• Fisheries and Aquatic Sciences
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• 제11권4호
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• pp.224-228
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• 2008

With the aim of increasing the $CoQ_{10}$ production in mass culture, the effect of aeration-agitation on the $CoQ_{10}$ production using Rhodobactor sphaeroides was investigated in a l-L bioreactor. The maximum $CoQ_{10}$ production was 1.69 mg/g of dry cell weight under conditions of 50 Lux, $30^{\circ}C$, 300 rpm, and 5-vvm aeration. The $CoQ_{10}$ production was improved to produce 2.91 mg/g of dry cell weight under reduced conditions of agitation speed (200 rpm) and aeration rate (0.2 vvm). When R. sphaeroides was cultivated under more reduced DO levels during the exponential phase of the cell, the $CoQ_{10}$ production yield of 3.88-mg/g dry cell weight was the maximum obtained. Therefore, poorer conditions of aeration-agitation resulted in higher production of $CoQ_{10}$, and thus DO content was a crucial factor in the production of $CoQ_{10}$. Accordingly, it was necessary to control the DO concentration in order to enhance the $CoQ_{10}$ biosynthesis within a large-scale production.

• Journal of Electrical Engineering and Technology
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• 제3권2호
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• pp.271-275
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• 2008

The MAM(Megasonic Agitated Module) has been fabricated for improving the characteristics of wet etching. The characteristics of the MAM are investigated during the wet etching with and without megasonic agitation in this paper. The adoption of the MAM has improved the characteristics of wet etching, such as the etch rate, etch uniformity, and surface roughness. Especially, the etching uniformity on the entire wafer was less than ${\pm}1%$ in both cases of Si and glass. Generally, the initial root-mean-square roughness($R_{rms}$) of the single crystal silicon was 0.23nm. Roughnesses of 566nm and 66nm have been achieved with magnetic stirring and ultrasonic agitation, respectively, by some researchers. In this paper, the roughness of the etched Si surface is less than 60 nm. Wet etching of silicon with megasonic agitation can maintain nearly the original surface roughness during etching. The results verified that megasonic agitation is an effective way to improve etching characteristics of the etch rate, etch uniformity, and surface roughness and that the developed micromachining system is suitable for the fabrication of devices with complex structures.

• KSBB Journal
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• 제15권3호
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• pp.280-284
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• 2000

The simple imcroprocessor device was provided for the control of dissolved oxygen(DO) level. The DO analog signal output from fementer was used as a measurement variable and the agitation speed of fermenter was used as a manipulatio variable. Agitation speed was manipulated to maintain DO level above 10% of saturation condition during cell growth period with microprocessor. Since the experimental resultsshowed that the DO level was maintained above 10% and agitation speed was inverse proportional to DO level ithis simple control device can be used for the DO control of fermenter.

• KSBB Journal
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• 제16권3호
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• pp.307-313
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• 2001

The effects of agitaion and aeration on mycelial growth, exo-polysaccharide (EPS) production, and substrate consumption upon the submerged cultivation of G. lucidum were investigated, and the batch kinetics of the EPS fermentation of G. lucidum were interpreted as function of agitation speed and aeration rate. In a 2.6 L jar fermenter system, the optimum agitation speed and aeration rate for EPS production were determined to be 400 rpm and 1.0 vvm, respectively. The maximum production of EPS obtained was 15 g/L. The logistic model for mycelial growth fitted the experimental data better than that determined by the Monod and the two-thirds power models. The Luedeking-Piret equation adequately modelled the kinetic data obtained for product and substrate.

• 한국표면공학회지
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• 제34권6호
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• pp.545-552
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• 2001

Effect of ultrasonic agitation on Pd catalyst treatment was studied in metallization of ceramic boards by Cu electroless plating method.96% $Al_{2}$$O_{3}$ ceramic boards were used as substrate. In this study, the ultrasonic frequency of 28kHz was applied. In Pd catalyst, high density Pd nuclei of small size were formed during ultrasonic agitation. Density of Pd was more improved when using of ultrasonic then no stirring. In electroless plating, plating rate was in the range of 0.6~1.8$\mu\textrm{m}$/hr, which value increased with Rochelle Salts addition. Adhesion strength between ceramic boards and Cu layer was improved of 20% when using ultrasonic agitation at $30^{\circ}C$ ,5min.

• 한국생물공학회:학술대회논문집
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• 한국생물공학회 2000년도 추계학술발표대회 및 bio-venture fair
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• pp.334-337
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• 2000

Extracellular polysaccharide was produced by Pseudomonas elodea NK-2000 under nitrogen limitation and aerobic condition, The effect of agiatation speed on cell growth and production of exopolymer was investigated. The agitation speed of 7.5 L fermentor ranged from 200 to 500 rpm. Production of exopolymer increased with higher agitation speed. Maximal cell growth and production of exopolymer from 2% glucose were 3.35 g/l and 3.80 g/l, respectively when agitation speed was 400 rpm.

• Biotechnology and Bioprocess Engineering:BBE
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• 제10권6호
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• pp.571-575
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• 2005

A simple, but effective on-line method for estimating the mycelial cell mass concentration from agitation speed data, a most readily-available process variable, has been developed for DO-stat cultures of Agaricus blazei. The dynamic change of dissolved oxygen concentration (DOC) in the initial transient period and the change in yield were considered in the development of the estimation algorithm or estimator. Parameters in the estimation algorithm were calculated from the agitation speed data at 20% of DOC. The proposed estimator could accurately predict the cell mass concentration regardless of DOC levels in the tested range of $10{\sim}40%$, showing a good extrapolation capability.

• 대한화학회지
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• 제17권3호
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• pp.213-223
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• 1973

透析液 및 溶媒液의 攪拌이 透膜面에 미치는 流體力學的인 壓力分布狀態를 究明하였고, 攪拌으로 招來되는 透膜面兩面의 壓力不均衡을 除去할 目的으로 攪拌裝置의 開發을 試圖하였다. 다음 攪拌速度가 透析係數에 미치는 影響을 究明하여 가장 正確한 透析係數를 求할 수 있는 攪拌速度를 確定시켰다.

• 한국결정성장학회지
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• 제9권2호
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• pp.191-196
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• 1999

수열합성법을 이용한 $\alpha$-$SiO_2$ 단결정 분말 제조시 교반의 추가와 승온속도의 변화가 입자의 결정성, 입도분포 및 평균입경에 미치는 영향을 조사하였다. KOH를 광화제로 사용하였으며, $350^{\circ}C$에서 3시간 동안 반응을 시켜 평균입자 크기가 1~3.2$\mu\textrm{m}$인 결정 분말을 제조하였다. 실험결과 반응초기부터 교반을 한 경우에는 교반속도가 증가함에 따라 평균입경이 감소했으나, 결정성은 나빠지다가 150r/min 이상에서는 다시 좋아졌다. 또한 반응 도중에 교반을 추가하면 반응초기부터 교반을 한 경우보다 입자의 크기가 커졌다. 그러나 교반속도를 일정하게 한 후 승온속도를 느리게 하면 입자의 크기가 작아졌다.

• 한국환경과학회지
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• 제10권1호
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• pp.65-71
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• 2001

HRP(high rate pond) which had kept the manufactured clay of 3cm-thickness as benthic clay in reactor and the 6 flat-blade turbine as impeller for agitation was named HRASP(high rate algae stabilization pond). And the experiment for treatment of artificial synthesis wastewater containing COD :300mg/$\ell$, NH$_3$-N : 300mg/$\ell$, T-P : 9mg/$\ell$ as nutrients was been performed successfully. This reactor was been operated under conditions : 24hrs.-irradiation and water temperature, $25^{\circ}C$ and pH 7 and agitation velocity, 15, 30, 45rpm and the effect of agitation velocity on algal bioaccumulation of nutrients was been studied with view point of fluid dynamics. The next followings could be obtained as results. 1. The agitation with a turbine impeller blade in HRASP makes clay particle indicate superior suspension effect by means of forming of excellent curl/shear flow in reactor. 2. The excessive suspension of clay particle which is created at 45rpm as rotation velocity of impeller blade of turbine disturbs the light penetration and algal photosynthesis reaction. 3. Efficiencies for removal of nutrients come out as COD : 93.9%~94.3%, ($NH_3-N + NO_3-N$) : 81.9%~99.0%, T-P : 46.8%~53.6%. 4. Kuo values of $K_1$for algal growth come out seperately as 15rpm : $1.876{\times}10^{-2}, 30rpm : 4.618{\times}10^{-3}$. 5. Kuo values of $K_2$for removal of N, P come out seperately as 15rpm : $8.403{\times}10^{-1}$ and $1.397{\times}10^{-1}$, 30rpm : $4.823{\times}10^{-1} and 2.052{\times}10^{-1}$. 6. It can be guessed easily that the excessive agitation can inhibit the algal and bacterial symbiotic reaction if it is considered that micro organism\` sense to preservation of life is relied on natural function of metabolism. Therefore the studies for this matter should be followed continuously.