• Title/Summary/Keyword: agitation

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Effect of Aeration-Agitation on Coenzyme Q10 Production Using Rhodobacter sphaeroides

  • Jeong, Soo-Kyoung;Kim, Joong-Kyun
    • Fisheries and Aquatic Sciences
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    • v.11 no.4
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    • pp.224-228
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    • 2008
  • With the aim of increasing the $CoQ_{10}$ production in mass culture, the effect of aeration-agitation on the $CoQ_{10}$ production using Rhodobactor sphaeroides was investigated in a l-L bioreactor. The maximum $CoQ_{10}$ production was 1.69 mg/g of dry cell weight under conditions of 50 Lux, $30^{\circ}C$, 300 rpm, and 5-vvm aeration. The $CoQ_{10}$ production was improved to produce 2.91 mg/g of dry cell weight under reduced conditions of agitation speed (200 rpm) and aeration rate (0.2 vvm). When R. sphaeroides was cultivated under more reduced DO levels during the exponential phase of the cell, the $CoQ_{10}$ production yield of 3.88-mg/g dry cell weight was the maximum obtained. Therefore, poorer conditions of aeration-agitation resulted in higher production of $CoQ_{10}$, and thus DO content was a crucial factor in the production of $CoQ_{10}$. Accordingly, it was necessary to control the DO concentration in order to enhance the $CoQ_{10}$ biosynthesis within a large-scale production.

The Fabrication of Megasonic Agitated Module(MAM) for the Improved Characteristics of Wet Etching

  • Park, Tae-Gyu;Yang, Sang-Sik;Han, Dong-Chul
    • Journal of Electrical Engineering and Technology
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    • v.3 no.2
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    • pp.271-275
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    • 2008
  • The MAM(Megasonic Agitated Module) has been fabricated for improving the characteristics of wet etching. The characteristics of the MAM are investigated during the wet etching with and without megasonic agitation in this paper. The adoption of the MAM has improved the characteristics of wet etching, such as the etch rate, etch uniformity, and surface roughness. Especially, the etching uniformity on the entire wafer was less than ${\pm}1%$ in both cases of Si and glass. Generally, the initial root-mean-square roughness($R_{rms}$) of the single crystal silicon was 0.23nm. Roughnesses of 566nm and 66nm have been achieved with magnetic stirring and ultrasonic agitation, respectively, by some researchers. In this paper, the roughness of the etched Si surface is less than 60 nm. Wet etching of silicon with megasonic agitation can maintain nearly the original surface roughness during etching. The results verified that megasonic agitation is an effective way to improve etching characteristics of the etch rate, etch uniformity, and surface roughness and that the developed micromachining system is suitable for the fabrication of devices with complex structures.

Feedback Control of Dissolved Oxygen with Hardware Modification of Fermenter (발효기 하드웨어 변환을 이용한 용존산소 제어)

  • 이중헌;이인영;박영훈
    • KSBB Journal
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    • v.15 no.3
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    • pp.280-284
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    • 2000
  • The simple imcroprocessor device was provided for the control of dissolved oxygen(DO) level. The DO analog signal output from fementer was used as a measurement variable and the agitation speed of fermenter was used as a manipulatio variable. Agitation speed was manipulated to maintain DO level above 10% of saturation condition during cell growth period with microprocessor. Since the experimental resultsshowed that the DO level was maintained above 10% and agitation speed was inverse proportional to DO level ithis simple control device can be used for the DO control of fermenter.

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Effects and Batch Kinetics of Agitation and Aeration on Submerged Cultivation of Ganoderma Iucidum (영지의 액체배양에 미치는 통기.교반의 효과와 동력학적 특성)

  • 이학수;정재현;이신영
    • KSBB Journal
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    • v.16 no.3
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    • pp.307-313
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    • 2001
  • The effects of agitaion and aeration on mycelial growth, exo-polysaccharide (EPS) production, and substrate consumption upon the submerged cultivation of G. lucidum were investigated, and the batch kinetics of the EPS fermentation of G. lucidum were interpreted as function of agitation speed and aeration rate. In a 2.6 L jar fermenter system, the optimum agitation speed and aeration rate for EPS production were determined to be 400 rpm and 1.0 vvm, respectively. The maximum production of EPS obtained was 15 g/L. The logistic model for mycelial growth fitted the experimental data better than that determined by the Monod and the two-thirds power models. The Luedeking-Piret equation adequately modelled the kinetic data obtained for product and substrate.

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Effect of Ultrasonic Agitation on Pd Catalyst Treatment (파라듐 촉매화 처리에 미치는 초음파 교반의 영향)

  • 김동규;이홍로;추현식
    • Journal of the Korean institute of surface engineering
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    • v.34 no.6
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    • pp.545-552
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    • 2001
  • Effect of ultrasonic agitation on Pd catalyst treatment was studied in metallization of ceramic boards by Cu electroless plating method.96% $Al_{2}$$O_{3}$ ceramic boards were used as substrate. In this study, the ultrasonic frequency of 28kHz was applied. In Pd catalyst, high density Pd nuclei of small size were formed during ultrasonic agitation. Density of Pd was more improved when using of ultrasonic then no stirring. In electroless plating, plating rate was in the range of 0.6~1.8$\mu\textrm{m}$/hr, which value increased with Rochelle Salts addition. Adhesion strength between ceramic boards and Cu layer was improved of 20% when using ultrasonic agitation at $30^{\circ}C$ ,5min.

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Effect of agitation speed on production of exopolymer by Pseudomonas elodea NK-2000

  • Lee, Nam-Gyu;Kim, Hyeon-Suk;Son, Chang-U;Lee, Yu-Jeong;Jo, Yeong-Su;Lee, Jin-U
    • 한국생물공학회:학술대회논문집
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    • 2000.11a
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    • pp.334-337
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    • 2000
  • Extracellular polysaccharide was produced by Pseudomonas elodea NK-2000 under nitrogen limitation and aerobic condition, The effect of agiatation speed on cell growth and production of exopolymer was investigated. The agitation speed of 7.5 L fermentor ranged from 200 to 500 rpm. Production of exopolymer increased with higher agitation speed. Maximal cell growth and production of exopolymer from 2% glucose were 3.35 g/l and 3.80 g/l, respectively when agitation speed was 400 rpm.

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On-Line Estimation of Cell Growth from Agitation Speed in DO-Stat Culture of a Filamentous Microorganism, Agaricus blazei

  • Na, Jeong-Geol;Kim, Hyun-Han;Chang, Yong-Keun
    • Biotechnology and Bioprocess Engineering:BBE
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    • v.10 no.6
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    • pp.571-575
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    • 2005
  • A simple, but effective on-line method for estimating the mycelial cell mass concentration from agitation speed data, a most readily-available process variable, has been developed for DO-stat cultures of Agaricus blazei. The dynamic change of dissolved oxygen concentration (DOC) in the initial transient period and the change in yield were considered in the development of the estimation algorithm or estimator. Parameters in the estimation algorithm were calculated from the agitation speed data at 20% of DOC. The proposed estimator could accurately predict the cell mass concentration regardless of DOC levels in the tested range of $10{\sim}40%$, showing a good extrapolation capability.

A Study on the Determination of Ionic and Molecular Weight of Dissolved Substance by Dialysis Method (II). Effect of the Agitation on Dialysis (透析法에 依한 이온量 및 分子量의 決定에 關한 硏究 (第二報). 攪拌이 透析에 미치는 影響)

  • Yun Kyoung Shin;Jhun Lee
    • Journal of the Korean Chemical Society
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    • v.17 no.3
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    • pp.213-223
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    • 1973
  • This report has been focused on the studies of the hydraulic pressure distribution which is created by the agitation of the both liquids, dialysing solution and solvent, on either side of the dialysis membrane surface. Agitators of the both liquids have been investigated and developed which can counterbalance the hydraulic pressures. The effects of the agitation velocities on the dialysing velocity have been studied under the conditions which allow the uniform dialysis on the whole surface of the dialysis membrane. Each agitation velocity of the dialysing solution and solvent, at which the dialysis coefficient is obtained most precisely, has been determined.

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Effect of agitation on hydrothermal preparation of $\alpha$-$SiO_2$ powder (수열합성법에 의한 $\alpha$-$SiO_2$분말 제조시 교반의 영향)

  • 임진홍;서경원;목영일;이강인;유효신;이철경
    • Journal of the Korean Crystal Growth and Crystal Technology
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    • v.9 no.2
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    • pp.191-196
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    • 1999
  • Effects of agitation and heating rate on crystallinity, size distribution and mean size of $\alpha$-$SiO_2$powder prepared hydrothermally were investigated. $\alpha$-$SiO_2$crystalline powder, in mean particle size of 1~3.2 $\mu\textrm{m}$, was obtained at $350^{\circ}C$ using KOH as a mineralized for a 3 h reaction. Experimental results showed that particle size became smaller as the rate of agitation increased if it was introduced from the beginning of reaction, however, crystallinity was reduced at the low rate of agitation and it was became enhanced at above 150 r/min. Particle size became larger if agitation was introduced at any time during the reaction rather than introduced from the beginning of reaction. It was also found that particle size became smaller if heating rate was reduced, while the rate of agitation kept constant.

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A Study on Fluid Dynamics for Effect of Agitation Velocity on Nutrients Removal in High Rate Algae Stabilization Pond (고율 조류 안정화지에서 교반속도가 영양염류 제거에 미치는 영향에 관한 유체동역학적 연구)

  • 공석기
    • Journal of Environmental Science International
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    • v.10 no.1
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    • pp.65-71
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    • 2001
  • HRP(high rate pond) which had kept the manufactured clay of 3cm-thickness as benthic clay in reactor and the 6 flat-blade turbine as impeller for agitation was named HRASP(high rate algae stabilization pond). And the experiment for treatment of artificial synthesis wastewater containing COD :300mg/$\ell$, NH$_3$-N : 300mg/$\ell$, T-P : 9mg/$\ell$ as nutrients was been performed successfully. This reactor was been operated under conditions : 24hrs.-irradiation and water temperature, $25^{\circ}C$ and pH 7 and agitation velocity, 15, 30, 45rpm and the effect of agitation velocity on algal bioaccumulation of nutrients was been studied with view point of fluid dynamics. The next followings could be obtained as results. 1. The agitation with a turbine impeller blade in HRASP makes clay particle indicate superior suspension effect by means of forming of excellent curl/shear flow in reactor. 2. The excessive suspension of clay particle which is created at 45rpm as rotation velocity of impeller blade of turbine disturbs the light penetration and algal photosynthesis reaction. 3. Efficiencies for removal of nutrients come out as COD : 93.9%~94.3%, ($NH_3-N + NO_3-N$) : 81.9%~99.0%, T-P : 46.8%~53.6%. 4. Kuo values of $K_1$for algal growth come out seperately as 15rpm : $1.876{\times}10^{-2}, 30rpm : 4.618{\times}10^{-3}$. 5. Kuo values of $K_2$for removal of N, P come out seperately as 15rpm : $8.403{\times}10^{-1}$ and $1.397{\times}10^{-1}$, 30rpm : $4.823{\times}10^{-1} and 2.052{\times}10^{-1}$. 6. It can be guessed easily that the excessive agitation can inhibit the algal and bacterial symbiotic reaction if it is considered that micro organism\` sense to preservation of life is relied on natural function of metabolism. Therefore the studies for this matter should be followed continuously.

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