• Journal of Microbiology and Biotechnology
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• 제31권12호
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• pp.1732-1740
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• 2021

Tobacco etch virus protease (TEVp) is a useful tool for removing fusion tags, but wild-type TEVp is less stable under oxidized redox state. In this work, we introduced and combined C19S, C110S and C130S into TEVp variants containing T17S, L56V, N68D, I77V and S135G to improve protein solubility, and S219V to inhibit self-proteolysis. The solubility and cleavage activity of the constructed variants in Escherichia coli strains including BL21(DE3), BL21(DE3)pLys, Rossetta(DE3) and Origami(DE3) under the same induction conditions were analyzed and compared. The desirable soluble amounts, activity, and oxidative stability were identified to be reluctantly favored in the TEVp. Unlike C19S, C110S and C130S hardly impacted on decreasing protein solubility in the BL21(DE3), but they contributed to improved tolerance to the oxidative redox state in vivo and in vitro. After two fusion proteins were cleaved by purified TEVp protein containing double mutations under the oxidized redox state, the refolded disulfide-rich bovine enterokinase catalytic domain or maize peroxidase with enhanced yields were released from the regenerated amorphous cellulose via affinity absorption of the cellulose-binding module as the affinity tag.

• 생약학회지
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• 제39권4호
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• pp.310-315
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• 2008

Viscum album coloratum (Korean mistletoe; KM) is a semi-parasitic plant growing on various trees. This plant hasbeen shown to possess a variety of biological functions such as immunomodulation, apoptosis-induction and antitumor activity. However, there is no information about how the extract of KM affects life span of the host. In this study, in order to examine the effect of KM on the longevity of the host, we investigated whether a Korean mistletoe extract (KME) was able to extend the life length in an experimental model using the spring silkworm moth of Bombyx mori (Lepidoptera bombycidae). We established a model test for survival in which all of the male and female moths died within 18 days after the beginning of the experiment. Mean survival time of female moths was longer than that of male moths. Both male and female moths fed with KME containing diet (200 or 400 ${\mu}g$/head/day) showed significantly higher mean survival times than those of the control moths. Studying the effect of KME on prolongation of mean survival time showed that male moths were more susceptible than female moths. The moths fed with KME-containing diet showed a slight decrease in body weight. Interestingly, however, no difference in food intake was observed between moths fed with KME-containing diet and those with control diet. In analysis for mRNA increase in the SIRT2 gene, a member of the Sir2 gene family playing important roles in regulation of cell death and prolongation of life span, moths fed with KME-containing diet showed a significant increase in SIRT2 gene expression. These results suggest that KME has a potential to extend the life span in Bombyx mori, and its effect is partly associated with increase in SIRT2 gene expression.

• Journal of Microbiology and Biotechnology
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• 제25권1호
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• pp.89-97
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• 2015

Bacillus subtilis HK176 with high fibrinolytic activity was isolated from cheonggukjang, a Korean fermented soyfood. A gene, aprE176, encoding the major fibrinolytic enzyme was cloned from B. subtilis HK176 and overexpressed in E. coli BL21(DE3) using plasmid pET26b(+). The specific activity of purified AprE176 was 216.8 ± 5.4 plasmin unit/mg protein and the optimum pH and temperature were pH 8.0 and 40℃, respectively. Error-prone PCR was performed for aprE176, and the PCR products were introduced into E. coli BL21(DE3) after ligation with pET26b(+). Mutants showing enhanced fibrinolytic activities were screened first using skim-milk plates and then fibrin plates. Among the mutants, M179 showed the highest activity on a fibrin plate and it had one amino acid substitution (A176T). The specific activity of M179 was 2.2-fold higher than that of the wild-type enzyme, but the catalytic efficiency (k_cat/K_m) of M179 was not different from the wild-type enzyme owing to reduced substrate affinity. Interestingly, M179 showed increased thermostability. M179 retained 36% of activity after 5 h at 45℃, whereas AprE176 retained only 11%. Molecular modeling analysis suggested that the 176^th residue of M179, threonine, was located near the cation-binding site compared with the wild type. This probably caused tight binding of M179 with Ca²⁺, whichincreased the thermostability of M179.

• 한국환경교육학회지:환경교육
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• 제19권3호
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• pp.40-56
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• 2006

The purpose of this study is to enhance elementary students' awareness of environment-friendly life and help them to prepare for a better life in the future. To achieve this purpose we examined the effect typical environmental experience-based learning activities, which were based on the local circumstances with high environmental-educational potential, have on the attitudes toward environment-friendly life. This study was carried out on the basis of typical environmental experience-based learning in the small class size. The research group used was composed of one sixth grade elementary school class called Sangroksu, whose total students were 9. The research period lasted from March 2005 to February 2006. To analyze the result of this study, two research methods were applied simultaneously : quantitative research methods and qualitative research methods. Especially statistical analysis in quantitative research methods by self-administrated questionnaire was done with SAS program. Qualitative research methods were analyzed in a cyclic pattern, including the processes of domain analysis, classification analysis, and factor analysis which continued to be associated with data-collecting methods. This research shows the following results. First of all, students have shown meaningful differences after typical environmental experience-based learning activities.(p<.05). Followings are fields of the differences - students‘ interest on the subject, their understanding levels of necessity for basic environmental facilities around us as well as for the kinds of environmental experience-based learning, awareness levels of various environmental problems, consciousness on environment conservation, and the practicing ability of environment - friendly lifestyles. Secondly, We have discovered improvements in the following fields after this study - the knowledge and understanding levels on our environment and human relationships, students' fundamental abilities to work out environmental problems, right ideas and appropriate attitudes on environment protection, the practicing ability of environment-friendly life styles, and their parents' understanding levels on the education related to environment. In conclusion, typical environmental experience-based learning activities have a positive effect on the improvement of elementary school students' environment-friendly life styles.

• Nutrition Research and Practice
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• 제1권2호
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• pp.100-104
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• 2007

Saururus chinensis Baill was reported to inhibit ${\alpha}-glucosidase$ in vitro and flatten postprandial increase in blood glucose in streptozotocin (STZ)-induced diabetic rats. We studied the effect of chronic consumption of S. chinensis Baill on blood glucose and lipid profile in STZ-induced diabetic male rats fed high fat diet. Male rats weighing 100-120 g were fed 30% fat diet with and without 10% freeze-dried leaves of S. chinensis Baill for 7 weeks after 1 week of adaptation. The rats were rendered diabetic by intravenous injection of STZ (60 mg/kg) after 6-week feeding of the assigned diets. At 1 week after the injection, the rats were sacrificed after an overnight fast. Plasma glucose ($380.2{\pm}14.4mg/dL$), total cholesterol ($93.9{\pm}7.9mg/dL$) and triglyceride levels ($123.6{\pm}7.5mg/dL$) of the S. chinensis Baill group were significantly lower than those of the control group ($418.1{\pm}12.0mg/dL,\;119.9{\pm}9.4mg/dL,\;152.0{\pm}10.3mg/dL$, respectively, p<0.05). Chronic consumption of S. chinesis Baill significantly decreased maltase activity of the small intestinal mucosa ($120.1{\pm}8.7U/g$) protein compared with the control group ($96.8{\pm}7.0U/g protein, p<0.05). These results suggest that S. chinensis Baill have hypoglycemic and hypolipidemic effects by inhibiting ${\alpha}-glucosidase$ activity in the animal model of diabetes mellitus.

• 기술사
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• 제39권1호
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• pp.50-53
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• 2006

I forecasted how much IT and ubiquitous technology will grow after 20 years. First of all, 1 think the goal of ubiquitous technology and relative main technology is our life. 1 will give examples of ubiquitous technology that affects travel, school and family life and how much it has changed. Lastly I will check the problems and securities in ubiquitous technology. By that time, we will have meaningful time to inspect the change of future technology.

• 호스피스학술지
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• 제8권1호
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• pp.13-28
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• 2008

목적: 본 연구는 학령기 아동의 죽음인식에 대한 탐색적 연구를 통하여 그들의 죽음인식에 대한 주관적 구조와 유형을 탐색하여 학령기 아동에게 죽음인식을 이해하며 효과적인 죽음준비교육 프로그램을 개발 시행하기 위한 기초자료를 제공하고자 시도하였다. 방법: 학령기 아동의 죽음인식에 관한 주관적인 구조와 유형을 탐색할 수 있는 Q방법론을 사용하였다. Q-모집단은 학령기 아동 20명을 대상으로 중립적 면담과 개방형 질문지를 이용하고 문헌연구를 통하여 총 132개의 진술문을 수집하였으며. Q표본은 비 구조화된 방법을 통해 23개의 진술문(Q-표본)을 도출하였다, P표본은 학령기 아동 31명(8세-13세)이었으며 Q카드를 이용하여 Q-분류를 실시하였으며, 수집된 자료는 PC QUANL 프로그램을 사용하여 분석하였다. 결과: 학령기 아동의 죽음인식 유형은 5개의 유형으로 분류되었다. 제 1 유형은 기능적형으로 죽음의 구성요소인 비역행성, 보편성, 비기능성, 인과성에 대한 주관적 인식이 두드러진 유형이었다. 제 2 유형은 사후 세계형으로 기독교의 종교적 배경을 가진 아동들과 직계가족의 죽음을 경험을 했으며 사후세계에 대하여 강한 내세 중심의 죽음인식이 두드러진 유형이었다. 제 3 유형은 종교형으로 죽어서도 가족과 친구들을 지켜볼 수 있다는 믿음이 강하기 때문에 사후세계에 대한 긍정적인 믿음이 확실한 유형이었다. 제 4 유형은 공포형으로 죽음에 대한 공포가 타 유형에 비하여 강한 유형이었다. 제 5 유형은 현실형으로 좋은 죽음에 대한 인식에 강한 긍정적인 동의를 보이는 유형이었다. 결론: 본 연구결과가 주는 간호학적 의의는 다음과 같다. 간호실무면에서 학령기 아동의 죽음인식의 주관성을 이해하는데 있어서 기존의 문헌이나 연구에서 중점적으로 제시하고 있는 죽음의 구성요소에 대한 이해의 차원에서 좀 더 확대되어 학령기 아동이 인지하는 죽음의 정의, 사후세계, 좋은 죽음에 대한 인지수준을 이해하고 유형별 특성에 따라 학령기 아동들의 효과적인 죽음준비교육 프로그램을 개발하는데 유용한 자료로 사용하여, 청소년기에 나타나는 죽음 경시풍조 및 만연된 자살을 예방하고 올바른 삶을 살아갈 수 있도록 밑거름이 되는 역할을 담당 할 것이다. 간호이론 면에서 학령기 아동의 죽음인식의 주관적 구조와 유형별 특성을 규명하고 탐색함으로써 학령기 아동의 죽음인식 모델 구축과 나아가 생명존중의 유용한 자료로 활용될 수 있다. 간호연구 면에서 학령기 아동에게 유형별로 긍정적인 죽음인식과 더 나아가 생명 존중의 측면에서 심리사회적 간호를 제공하기 위한 도구의 개발과 간호중재 전략의 효과를 규명하는 연구에 기여할 수 있다.

• 한국정밀공학회:학술대회논문집
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• 한국정밀공학회 2006년도 춘계학술대회 논문집
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• pp.129-130
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• 2006

This paper presents a study on the effect of feedrate changes on the fatigue life of Al 7075-T6 specimens before and after shotpeening. For this purpose. five groups of specimens, each of which consists of eight specimens, were first machined at five different feedrates (0.05, 0.10, 0.15, 0.20 0.25 mm/rev). Half of eight specimens in each group were then peened and the others remained un-peened Finally, the fatigue life of each specimen was measured by using 4-point rotatry bending machine. Comparison of fatigue life among ten sets of specimens is presented.

• 산경연구논집
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• 제13권12호
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• pp.23-31
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• 2022

Purpose: This research is to answer the following research questions because of the importance of learning how retired businesspeople increase their financial security and quality of life. (1) how does an entrepreneur contribute to economic development? (2) what types of investments are performed by businesspeople after retirement? (3) how entrepreneurial financial attainment capacity and anticipated net income after retirement are related? Research design, data and methodology: This research has conducted the qualitative analysis (QCA) using the current literature review. In the traditional positivist research paradigm, the quality of research is evaluated based on issues of validity, reliability, and objectivity. Contrary to the positivist approach, QCA focuses on the underlying meaning of the data. Results: This research found that finance management is increasingly challenging for businesspeople. The solutions based on the literature investigation suggest four categories. (1) Planned spending and revenue allocation, (2) Investing in the stock market, (3) Create a culture of saving, (4) Get Informed. Conclusions: This research puts forth a model to describe the connection between an investor's financial achievement potential and their desired net profits in retirement. The results of this study support the idea that the ability to achieve financial success as an entrepreneur has a salutary effect on the amount of money saved for retirement.

• BMB Reports
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• 제37권5호
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• pp.538-545
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• 2004

A new CRT binding factor (CBF) gene designated Cbcbf25 was cloned from Capsella bursa-pastoris, a wild grass, by the rapid amplification of cDNA ends (RACE). The full-length cDNA of Cbcbf25 was 898 bp with a 669 bp open reading frame (ORF) encoding a putative DRE/CRT (LTRE)-binding protein of 223 amino acids. The predicted CbCBF25 protein contained a potential nuclear localization signal (NLS) in its N-terminal region followed by an AP2 DNA-binding motif and a possible acidic activation domain in the C-terminal region. Bioinformatic analysis revealed that Cbcbf25 has a high level of similarity with other CBF genes like cbf1, cbf2, and cbf3 from Arabidopsis thaliana, and Bncbf5, Bncbf7, Bncbf16, and Bncbf17 from Brassica napus. A cold acclimation assay showed that Cbcbf25 was expressed immediately after cold triggering, but this expression was transient, suggesting that it concerns cold acclimation. Our study implies that Cbcbf25 is an analogue of other CBF genes and may participate in cold-response, by for example, controlling the expression of cold-regulated genes or increasing the freezing tolerance of plants.