• 한국식품영양과학회지
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• 제33권10호
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• pp.1715-1719
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• 2004

항균성이 있는 것으로 알려진 자몽종자추출물에 젖산과 구연산을 각각 5%씩 첨가한 식물성 천연항균제 제재와 제올라이트를 첨가하여 항균포장지를 만든 다음 감귤과 배의 저장을 위한 포장재로로 사용하였다. 저장 기간중 품질 변화의 지표로 중량감소율, pH 산도, 가용성고형 물, 총균수, 효모 및 곰팡이수 등을 측정하였다. 감귤은 저장 2주가지 대조구와 항균제 6% 첨가 포장지 시험구의 중량 감소가 대체적으로 큰 것으로 나타났으나 그 이후에는 항균제 9$^{\circ}C$ 첨가 포장지 시험구의 중량 감소가 큰 것으로 나타났다. 포장직후 감귤의 pH는 3.77이 었으며, 저장 기간이 경과함에 따라 대체적으로 증가하였으며, 총산은 pH의 증가와는 반대로 대체적으로 감소하였다. 감귤의 저장전 총균수는 1.07 H $10^{5}$ CFW/g이었으며, 효모 및 곰팡이 는 4.9 H $10^4$ CFU/g이었다. 즉 감귤의 표피에 있는 미생물의 대부분은 효모와 곰팡이였으며, 저장 2주까지는 항균제 5% 첨가 포장지에서는 대조구와 비슷하거나 다소 높은 미생물수를 나타내었으나 다른 처리구는 대조구보다 낮아서 항균 효과가 있는 것으로 나타났다. 포장지에 항균제의 첨가 비율이 높을수록 감귤의 부패율은 낮았으며, 저장 4주에 항균제 12% 첨가 포장지로 포장한 시험구는 18.0%인데 비하여, 대조구는 34.7%로 나타났다. 배는 항균제 9% 및 12% 첨가 포장지로 포장한 시험구의 총균수, 효모 및 곰팡이수가 저장 2주까지는 다른 시험구보다 낮았으며, 12% 첨가 포장지의 효과가 더 큰 것으로 나타났다. 저장 2주 이후에는 항균제 9%, 12% 첨가 포장지 모두 미생물수가 급격하게 증가한 것은 항균포장지에 의한 배의 미생물 생육 억제 효과가 제한적일 수 있음을 나타내는 것이라고 판단된다. 따라서 다른 품질특성과 미생물수의 변화를 동시에 고찰하면 항균포장지에 의한 배의 저장은 2∼3주 이내로 제한적일 수 있을 것으로 보인다.다.

• 한국환경보건학회지
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• 제39권4호
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• pp.312-321
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• 2013

Objective: In May 2011, an interview with three United States Forces Korea veterans revealed that chemicals believed to be 'Agent Orange' were buried at Camp Carroll (situated in Waegwan, Chilgok-gun, Gyeongsangbuk-do, Korea) in 1978. Many hazardous chemicals, such as perchloroethylene (PCE), trichloroethylene (TCE), and organochlorines were subsequently found in a joint US-ROK environmental investigation, although dioxins or burial evidence related to 'Agent Orange' have been not uncovered. This study was carried out to investigate the relevance of hazardous chemical exposure and health effects of the residents around Camp Carroll. Methods: The authors conducted a health questionnaire survey among residents around Camp Carroll regarding Waegwan groundwater ingestion, length of residence in Waegwan, and physician-diagnosed disease history (such as cancer, hypertension, diabetes and etc.). Logistic regression was performed to identify the associations between hazardous chemical exposure and physician-diagnosed diseases. Results: Among the 5,320 residents, excluding those living outside the Waegwan area, 3,430 subjects age 30 and over were analyzed. Among females, upon correction of age, smoking history and Camp Carroll working history, a higher distribution in the Waegwan groundwater ingestion group was demonstrated for patients with physician-diagnosed cancer and hypertension. The odds ratios have a tendency to increase with increased length of Waegwan groundwater ingestion. Conclusions: This study may be limited by not adopting a cohort study for the evaluation of factors that may confound environmental exposure. Yet it is meaningful that the correlation between Waegwan groundwater ingestion and chronic diseases were found through regression analysis in female; and further, this outcome may be used as a sampling basis for a secondary epidemiologic study.

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 2001년도 제53차 추계 학술대회 연제집
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• pp.418-419
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• 2001

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 2001년도 제53차 추계 학술대회 연제집
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• pp.416-417
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• 2001

• 대한예방의학회:학술대회논문집
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• 대한예방의학회 1999년도 제51차 추계 학술대회 연제집
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• pp.144-145
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• 1999

• 한국발생생물학회지:발생과생식
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• 제18권4호
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• pp.225-231
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• 2014

Autophagy is a homeostatic degradation process that is involved in tumor development and normal development. Autophagy is induced in cancer cells in response to chemotherapeutic agents, and inhibition of autophagy results in enhanced cancer cell death or survival. Chloroquine (CQ), an anti-malarial drug, is a lysosomotropic agent and is currently used as a potential anticancer agent as well as an autophagy inhibitor. Here, we evaluate the characteristics of these dual activities of CQ using human colorectal cancer cell line HCT15. The results show that CQ inhibited cell viability in dose- and time-dependent manner in the range between 20 to 80 uM, while CQ did not show any antiproliferative activity at 5 and 10 uM. Cotreatment of CQ with antitumor agent NVP-BEZ235, a dual inhibitor of PI3K/mTOR, rescued the cell viability at low concentrations meaning that CQ acted as an autophagy inhibitor, but CQ induced the lethal effect at high concentrations. Acridine orange staining revealed that CQ at high doses induced lysosomal membrane permeabilization (LMP). High doses of CQ produced cellular reactive oxygen species (ROS) and cotreatment of antioxidants, such as NAC and trolox, with high doses of CQ rescued the cell viability. These results suggest that CQ may exert its dual activities, as autophagy inhibitor or LMP inducer, in concentration-dependent manner.

• 생명과학회지
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• 제12권2호
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• pp.182-187
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• 2002

본 연구에서는 알킬화제인 methylmethane sulfonate (MMS)를 선처리한 NIH3T3세포에서 소목추출물의 효과를 분석하였다. MTT 분석결과 MMS에 의해서 유도된 세포생존률이 소목추출물에 의해서 감소되었다. 세포형태분석, acridine orange 염색법, 그리고 DNA fragmentation 분석에서 MMS에 의해서 유도된 세포고사의 특징인 핵 응축 및 DNA laddering이 소목추출물에 의해서 증가됨이 관찰되었다. 이러한 결과들로 소목추출물은 NIB73T3 세포에서 MMS에 의해서 유도된 세포고사를 촉진시킴을 보여준다.

• Membrane and Water Treatment
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• 제10권5호
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• pp.361-372
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• 2019

Effect of different alkane based solvents on the stability of emulsion liquid membrane was investigated using normal alkanes (n-hexane, n-heptane, n-octane and n-decane) under various operating parameters of surfactant concentration, emulsification time, internal phase concentration, volume ratio of internal phase to organic phase, volume ratio of emulsion phase to external phase and stirring speed. Results of stability revealed that emulsion liquid membrane containing n-octane as solvent and span-80 (5 % (w/w)) as emulsifying agent presented the highest amount of emulsion stability (the lowest breakage) compared with other solvents; however, operating parameters (surfactant concentration (5% (w/w)), emulsification time (6 min), internal phase concentration (0.05 M), volume ratio of internal phase to organic phase (1/1), volume ratio of emulsion phase to external phase (1/5) and stirring speed (300 rpm)) were also influential on improving the stability (about 0.2% breakage) and on achieving the most stable emulsion. The membrane with the highest stability was employed to extract acridine orange with various concentrations (10, 20 and 40 ppm) from water. The emulsion liquid membrane prepared with n-octane as the best solvent almost removed 99.5% of acridine orange from water. Also, the prepared liquid membrane eliminated completely (100%) other cationic dyes (methylene blue, methyl violet and crystal violet) from water demonstrating the efficacy of prepared emulsion liquid membrane in treatment of dye polluted waters.

• 대한영양사협회학술지
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• 제30권1호
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• pp.11-28
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• 2024

This study aimed to establish the optimal amount of thickening agent for the appropriate viscosity in soups and beverages, which are part of the dysphagia diet. The soups were bean sprout soup and soybean paste soup; the beverages were orange juice, regular milk, and low-fat milk; the thickening agent was one type of xanthan gum product. After adding the thickening agents (from 1 g to 5 g per 200 mL of the test food), syringe tests were conducted over time (5, 10 and 15 minutes) to verify the effects of the amount of thickening agent added per sample and the time between addition and achieving the resulting viscosity, and to establish the optimal addition conditions to reach IDDSI levels 1, 2, and 3 of the dysphagia diet. Water (based on 200 mL) was used as the standard control. These results provide a useful basis for customized diets based on the patient's dysphagia severity. On the other hand, this study is limited by including only liquid foods in the dysphagia diet and one type of xanthan gum-based thickening agent. Therefore, it is necessary to conduct continuous research, based on the study results, to modify the viscosity of the dysphagia diet using various thickening agents and foods and prevent nutritional deficiencies by managing the diet according to the patient's swallowing ability.

• 한국응용과학기술학회지
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• 제29권4호
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• pp.610-617
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• 2012

탱자에는 여러 종류의 monoterpenes, limonoids, flavonoids과 coumarins 등의 구성성분으로 이루어져 있다. 탱자의 구성 성분중에 7-제라닐옥시쿠마린는 7개의 탄소로 구성된 제라닐옥시기의 곁사슬을 가진 물질이다. 그리고 7-제라닐옥시쿠마린은 여러 약리적인 효과들을 나타낸다고 알려져 있다. 본 연구에서는 탱자의 구성성분인 7-제라닐옥시쿠마린과 이들의 다양한 유도체를 합성하였다. 그리고 항염증에 대한 항염증 효능을 알아보기 위하여 염증을 유발하는 일산화질소 억제 cytokine을 측정한 결과는 6-제라닐옥시쿠마린의 성분이 사이토카인인 인터루킨-6가 $1{\mu}M$농도에서 68.9% 를, $10{\mu}M$에서 72.6% 의 저해효과를 나타냈다.