• 한국어병학회지
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• 제22권1호
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• pp.23-33
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• 2009

V. harveyi는 양식산업에서 중요한 해산 양식 어류와 새우류에 많은 폐사를 일으키는 병원체로 알려져 있다. 본 연구에서는 해산 양식어류에서 분리된 11개 strain의 Vibrio harveyi의 생화학적, 생리학적, 혈청학적, 면역학적 특성을 조사 하였다. 생화학적 특성과 TCBS agar에서 집락의 색깔이 달라 표현형의 차이가 있었고 V. harveyi 분리균주들은 편모항원에 의해 4개 이상의 혈청형으로 구분되었다. 어류에서 분리된 V. harveyi는 western blotting으로 26-34 kDa 사이에 동일한 크기의 major protein을 갖는 group A로 구분 되었고 넙치에 대하여 표준균주인 V. harveyi KCCM40866 보다 강한 병원성을 갖고 있었다. V. harveyi C05011로 면역 유도된 넙치는 다른 group A 균주를 인위감염 시켰을 때 높은 항병력을 나타내었다.

• Journal of Microbiology and Biotechnology
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• 제20권10호
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• pp.1457-1462
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• 2010

Three types of serotypically atypical Shigella flexneri isolates were collected between 2007 and 2008 from Korean patients at the Korea National Institute of Health (NIH). These atypical isolates were characterized and compared with serologically typical S. flexneri. The first grouping of 11 atypical isolates displayed agglutination only with polyB antiserum and exhibited no reaction with any typing or grouping sera (PolyB:un). The second group of 3 isolates displayed reactions with typing sera IV, but also did not bind with any grouping sera (IV:un). The third group of 14 isolates exhibited a plural agglutination pattern, reacting with typing sera II, and two grouping sera (II:(3)4,7(8)). Amongst these atypical isolates, isolates belonging to IV:un and II:(3)4,7(8) exhibited greater antibiotic resistance, in particular to ampicillin, streptomycin, and trimethoprim-sulfamethoxazole, than typical S. flexneri strains. Furthermore, all II:(3)4,7(8) strains harbored integrons. This study suggests that these multiple antibiotic-resistant atypical S. flexneri are new subserotypes of S. flexneri that await further serological classification.

• 한국동물위생학회지
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• 제37권1호
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• pp.45-49
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• 2014

Pullorum disease and fowl typhoid are septicemic avian diseases transmitting through egg by transovarian infections. A series of tests has been performing in breeding flocks of chickens and test plans for proper inspection have been modified by government veterinary institute to control of such diseases. To improve inspection plans, different test methods were compared using fowl typhoid positive samples from a poultry farm located in Jeonbuk state in 2012. Based on first inspection, 11 samples among total 200 samples were positive by rapid slide agglutination (RSA) test and 7 samples among RSA positive samples were finally diagnosed as Salmonella Gallinarum infection by ELISA, bacterial isolation, PCR, and histopathologic examination. In the second inspection, 20 samples among total 100 samples were positive by RSA test. Among RSA positive ones, 19 samples were positive by ELISA, S. Gallinarm were successfully isolated in 3 samples, and 16 samples were positive by PCR in the cecal tonsils where were not successful for bacterial isolation. Based on histopathologic examination, severe inflammation in the 13 cecal tonsils and infiltration of lymphocytes and heterophils in the 11 livers were observed. Therefore, we suggest that bacterial isolation, PCR, and histopathologic examination methods in the third inspection need to be further used in various tissues for correct diagnosis and for final eradication of pullorum disease and fowl typhoid in breeding flocks of chickens.

• 한국응용곤충학회지
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• 제22권4호
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• pp.293-299
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• 1983

강남콩 렉틴과 근류균(R. phaseoli)의 결합에 관한 광범위한 실험을 수행하여 다음과 같은 결과를 얻었다. 1. 강남콩종자의 자엽에서 가장 많은 렉틴은 성장에 따라 뿌리로 이동하였는데 발아후 약 5일경에, 뿌리의 길이가 $6\~8cm$인 것이 가장 렉틴의 함량이 많았다. 2. 근류균의 배양시기에 따가 강남콩렉틴과의 응집력을 측정 한 결과 배양초기 가 후기보다 응집력이 높았다. 3. 강낭콩종자에 존재하는 렉틴을 추출 정제하여서 6종 근류균과의 응집력을 측정한 결과 R. phaseoli와의 응집이 가장 강했고 이는 렉틴에 대한 특이적 결합, 즉 숙주특이성을 입증하는 것이었다. 4. 단당류에 의한 응집반응의 경쟁적 저해를 시도함으로써 강남콩렉틴의 근류균에 대한 결합부위가 mannose와 galactose를 포함한 oligosaccharides가는 것을 알수 있었으며 이들의 저해효과에 필요한 당의 최소농도는 6.25mM로서 측정되었다. 5. R. phaseoli와 강낭콩 렉틴을 강낭콩 뿌리에 인공감염시켜본 결과 렉틴의 경쟁적 저해가 확인되었으므로 뿌리혹형성에 렉틴의 가교가 관여한다는 가설을 입증할 수 있었다. 6. Immunodiffusion에 의한 R. phaseoli의 항원 결정기는 R. japonicum과 일부 관련성이 있을뿐 다른 Rhizobium spp.과는 전혀 다른 것으로 나타났다.

• 한국식품영양과학회지
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• 제24권6호
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• pp.837-842
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• 1995

고추가루 첨가 식이가 체액성 면역기능에 미치는 영향을 조사하였다. 실험동무로 흰쥐를 사용하여 대조군과 2%, 5%, 10% 고추가루 첨가 식이군을 설정한 후 27일간 실험식이로 사육하였다. 실험종료 10일 전에 각군의 흰쥐를 면양적혈구로 세차례 면역시킨 후 항체생성 세포수 및 혈청 중 항체 농도 등 체액성 면역기능을 중심으로 조사하였다. 그 결과, 대조군에 비해 고추가루 첨가 식이군의 항체 생성 세포수, 적혈구 응집반응 및 혈청중 항체 농도가 높은 것으로 나타났으며, 또한 이때 고추가루 첨가식이군의 각 장기조직 중 AsA 함량은 대조군에 비해 높은 것으로 나타났다. 이들 결과로 보아, 고추성분은 체액성 면역기능의 활성화에 기여하는 물질을 함유하고 있을 뿐만이 아니라, AsA의 공급효과가 뛰어난 것이 확인되었다.

• 한국수정란이식학회지
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• 제28권4호
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• pp.337-342
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• 2013

Salmonellosis is one of the life-threating diseases of goat in Bangladesh. Therefore, the present study was designed to study the prevalence of Salmonellosis, and isolation and characterizations of the Salmonella spp. from apparently healthy and diarrheic goat. A total of 47 faces samples were collected from selected place and cultured onto different prescribed medium to isolate it. In this study, 12.76% (6/47) samples were found to be positive for Salmonella spp. During culture on SS agar medium, all of the Salmonella isolates produced round, smooth, opaque, translucent and black color colonies on SS agar media. All of the isolated Salmonella spp. fermented dextrose, maltose and mannitol with production of acid and gas but did not ferment sucrose and lactose. However, these isolates had showed Indole and Voges-Proskauer test negative, Methyl-Red test positive. All of these isolates were subjected to rapid plate agglutination test with polyvalent "O" (Poly 'O') and polyvalent "H" (poly 'H') antisera where positive agglutination were observed. They were highly sensitive to ciprofloxacin, spiramycin and gentamycin; moderately sensitive to oxytetracyline, streptomycin and amoxicillin; less sensitive to sulphamethoxazole and resistant to penicillin-G. Based on the present findings, it may be concluded that the investigated Salmonella spp. from goats might be S. typhimurium, S. enteritidis, S. brandenburg, S. salford, S. newbrunswick, S. newport or S. dublin. Further study will be needed, therefore it requires further characterization using other serological and molecular techniques.

• 한국작물학회지
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• 제34권2호
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• pp.120-126
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• 1989

본 실험은 대두(Glycine max L.) 방사품종의 종자단백질에서 렉틴을 분리하여 그 특성을 조사하기 위하여 수행되었다. 렉틴의 분리방법은 (NH$_4$)$_2$SO$_4$의 50~80% 포화침전단백질을 Carboxymethyl cellulose column과 Sephadex G 100 column을 거쳐서 분리, 정제되었다. 순도의 검정은 2~30% polyacrylamide porosity gradient gel을 사용한 전기영동법으로 단일 band를 확인하였고 또한 분자량은 11% sodium dodecyl sulfate polyacrylamide gel에 의하여 134,000 dalton임을 추정하였으며 45,000 dalton의 3개의 subunits로 구성되어 있음을 알았다. Schiff reagent에 의하여 분홍색 반응을 일으켰으므로 이것은 당단백질임을 알 수 있었다. 본 당단백질은 토끼와 사람의 적혈구 모두에서 적혈구응집 반응이 일어났으며 사람의 혈액형중에서 적형구응집 정도는 A>B>O>AB형의 순서로 응집이 잘 일어났다. 당류에 의한 적혈구응집저해는 N -acetyl-D-galactoseamine 과 D-galactose에 의하여 일어났다.

• 대한수의학회지
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• 제11권1호
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• pp.41-47
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• 1971

Investigation of Leptospira icterohaemorrhagiae and Leptospira canicola in dogs was carried out from May, 1970 to March, 1971 in Korea. A total of 943 random samples from 18 animal hospitals in 9 cities were collected and sent to Taipei and tested by the rapid microscopic agglutination test giving the following results. 1. The 943 samples gave 141 positives (14.95%). 2. Among the 18 animal hospitals a total 14 positives were found, with 30.0% being the highest and 9.1% the lowest reaction, comparing each hospital. 3. Monthly positive comparison gave the highest reaction for December (31.3%) the lowest August (5.8%). Seasonal comparison June, July and August was the lowest of all four seasons. 4. Of all the 913 cases examined, males were 505 (55.3%) and females 408 (44.7%), which gives only a difference of about 10% on the basis of sex. Comparing positive reaction cases on the basis of sex gave a large difference, of almost twice. Male${\cdots}$92: Female${\cdots}$47 5. Comparing 436 (46.9%) sick dogs against 492 (53.1%) healthy dogs gave 13.3 and 16.1% positive respectively. Thus indicating that some of the healthy dogs have inapparent infection. 6. Comparing on the basis of positive serum type L. icterohaemorrhagiae gave 133 (54.7%) and L. canicola 110 (45.3%) Generally both L. icterohaemorrhagiae and L. canicola produce common reaction with positive serum. 7. Comparing by age a total of 89 positive cases gave the following results. less than 1 year${\cdots}$49 (55.5%) from 1 year to 2 years${\cdots}$26 (29.2%) from 2 year to 3 years${\cdots}$12 (13.5%) over 3 years${\cdots}$5 8. Among 80 field rats collected at Suwon and tested by rapid microscopic agglutination test for L. icterohaemorrhagiae and L. canicola gave all negative reactions.

• Parasites, Hosts and Diseases
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• 제46권3호
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• pp.199-201
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• 2008

Toxoplasma gondii is an obligate intracellular zoonotic protozoan with a worldwide distribution. It infects humans as well as a broad spectrum of vertebrate hosts. Cats and wild felidae play crucial roles in the epidemiology of toxoplasmosis. This study was performed to survey the prevalence of T. gondii infection among stray cats in the Gyeonggi-do, Republic of Korea. A total of 174 stray cat blood samples were collected from Gwacheon-si (n = 20), Bucheon-si (82), and Yangju-si (72). Positive sera for T. gondii were identified in 14 samples (8.1%) exclusively via the latex agglutination test, 28 (16.1%) via ELISA, and 23 (13.2%) via PCR analysis. The overall infection rate of female stray cats (29.2%) presented as higher than that of male cats (24.0%). This study suggests that T. gondii is widespread in the stray cat population of Gyeonggi-do, Korea. It is urgently needed to control urban stray cat population and to reduce the risk of zoonotic transmission of toxoplasmosis to other animal hosts and humans.

• 대한의용생체공학회:의공학회지
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• 제39권6호
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• pp.250-255
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• 2018

Accurate blood typing is the crucial factor for safe and successful blood transfusion and plays a very important role in organ transplantation and genetic information of forensic medicine. Microfluidic devices have been developed to overcome the limitations of the conventional blood typing methods. In this study, we demonstrate a Lamb wave-based device for simple blood typing in a sample droplet and we propose new indices for quantitative and accurate blood typing. Using Lamb wave-induced acoustic streaming in the droplet, the blood sample and the reagent can be mixed rapidly and red blood cells start to form clumps, which is agglutination. Based on the recorded image and video, the intensity of transmitted light through the sample droplet is evaluated to determine the blood type. Effect of the concentration of suspended red blood cells was evaluated and we found that 10% concentration of suspended red blood cells was suitable to observe the difference between aggregated and non-aggregated samples. Finally, sample with blood type A could be determined using anti-A reagent in our Lamb wave-based device. Our device enables simple and accurate blood typing, which can be applied to resource-limited environments.