• 제목/요약/키워드: agricultural crop

검색결과 6,169건 처리시간 0.04초

Stem Rot on Adzuki Bean (Vigna angularis) Caused by Rhizoctonia solani AG 4 HGI in China

  • Sun, Suli;Xia, Changjian;Zhang, Jiqing;Duan, Canxing;Wang, Xiaoming;Wu, Xiaofei;Lee, Suk-Ha;Zhu, Zhendong
    • The Plant Pathology Journal
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    • 제31권1호
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    • pp.67-71
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    • 2015
  • During late August and early September 2011, stem rot symptoms were observed on adzuki bean plants (Vigna angularis) growing in fields located in Beijing and Hebei Province, China, respectively. In this study, four isolates were obtained from infected stems of adzuki bean plants. Based on their morphology, and sequence and polymerase chain reaction-restriction fragment length polymorphism (PCR-RFLP) analyses of the ribosomal DNA internal transcribed spacers (rDNA-ITS) region, the four isolates were identified as Rhizoctonia solani in anastomosis group (AG) 4 HGI. Pathogenicity tests showed that all isolates were strongly pathogenic to adzuki bean and resulted in serious wilt symptoms which was similar to observations in the fields. Additionally, the isolates infected several other crops and induced related rot on the roots and basal stems. To our knowledge, this is the first report of Rhizoctonia solani AG 4 HGI causing stem rot on adzuki bean.

Discrimination and Detection of Erwinia amylovora and Erwinia pyrifoliae with a Single Primer Set

  • Ham, Hyeonheui;Kim, Kyongnim;Yang, Suin;Kong, Hyun Gi;Lee, Mi-Hyun;Jin, Yong Ju;Park, Dong Suk
    • The Plant Pathology Journal
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    • 제38권3호
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    • pp.194-202
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    • 2022
  • Erwinia amylovora and Erwinia pyrifoliae cause fire blight and black-shoot blight, respectively, in apples and pears. E. pyrifoliae is less pathogenic and has a narrower host range than that of E. amylovora. Fire blight and black-shoot blight exhibit similar symptoms, making it difficult to distinguish one bacterial disease from the other. Molecular tools that differentiate fire blight from black-shoot blight could guide in the implementation of appropriate management strategies to control both diseases. In this study, a primer set was developed to detect and distinguish E. amylovora from E. pyrifoliae by conventional polymerase chain reaction (PCR). The primers produced amplicons of different sizes that were specific to each bacterial species. PCR products from E. amylovora and E. pyrifoliae cells at concentrations of 104 cfu/ml and 107 cfu/ml, respectively, were amplified, which demonstrated sufficient primer detection sensitivity. This primer set provides a simple molecular tool to distinguish between two types of bacterial diseases with similar symptoms.

유기농업의 소득 극대화를 위한 작부체계 수립 전략 (Establishing a Crop System of Organic Farming for Maximizing Agricultural Income)

  • 김호;김성태
    • 한국유기농업학회지
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    • 제20권2호
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    • pp.143-159
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    • 2012
  • Agricultural income is calculated with producer price, output and management cost. This study compared organic farming with conventional one for agricultural income, producer price and output by items. And then it proposed the method of item selection and crop system from a diversification point of view. The coefficient of variation to producer prices in organic farming was 4.7%, and conventional one was 30.3% because organic products have been produced in a system of contract farming with consumers' cooperative. This result means the price of organic products is stabler than that of conventional price. And agricultural income of organic farming has been generally known more than that of conventional one. However, agricultural gross income of conventional farming was more than that of organic one by 20.3% in 2010. It was caused by output reduction of a few items(fer example; onion, large green onion, potato and young pumpkin) due to freak weather conditions and constant producer price for several years in organic farming. In order to increase agricultural income, appropriate crop selection and system should be introduced to organic farming. A principal crop is the rice plant and 2 subordinate crops are dry crops at bare field and greenhouse respectively. Thus 5 crop systems that agricultural gross income are relatively increased larger among 15 crop systems estimated are rice+ginger+cucumber, rice+ginger+tomato, rice+large green onion+cucumber, rice+sweet potato+cucumber and rice+onion+ cucumber.

Antioxidant Activities and Determination of Phenolic Acids from Leaves of Perilla frutescens

  • Lee, Jin-Hwan;Kang, Nam-Suk;Ha, Tae-Joung;Ko, Jong-Min;Han, Won-Young;Suh, Duck-Yong;Park, Ki-Hun;Baek, In-Youl
    • Journal of Applied Biological Chemistry
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    • 제49권1호
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    • pp.11-15
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    • 2006
  • Two catecholic phenolic acids (1 and 2) obtained from 80% methanolic extract of Perilla frutescens var. frutescens leaves through chromatography showed strong DPPH (1: $IC_{50}$ = 15.5 ${\mu}M$, 2: $IC_{50}$ = 11.7 ${\mu}M$) and ABTS (1: $IC_{50}$ = 5.5 ${\mu}M$, 2: $IC_{50}$ = 4.6 ${\mu}M$) radicals scavenging abilities. Antioxidant compounds contents of 1 and 2 as determined by $C_{18}$ reversed phase HPLC coupled with diode-array detector were 2.98 and 2.26 mg/g, respectively.

Isolation and Characterization of Rice OsHRL Gene Related to Bacterial Blight Resistance

  • Park, Sang-Ryeol;Moon, Seok-Jun;Shin, Dong-Jin;Kim, Min-Gab;Hwang, Duk-Ju;Bae, Shin-Chul;Kim, Jeong-Gu;Yi, Bu-Young;Byun, Myung-Ok
    • The Plant Pathology Journal
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    • 제26권4호
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    • pp.417-420
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    • 2010
  • The expression of HR-like lesion inducing gene of Oryza sativa (OsHRL) was slightly increased by Xanthomonas oryzae pv. oryzae (Xoo) infection. Transgenic rice plants over-expressing OsHRL gene were challenged with Xoo and the development of disease symptoms were examined to investigate the effect of OsHRL gene expression on plant defense responses. The over-expression of OsHRL increased disease resistance against Xoo compared with wild type plants.

Isolation and Characterization of Phytochemical Constituents from Soybean (Glycine max L. Merr.)

  • Lee, Jin-Hwan;Baek, In-Youl;Kang, Nam-Suk;Ko, Jong-Min;Han, Won-Young;Kim, Hyun-Tae;Oh, Ki-Won;Suh, Duck-Yong;Ha, Tae-Joung;Park, Ki-Hun
    • Food Science and Biotechnology
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    • 제15권3호
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    • pp.392-398
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    • 2006
  • Four flavonoids 1-4 and one phytosterol 5 were isolated from methanol extract of Taekwangkong, one of the soybean cultivars, and the structures of these compounds were fully characterized by physical and spectral analysis. The content of compounds 1-4 as determined by $C_{18}$ reversed phase HPLC (high-performance liquid chromatography) coupled with diode-array detector were 12.1, 624.6, 18.0, and $219.6\;{\mu}g/g$, respectively, and the total phenolic content of this cultivar was measured as 3.7 mg gallic acid equivalent per g dry material (GAB/g). Also, compound 1 showed strong radical scavenging activity in the 2,2'-azino-bis-(3-ethylbenzthiazoline-6-sulfonic acid) (ABTS) assay ($IC_{50}\;=\;47.6\;{\mu}M$), five-fold higher than seen in the 1,1-diphenyl-2-picrylliydrazyl (DPPH) assay. These results lead to the conclusion that soybean not only has many phytoestrogens but also has potent antioxidant activity.