Browse > Article
http://dx.doi.org/10.5352/JLS.2007.17.4.561

Acute Toxicity of Leuconostoc kimchii GJ2, An Exopolysaccharide-Producing Lactic Acid Bacteria Isolated from Kimchi, in Mice  

Lee, Jae-Joon (Dept. of Food and Nutrition, Chosun University)
Lee, Yu-Mi (Dept. of Food and Nutrition, Chosun University)
Chang, Hae-Choon (Dept. of Food and Nutrition, Chosun University)
Lee, Myung-Yul (Dept. of Food and Nutrition, Chosun University)
Publication Information
Journal of Life Science / v.17, no.4, 2007 , pp. 561-567 More about this Journal
Abstract
The present study was carried out to investigate the acute toxicity of Leuconostoc kimchii GJ2(Leu. kimchii GJ2), an exopolysaccharide-producing lactic acid bacteria isolated from kimchi, in ICR male and female mice. The test article was intraperitoneally or orally administered to both sexes of mice. Then followed a 14-day period of observation. In intraperitoneal acute toxicity test, mice were injected intraperitoneally with dosage of 0, 700, 900, 1,100, 1,300, 1,500, 1,700, 1,900, 2,100, 2,300 and 2,500 mg/kg. Intraperitoneal 50% lethal doses($LD_{50}$) of Leu. kimchii GJ2 was >2,500 mg/kg in male and female mice. In oral acute toxicity test, male and female mice were gavaged with Leu. kimchii GJ2 of five doses(0, 625, 1,500, 2,500 and 5,000 mg/kg). Oral $LD_{50}$ of Leu. kimchii GJ2 was consider to be higher than 5,000 mg/kg. Furthermore, no significant changes in general conditions, body weights, clinical signs and any gross lesions were observed in both sexes of mice administered intraperitoneally or orally with Leu. kimchii GJ2. In conclusion, our results suggest that no adverse effect level of Leu. kimchii GJ2 is estimated to be over 2,500 mg/kg in the intraperitoneal route and 5,000 mg/kg in the oral route, respectively.
Keywords
Leuconostoc kimchii GJ2; exopolysaccharide-producing lactic acid bacteria; acute toxicity mice;
Citations & Related Records
Times Cited By KSCI : 2  (Citation Analysis)
연도 인용수 순위
1 Welmen, A. D. and I. S. Maddox. 2003. Exopolysaccharide from lactic acid bacteria: perspectives and challenges. Trends in Biotechnology. 21, 269-274   DOI   ScienceOn
2 Kim, B. J., B. H. Min, J. H. Kim and H. U. Han. 2001. Isolation of dextran-producing Leuconostoc lactis from kimchi. J. Microbiol. 39, 11-16
3 Vaninggelgem, F., M Zamfir, F. Mozzi, T. Adriany, M. Vancanneyt, J. Sings and L. D. Vuyst. 2004. Biodiversity of exopolysaccharide produced by Streptococcus thermophillus strains is reflected in their production and their molecular and functional characteristics. Appl. Environ. Microbiol. 70, 900-912   DOI   ScienceOn
4 Duboc, P. and B. Mollet. 2001. Application of exopolysaccharides in the dairy industry. Int. Dairy J. 11, 759-768   DOI   ScienceOn
5 Eom, H. J., D. M. Seo, H. S. Yoon, H. B. Lee and N. S. Han. 2002. Strain selection of psychrotrophic Leuconostoc mesenteriods producing a highly active dextransucrase from kimchi. Korean J. Food Sci. Technol. 34, 1085-1090   과학기술학회마을
6 Kang, H. J., S. C. Baick and J. H. Yu. 2005. Studies on the properties of the stirred yogurt manufactured by exopolysaccharide producing lactic acid bacteria. Korean J. Food Sci. Ani. Resour. 25, 84-91   과학기술학회마을
7 Kim, D. J. and S. Y. Lee. 2001. Isolation of exopolysaccharide producing Enterobacter sp. and physiochemical properties of the polysaccharide produced by this strain. Korean J. Biotechnol. Bioeng. 16, 270-375   과학기술학회마을
8 Kim, H. J. 2006. Isolation and characterization of the exopolysaccharide-producing lactic acid bacteria from kimchi. MS Thesis. Chosun University
9 Litchfield, J. T. and F. Wilcoxon. 1949. A simplified method for evaluation dose-effect experiments. J. Pharmacol. Exp. Ther. 96, 99-113
10 Kimmel, S. A., R. F. Roberts and G. R. Zieger. 1998. Optimization of exopolysaccharide production by Lactobacillus delbrueckii ssp. bulgaricus RR grown in a semidefined medium. Appl. Environ. Microbiol. 64, 659-664
11 Ruas-Madiedo, P., J. Hugenholtz and P. Zoon. 2002. An overview of the functionality of exopolysaccharides produced by lactic acid bacteria. Int. Dairy J. 12, 163-171   DOI   ScienceOn
12 Sutherland, I. W. 1998. Novel and established applications of microbial polysaccharide and optimization of its production. Korean J. Biotechnol. Bioeng. 17, 169-175
13 Cerning, J., C. Bouilanne, M. Desmazeaud and M. Landon. 1998. Exocellular polysaccharide production by Streptococcus thermophilus. Biotechnol. Lett. 10, 255-260   DOI
14 Bae, I. H. and J. W. Huh. 2002. Isolation of Lactobacillus ssp. producing exopolysaccharide and optimization of its production. Korean J. Biotechnol. Bioeng. 17, 169-175