• 원예과학기술지
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• 제29권6호
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• pp.617-622
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• 2011

자연발생 배수체를 이용한 다양한 육종 소재를 개발함에 있어 그 효율성을 평가하고자 종자의배형성이 다른 일부 만다린 잡종에 대해 종자 형성 정도, 자연발생 배수체의 발생 빈도, 배수체의 생장 특성을 분석하였다. 다배성의 'Amakusa', 'Haruka', 'Hayaka' 및 'Seminole' 4품종과 단배성의 'Benibae'와 'Harehime' 2품종에 대해 방임수분된 과실로부터 종자를 채취하였다. 과실당 종자수는 10.0개이었고, 이 중 소형의 발육종자 형성 빈도는 25.1%이었다. 이들 소형 종자의 기내 발아 식물체에 대해 배수체 분석기 및 염색체 분석으로 배수성을 분석하고 배수체를 선발하였다. 'Harehime' 3배체 1개, 'Amakusa' 4배체 1개, 그리고 'Benibae' 4배체 1개가 각각 획득되었다. 이들 4배체와 3배체의 잎의 형태, 두께, 엽병 길이 및 절간장을 2배체와 비교하였는데, 큰 차이는 나타나지 않았다. 그러나 기공의 크기와 분포에 있어서는 분명한 차이를 나타내어 3 또는 4배수체 식물에서 기공의 크기가 커지고 분포 밀도는 감소하였다. 엽록소 함량을 나타내는 SPAD 값과 광합성 정도에 있어서는 큰 차이를 나타내지 않았다. 본 연구결과로 감귤 만다린 잡종에서 방임수분으로도 자연발생 배수체 생산이 가능하며, 다배성보다는 단배성에서 발생 빈도가 높은 것을 확인할 수 있었다.

• 문화경제연구
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• 제17권2호
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• pp.161-184
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• 2014

최근 역사적으로 의미를 가지는 근대건축문화유산을 활용한 체험전시관들이 부상하고 있는데, 본 연구에서는 문화자원을 활용한 지역 체험전시관이 경쟁력을 강화하기 위한 방안을 모색하고자 하였다. 이에 전북 완주군 삼례문화예술촌의 체험전시관을 대상으로 체험경제이론의 체험요소(4Es)에 따라 전시관의 성격을 분석하였다. 연구방법은 공무원, 체험관담당자, 전문가 세그룹을 대상으로 심층면접조사를 실시하였다. 분석결과 비쥬얼미디어아트미술관은 엔터테인먼트, 김상림목공소는 교육, 책박물관은 현실도피, 책공방북아트는 엔터테인먼트, 디자인뮤지엄은 심미, 문화카페오스는 심미로 나타났다. 또한 영리성을 추구하는 전시관은 김상림목공소, 책공방북아트센터, 책박물관, 문화카페오스이며 비영리성을 추구하는 전시관은 디자인뮤지엄, 비쥬얼미디어아트미술관으로 나타났다. 연구결과 지역체험전시관의 경쟁력 강화방안으로 첫째, 지역민의 문화향유를 위한 공공성 확보방안으로 작품 순환전시, 작품 보조설명 시설구축, 문화적 장소표현을 해야 한다. 둘째, 관광객확보를 통한 수익창출 방안으로 다양한 체험프로그램 제공 및 공간 확보, 기념품 샵 운영 등을 해야 한다. 셋째, 삼례체험전시관의 홍보마케팅 전략방안으로 모바일 앱 개발 및 SNS 등을 활용하여 다양한 이벤트를 실시해야 한다.

• Asian-Australasian Journal of Animal Sciences
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• 제27권11호
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• pp.1663-1670
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• 2014

The objective of this study was to determine the perceptions and knowledge of farmers of heat stress and tick resistance in cattle. A cross-sectional survey was conducted and 110 farmers in four villages in the sour and sweet velds of the Eastern Cape Province, South Africa were interviewed. The associations among area (municipality), gender, age, level of education, employment and religion were computed using Chi-square tests. The majority of the respondents had on average 4 bulls, 4 cows, 4 heifers, 4 calves, and 4 oxen. Milk was considered as the major (28.3%) reason for keeping cattle. Most farmers owned non-descript (72.6%), and Nguni (45.3%) cattle because of their heat tolerance (54.7%), tick resistance (54.7%), and milking ability (28.2%) traits. Excessive panting (56.6%) and disease transmission (76%) were regarded as the major effects of heat stress and tick infestation in cattle, respectively. About 50% of the respondents agreed that hair length influences tick resistance and 47.17% considered coat colour when acquiring cattle. In the sampled areas, ticks were prevalent in the summer season (93%), and 77.36% of the respondents use acaricides every fortnight. Gall sickness was reported to be a major problem in the cattle herds by 36.79% of the respondents. Our results showed that farmers in the two municipalities had knowledge of cattle adaptation to heat stress and tick resistance.

• 식물병연구
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• 제10권4호
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• pp.304-309
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• 2004

참다래 과수원에서 환상박피와 비가림시설은 꽃썩음병을 효과적으로 경감시켜주는 것으로 밝혀졌다. 참다래 개화기 약 45일 전인 4월 10일경에 참다래 주간부위에 20${\sim}$30 mm 폭으로 환상박피를 하면 꽃썩음병을 효과적으로 예방할 수 있었는데, 주간부위의 환상박피 높이는 방제효과에 영향을 미치지 않았다. 비닐을 이용한 비가림시설은 파풍망시설보다 비가림방식에 관계없이 꽃썩음병 예방에 효과적이었다. 참다래 개화기 약 75일 전인 3월 10일경에 부분비닐피복식 비가림시설을 하면 참다래 꽃썩음병의 발생을 거의 완벽하게 방제할 수 있었다. 환상박피와 부분비닐피복식 비가림시설은 참다래 과수원에서 환경친화적인 꽃썩음병 방제방법으로 실용화할 수 있을 것으로 기대된다.

• 식물병연구
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• 제10권4호
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• pp.297-303
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• 2004

참다래 꽃썩음병은 주로 항생제 살포에 의해 방제되고있다. 스트랩토마이신황산염 옥시테트라사이클린 수화제, 농용신 쿠퍼 수화제와 옥쏘리닉에시드 수화제가 15개의 후보약제들 대상으로 한 실내시험 및 포장시험을 통하여 참다래 꽃썩음병 예방약제로 선발되었다. 참다래 꽃썩음병 방제를 위하여 스트랩토마이신황산염 옥시테트라사이클린 수화제와 농용신 쿠퍼 수화제의 살포적기는 개화기이고, 여러 가지 살포시기별 다양한 약제살포 조합 중에서 참다래 꽃썩음병 방제를 위한 스트랩토마이신황산염 옥시테트라사이클린 수화제와 농용신 쿠퍼 수화제의 최적 살포 회수는 참다래 개화기인 5월 초부터 10일 간격으로 3회로 판명되었다.

• The Plant Pathology Journal
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• 제24권2호
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• pp.125-130
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• 2008

Various kinds of leaf spots and blights were found in kiwifruit (Actinidia deliciosa) orchards on 2006 in Korea. Disease incidences were quite variable between open-field and rain-proof shelter. Rate of diseased leaves was recorded as about 70% at open-field orchards in late season but use of rain-proof vinyl shelters alleviated the disease incidences by 20%. Angular leaf spots appeared at early infection stage on June and several other symptoms were also recognized as the disease developed afterward. On September, brown leaf blights were the most frequent, followed by grayish brown ring spots, silvering gray leaf blights, zonate leaf blights, dark brown ring spots and angular leaf spots at open-field orchards. Four fungal species were frequently isolated from the disease symptoms. Phomopsis sp. was the most predominant fungus associated with the leaf spot and blight symptoms on kiwifruit, followed by Glomerella cingulata, Alternaria alternata and Pestalo-tiopsis sp. Phomopsis sp. was commonly isolated from angular leaf spots, silvering gray leaf blights, and zonate brown leaf blights. G. cingulata, A. alternata and Pestalotiopsis sp. were isolated from grayish brown ring spots (anthracnose), brown ring spots and zonate dark brown leaf blights. Typical symptoms appeared on the wounded and unwounded leaves, which were inoculated by each of Phomopsis sp., G. cingulata, and Pestalotiopsis sp., but A. alternata caused symptoms only on the wounded leaves.

• Journal of Ginseng Research
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• 제38권1호
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• pp.52-58
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• 2014

To determine whether Fourier transform (FT)-IR spectral analysis combined with multivariate analysis of whole-cell extracts from ginseng leaves can be applied as a high-throughput discrimination system of cultivation ages and cultivars, a total of total 480 leaf samples belonging to 12 categories corresponding to four different cultivars (Yunpung, Kumpung, Chunpung, and an open-pollinated variety) and three different cultivation ages (1 yr, 2 yr, and 3 yr) were subjected to FT-IR. The spectral data were analyzed by principal component analysis and partial least squares-discriminant analysis. A dendrogram based on hierarchical clustering analysis of the FT-IR spectral data on ginseng leaves showed that leaf samples were initially segregated into three groups in a cultivation age-dependent manner. Then, within the same cultivation age group, leaf samples were clustered into four subgroups in a cultivar-dependent manner. The overall prediction accuracy for discrimination of cultivars and cultivation ages was 94.8% in a cross-validation test. These results clearly show that the FT-IR spectra combined with multivariate analysis from ginseng leaves can be applied as an alternative tool for discriminating of ginseng cultivars and cultivation ages. Therefore, we suggest that this result could be used as a rapid and reliable F1 hybrid seed-screening tool for accelerating the conventional breeding of ginseng.

• 대한약침학회지
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• 제22권3호
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• pp.140-146
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• 2019

Objectives: Venomous fishes have different pharmacological effects and are useful. Among the venomous fish, stonefishes; especially Pseudosynanceia melanostigma has various pharmacological effects on the nervous, muscular and cardiovascular system of humans. In this study, toxicological characteristics, some blood effects, pharmacological and enzymatic properties of Pseudosynanceia melanostigma venom was investigated. Methods: Crude venom purified by using gel filtration chromatography and the molecular weights of the venom and its fractions were estimated. The approximate LD values of this venom were determinedand the effects of LD50 dose on the blood of rabbits were studied. Hemolytic and Hemorrhagic activity of the venom sample was determined. In this case coagulation tests were performed. Results: The LD50 of the Pseudosynanceia melanostigma crude venom was also determined to be $194.54{\mu}g/mouse$. The effect of two doses of LD50 showed a non-significant differences decrease in RBCs and MCV. In other cases, the results showed significant differences in WBC, Plt, Hb, MCH, MCHC and HCT; also it's showed a significant decrease. WBC count showed a significant increase with two doses of LD50 groups. The prothrombin time and partial prothrombin time were increased after venom treatment. As well as bleeding and clotting time were increased. According to the results, a minimum dose for Haemorrhagic effect $40{\mu}g$ was obtained. Conclusion: Venom of Pseudosynanceia melanostigma has inhibitory effect on platelet aggregation that can be used to design and develop of anticoagulant drugs.

• 식물분류학회지
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• 제52권4호
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• pp.226-234
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• 2022

The study aims are to examine the characteristics of artificial and natural hybrids between Viola albida var. albida (= albida, from below) and V. albida var. chaerophylloides (= chaerophylloides, from below), and to confirm if hybrids could be fertile and make populations in their native habitats. The 1st filial (= F₁, from below) leaf shape produced by artificial crossing between albida and chaerophylloides was the same as that of V. albida var. takahashii (= takahashii, from below), and F₁ bore also both chasmogamous and cleistogamous flowers. F₁ seed number was 9.6 per cleistogamous pods, which was remarkably less than the average of 38.2 for albida and chaerophylloides, but the germination rate was all similar. The leaf type of self-crossed 2nd offsprings (= F₂, from below) showed all leaf types found in the Viola albida complex, but the ratio of chaerophylloides leaf type was relatively low. Individuals whose F₂ leaf type was restored to albida produced an average of 31.4 seeds per capsule, meaning that fertility was restored. On the other hand, individuals of F₂ takahashii leaf type come to fruition a low average of 10.4 seeds per capsule, which is similar to that of takahashii. The results of crossbreeding experiment, where is their native habitats, were similar to that of laboratory. Both albida and chaerophylloids in Mt. Bulmyeong distribute extensively, but takahashii make a small population only in places where albida and chaerophylloides grow together. Summarizing the above results is suggesting that the speciation of takahashii was done by hybrid between albida and chaerophylloides, and these have been maintained with relatively small population by cleistogamous capsules.

• Journal of Plant Biotechnology
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• 제44권1호
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• pp.12-18
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• 2017

당귀는 우리나라를 포함하여 중국과 일본 등 아시아국가에서 유용하게 이용되는 한약재이다. 그러나 국가마다 그 기원을 달리하기 때문에 혼 오용이 심하고 국제시장에서 혼란을 불러일으킬 소지가 다분하다. 따라서 오래전부터 형태학적, 세포유전학적 분석과 지표성분을 이용한 화학적 판별 마커의 개발에 관한 연구가 많이 진행되어 왔다. 또한 최근에는 다양한 재배환경과 수확 후 가공 및 처리방법에도 비교적 안전한 유전자 단편의 염기서열 비교분석을 통한 분자생물학적 기술을 적용한 판별기술의 개발에 관한 연구결과 들이 발표되고 있다. 그러나 아직까지 이들 기술의 실용화를 통한 현장 적용에는 한계가 있으며 보다 많은 후속연구가 수행되어야 한다. 이에 본 논문에서는 현재까지의 연구결과를 바탕으로 얻어진 문제점을 논하고 향후 필요한 추가 연구 과제들에 관하여 기술하였다.