• Asian-Australasian Journal of Animal Sciences
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• 제20권8호
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• pp.1169-1173
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• 2007

The protein encoded by SLC27A2 gene is an isozyme of long-chain fatty-acid-coenzyme A ligase family, and it converts free long-chain fatty acids into fatty acyl-CoA esters, and thereby plays a key role in lipid biosynthesis and fatty acid degradation. In the present study, SLC27A2 located on human chromosome 15 was selected as candidate gene and we isolated and cloned partial fragments of mRNA sequence and genomic fragments of porcine SLC27A2 gene. The coding region of the gene as determined by alignments shared 90% and 82% identity with human and mouse cDNAs, respectively. Detection in LargeWhite and Meishan breeds showed that a single nucleotide polymorphism (SNP) ($A{\rightarrow}G$) existed in exon 7, which caused corresponding amino acid changed for encoding. In LargeWhite pigs it encoded for Val while in Meishan pigs it encoded for Ile, so we developed the PCR-RFLP genotype method for detection of this polymorphism. Association study in 135 $F_2$ reference family indicated that significant correlation existed between the polymorphism and growth and carcass traits.

• Asian-Australasian Journal of Animal Sciences
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• 제21권7호
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• pp.954-960
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• 2008

MiRNAs (microRNAs) are a class of small non-coding RNA molecules of ~21 nucleotides that down- regulate the expression of target genes at post-transcriptional level. In this study, we first accomplished a preliminary scan of miRNA expression using 65 and 90 day fetal pig skeletal muscle samples by microarray hybridization, and 34 miRNAs showed strong positive signals. Five of these miRNAs were selected for further investigation by real-time RT-PCR. The statistical analyses indicated that three miRNAs exhibited significant differential expression (p<0.05) during porcine muscle development from 65 to 90 days of gestation, e.g., miR-24 and miR-424 were down-regulated while miR-133a was up-regulated. Multi-tissue RT-PCR was performed to detect the expression patterns of the five miRNA precursors. The results showed that most of these precursor miRNAs were ubiquitously expressed in different porcine tissues.

• 농촌계획
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• 제28권1호
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• pp.71-79
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• 2022

Recently, alarming rates of climate change have amplified the importance of water conservation in the agricultural sector; emphasizing the implementation of an integrated approach as the principal water management strategy. As part of comprehensive measures to cope with drought in the agricultural and rural sectors, water-saving education models and pilot projects for farmers' education have been implemented since 2016, but these activities were launched as a one-time project in limited areas. Therefore, systematic and extensive efforts are required to highlight the site-specific and tangible benefits of water-saving education and public relations projects. As part of this study, educational sessions and surveys were conducted in the Yeoju-Icheon, Seosan-Taean, and Gyeongju areas to reflect the on-site applicability of the water-saving education model. Based on the survey of previous studies, a total of 16 water-saving effectiveness factors were developed and the effectiveness was derived for each factor. As a result of farmer education, the overall effectiveness evaluation score of 1.13 increased to 3.34, and the effectiveness of each factor was also improved. These water-saving education effectiveness factors can be used as basic data for determining and launching future policies or systems to improve the water-saving education of farmers. In addition, this study raises the need to prepare feasible related systems that can lead farmers to actively participate in water management and conservation and to promote systematic and continuous water conservation education and promotion policies.

• 농촌지도와개발
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• 제19권2호
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• pp.273-299
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• 2012

This study examined importance and performance of urban agriculture education program of the trainees who attended urban agriculture education, and suggested reformation of urban agriculture instructor course of Gyeonggi Provincial Agricultural Research & Extension Services by IPA models. The investigation was done on October 7, 2011. The subject was 40 trainees of urban agriculture instructor course of Gyeonggi Provincial Agricultural Research & Extension Services. The study suggested reformation of urban agriculture education program based on the findings as follow: Firstly, the instructors should teach trainees not theoretically but practically to let the trainees make use of learning at urban agriculture. Secondly, teaching material that is not theoretically but practical enough to apply it to actual urban agriculture should be supplied. Thirdly, urban agriculture education should be done not theoretically but practically considering its characteristics. Theoretical contents have been already included in teaching material, and practice oriented education should be done to let trainees put theory into practice on-the-spot. Practice oriented education should be supported in the planning future education. In addition future urban agriculture education program should consider importance as well as practice of educational contents, lecturers, operations and facilities.

• 농촌지도와개발
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• 제25권2호
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• pp.85-97
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• 2018

This study aims to investigate the farmer's educational performances Influencing educational satisfaction. In general, the goal of agricultural education is to increase farm performance such as income, sales and perceptions. A survey was used to collect data from 588 farmers to the country extension office. The results of the analysis are as follows. The variables that have a significant effect on satisfaction in this study are as follows. interaction between instructors and farmers, passion of instructors, effectiveness of lectures, educational support, and educational facilities. However, the adequacy of the lecture and the diversity of the contents of education did not explain the satisfaction variable. Discussions on the improvement of farmers' education were made by considering the rejected variables. In conclusion, farmers' education in the field should secure educational diversity. And farmer's education in RDA should develop a new curriculum. Farmer's education in RDA should further strengthen the informal learning.

• 농촌지도와개발
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• 제6권2호
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• pp.85-103
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• 1999

The objectives of the study were to review the agricultural environment education program for nasal change agents, to find out their recognition on agricultural environment and to get sorn implications for better educational program of rural environment. The data were gathered from 177 rural change agents in 11 agricultural technology centers in Kyongi-Do province by mailing survey with questionnaire. The major findings of the study were ; (1) Agricultural environment education for rural extention workers initiated in 1996. From 1999 diversified programs were served for them. (2) Rural change agents perceived that water contamination and degradation of life environment were the most serious problem. (3) Educational program on organic farming should be prepared for farmers and rural changes agents. (4) Concrete educational programs for adequate use of agricultural chemicals should be prepared for farmers. (5) Educational program on agricultural environment policies should be reorganized in terms of the level of target group. (6) RDA should prepare synthetical education program for the conservation of rural eco-system.

• 농업과학연구
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• 제49권2호
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• pp.259-267
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• 2022

Although pesticides are recognized as necessary substances to improve agricultural production, exposure to pesticides is known to have a direct or indirect adverse effect on the reproductive function of mammals. The present study examines the effects of mancozeb, a well-known fungicide, on the fertility capacity of spermatozoa. Boar spermatozoa exposed to varying concentrations of mancozeb (0.01 - 0.5 µM) were evaluated for motility, motion kinetic parameters, viability, acrosome integrity and the generation of intracellular reactive oxygen species (ROS) after 30 min or 2 hrs of incubation. A significant reduction in the motility of spermatozoa was observed upon exposure to mancozeb. Similarly, there was a significant reduction of the motion kinematics of sperm treated with mancozeb as compared to untreated controls (p < 0.05). The sperm viability percentage and acrosome integrity also showed dose-dependent decreases upon exposure to mancozeb. High concentrations of mancozeb (0.2 - 0.5 µM) induced higher levels of intracellular ROS production, which resulted in the loss of the sperm membrane and decreased sperm motility due to oxidative stress. Taken together, the results here indicate that direct exposure to mancozeb affects the sperm fertility capacity. Hence, careful research that examines the interaction between reproduction and environmental toxins is crucial to prevent fertility disorders in animals.

• 한국유기농업학회지
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• 제26권3호
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• pp.327-349
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• 2018

The purpose of the study focuses on the agriculture education services in the changing rural areas conditions such as population decline, aging society, and returning farmers. The study reviews the effects of agricultural education services on returning farmers and local residents for satisfaction, intention for recommendation, and intention to continue participation. Further, the study aims to investigate any difference in the level of satisfaction for two groups. The results suggested that there is a meaningful difference between return-farmers and local residents. Among the demographic variables, age and income showed a notable difference. However, sex, level of education and type of household did not suggest noticeable differences. In addition, the study accessed agricultural education from a service perspective and analyzed its service quality and customer satisfaction, loyalty and relationship using a service profit chain model. Like the result of most other studies, the analysis showed that these had positive relationships. While the study focused on the efficiency of agriculture education training program in agriculture technology centers, the study carries a meaningful value in that it discovered a meaningful difference in the satisfaction level between returning farmers and locals despite the fact that agriculture education was applied as a part of service. In practical terms, the study pointed out the need for consumer-centered education that reflects the characteristics of the groups rather than standardized education.

• 농촌지도와개발
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• 제22권1호
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• pp.79-92
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• 2015

Agricultural Technology Center is an education center to provide agriculturalists with information and technology related with agriculture. As domestic and international environment of agriculture and rural area is changing, functions and roles of Agricultural Technology Center are evolving according to stream of times. In 1995, a new urban and rural complex city was created for symbiotic relationship between city and rural area, As a result, user group of Agricultural Technology Center expanded to nonagriculturalists. Though the change of agricultural extension service is necessary to perform extended functions of rural area and to satisfy needs of new group of users, it is difficult to find enough studies on new functions and roles of Agricultural Technology Center to manage the service change. Therefore, this study is aimed to suggest new functions and roles of Agricultural Technology Center according to stream of times. This study were surveyed through questionnaire targeting users of Namyangju Agricultural Technology Center to compare user group awareness of Agricultural Technology Center in urban and rural complex city. According to this study result, while main purpose of agriculturalists' visiting at Agricultural Technology Center was participating in education programs related with agriculture. In contrast, nonagriculturalists usually visited the center for agricultural understanding, leisure activities, and children education, participating in field work programs. From the survey result of required functions of the center, it was revealed that nonagriculturalists expected urban agriculture, research for living improvement in rural area, adjustment education for returning farmer, and function of farming promotion, comparing agriculturalists. It is verified that this difference of user group awareness reflects the necessity that Agricultural Technology Center should change services and accept increasing use of nonagriculturalists with new functions and roles.

• 농업과학연구
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• 제49권1호
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• pp.103-112
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• 2022

Fipronil is a popular insecticide used in both agricultural and domestic fields. Factors that affect sperm and eggs have a direct influence on reproductive outcomes. This study was undertaken to assess the effect of varying concentrations (10 - 200 μM) of fipronil and incubation times (30 min and 2 hrs) on boar spermatozoa. Spermatozoa were evaluated for motility, motion kinematics, viability, chromatin stability, and for the generation of intracellular reactive oxygen species (ROS) and the results were compared to those from corresponding controls. The findings revealed a significant, dose-dependent reduction in sperm motility in all fipronil treatment groups at 30 min of incubation (p < 0.05). A similar dose-dependent reduction in sperm motility was observed subsequent to fipronil exposure for 2 hrs of incubation (p < 0.05). Groups treated with fipronil showed a gradual reduction in motion kinematics (p < 0.05). Moreover, a significantly higher percentage of dead sperm was observed at 200 μM fipronil, as compared to the highest live percentage obtained in controls (p < 0.05). Evaluating the sperm chromatin integrity revealed a significantly higher percentage of damaged chromatin in spermatozoa incubated with 200 μM of fipronil. Moreover, ROS production was significantly higher in fipronil-exposed sperm (p < 0.05). In conclusion, boar spermatozoa incubated with fipronil showed decreased levels of sperm motility and viability, weaker chromatin integrity, and increased levels of intracellular ROS generation, all of which indicate that exposure to fipronil potentially impairs the fertilization competence of boar spermatozoa.