• The Journal of Korean Medicine
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• v.33 no.2
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• pp.73-77
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• 2012

Objectives: This report reports a clinical case of a patient with recurrent aphthous stomatitis (RAS) for 10 years who was completely cured using Korean medicine. Methods: A male patient with terribly severe RAS was hospitalized, and then treated with Jibaekpalmi-hwon, indirect moxibustion (KI 1 and CV 4) and acupuncture. The clinical outcome was observed by self-reported VAS measurement and macroscopy. Results: The severity of stomatitis became improved gradually and completely recovered during 17 hospital days, and RAS has not recurred for three months as an outpatient. The blood test including complement activity was in the normal range. Conclusions: This case report provides information about a therapeutic effect of Jibaekpalmi-hwon and Korean medicine on RAS.

• The Journal of Korean Medicine Ophthalmology and Otolaryngology and Dermatology
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• v.36 no.2
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• pp.83-93
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• 2023

Objectives : The purpose of this case study is to report the effectiveness of Bangpoongtongsung-san gamibang on a patient with wind-heat type urticaria sensitive to aspirin and NSAIDs. Methods : We treated a 48-year-old man who had aspirin and NSAIDs sensitive wind-heat type urticaria with Bangpoongtongsung-san gamibang, pharmacopuncture(Hwangnyunhaedok-tang), acupuncture(needle remaining time : 20 min) and photodynamic therapy. Results : The Urticaria Activity Score(UAS) started at 5 and ended with 0, and the Visual Analog Scale(VAS) of heating sensation on whole body started at 10 and ended with 1-3. Conclusions : This study has demonstrated that Bangpoongtongsung-san gamibang could be effective to treat aspirin and NSAIDs sensitive wind-heat type urticaria.

• Journal of Korean Academy of Nursing
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• v.40 no.2
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• pp.191-201
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• 2010

Purpose: The aim of this study was to compare the predictors of physical activity between urban and rural dwelling Korean older adults. Methods: This study was a cross-sectional descriptive survey. A self-report questionnaire or face to face interviews were used to collect data from 336 older adults (urban: 129, rural: 207) who visited public health centers or welfare centers in 2008. Results: About half of the participants (urban: 50.4%, rural: 47.3%) were classified as the minimally active group. Cognitive function (odds ratio [OR]=1.106, p=.004) and loneliness (OR=0.965, p=.044) were predicting factors for physical activity in rural elderly. Age (OR=0.326, p=.037), gender (OR=2.841, p=.021) and depression (OR= 0.799, p<.001) were significant factors predicting physical activity in urban elders. Conclusion: These findings provide information that is relevant in designing interventions to enhance physical activity in older adults. There is a need to develop effective mutifaceted physical activity interventions that include reducing psychological barriers such as depression, loneliness.

• Journal of Microbiology and Biotechnology
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• v.12 no.5
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• pp.854-857
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• 2002

Bioassay-directed chromatographic fractionation of a methylene chloride extract of Ailanthus altisshima indicated the presence of 20(S), 24(R), epoxy-25-hydroxydammarane-3-one (compound 1, ocotillone) which was isolated from this plant, for the first time. Antimicrobial activity of compound 1 was measured by inhibition of bacterial and fungal cells growth and by a hemolytic assay with human erythrocytes, respectively. The results revealed that compound 1 had potent antibacterial activity against Cram-negative bacteria, P. aeruginosa and S. typhimurium, that were without hemolytic activity, whereas it had weak antimicrobial activity against Gram-positive bacteria and fungi. These results demonstrated that the compound 1 has more antibacterial activity against 6ram-negative bacteria, which have no hemolytic activity, than Gram-positive bacteria and fungi. This is the first report on the biological activities of the compound 1.

• Journal of Microbiology and Biotechnology
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• v.18 no.4
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• pp.616-623
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• 2008

In spite of an increasing interest in fucoidans as biologically active compounds, no convenient commercial sources with fucoidanase activity are yet available. A marine bacterial strain that showed confluent growth on a minimal medium containing fucoidan, prepared from Korean Undaria pinnatifida sporophylls, as the sole carbon source was isolated and identified based on a 16S rDNA sequence analysis as a strain of Sphingomonas paucimobilis, and named Sphingomonas paucimobilis PF-1. The strain depolymerized fucoidan into more than 7 distinct low-molecular-mass fucose-containing oligosaccharides, ranging from 305 to 3,749 Da. The enzyme activity was shown to be associated with the whole cell, suggesting the possibility of a surface display of the enzyme. However, a whole-cell enzyme preparation neither released the monomer L-fucose from the fucoidan nor hydrolyzed the chromogenic substrate p-nitrophenyl-${\alpha}$-L-fucoside, indicating that the enzyme may be an endo-acting fucoidanase rather than an ${\alpha}$-L-fucosidase. Therefore, this would appear to be the first report on fucoidanolytic activity by a Sphingomonas species and also the first report on the enzymatic degradation of the Korean Undaria pinnatifida sporophyll fucoidan. Moreover, this enzyme activity may be very useful for structural analyses of fucose-containing polysaccharides and the production of bioactive fucooligosaccharides.

• The Korean Journal of Nuclear Medicine Technology
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• v.13 no.3
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• pp.193-195
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• 2009

Purpose: The work flow of international hospital's laboratory consists of rapid test and result report at the present day. However, the frequent inquiry of sample, which cause affairs to delay and efficiency to Lower, affects medical examination. In order to promote work's efficiency, we should improve the problem and make work smooth between a laboratory, outs and ward. Materials and Methods: This study runs as follows. First, Investigating test result, test schedule, test receipt, quick result, etc through the activity required from September to November 2007 about the inquiry of sample. After analysis of the problem in December, remaking the test schedule for improvement solution and reporting it to outs and ward. When the result is retest and dilution, we directly fill in a result space with the result situation of the patient to let them know beforehand. We also, prevent the omission of the result through checking the sample list and discriminate in vivo from in vitro by changing the laboratory's telephone number. We have improved the problem about the inquiry of sample through valuation and analysis since the improvement activity from January to March 2008. Result: The case about the frequent inquiry of sample has reduced by 57.8%. this improvement activity indicated that p-value<0.05 was statistically significant through paired t-test. This activity make study smooth and we rapidly report the result. Conclusion: By reducing the case regarding the inquiry of sample, work discontinuation, and concentration reduction, the work efficiency was increased.

• Parasites, Hosts and Diseases
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• v.54 no.4
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• pp.477-484
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• 2016

There is renewed interest in natural products as a starting point for discovery of drugs for schistosomiasis. Recent studies have shown that phytol reveals interesting in vivo and in vitro antischistosomal properties against Schistosoma mansoni adult worms. Here, we report the in vitro antischistosomal activity of phytol against Schistosoma haematobium juvenile and adult worms and alterations on the tegumental surface of the worms by means of scanning electron microscopy. The assay, which was carried out with 6 concentrations (25, 50, 75, 100, 125, and $150{\mu}g/ml$) of phytol, has shown a promising activity in a dose and time-dependent manner. There was a significant decline in the motility of the worms and a mortality rate of 100% was found at 48 hr after they had been exposed to phytol in the concentration of $150{\mu}g/ml$. Male worms were more susceptible. On the ultrastructural level, phytol also induced tegumental peeling, disintegration of tubercles and spines in addition to morphological disfiguring of the oral and ventral suckers. This report provides the first evidence that phytol is able to kill S. haematobium of different ages, and emphasizes that it is a promising natural product that could be used for development of a new schistosomicidal agent.

• BMB Reports
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• v.45 no.3
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• pp.165-170
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• 2012

We report here that an ethanol extract of Tetracera scandens, a Vietnamese medicinal plant, has anti-HIV activity and possesses strong inhibitory activity against HIV-1 reverse transcriptase (RTase). Using a MT-4 cell-based assay, we found that the T. scandens extract inhibited effectively HIV virus replication with an $IC_{50}$ value in the range of 2.0-2.5 ${\mu}g$/ml while the cellular toxicity value (CC50) was more than 40-50 ${\mu}g$/ml concentration, thus yielding a minimum specificity index of 20-fold. Moreover, the anti-HIV efficacy of the T. scandens extract was determined to be due, in part, to its potent inhibitory activity against HIV-1 RTase activity in vitro. The inhibitory activity against the RTase was further confirmed by probing viral cDNA production, an intermediate of viral reverse transcription, in virus-infected cells using quantitative DNA-PCR analysis. Thus, these results suggest that T. scandens can be a useful source for the isolation and development of new anti-HIV-1 inhibitor(s).

• IMMUNE NETWORK
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• v.9 no.6
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• pp.243-247
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• 2009

In this study, we report the development of a new dual reporter vector system for the analysis of promoter activity. This system employs green fluorescence emitting protein, EGFP, as a reporter, and uses red fluorescence emitting protein, DsRed, as a transfection control in a single vector. The expression of those two proteins can be readily detected via flow cytometry in a single analysis, with no need for any further manipulation after transfection. As this system allows for the simultaneous detection of both the control and reporter proteins in the same cells, only transfected cells which express the control protein, DsRed, can be subjected to promoter activity analysis, via the gating out of all un-transfected cells. This results in a dramatic increase in the promoter activity detection sensitivity. This novel reporter vector system should prove to be a simple and efficient method for the analysis of promoter activity.

• Journal of Microbiology and Biotechnology
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• v.20 no.4
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• pp.732-736
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• 2010

Five strains of tannic acid degrading bacteria were isolated and identified by phenotypic characterization. All the five isolates showed cell-associated activity, whereas only three showed extracellular activity. Serratia ficaria DTC, showing the highest cell-associated activity (0.29 U/l), was selected for further shake-flask studies. Tannase synthesis was growth associated and reached the peak in the late stationary phase of growth. Organic nitrogen sources enhanced the tannase production. Peak tannase production of 0.56 U/l was recorded in the medium having the initial pH of 6. The pH and temperature optima of the enzyme were found to be 8.9 and $35^{\circ}C$, respectively. This is the first report of cell-associated activity in the case of bacterial tannase. Cell-associated tannase of Serratia ficaria DTC could be industrially important from the perspective of its activity at broad temperature and pH ranges, and its unusually high activity at pH 8.9.