Biotechnology and Bioprocess Engineering:BBE

  • 제10권6호
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  • Pages.571-575
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  • 2005
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  • 1226-8372(pISSN)
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  • 1976-3816(eISSN)
한국생물공학회 (The Korean Society for Biotechnology and Bioengineering)
권호 표지

On-Line Estimation of Cell Growth from Agitation Speed in DO-Stat Culture of a Filamentous Microorganism, Agaricus blazei

  • Na, Jeong-Geol (Department of Fossil Energy and Environment Research, Korea Institute of Energy Research) ;
  • Kim, Hyun-Han (Department of Chemical and Biomolecular Engineering, KAIST) ;
  • Chang, Yong-Keun (Department of Chemical and Biomolecular Engineering, KAIST)
  • 발행 : 2005.12.31
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초록

A simple, but effective on-line method for estimating the mycelial cell mass concentration from agitation speed data, a most readily-available process variable, has been developed for DO-stat cultures of Agaricus blazei. The dynamic change of dissolved oxygen concentration (DOC) in the initial transient period and the change in yield were considered in the development of the estimation algorithm or estimator. Parameters in the estimation algorithm were calculated from the agitation speed data at 20% of DOC. The proposed estimator could accurately predict the cell mass concentration regardless of DOC levels in the tested range of $10{\sim}40%$, showing a good extrapolation capability.

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참고문헌

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