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Suggestions for a better inspection method according to the occurrence of fowl typhoid in broiler breeders

육용종계 가금티프스 발생에 따른 검사방법 개선 방안

  • Chu, Keum-Suk (Iksan-Branch, Jeonbuk Institute of Livestock & Veterinary Research) ;
  • Kim, Kyoung-Taek (College of Veterinary Medicine, Chonbuk National University) ;
  • Yoon, Eun-Jeong (Iksan-Branch, Jeonbuk Institute of Livestock & Veterinary Research) ;
  • Kim, Beom-Seok (College of Veterinary Medicine, Chonbuk National University)
  • 추금숙 (전라북도축산위생연구소 익산지소) ;
  • 김경택 (전북대학교 수의과대학) ;
  • 윤은정 (전라북도축산위생연구소 익산지소) ;
  • 김범석 (전북대학교 수의과대학)
  • Received : 2013.11.20
  • Accepted : 2014.01.20
  • Published : 2014.03.30

Abstract

Pullorum disease and fowl typhoid are septicemic avian diseases transmitting through egg by transovarian infections. A series of tests has been performing in breeding flocks of chickens and test plans for proper inspection have been modified by government veterinary institute to control of such diseases. To improve inspection plans, different test methods were compared using fowl typhoid positive samples from a poultry farm located in Jeonbuk state in 2012. Based on first inspection, 11 samples among total 200 samples were positive by rapid slide agglutination (RSA) test and 7 samples among RSA positive samples were finally diagnosed as Salmonella Gallinarum infection by ELISA, bacterial isolation, PCR, and histopathologic examination. In the second inspection, 20 samples among total 100 samples were positive by RSA test. Among RSA positive ones, 19 samples were positive by ELISA, S. Gallinarm were successfully isolated in 3 samples, and 16 samples were positive by PCR in the cecal tonsils where were not successful for bacterial isolation. Based on histopathologic examination, severe inflammation in the 13 cecal tonsils and infiltration of lymphocytes and heterophils in the 11 livers were observed. Therefore, we suggest that bacterial isolation, PCR, and histopathologic examination methods in the third inspection need to be further used in various tissues for correct diagnosis and for final eradication of pullorum disease and fowl typhoid in breeding flocks of chickens.

Keywords

References

  1. 농림수산검역검사본부. 2011. 가축질병병성감정실시요령. 농림수산검역검사본부 고시 제2011-2호.
  2. 농림수산부. 2004. 종계장 부화장 방역관리요령. 농림부고시 제 2004-74호.
  3. 농림수산식품부. 2011. 종계장.부화장 방역관리요령. 농림수 산식품고시 제2011-196호.
  4. Choi YJ, Kim DK, Kim YH. 2000. Epidemiological characteristics on fowl typhoid outbreak in Kyungnam province and comparison of diagnostic method for identification of Salmonella Gallinarum. Korean J Vet Serv 23: 349-360.
  5. Gast RK, Shivaprasad HL. 2003. Salmonella infections. pp. 567-613. In: Saif YM(Ed). Diseases of poultry. 11th ed. Iowa State University Press, Ames, lowa.
  6. Kang MS, Kwon YK, Jung BY, Kim A, Lee KM, An BK, Song EA, Kwon JH, Chung GS. 2011. Differential identification of Salmonella enterica subsp. enterica serovar Gallinarum biovars Gallinarum and Pullorum based on polymorphic regions of glgC and specC genes. Vet Microbiol 147: 181-185. https://doi.org/10.1016/j.vetmic.2010.05.039
  7. Kang SS, Park JM, Lee JJ, Yuk MJ, Byeon CS, Seo HW, Choi HY. 2003 The study on the characteristics of fowl typhoid and eradication program. Korean J Vet Serv 26: 135-144.
  8. Kim HH, Park MS, Kang YH, Kim SH, Yu JY, Chung BK, Lee BK. 1998. Epidemiological characteristics of Salmonella strains isolated in Korea in 1997. Kor J Vet Publ Hlth 22: 253-260.
  9. Kim JT, Shim HS, Kim TJ, Ko TO, Woo JT, Ryu KS, Park YS. 1998. Studies on serological tests for pullorum disease. Korean J Vet Serv 21: 313-323.
  10. Kim YS, Kim SK, Song WC, Hwang EK. 2001. Comparison of polymorphisms of Salmonella Pullorum and Salmonella Gallinarum using DNA amplified fingerprinting. Korean J Vet Res 41: 357-365.
  11. Lee DS, Hahn TW. 2000. Virulence, antibiotics susceptibility and plasmid profiles of Salmonella Gallinarum isolated from chickens in Korea. Kor J Vet Publ Hlth 24: 49-57.
  12. Lee YJ, Kim KS, Kim YK, Kwon YK, Tak RB. 2003. Biochemical characteristics and antimicrobials susceptibility of Salmonella gallinarum isolated in Korea. J Vet Sci 4: 161-166.
  13. Oh GH, Choi WP. 1994. Studies on Salmonella isolated from chicks. Korean J Vet Res 34: 501-510.
  14. Park KY, Yeh JG, Park SG. 1994. Characteristics of Salmonella species isolated from poultry. Kor J Vet Publ Hlth 18: 107-116.
  15. Park MK, Choi KS, Kim MC, Chae JS. 2001. Differential diagnosis of Salmonella Gallinarum and S Pullorum using PCR-RFLP. J Vet Sci 2: 213-219.
  16. Pak SI, Park CK. 2008. Determination of sample size to serological surveillance plan for pullorum disease and fowl typhoid. Korean J Vet Res 48: 457-462.
  17. Proux K, Humbert F, Jouy E, Houdayer C, Lalande F, Oger A, Salvat G. 2002. Improvements required for the detection of Salmonella Pullorum and Gallinarum. Can J Vet Res 66: 151-157.
  18. Ryu JY, Jun MH, Chang KS, Son HS, Kwak HK, Park KJ, Woo YK. 1999. Isolation of Salmonella from the layer chickens reacting in pullorum-typhoid agglutination test. Korean J Vet Serv 22: 221-237.
  19. Shah DH, Park JH, Cho MR, Kim MC, Chae JS. 2005. Allele-specific PCR method based on rfbS sequence for distinguishing Salmonella Gallinarum from Salmonella Pullorum : serotype-sepecific rfbS sequence polymorphism. J Microbiol Methods 60: 169-177. https://doi.org/10.1016/j.mimet.2004.09.005