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• 2021

Monitoring about nine high risk insect pests, Aceria diospyri, Bactrocera dorsalis, Bactrocera minax, Bactrocera tsuneonis, Cydia pomonella, Lobesia botrana, Proeulia sp., Solenopsis invicta and Stephanitis takeyai, were carried out in seven regions from April to October in 2020. A total of 12,045 traps/visual scouting were investigated in 222 points of 78 local sites of seven regions, resulting the nine species, A. diospyri, B. dorsalis, B. minax, B. tsuneonis, C. pomonella, L. botrana, Proeulia sp., S. invicta, and S. takeyai, were not detected. This study has been conducted from 2018 to 2020, and we established the nationwide monitoring system and secured a bridgehead for monitoring invasive insect pests passing the border including seven universities.

• International Journal of Industrial Entomology and Biomaterials
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• v.47 no.2
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• pp.126-133
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• 2023

Genetic resources of mulberry trees are commonly preserved as trophosomes, which are vulnerable to environmental factors, such as natural disasters, diseases, and pests. This study establishes a basic protocol for ultra-low temperature cryopreservation of mulberry trees using a two-step freezing process. The procedure was established using the "Daeshim" variety and then tested on genetic resources from 24 other mulberry varieties. Samples were first dried to a moisture content of 33-43% in a low-temperature forced-air chamber at -5 ℃, then slowly frozen from -5 ℃ to -20 ℃, and preserved in liquid nitrogen (-196 ℃). To determine the regeneration rate, isolated dormant buds were inoculated into MS basal medium, and grown shoots were grafted onto 1-year-old rootstock via chip budding and then cultured. After freezing in liquid nitrogen, the "Daeshim" variety exhibited a survival and regeneration rate of more than 70% and 50%, respectively. Applying the two-step freezing process to genetic resources from 24 mulberry species yielded average survival and regeneration rates of 85.3% and 75.5%, respectively. Morus alba showed survival and regeneration rates of 100%, confirming the efficacy of the two-step freezing method. These results indicate the high feasibility of ultra-low-temperature cryopreservation through two-step freezing of dormant buds from mulberry genetic resources. Additional research is required into the variations in regeneration rates with freezing period in liquid nitrogen.

• International Journal of Industrial Entomology and Biomaterials
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• v.8 no.1
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• pp.77-80
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• 2004

For the development of the alternative control system against the major forest pests, Beauveria spp. F-101, isolated from a dead larva of Thecodiplosis japonensis, was selected because this isolate showed high pathogenicities against T. japonensis and Acantholyda parki. Beauveria spp. F-101 had irregular clustered conidio-phores and conidia borne on a distinctive apical zigzag extension, and it showed typical characteristic of the genus, Beauveria in morphology. For molecular based-identification, the ribosomal ITS region of Beauveria spp. F-101 was amplified with ITS1 and ITS4 primers, and cloned into pGEM- T Easy vector. The amplified PCR product was 569 bp in size and completely sequenced. The similarities of the cloned ITS sequence were 99 ％ and 97％ to those of B. bassiana and B. brongniartii, respectively. In comparison to other species among the genus Beauveria, the ITS region of Beauveria spp. F-101 showed a similarity of 95％ to B. amorpha, 95％ to B. tenella, 89％ to B. vermiconia and 69％ to B. alba, respectively. In addition, in comparison to different genus, it had 95％ similarities to Cordyceps militaris and 91％ to Paecilomyces tenuipes. Accordingly, the current result suggests that Beauveria spp. F-101 was a variant of B. bassiana and it seems to be a new isolate considering sequence variation in ITS region.

• KOREAN JOURNAL OF CROP SCIENCE
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• v.52 no.2
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• pp.183-197
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• 2007

Field trial was conducted at the Research Farm of the Katsina State Agricultural and Rural Development Authority during the farming season of 2004 with a view to evaluate/determine the efficacy of some selected plant-derived biopesticides against the insect pests of cowpea as well as their effect on yield. The variety of the cowpea used was IT86D-719 and the plant derived biopesticides evaluated during the trial were chilli pepper, garlic, ginger, neem, sweetsop and tobacco. The experimental field was ploughed, harrowed and thereafter ridged before the commencement of the 2004 planting season. A total of twenty one (21) experimental plots were demarcated and arranged into seven treatment plots. The size of each plot was $5m{\times}4m$ while interspaces between adjacent plot and blocks were 1m and 2m, respectively. Results of the experiments showed that all the plant-derived biopesticide treatments were significantly (p>0.05) better than control treatment. The order of effectiveness of the treatments was tobacco (80-90%), sweetsop (75-85%), garlic (70-80), neem (72-78%), chilli pepper (60-70%) and ginger (30-50%). Furthermore, yield result corresponded positively with the effectiveness of the treatments. Results of the present finding suggest the use of tobacco, sweetsop and garlic as promising biopesticides in the control of cowpea insect pests.

• Proceedings of the Korean Society for Bio-Environment Control Conference
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• 2004.03a
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• pp.263-272
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• 2004

• Korean Journal of Organic Agriculture
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• v.19 no.spc
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• pp.263-266
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• 2011

Crop protection strategies in organic horticulture aim to prevent insect pest and plant disease problems through utilization of non-chemical based control means. In order to develop a model for management of plant diseases and insects in organic cucumber cultivation, we compared efficacies between chemical pesticide spraying system and biological control means in semi-forcing and retarding cucumber cultivation during 2005 and 2006. Conventional chemical spray program using various chemical pesticides was applied 5 - 10 days intervals, while two different non-chemical pesticide application programs using two formulated biopesticides Topseed$^{TM}$ and Q-fect$^{TM}$, Suncho$^{TM}$, and Sangsungje$^{TM}$ (biocontrol agents 1) and using egg-yolk and cooking oil(EYCO), Bordeaux mixture, Suncho$^{TM}$, and Sangsungje$^{TM}$ (biocontrol agents 2) were applied 5 - 7 days intervals during entire cucumber cultivation period. Efficacy of both biocontrol agents programs was effective to comparable to conventional chemical pesitice spray program to control plant diseases such as powdery mildew and downy mildew as well as insect pests such as aphids and thrips which are known as major threats in cucumber organic cultivation. In this study, we established and evaluated an effective and economic crop protection strategy using various biological resources can be used to control plant diseases and pests simultaneously in organic cucumber cultivation field.

• Korean Journal of Agricultural and Forest Meteorology
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• v.16 no.4
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• pp.368-383
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• 2014

Climate change is expected to affect population density, phenology, distribution, morphological traits, reproduction and genetics of insects, and even in the extinction of insects. To develop novel research subjects for predicting climate change effect, basic information about biological and ecological data on insect species should be compiled and reviewed. For this reason, this study was conducted to collect the biological information on insect pests that are essential for predicting potential damage caused by insect pests in future environment. In addition, we compared domestic and foreign research trends regarding climate change effect and suggested future research subjects. Domestic researchers were rather narrow in the subject, and were mostly conducted based on short-term monitoring data to determine relationship between insects and environmental variables. On the other hand, foreign researches studied on various subjects to analyze the effect of climate change, such as changes in distribution of insect using long-term monitoring data or their prediction using population parameters and models, and monitoring of the change of the insect community structure. To determine change of the phenology, distribution, overwintering characteristics, and genetic structures of insects under climate change through development of monitoring technique, in conclusion, further researches are needed. Also, development of population models for major or potential pests is important for prediction of climate change effects.

• Proceedings of the Korean Society of Plant Pathology Conference
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• 2003.10a
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• pp.108.2-109
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• 2003

The Gyeonggi-do Agricultural Research and Extension Services has developed a web-site (www.epilove.com) in collaboration with EPINET to provide information on agricultural weather and rice disease and insect pest management in Gyeonggi-do. Weather information includes near real-time weather data monitored by automated weather stations (AWS) installed at rice paddy fields of 11 Agricultural Technology Centers (ATC) in Gyeonggi-do, and weekly weather forecast by Korea Meteorological Administration (KMA). Map images of hourly air temperature and rainfall are also generated at 309m x 309m resolution using hourly data obtained from AWS installed at 191 locations by KMA. Based on near real-time weather data from 11 ATC, hourly infection risks of rice blast, sheath blight, and bacterial grain rot for individual districts are estimated by disease forecasting models, BLAST, SHBLIGHT, and GRAINROT. Users can diagnose various diseases and insects of rice and find their information in detail by browsing thumbnail images of them. A database on agrochemicals is linked to the system for disease and insect diagnosis to help users search for appropriate agrochemicals to control diseases and insect pests.

• Korean journal of applied entomology
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• v.54 no.2
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• pp.111-114
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• 2015

We report three lepidopteran insect pests of non-astringent persimmon leaf for the first time from Korea; Hypocala deflorata (Noctuidae), Teliphasa elegans (Pyralidae), and Cuphodes diospyrosella (Gracillariidae). Larvae of these species were collected from an organic farming or abandoned persimmon orchard in Changwon and Jinju cities, Gyeongnam province, and reared for the identification in the laboratory. Some information, such as collection records, hosts, simple morphological characteristics, and ecology were introduced for each species.

• Korean journal of applied entomology
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• v.22 no.2 s.55
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• pp.84-97
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• 1983

Under the limited arable land, the enhancement of agricultural productivity is indispensable to provide the food demand which is concomitant with the rapid increase in population. From this viewpoint, the upbringing and dissemination of high-yielding varieties has been promoted continuously and several modifications in cultural practices, including heavy fertilization, dense planting, and early transplanting, also have been gradually developed. However these changes in cultivation have led to the increased outbreak of insect pests and diseases. And this unexpected results have accelerated the number and complexity of pesticides employed as well as their consumption. Even though pesticides are essential materials contributing to the steady production of agricultural crops, large scale consumption of them has given rise to several adverse impacts, such as mammalian hazard and/or environmental contamination. In this respect, recent development of new pesticides has been concentrated on 'safe pesticide', as it were, that has the highly selective properties without unfavorable side influences on other ecosystem. According to literature cited up to now, feasibilities of safe pesticide development would be summarized as two categories. One of them is the development of chemical pesticides, which include the molecular structure modification of established pesticides for increased safety and synthesis of new safe chemicals which can attack the vulnerable point of physio-ecological characteristics in insect pests and diseases. The other is the biological pesticides which comprise natural enemies and microorganisms to act selectively on confined insect pests and diseases, In addition, improvement of physico-chemical properties of available pesticide formulations would be one of the desirable means for safe pesticide development in view of efficacy enhancement and minimization of hazardous properties or safe pesticide development, various approaches are feasible and needed to study, however, long period and much financial outlay are necessary to develop a new item. And under the present situation in Korea, there are many difficulties for performing research on all the possible routes. Therefore, combined pesticides by the reasonable combination of already registered resticides evaluated as the fairly safe pesticides and safe formulation based on their physico-chemical properties would be developed primarily. And many efforts would be given gradually for the development of new chemical and biological pesticides.