• Proceedings of the Plant Resources Society of Korea Conference
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• 2022.09a
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• pp.46-46
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• 2022

본 연구의 목적은 약용과 식용으로서의 활용도가 높은 일당귀[Angelica acutiloba (Siebold & Zucc.) Kitagawa]의 GA₃농도에 따른 생육특성을 확인하고, 최적의 재배기술 개발 및 지속가능한 안정적인 작물생산에 대한 기초자료의 제공에 있다. 연구재료는 강원도 태백시에 위치하는 일당귀 재배농가에서 2021년 10월에 채종한 종자를 구입하였고, 영주시 풍기읍에 위치한 연구온실에서 연구를 수행하였다. 일당귀의 종자를 증류수에 48시간 침지 후 GA₃농도 4조건(50 ppm, 100 ppm, 500 ppm, 1,000 ppm)에 24시간 처리 후 72구 포트에 파종 후 출아율과 생육특성을 조사하였다. 연구결과 일당귀 종자는 16일이 경과한 시점부터 출아를 시작하였고, 500 ppm처리구에서 73.3%로 가장 높은 출아율을 나타냈다. 생육특성조사는 출아 후 40일(1차)과 80일(2차) 경과된 시점에서 총 2회를 수행하였는데 1차 생육조사에서는 주근길이와 직경을 제외한 모든 생육특성에서 처리구별 생육의 차이가 확인되지 않았으나 2차 생육조사에서는 지상부와 지하부의 모든 생육특성에서 처리구별 생육의 차이가 확인되었다. 잎수(6.3개)와 주근길이(4.26 cm)는 500 ppm처리구에서 가장 높은 생육량을 나타냈고, 지상부 높이(21.81 cm), 잎길이(9.0 cm), 잎너비(10.77 cm), 주근직경(5.71 mm)은 1,000 ppm처리구에서 가장 높은 생육량을 나타냈다. 따라서 GA₃ 처리에 따른 일당귀의 생육특성은 생육초기보다 후기로 갈수록 발현량이 높게 나타나는 것으로 판단된다. 또한 저농도의 GA₃처리보다 500-1,000 ppm 사이의 처리농도가 적합한 것으로 판단되며, 추후 일당귀의 유용성분 분석을 통한 이화학적 연구가 추가된다면 최적의 재배기술 확립을 통한 안정적인 작물 생산에 기여할 수 있을 것이라 사료된다.

• Korean Journal of Plant Resources
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• v.7 no.1
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• pp.13-15
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• 1994

Some known coumarins, decursin, nodakenetin, umbelliferone, 7-demethylsuberosin, columbianetin, decursinol angelate and decursinol, showing significant cytotoxic activities against P388 cell lines,were isolated from the roots of Angelica gigas (Umbelliferae) . 7-Demethylsuberosin and columbianetinwere obtained from Angelica gigas for the first time. Chernotaxonornic difference about coumarins com-ponents between the roots of Angelica gigas and those of A. acutiloba is also discussed.

• Korean Journal of Medicinal Crop Science
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• v.24 no.4
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• pp.317-322
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• 2016

Background: In the herbal medicine market, Angelica gigas, Angelica sinensis, and Angelica acutiloba are all called "Danggui" and used confusingly. We aimed to assess the genetic diversity and relationships among 14 Angelica species collected from different global seed companies. Toward this aim we developed DNA markers to differentiate the Angelica species. Methods and Results: A total of 14 Angelica species, A. gigas, A. acutiloba, A. sinensis, A. pachycarpa, A. hendersonii, A. arguta, A. keiskei, A. atropurpurea, A. dahurica, A. genuflexa, A. tenuissima, A. archangelica, A. taiwaniana, and A. hispanica were collected. The genetic diversity of all 14 species was analyzed by using five chloroplast DNA-based simple sequence repeat (SSR) markers and employing the DNA fragment analysis method. Each primer amplified 3 - 12 bands, with an average of 6.6 bands. Based on the genetic diversity analysis, these species were classified into specific species groups. The cluster dendrogram showed that the similarity coefficients ranged from 0.77 to 1.00. Conclusions: These findings could be used for further research on cultivar development by using molecular breeding techniques and for conservation of the genetic diversity of Angelica species. The analysis of polymorphic SSRs could provide an important experimental tool for examining a range of issues in plant genetics.

• Journal of Physiology & Pathology in Korean Medicine
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• v.31 no.3
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• pp.182-187
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• 2017

The purpose of the present study is a comparison of the antioxidant effects of Angelica gigas Korea (AG), Angelica sinensis of China (AS), and Angelica acutiloba of Japan (AA), and comparison of the effects of AG, AS and AA on tight-junction related genes in human keratinocyte HaCaT cells. All species showed a strong antioxidant effect, and AA was higher than AG and AS in antioxidant effects. The cytotoxicity was confirmed to be higher in AS than AG and AA at a concentration of $1,600{\mu}g/ml$ using the MTS assay in HaCaT cells. We analyzed the effects of AG, AS, and AA on mRNA expression levels of various tight-junction related genes in HaCaT cells. We found that no obvious changes in expression of Claudin 1, 3, 4, 6, 7, 8, Occludin, JAM-A, ZO-1, ZO-2, and tricellulin by treatment of all species, suggesting that there is less possibility of side effects and skin moisturizing effects due to changes in tight-junction gene expression. Our results suggest that AG, AS, and AA are thought to be effective in reducing the oxidative stress of the skin and preventing the aging of the skin.

• YAKHAK HOEJI
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• v.49 no.6
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• pp.477-483
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• 2005

This study had been done for the investigation of the effect of Vitis vinifera extract (VV), Schisandra chinensis extract (SC), Taraxacum officinale extract (TO), Gardeniajasminoides extract (GJ), Angelica acutiloba extract (AA) and Paeonia japonica extract (PJ) on fatty liver and hepatotoxicity which was induced by Lieber-DeCarli ethanol liquid diet. Male Sprague-Dawley rats were randomly divided into eight groups: ethanol diet (ED), normal diet (ND), ED+VV (100mg/kg), ED+SC, ED+TO, ED+GJ, ED+AA, and ED+PJ (300mg/kg/day). Rats fed liquid diets for 6 weeks showed remarkable increase in serum and hepatic lipids indicating the onset of alcoholic fatty liver. The increasing levels of GPT, ALP activities in serum were observed in the groups fed with alcohol-containing diets compared to those of the ND group. The VV, SC, TO, GJ, AA and PJ groups were decreased the levels of triglyceride, free fatty acid and total cholesterol in serum and liver and GPT, ALP activities in serum. Therefore, they can be utiliaed as a health functional food or new drug candidate for fatty liver and hepatotoxicity which was induced by chronic alcohol consumption.

• Journal of Physiology & Pathology in Korean Medicine
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• v.20 no.5
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• pp.1155-1158
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• 2006

The purpose of this study is to examine the cytotoxicity of species of Angelica (Angelicas Radix; the root of Angelica gigas Nakai, A. sinensis (Oliv.) Diels, and A. acutiloba Kitag.) on HepG2 cells. The water extracts of roots of Angelica gigas (WAG), A. sinensis (WAS), and A. acutiloba (WAA) were studied for HepG2 cell viability by a modified MTT assay in the concentrations of 5, 10, 50, 100, 250, 500 ug/ml for 24, 48, 72 h. WAG and WAS did not reduced the cell viability significantly. But WAA reduced the cell viability in the concentration of 500 ug/ml for 24 h (85.45%), 48 h (75.01%). In conclusion, WAG and WAS have not the significant cytotoxicity on HepG2 cells in the suitable dose.

• YAKHAK HOEJI
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• v.50 no.4
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• pp.254-262
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• 2006

This study had been done for the investigation of the effect of Vitis vinifera extract(V), Schisandra chinensis extract (S), Taraxacum officinale extract (T), Gardenia jasminoides extract (G), Angelica acutiloba extract (A) and Paeonia japonica extract (P), and their mixtures on the antioxidant and ethanol oxidation system which was induced by Lieber-DeCarli ethanol liquid diet. Male Sprague-Dawley rats were randomly divided into eight groups: ethanol diet (ED), normal diet (ND), ED+V (100 mg/kg/day), ED+S, ED+T, ED+G, ED+A and ED+P (300 mg/kg/day). We studied the effect on alcohol dehydrogenase (ADH) and acetaldehyde dehydrogenase (ALDH) after herbal extracts administration for 6 weeks in rats induced by Lieber-DeCarli ethanol liquid diet. The differences in ADH and ALDH activity of the rats treated with herbal extracts and ED group were not significant. Phase I enzyme activity was found to be significantly higher in the ED+V than the ED group. Phase II enzymes (glutathione-S-transferase, phenol sulfatransferase) activities were found to be higher in the herbal extracts than the ED group. Herbal extracts not only reduced ethanol-induced elevation of level malondialdehyde but also protected against ethanol-induced decrease of reduced glutathione, gluthione reducatse, glutathione peroxidase, catalase and superoxide dismutase activities. Therefore, they can be utilized as a health functional food or new drug candidate for fatty liver and hepatotoxicity which was induced by chronic alcohol consumption.

• Journal of Ginseng Research
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• v.8 no.1
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• pp.1-7
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• 1984

In order to confirm the antimutagenicity of ginseng extracts, mutagenicity of ginseng and several medicinal herbs of which extracts were used as drinks, was examined through the method of Ames test. The obtained results were as follows. 1. Strong mutagenicities for Salmonella tyhimurium TA98, TA100, TA1535 were observed in all sample herbs Paenonia aalbiflora, Rehmannia glutinosa, Astragalus membranaceus, Angelica acutiloba, Cnidium officinale, Laurus nobilis and Panax ginseng without S-9 mix metabolic activation. 2. In the case of S-9 mix metabolic activation, even in a low concentraction, the extracts of Angelica autilobu, Cnidium officinale and Paenonla albtilora showed also a high mutagenicities for the strain TA98 and TA1535. 3. Even in high concentration of ginseng extracts, mutagenicity was almost neglectable through the metabolic activation of S-9 mix, compared with other extracts of medicinal herbs.

• YAKHAK HOEJI
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• v.6 no.1
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• pp.21-24
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• 1962

A report is given of microscopic study of the root Angelica gigas Nakai comparing with Ligusticum acutilobum S. et Z.(=Angelica acutiloba Kitagawa). The following characteristics are outstanding. 1. Angelica gigas Nakai is more tender and softer than Ligusticum acutilobum S. et Z. 2. Both Angelica gigas Nakai and Ligusticum acutilobum S. et Z. are alike in the form and arrangement of fundamental parenchyma in cortex and xylem. 3. No mechanical tissues are present but the substitute fibers in the Angelica gigas Nakai and Ligusticum acutilobum S. et Z. 4. The intercellular space are more numerous and larger in Angelica gigas Nakai than in Ligusticum acutilobum S. et Z. 5. Secretory tissue in Angelica gigas Nakai consists of numerous canals while in Ligusticum acutilobum S. et Z. very few.

• The Plant Pathology Journal
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• v.19 no.2
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• pp.79-84
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• 2003

Sclerotinia rot occurred severely on some vegetable crops grown in Namyangju, Yangpyung, and Yangiu areas in Korea in 2001-2002. The crops infected with Scterotinia sp. were Adenophora remotiflora, Armoracia lapathfolia, Angelica acutiloba, Angelica archangelica, Anthriscus sylvestris, Aster tataricus, Beta vulgaris var. cicla, Brassica campestris var. marinosa, Brassica juncea var. laciniata, Chicholium intybus, Lactuca indica var. dracoglossa, Lactuca sativa var. oak-leaf, Petroselinum crispum, and Phyteuma japonicum. The fungus associated with the disease was identified as Sclerotinia sclerotiorum, based on the morphological characteristics of the pathogen. The symptoms were water-soaked spots that enlarged later and became a watery soft rot. Infected parts became yellow and then turned brown, followed by death of the whole plant. White mycelia developed on the upper petioles and leaves and on the soil where these plant parts lay. Then black sclerotia in variable size and shape formed from the mycelial mass. Pathogenicity of the fungus was proven by artificially inoculating each crop. This is the first report of Sclerotinia rot on the listed vegetable crops in Korea.