• Journal of Life Science
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• v.17 no.4 s.84
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• pp.561-567
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• 2007

The present study was carried out to investigate the acute toxicity of Leuconostoc kimchii GJ2(Leu. kimchii GJ2), an exopolysaccharide-producing lactic acid bacteria isolated from kimchi, in ICR male and female mice. The test article was intraperitoneally or orally administered to both sexes of mice. Then followed a 14-day period of observation. In intraperitoneal acute toxicity test, mice were injected intraperitoneally with dosage of 0, 700, 900, 1,100, 1,300, 1,500, 1,700, 1,900, 2,100, 2,300 and 2,500 mg/kg. Intraperitoneal 50% lethal doses($LD_{50}$) of Leu. kimchii GJ2 was >2,500 mg/kg in male and female mice. In oral acute toxicity test, male and female mice were gavaged with Leu. kimchii GJ2 of five doses(0, 625, 1,500, 2,500 and 5,000 mg/kg). Oral $LD_{50}$ of Leu. kimchii GJ2 was consider to be higher than 5,000 mg/kg. Furthermore, no significant changes in general conditions, body weights, clinical signs and any gross lesions were observed in both sexes of mice administered intraperitoneally or orally with Leu. kimchii GJ2. In conclusion, our results suggest that no adverse effect level of Leu. kimchii GJ2 is estimated to be over 2,500 mg/kg in the intraperitoneal route and 5,000 mg/kg in the oral route, respectively.

• Food Science and Preservation
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• v.19 no.2
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• pp.315-321
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• 2012

The $in$ $vivo$ single-dose acute toxicity of $Lactobacillus$ $plantarum$ AF1, a lactic acid bacterium isolated from kimchi, in ICR male and female mice was investigated. The test article was intraperitoneally or orally administered once to both sexes of mice. The motalites, clinical findings, autopsy findings, and body weight changes were monitored daily for 14 days. In the oral acute toxicity test, the male and female mice were gavaged with four doses (5.0, 2.5, 1.25 and 0.625 g/kg) of $Lb.$ $plantarum$ AF1. The oral $LD_{50}$ of the $Lb.$ $plantarum$ AF1 was considered higher than 5.0 g/kg. In the intraperitoneal acute toxicity test, mice were injected intraperitoneally with dosages of 0.7, 0.9, 1.1, 1.3, 1.5, 1.7, 1.9, 2.1, 2.3 and 2.5 g/kg. The intraperitoneal 50% lethal dose ($LD_{50}$) of the $Lb.$ $plantarum$ AF1 was >2.5 g/kg in the male and female mice. No significant changes in the general conditions, body weights, clinical signs, and gross lesions were observed in both sexes of mice to which $Lb.$ $plantarum$ AF1 was administered intraperitoneally or orally. The results suggest that the no-adverse-effect level of $Lb.$ $plantarum$ AF1 is estimated to be more than 5.0 g/kg in the oral route and 2.5 g/kg in the intraperitoneal route.

• Herbal Formula Science
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• v.15 no.2
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• pp.113-117
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• 2007

Objectives: Samul-tang(Siwu-tang) has been traditionally prescribed a medicine as a restorative. Methods: In this study, we investigated the acute toxicity about water-extracted Samul-tang(Siwu-tang). Twenty-five mice completed 14 days of oral Samul-tang(Siwu-tang) at the respective doses of 0(control group), 2560, 3200, 4000 and 5000mg/kg. Results: We observed survival rates, general toxicity, change of body weight, and autopsy. Conclusions: Compared with the control group, we could not find any toxic alteration in all treated groups (2560, 3200, 4000 and 5000mg/kg). $LD_{50}$ of Samul-tang(Siwu-tang) was over 5000mg/kg and it is very safe to ICR mice.

• Journal of Korean Medicine for Obesity Research
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• v.9 no.1
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• pp.71-78
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• 2009

Objectives Bangpungtongsung-san(Fangfengtongsheng-san) has been traditionally prescribed a a restorative medicine. Methods In this study, we investigated the acute toxicity of water-extracted Bangpungtongsung-san(Fangfengtongsheng-san). 20 male and 20 female mice were orally treated of Bangpungtongsung-san(Fangfengtongsheng-san) at the respective doses of 0 (control group), 1250, 2500 and 5000mg/kg for 14days. Results We observed survival rates, general toxicity, change of body weight and examined microscopic changes of some organs. Conclusions Compared with the control group, we could no find any toxic alteration in all treated groups (1250, 2500 and 500mg/kg). $LD_{50}$ of Bangpungtongsung-san(Fangfengtongsheng-san) might be over 5000mg/kg and it was very safe to ICR mice.

• Journal of Acupuncture Research
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• v.15 no.2
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• pp.357-367
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• 1998

This experiment was carried out to study on the acute toxicity of Thesium Chinese Turczaninow solution for aqua-acupuncture. Test material was manufactured by 'Water Alcohol method'. Mice and rats were used for this test. According to this experimental results, there were not observed any acute toxicity with Thesium Chinese Turczaninow solution for aqua-acupuncture. So Thesium Chinese Turczaninow solition for aqua-acupuncture is considered a have level of cliniccal safety. It is expected to be clinically helpful, and it is considered that continuous study is necessary.

• Korean Journal of Pharmacognosy
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• v.45 no.2
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• pp.181-185
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• 2014

This study was carried out to investigate the acute toxicity of the bark of Diospyros kaki (Ebenaceae) in mice. The aqueous extract of the bark of Diospyros kaki (AEDK) was administered orally at a doses of 5 mg/kg, 50 mg/kg, 300 mg/kg and 2,000 mg/kg. In this study, number of deaths, clinical signs, body weights and pathological examinations were investigated after administration of AEDK. There were neither dead animals nor significant changes of body weights during the experimental period. In addition, no differences were found between control and AEDK treated groups in clinical signs, organ weights and gross pathological findings. AEDK did not show any toxic effect in mice.

• Journal of Food Hygiene and Safety
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• v.6 no.3
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• pp.185-189
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• 1991

This study was carried out to investigate whether acute toxicity of BST-SR is or not. Single dose of BST-SR was given to both sexes of Spraqe-Dawley rats and ICR mice, subcutaneously. No significant toxic symptom was observed in single treated rats and mice during the experimental period. In gross and microscopic observation, no significantly different abnormality observed between the several organs of tested animals and control animals. Therefore, it was concluded that BST-SR was nontoxic when BST-SR was subcutaneously administered to rats and mice up to 1000 times of clinical dose.

• Toxicological Research
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• v.18 no.1
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• pp.73-77
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• 2002

The single dose toxicity of Hwangiaegongjinbo, an invigorator developed by Korea Medical Science Institute was evaluated in ICR mice and Sprague-Dawley rats. The aqueous solution of freeze-dried powder of Hwangiaegongjinbo or its original solution was once administrated orally to both sexes of mice and rats at dose of 2000 mg/kg, the recommended upper limit dose for acute toxicity. Water was administered to another group as control. after single adminstration, sign of toxicity were observed every hour for the first 6 hours and every day for 14 days. Neither sign그cant toxic sign nor death was observed during the observation period. In addition, no pathological changes were noticed in macroscopic examination at necropsy in those treated group. These results indicated that $LD_{50}$ of Hwangiaegongjinbo is greater than 2000 mg/kg in ICR mice and Sprague-Dawley rats.

• The Korea Journal of Herbology
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• v.28 no.2
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• pp.61-65
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• 2013

Objectives : Samchulgeonbi-tang (shenzhujianpi-tang) has been prescribed as one of traditional herbal medicine for treatment of stomach diseases since ancient time in Korea. Samchulgeonbi-tang extract was fermented by Lactobacillus spp. for improving the effect. However, the toxicity and safety of fermented Samchulgeonbi-tang (FS) extract were not confirmed. Therefore, this study was performed to evaluate the acute toxicity and safety of FS extract. Methods : To evaluate the acute toxicity and safety of FS extract, several doses of FS extract, 0, 500, 1000 and 2000 mg/kg, were orally administered to 20 male and 20 female ICR mice, respectively. After treatment with FS extract, we observed mortality, general toxicity, behavior and change of body weight for the 14 days. After 14 days of oral administration, all mice were sacrificed and hematological parameters were analyzed from blood serum. Results : In present study, the toxic signs such as mortality or abnormal behaviors by FS extract were not observed. There are no significant differences between FS-treated group and control group in body weight, organ weights, and hematological parameters. Conclusions : The remarkable adverse effects by FS extract were not observed in ICR mice. Also, any death was not occurred at all treated FS doses, 500, 1000 and 2000 mg/kg. Therefore, the approximate lethal dose (ALD) of FS extract may be more than 2000 mg/kg.

• The Korean Journal of Food And Nutrition
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• v.11 no.6
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• pp.629-634
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• 1998

1-Deoxynojirimycin which is a potent intestinal ${\alpha}$-glucosidase inhibitor was purified from the culture broth by ion exchange chromatography, Sephadex LH20 column chromatography, TSK gel chromatography and HPLC respectively. Acute toxicity of 1-deoxynojirimycin, which was loaded through the oral as dose of 200mg/kg, was investigated in IRC mouse. None of the tested IRC mice were not dead and increase of body weight showed also the same results in comparison with control mice. The antimicrobial susceptibility of 20 pathogenic strains against 3 antidiabetic compounds (1-deoxynojirimycin, AO-128, acarbose) were obtained by agar dilution method. All of the three antidiabetic compounds has very weak antimicrobial activity (MIC>100$\mu\textrm{g}$/ml).