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Effects of Slow Release Recombinant Porcine Somatotropin (rpST) Administration on Growth Performance and pST and IGF-1 of Blood in Finishing Pigs (지속형 유전자 재조합 pST(Recombinant Porcine Somatotropin; rpST) 투여가 비육돈의 성장, 혈중 pST 및 IGF-1 농도에 미치는 영향)

  • 김영화;문홍길;박준철;정현정;김인철;이상진;장병선;정정수;정일병
    • Journal of Animal Science and Technology
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    • v.48 no.5
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    • pp.663-670
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    • 2006
  • The present study was performed to investigate the effect of plant type recombinant porcine somatotropin (pST) adminstration on growth performance and blood profile of finishing pigs. Forty-eight Landrace barrows weighing 78 kg were employed for six week growth trial. Twelve barrows were allocated into various rpST types ; the control (CONT), the 4:1 type rpST (TRT 1), the 1:1 type rpST (TRT 2); and the 4:1 type rpST group(TRT 3) respectively. CONT group were not planted rpST. TRT 1 group was treated with rpST once a week for 6 weeks. Each dose contained the rpST equivalent to 100mg from initial to two weeks and 125mg from three weeks to finial week. TRT 2 and TRT 3 groups were planted rpST four times contained the rpST equivalent to 100mg during trial period from initial to two weeks, and 125 mg from three weeks, to four weeks, respectively. All pigs were fed a commercial feed containing 0.9% lysine ad lib. Daily gain increased by 19.4% in TRT 1(p<0.05) compared to the CONT. rpST improved feed/gain by 13.4~28.9% in all treatment groups(p<0.05). Back fat thickness of the all rpST treated groups were 23.3~29.2% thinner than that of the CONT. These results indicated that the rpST stimulated growth performance in finishing pigs and plant type rpST could be used as a growth stimulant for finishing pigs.

Effect of Immunoglobulin Y on Growth Performance and Blood Immunological Parameters in Holstein Calves (난황항체(IgY) 급여가 Holstein 송아지의 성장능력 및 면역관련 혈액 지표에 미치는 영향)

  • Jung, Hee-Sung;Jung, Keun-Ki;Jang, In-Surk
    • Journal of Animal Science and Technology
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    • v.51 no.4
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    • pp.321-328
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    • 2009
  • A total of fourteen, 1-wk-old male Holstein calves were allotted into two groups consisted of control (CON) and IGY which was orally administrated with immunoglobulin yolk (IgY) for 1wk. Calves in both groups were provided with milk replacer according to feeding program and had ad libitum access to timothy hay for the entire experimental period (7wks). At 0, 7 and 49 day of experiment, blood samples were collected from the jugular vein of calves to investigate blood biochemical profiles and the differential count (%) of white blood cell (WBC). We also monitored growth performance and colony forming unit (CFU) of fecal microbial population in calves. The adminstration of IgY in calves did not affect body weight and weight gain during 49 days feeding trial compared with control group. The CFU of E. coli and Lactobacilli in the feces of calves were not significantly affected by IgY treatment, whereas the score of the calf scours during day 43 to 49 in IgY group showed a significant (P<0.05) solid type. There were no differences in plasma biochemical components including total protein, albumin, immunoglobulin and the other indicators. As for WBC differential count (%), there was no statistical difference in the percentages of neutrophil, lymphocyte, monocyte, eosinophil and basophil at 0, 7 and 49 days after the oral supplementation of IgY. In conclusion, the oral supplementation of IgY as an immunostimulant did not affect growth performance, fecal microbial population, blood biochemical profile and WBC differential count in Holstein calves.

Studies on Proteolytic and Fibrinolytic Activity of Bacillus subtilis JM-3 Isolated from Anchovy Sauce (멸치액젓으로부터 분리한 Bacillus subtilis JM-3의 단백질 분해활성과 혈전 용해 활성에 관한 연구)

  • Lee, Sang-Soo;Kim, Sang-Moo;Park, Uk-Yeon;Kim, Hee-Yun;Shin, Il-Shik
    • Korean Journal of Food Science and Technology
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    • v.34 no.2
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    • pp.283-289
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    • 2002
  • This study was performed to search for potential microorganism that has rapid fermenting and physiological function from anchovy sauce. We isolated three bacterial strains, JM-1, JM-2, and JM-3 with proteolytic and fibrinolytic activity from anchovy sauce. Among the 3 bacterial strains, JM-3 showed the strongest proteolytic and fibrinolytic activity. Bacterial strain JM-3 was gram-positive rod, motile and formed endospore. The 16S rRNA of bacterial strain JM-3 was amplified by PCR and then its sequence was determined by ABI 310 genetic analyzer. The 16S rRNA sequence of bacterial strain JM-3 was compared to BLAST DNA database and identified to Bacillus subtilis with 99% of homology. The optimum temperature, pH and NaCl concentration for growth of B. subtilis JM-3 were $40^{\circ}C$, 5.0 and 0%, respectively. The optimum temperature, pH and NaCl concentration for proteolytic and fibrinolytic enzyme production of B. subtilis JM-3 were same as optimum conditions for growth. At 20% of NaCl concentration which is common NaCl concentration of fish sauce, B. subtilis JM-3 showed about 60% of proteolytic and fibrinolytic activity of 0% NaCl concentration. From above results, we found that B. subtilis JM-3 will be able to used for starter of functional fish sauce.

Adsorption Characteristics of Dimetridazole Antibiotics on Activated Carbon Prepared from Agricultural Waste Citrus Peel (폐감귤박 활성탄을 이용한 항생제 Dimetridazole의 흡착특성)

  • Lee, Chang-Han;Kam, Sang-Kyu;Lee, Min-Gyu
    • Korean Chemical Engineering Research
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    • v.55 no.6
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    • pp.798-806
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    • 2017
  • A activated carbon (WCAC, waste citrus activated carbon) prepared from an agricultural waste citrus peel material generated in Jeju was utilized for the removal of dimetridazole (DMZ) antibiotics in aqueous solution. The adsorption of DMZ on WCAC was investigated with the change of various parameters such as contact time, dosage of WCAC, particle size of WCAC, temperature, pH, and DMZ concentration. The DMZ adsorption capacity increased with increasing temperature and decreasing particle size. Also it was decreased at less than pH 4 but sustained almost constantly at pH 4 or greater. Isotherm parameters were determined from the Langmuir, Freundlich, Redlich-Peterson and Duinin-Radushkevich (D-R) isotherm models. The isotherm data were best described by the Redlich-Peterson isotherm model. And the adsorption kinetics can be successfully fitted to the pseudo-second-order kinetic model. The results of the intra-particle diffusion model suggested that film diffusion and intra-particle diffusion were occurred simultaneously during the adsorption process. Meanwhile, the thermodynamic parameters indicated that the adsorption reaction of DMZ on WCAC was an endothermic and spontaneous process. The experimental results showed that WCAC is a promising and cheap adsorbent for the removal of DMZ antibiotics.

Effects of Sex Steroid Hormones and High Temperature on Sex Differentiation in Black Rockfish, Sebastes schlegeli (성스테로이드 호르몬과 고수온 처리가 조피볼락, Sebastes schlegeli의 성분화에 미치는 영향)

  • LEE Chi Hoon;NA Oh Soo;YEO In Kn;BAEK Hea Ja;LEE Young Don
    • Korean Journal of Fisheries and Aquatic Sciences
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    • v.33 no.5
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    • pp.373-377
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    • 2000
  • This study Investigated the effects of $estradiol-l7{\beta} (E_2)$, $17{\alpha}-methyltestosterone$ (MT) and high temperature (WT) on gonadal sex differentiation in black rockfish, Sebastes srhlegeli. fish were reared to oral adminstration of E, at nominal concentrations of $20, 40 and 60 {\mu}g/g diet$, and MT at nominal concentration of 20 and $50 {\mu}g/g$ diet from 56 days to 77 days after parturition. In the treatment of WT, water temperature of the breeding tanks ranged $27.0{\pm}0.5^{\circ}C$ more than $10^{\circ}C$ approximately, in comparison to the control and other experimental group, In the process of sex differentiation until 56 days after parturition, gonads were composed of mostly gonia cells, sexually undifferentiated. In contrast, 128 dal's after parturition, the ovaries were composed of ovarian cavity and lamellae, and oogonia and perinucleolus oocytes were distributed in the lamellae of ovary, and the testes were composed of a number of seminiferous tubule, and spermatogonia were distributed in the seminiferous tubule, and also melanophore scattered in the matrix layer of testis. In the sex ratio, more females than male were observed from $E_2$. treatment groups when compared to the control, but more males than females were observed from MT and WT treatment groups when compared to the control. In the results of the present study, the concentration and kinds of the sex steroid hormones, and also the rearing high temperature caused to the factor of sex determination in the process of sex differentiation of black rockfish.

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Chemical compositions and antioxidant activities of Cheonnyuncho (Opuntia humifusa) stems and fruit (천년초의 성분특성과 항산화 활성)

  • Shin, Dong-Sun;Han, Gwi-Jung
    • Food Science and Preservation
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    • v.23 no.1
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    • pp.89-96
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    • 2016
  • The purpose of this study was to investigate the chemical composition of the freeze-dried stems and fruit of the cactus Cheonnyuncho. The analysis showed that powdered stems have the highest fat content (1.91%) and the powdered fruits have he highest protein content (2.62%). The K content of the fruits higher than that of the stems, while the Ca, Mg, Na and P contents of the stems were higher than those of the fruits. Both the stems and fruits powders contained high levels of the amino acids glutamic acid and aspartic acid. The free sugars such as sucrose, fructose, and glucose were detected in both the stems and fruits. The 75% ethanol (EtOH) extract showed a relatively high antioxidative activity compared to those of the water and 75% methanol (MeOH) extracts. Furthermore, the 75% EtOH extract of the stem powder exhibited a total polyphenol content of 3.60 g/100 g, and a total flavonoid content of 2.00 g/100 g. The antioxidant activities of the stem and fruit powder extracts, measured in DPPH radical scavenging experiments, were higher than that of the control group.

On the Change of Blood and Serological Pictures of the Sarcoma-180 Mouse and that Mouse treated with Korean Ginseng Extracts (肉腫癌쥐와 高麗人蔘을 處理한 肉腫癌쥐의 血液學的 變化에 관하여)

  • 이유윤
    • The Korean Journal of Zoology
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    • v.23 no.4
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    • pp.229-238
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    • 1980
  • The present experiment was carried out to observe the effect of Korean ginseng extract on the blood picture of mice that have been infected with Sarcoma-180. The observation of standard group was made for 2 days, 7 days, 12 days, 15 days after the injection of Sarcoma-180 and the observation of ginseng extract group was made at 15 days after the injection of Sarcoma-180 and simultaneously with oral adminstration of ginseng extract every day for 15 days. 1. For 15 days after the injection of Sarcoma-180 in the standard group, the numbers of R.B.C. and platlet were not changed significantly, but the number of W.B.C. increased at the first stage, and then decreased suddenly after the middle stage. However, the number of W.B.C. was not severely decreased in the ginseng extract group. This phenomenon is estimated that ginseng extract assisted for W.B.C. to protect living body. 2. Serum protein picture of standard group was appeared as a nonselective protein losing pattern, and ginseng extract group was likely that of standard group, but it was not severe than that of standard group. This phenomenon is believed that ginseng extract gave some delay or immunization for Sarcoma-180 mouse against the development of Sarcoma-180. 3. Serum cholesterol quantities of standard group increased gradually until middle stage, but decreased in the last stage. But serum cholesterol quantity of ginseng extract group showed a slight increase. What the decrease of cholesterol quantity in the last stage of standard group is caused especially by the decreased intaking of feed among many causes, it may be the cause of intaking not decrease of feed in ginseng extract group, as the ginseng extract gave the Sarcoma-180 mouse some resistance against the development of Sarcoma-180. 4. Along with developing of Asrcoma-180 in the standard group, serum total lipid quantities increased, and $\\alpha-, \\beta$-lipoprotein quantities showed decrease phenomenon, but pre $\\beta$-lipoprotein quantities increased rapidly. The value of ginseng extract group was appeared the value in the first stage of standard group. This phenomenon was also estimated that ginseng extract protected the living body according to above theories, and that ginseng extract gave the Sarcoma-180 mouse some resistance against the development of Sarcoma-180.

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Protective effects of dandelion extract against liver damage by repeated administration of thioacetamide in rats (랫드에서 thioacetamide의 반복 투여로 유도된 간 손상에 대한 민들레 추출물의 효과)

  • Moon, Sun-Jin;Shin, Sung-Shik;Son, Chang-Ho;Oh, Ki-Seok;Kim, Ha-Jung;Jung, Ji-Young;Suh, Guk-Hyun
    • Korean Journal of Veterinary Service
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    • v.40 no.2
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    • pp.107-117
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    • 2017
  • This study was conducted to investigate of hepatoprotective effect of dandelion water extract (DWE) according to repeated administration of thioacetamide (TAA) induced hepatotoxicity in Spraque-Dawley rats. Thirty rats were randomly assigned to 5 groups; normal control, DWE-control, TAA-control (TAA injection during the feeding of normal diet), TAA&DWE600 (TAA repeated injection during the feeding of DWE 600 mg/kg BW), TAA&DWE1200 (TAA repeated injection during the feeding of DWE 1,200 mg/kg BW). Rats in DWE-control and TAA&DWE groups were treated with DWE (600 or 1,200 mg/kg BW daily) by gavage for 20 days (twice a day). All the rats in the TAA-control and TAA&DWE groups were repeated injection of TAA (100 mg/kg BW) into the abdominal cavity 3 days interval and 12 hrs later, all rats were sacrificed. At the same time, normal control and DWE-control groups were injected normal saline. In TAA&DWE groups, serum alanine and aspartate aminotransferase (ALT, AST) were significantly decreased and triglyceride (TG) synthesis was significantly increased compared to TAA group. As well as total billilubin and GGT were slightly decreased by the treatment of DWE. Lipid peroxidation (MDA) concentration was significantly decreased and hepatic GSH content was slightly or significantly increased in the TAA&DWE groups compared to TAA group. Hepatic anti-oxidative enzyme activities, such as GSH, GST, SOD and catalase were slightly or significantly elevated by the treatment of DWE. According to these results, When dandelion extract was long term supplied, it could be used as a potential protective material for a longer time liver damage by repeated adminstration of the TAA.

Floral Characteristics and Cross Compatibility of Collected Paeonia lactiflora PALL (작약(芍藥) 수집종(蒐集種)의 화기특성(花器特性)과 교잡친화성(交雜親和性))

  • Hwang, Hyung-Baek;Kim, Jae-Chul;Choi, Jang-Soo;Choo, Yeun-Dai;Choi, Boo-Sull
    • Korean Journal of Medicinal Crop Science
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    • v.3 no.1
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    • pp.25-29
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    • 1995
  • In order to obtain the basic information on cross breeding of peony, the growth and floral characteristics were investigasted for local collected strains in main cultvation area in Korea. Local peony strains were classified by early emergence lines and late emergence lines according to the emergence date. Flower color of single-petaling flower was varied with red, pink and white, on the other hand, red+red, pink+white, pink+red and pink+pink in double-petaling flower. Number of pistil of the most local peony strains was 3-4/flower, number of stamen was varied from 0 to more than 200 ea/flower, classifing by 4 groups according to the diameter of flower. Most of the cross combinations showed the cross comptibility, and number of follicle was 3-5 ea/flower, average number of seed was 15.6 ea/follicle, seed size was grouped : by small grains with less than 10g, medium grains with 10-15g and large grains with more than 15g of 100 seed weight.

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A Study on a Direction of Modification of the Trade and International Business Curriculum for Global Trade Expert (글로벌무역인력 양성을 위한 합리적인 무역학 교과과정 개편방향에 관한 연구)

  • Park, Kwang-So;Yu, Kwang-Hyun
    • THE INTERNATIONAL COMMERCE & LAW REVIEW
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    • v.37
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    • pp.329-360
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    • 2008
  • In Korea, the major of international trade and business introduced in the 1960s to supply trade expert to trading companies, and the heyday of the trade major was in the 1980s. However the trade major fell into decay from the middle of 1990s. The purpose of trade education in university is to supply high qualified employees to societies and companies. The problem is that companies have difficuties for recruiting of trade expert, on the other hand university graduates have few job oppertunies. It has some discrepancies between education and field. The object of this paper is to propose a direction of modification of the trade and international business curriculum for global trade expert. We research international trade and business departments' curriculums of GTEP universities. GTEP stands for global trade expert incubating program started from 2006 which have charge of MOCIE and KITA. First, in the title of department, a lot of former researchers have taken up the position to return "Trade" or "International Trade". We think more important fact is not the title of department but the curriculum, so we insist on a harmony between the department title and curriculums. The focus of modification of curriculum is to educate global trade expert, so we need to know about global business environment and companies' needs also. Second, We propose the directions for modification of curriculum are "convergence" and "specialty". Trade major is a mixed study in nature and trade major try to treat lots of subjects such as trade, business adminstration, economics, international law, international commerce, logistics and marketing etc to catch up changing global business circumstanses and companies' needs. So convergence of adjacent field is very important in study and training. Specialty means selection and concentration strategy for global trade expert. It is difficult to learn every knowledge and skills for employer's needs in 4 years and 140 credits. A students who has studied basic subject in trade, management, economics can choose 1 or 2 specailty subject such as trade and e-commerce, global marketing, logistics and transportation, commerce and policy, servive trade, foreign language and cross culture etc. In concusion, the concept of convergence and specialty is not separation but harmony each other, so we propose to promote two concept together for modification of the trade and international business curriculum for training of global trade expert.

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