• The Korea Journal of Herbology
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• v.24 no.4
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• pp.77-86
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• 2009

Objectives : The proliferation and migration of human aortic smooth muscle cells (HASMC) in response to activation by various stimuli plays a critical role in the initiation and development of atherosclerosis. This study was conducted to examine the effects of Nelumbo nucifera leaves (NNL) on the proliferation and migration of HASMC. Additionally, the mechanisms involved in any observed effects were also evaluated. Methods : Apoptotic cells were measured by staining with FITC-labeled annexin V, followed by flow cytometric analysis. The expression level of apoptosis related proteins was confirmed by western blot. And MMP-9 activity was measured by gelatin zymography and MMP-9 expression was measured by ELISA Results : NNL completely inhibited the proliferation of HASMC via induction of the expression of apoptotic proteins including annexin V, cleaved poly ADP-ribose polymerase (PARP), and caspase-3 and -8. NNL treatment resulted in the release of cytochrome c into cytosol, a loss of mitochondrial membrane potential, a decrease in Bcl-2 and Bcl-xL and an increase in Bax expression. NNL also blocked HASMC migration via suppression of MMP-9. Conclusions : Taken together, these results indicate that NNL has the potential for use as an anti-artherosclerosis agent.

• Journal of Adhesion and Interface
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• v.12 no.4
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• pp.144-150
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• 2011

The effect of low profile agent and release agent on the surface and mechanical properties of bulk mold compound were investigated. Atomic content and contact angle of surface were characterized using X-ray photoelectron spectroscopy and contact anglemeter. Surface morphology and surface roughness were obtained using field emission scanning electron microscope and atomic force microscope, respectively. As increasing the low profile agent from 0 to 9.2 wt%, the volume shrinkage and surface roughness decreased from 0.35% to 0.05%, and from $0.27{\mu}m$ to $0.12{\mu}m$, respectively. The increase of release agent from 1.8 wt% to 3.6 wt% resulted in the migration of release agent to sample surface and it increased the surface roughness. The flexural strength and impact strength were decreased approximately 30% as the low profile agent increasing from 5.0 wt% to 9.0 wt%.

• Asian Pacific Journal of Cancer Prevention
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• v.13 no.9
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• pp.4815-4822
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• 2012

Invasion and metastasis are the major causes of cancer-related death. Pharmacological or therapeutic interventions such as chemoprevention of the progression stages of neoplastic development could result in substantial reduction in the incidence of cancer mortality. (-)-Epigallocatechin-3-gallate (EGCG), a promising chemopreventive agent, has attracted extensive interest for cancer therapy utilizing its antioxidant, anti-proliferative and inhibitory effects on angiogenesis and tumor cell invasion. In this study, we assessed the influence of EGCG on the proliferative potential of HeLa cells by cell viability assay and authenticated the results by nuclear morphological examination, DNA laddering assay and cell cycle analysis. Further we analyzed the anti-invasive properties of EGCG by wound migration assay and gene expression of MMP-9 and TIMP-1 in HeLa cells. Our results indicated that EGCG induced growth inhibition of HeLa cells in a dose- and time-dependent manner. It was observed that cell death mediated by EGCG was through apoptosis. Interestingly, EGCG effectively inhibited invasion and migration of HeLa cells and modulated the expression of related genes (MMP-9 and TIMP-1). These results indicate that EGCG may effectively suppress promotion and progression stages of cervical cancer development.

• Asian Pacific Journal of Cancer Prevention
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• v.15 no.15
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• pp.6269-6273
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• 2014

Growing evidence suggests that miR-150-5p has an important role in regulating genesis of various types of cancer. However, the roles and the underlying mechanisms of miR-150-5p in development of colorectal cancer (CRC) remain largely unknown. Transwell chambers were used to analyze effects on cell migration and invasion by miR-150-5p. Quantitative real-time PCR (qRT-PCR), Western blotting and dual-luciferase 3' UTR reporter assay were carried out to identify the target genes of miR-150-5p. In our research, miR-150-5p suppressed CRC cell migration and invasion, and MUC4 was identified as a direct target gene. Its effects were partly blocked by re-expression of MUC4. In conclusiomn, miR-150-5p may suppress CRC metastasis through directly targeting MUC4, highlighting its potential as a novel agent for the treatment of CRC metastasis.

• Proceedings of the Korean Environmental Sciences Society Conference
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• 2020.10a
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• pp.221-221
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• 2020

Curcumin, a hydrophobic polyphenol derived from turmeric, has been used a food additive and as a herbal medicine for the treatment of various diseases. In the present study, we found the functional role of a nanosphere loaded with curcumin (CN) in the promotion of the motility of human umbilical cord blood derived mesenchymal stem cells (hUCB-MSCs) during the wound closure. We found that the efficacy of hUCB-MSCs migration induced by CN was 1000-fold higher than that of curcumin powder. CN significantly increased the motility of hUCB-MSCs by activating c-Src, which is responsible for the phosphorylation of protein kinase C (PKC) and extracellular signal-regulated kinase (ERK). CN induced the expression levels of α-actinin-1, profilin-1 and filamentous-actin, as regulated by the phosphorylation of nuclear factor-kappa B during its promotion of cell migration. In a mouse skin excisional wound model, we found that transplantation of UCB-MSCs pre-treated with CN enhances wound closure, granulation, and re-epithelialization at mouse skin wound sites. These results indicate that CN is a functional agent that promotes the mobilization of UCB-MSCs for cutaneous wound repair.

• Annual Conference of KIPS
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• 2006.11a
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• pp.551-554
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• 2006

Java 기반의 이동 에이전트 시스템은 에이전트의 실행 상태를 이주시킬 수 없는 문제점을 가지고 있다. 이러한 문제점을 해결하기 위해 JVM을 수정하거나 이주에 필요한 소스 코드, 혹은 바이트 코드를 삽입하는 메커니즘을 이용한 이동 에이전트 시스템들이 연구되었다. 하지만 이러한 시스템들은 이식성이 떨어지거나 에이전트의 이주부분을 프로그래밍 할 수 없는 단점이 존재한다. 또한 이러한 시스템들은 플랫폼의 요청에 의한 에이전트 이주 기법인 forced migration을 지원하지 않는다. 본 논문에서는 AOP(Aspect Oriented Programming)를 이용한 에이전트의 투명한 이주 기법을 제안한다. 제안 기법에서는 에이전트를 비즈니스 로직, 이동성 코드, 상태 저장 코드로 나누어 개발하고, 이를 직조하여 이동 에이전트를 개발한다. 제안 기법을 사용하면 에이전트 개발자는 이동 에이전트의 비즈니스 로직 개발에 집중할 수 있고 에이전트의 이주 부분을 프로그래밍 함으로써, 유연한 에이전트의 이주 정책을 수립할 수 있다.

• BMB Reports
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• v.56 no.7
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• pp.410-415
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• 2023

Breast cancer has become the most common cancer among women worldwide. Among breast cancers, metastatic breast cancer is associated with the highest mortality rate. Twist1, one of the epithelial-mesenchymal transition-regulating transcription factors, is known to promote the intravasation of breast cancer cells into metastatic sites. Therefore, targeting Twist1 to develop anti-cancer drugs might be a valuable strategy. In this study, LY-290181 dose-dependently inhibited migration, invasion, and multicellular tumor spheroid invasion in breast cancer cell lines. These anti-cancer effects of LY-290181 were mediated through the down-regulation of Twist1 protein levels. LY-290181 inhibited extracellular signal-regulated kinase and c-Jun N-terminal kinase signaling pathways. Therefore, our findings suggest that LY-290181 may serve as a basis for future research and development of an anti-cancer agent targeting metastatic cancers.

• Journal of the Korea Institute of Information and Communication Engineering
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• v.12 no.2
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• pp.378-384
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• 2008

The sensor network for the health care application service has the man or movable object as the main sensing object. In order to support inter-node interaction by the movement of such sensing objects, the node's dynamic function modification, dynamic self-configuration and energy efficiency must be considered. In this paper, the Agilla model which supports the dynamic function modification through the agent migration between nodes and LEACH protocol which guarantees the dynamic self-configuration and energy efficiency through the configuration of inter-node hierarchical cluster configuration are analyzed. Based on the results of the analysis, the Mobility Agent Middleware which supports the dynamic function modification between nodes is designed, and LEACH_Mobile protocol which guarantees the node nobility as the weakness of the existing LEACH protocol is suggested. Also, the routing module which supports the LEACH_Mobile protocol is designed and the interface for conjunction with Mobility Agent Middleware is designed. Then, it is definitely increase performance which un mobility node of transfer data rate through LEACH_Mobile protocol of simulation result.

• The Journal of Korean Institute of Communications and Information Sciences
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• v.29 no.1C
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• pp.25-34
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• 2004

In mobile agent environments, cascaded delegations among places occur frequently due to the mobility of agents. Cascaded delegation in mobile agent environments can be defined as the process whereby the delegated place delegates the rights of the delegating place further. The representative study for delegation in mobile agent environments is Berkovits et al.'s study. Their study only defines the messages that is sent between the place executing the agent and the place where the agent migrates. Because their study considers only the delegation between two places which participate in migration of an agent, it is inadequate in the situation that the cascaded delegation is necessary. In other words, the relationships among the messages sent from and to places is necessary. However, their study does not exist the relationships. In this paper, we propose a delegation scheme that provides agents with secure cascaded delegation. The proposed scheme achieves the goal by nesting each delegation token within the signed part of the next immediate delegation token. We prove that the proposed scheme is secure against the attack of replaying a message and of substituting a delegation token.

• Journal of the Korea Institute of Information Security & Cryptology
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• v.13 no.6
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• pp.77-84
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• 2003

A mobile agent is a program which represents a user in a network and is capable of migrating from one node to another node, performing computations on behalf of the user. In this paper, we suggest a scheme that can safely recover mobile agent using the checkpoint that is saved at the platform that it visited previously and restart its execution from the abnormal termination point of the mobile agent. For security, mobile agent uses its public key to encrypt the checkpoint and the home platform uses the private key of the mobile agent to decrypt the encrypted checkpoints at the recovery stage. When home platform receives the checkpoint of the mobile agent, home platform verifies the checkpoint using message digest. Home platform verifies the correctness of the checkpoint by comparing the message digest generated at checkpoint mention time with the message digest generated at mobile agent recovery time.