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Serological characteristics and antigenicities of Vibrio harveyi isolated from marine cultured fish  

Oh, Yun-Kyeong (Pathology Division, National Fisheries Research and Development Institute)
Kim, Myoung-Sug (Pathology Division, National Fisheries Research and Development Institute)
Park, Myoung-Ae (Pathology Division, National Fisheries Research and Development Institute)
Kim, Jin-Woo (Pathology Division, National Fisheries Research and Development Institute)
Cho, Ji-Young (Deptartment of Marine biotechnology, Soonchunhyang University)
Jeong, Hyun-Do (Department of Aquatic Life Medicine, Pukyong National University)
Publication Information
Journal of fish pathology / v.22, no.1, 2009 , pp. 23-33 More about this Journal
Abstract
Vibrio harveyi was a significant pathogenic agent and cause a high mortality of cultured fish and shrimp in the aquaculture industry. In this study, we have investigated biochemical, physiological, serological and immunological characteristics of V. harveyi isolated from marine cultured fish. The phenotypes of V. harveyi were differentiated with their own biochemical characteristics and colors of colony on the TCBS agar. V. harveyi were classified into more than four serogroups by agglutination test. Most isolates were classified into a group A which is categorized with the same band pattern generated by western blotting. Group A was characterized by a major protein, which is ranged from 26 and 34 kDa in size, and has a virulence to oliver flounder more than reference strain KCCM40866. Oliver flounders vaccined with FKC of V. harveyi C05011 were highly resistant to infection by other strains of group A.
Keywords
Vibrio harveyi; Classification; Antigenic protein; Agglutination; Western blotting;
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