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On-Line Estimation of Cell Growth from Agitation Speed in DO-Stat Culture of a Filamentous Microorganism, Agaricus blazei  

Na, Jeong-Geol (Department of Fossil Energy and Environment Research, Korea Institute of Energy Research)
Kim, Hyun-Han (Department of Chemical and Biomolecular Engineering, KAIST)
Chang, Yong-Keun (Department of Chemical and Biomolecular Engineering, KAIST)
Publication Information
Biotechnology and Bioprocess Engineering:BBE / v.10, no.6, 2005 , pp. 571-575 More about this Journal
Abstract
A simple, but effective on-line method for estimating the mycelial cell mass concentration from agitation speed data, a most readily-available process variable, has been developed for DO-stat cultures of Agaricus blazei. The dynamic change of dissolved oxygen concentration (DOC) in the initial transient period and the change in yield were considered in the development of the estimation algorithm or estimator. Parameters in the estimation algorithm were calculated from the agitation speed data at 20% of DOC. The proposed estimator could accurately predict the cell mass concentration regardless of DOC levels in the tested range of $10{\sim}40%$, showing a good extrapolation capability.
Keywords
on-line estimation; agitation speed; DO-stat culture; Agaricus blazei;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 5  (Related Records In Web of Science)
Times Cited By SCOPUS : 5
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