The Plant Pathology Journal

The Korean Society of Plant Pathology (한국식물병리학회)
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Discrimination and Detection of Erwinia amylovora and Erwinia pyrifoliae with a Single Primer Set

  • Ham, Hyeonheui (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kim, Kyongnim (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Yang, Suin (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Kong, Hyun Gi (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Lee, Mi-Hyun (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Jin, Yong Ju (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration) ;
  • Park, Dong Suk (Crop Protection Division, Department of Agro-food Safety and Crop Protection, National Institute of Agricultural Sciences, Rural Development Administration)
  • Received : 2022.03.03
  • Accepted : 2022.04.13
  • Published : 2022.06.01
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Abstract

Erwinia amylovora and Erwinia pyrifoliae cause fire blight and black-shoot blight, respectively, in apples and pears. E. pyrifoliae is less pathogenic and has a narrower host range than that of E. amylovora. Fire blight and black-shoot blight exhibit similar symptoms, making it difficult to distinguish one bacterial disease from the other. Molecular tools that differentiate fire blight from black-shoot blight could guide in the implementation of appropriate management strategies to control both diseases. In this study, a primer set was developed to detect and distinguish E. amylovora from E. pyrifoliae by conventional polymerase chain reaction (PCR). The primers produced amplicons of different sizes that were specific to each bacterial species. PCR products from E. amylovora and E. pyrifoliae cells at concentrations of 104 cfu/ml and 107 cfu/ml, respectively, were amplified, which demonstrated sufficient primer detection sensitivity. This primer set provides a simple molecular tool to distinguish between two types of bacterial diseases with similar symptoms.

Keywords

Acknowledgement

This work was supported by the "Cooperative Research Program for Agriculture Science and Technology Development" (Project No. PJ01421904), Rural Development Administration, Republic of Korea.

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