The Korea Journal of Herbology (대한본초학회지)

The Korea Association of Herbology (대한본초학회)
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Nelumbo nucifera Leaves Inhibit HASMC Proliferation and Migration Activated by TNF-$\alpha$

Human Aortic Smooth Muscle Cell에서 하엽(荷葉)의 항동맥경화 활성 연구

  • Kim, Sun-Mo (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Yun, Hyun-Jeong (Cardiovascular Medical Research Center, College of Oriental Medicine, Dongguk University) ;
  • Yi, Hyo-Seung (Cardiovascular Medical Research Center, College of Oriental Medicine, Dongguk University) ;
  • Won, Chan-Wook (Department of Prescriptionology, Collage of Oriental Medicine, Dongguk University) ;
  • Kim, Jai-Eun (Cardiovascular Medical Research Center, College of Oriental Medicine, Dongguk University) ;
  • Park, Sun-Dong (Cardiovascular Medical Research Center, College of Oriental Medicine, Dongguk University)
  • 김선모 (동국대학교 한의과대학 방제학교실) ;
  • 윤현정 (동국대학교 한의과대학 심혈관계질환 천연물개발연구센터) ;
  • 이효승 (동국대학교 한의과대학 심혈관계질환 천연물개발연구센터) ;
  • 원찬욱 (동국대학교 한의과대학 방제학교실) ;
  • 김재은 (동국대학교 한의과대학 심혈관계질환 천연물개발연구센터) ;
  • 박선동 (동국대학교 한의과대학 심혈관계질환 천연물개발연구센터)
  • Published : 2009.12.30
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Abstract

Objectives : The proliferation and migration of human aortic smooth muscle cells (HASMC) in response to activation by various stimuli plays a critical role in the initiation and development of atherosclerosis. This study was conducted to examine the effects of Nelumbo nucifera leaves (NNL) on the proliferation and migration of HASMC. Additionally, the mechanisms involved in any observed effects were also evaluated. Methods : Apoptotic cells were measured by staining with FITC-labeled annexin V, followed by flow cytometric analysis. The expression level of apoptosis related proteins was confirmed by western blot. And MMP-9 activity was measured by gelatin zymography and MMP-9 expression was measured by ELISA Results : NNL completely inhibited the proliferation of HASMC via induction of the expression of apoptotic proteins including annexin V, cleaved poly ADP-ribose polymerase (PARP), and caspase-3 and -8. NNL treatment resulted in the release of cytochrome c into cytosol, a loss of mitochondrial membrane potential, a decrease in Bcl-2 and Bcl-xL and an increase in Bax expression. NNL also blocked HASMC migration via suppression of MMP-9. Conclusions : Taken together, these results indicate that NNL has the potential for use as an anti-artherosclerosis agent.

Keywords

References

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