• 감성과학
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• 제14권1호
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• pp.147-156
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• 2011

여러 연구자들이 정서특정적 자율신경계 활동을 밝히기 위하여 행복, 슬픔, 분노, 공포 및 혐오를 포함한 기본 정서들을 사용하여왔지만, Ekman 등(1983)의 기본정서들 중 놀람 정서에 대한 연구는 많지 않다. 본 연구의 목적은 ECG와 PPG를 이용하여 놀람 자극에 대한 심혈관 반응을 밝히는 것이다. 76명의 대학생에게 놀람자극을 제시하기 전과 후에 ECG와 PPG를 기록하였으며, ECG와 PPG 신호로부터 심박률(HR), R-R 간격의 표준편차(SD-RR), 연속된 R-R 간격 차이의 제곱평균제곱근(RMSSD-RR), 호흡성 부정맥(RSA), 손가락 혈류량 파형의 진폭(FBVPA), 손가락 맥파 전달시간(FPTT)을 산출하였다. HR과 SD-RR, RMSSD-RR은 놀람자극 제시 전에 비하여 놀람자극이 제시된 후에 유의하게 증가하였으며, FBVPA는 유의하게 감소하였고, FPTT는 유의하게 짧아졌다. 놀람 자극은 말초혈관을 수축시키고, 심박률을 증가시키는 교감신경계 반응특성을 가지는 것으로 볼 수 있으며, 심박률 변산성을 증가시키는 부교감신경계도 동시에 활성화될 가능성이 있는 것으로 나타났다. 이러한 연구 결과는 정서이론의 발전과, 인간정서를 탐지하는 컴퓨터 시스템의 기초를 확립하는데 기여하는 바가 있을 것이다.

• 한국축산식품학회지
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• 제40권6호
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• pp.1014-1032
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• 2020

This work aimed to investigate the psychobiotic effects of six bacterial strains on the mind and behavior of male Wistar rats. The probiotic (PRO) group (n=7) were rats pre-treated with antibiotics for 7 days followed by 14-day probiotic administration, antibiotics (ANT) group (n=7) were rats treated with antibiotics for 21 days without probiotics. The control (CON) group (n=7) were rats that received sham treatment for 21 days. The six bacterial strains with probiotic properties were mostly isolated from Thai fermented foods; Pedicoccus pentosaceus WS11, Lactobacillus plantarum SK321, L. fermentum SK324, L. brevis TRBC 3003, Bifidobacterium adolescentis TBRC 7154 and Lactococcus lactis subsp. lactis TBRC 375. The probiotics were freeze-dried into powder (6×10⁹ CFU/5 g) and administered to the PRO group via oral gavage. Behavioral tests were performed. The PRO group displayed significantly reduced anxiety level and increased locomotor function using a marble burying test and open field test, respectively and significantly improved short-term memory performance using a novel object recognition test. Antibiotics significantly reduced microbial counts in rat feces in the ANT group by 100 fold compared to the PRO group. Probiotics significantly enhanced antioxidant enzymatic and non-enzymatic defenses in rat brains as assessed using catalase activity and ferric reducing antioxidant power assay, respectively. Probiotics also showed neuroprotective effects with less pyknotic cells and lower frequency of vacuolization in cerebral cortex. This multi-strain probiotic formulation from Thai fermented foods may offer a potential to develop psychobiotic-rich functional foods to modulate human mind and behaviors.

• 한국콘텐츠학회논문지
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• 제7권11호
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• pp.175-181
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• 2007

디자인원형은 조형원리인 성상적인 면과 물리적인 성격의 형상적인 면으로 설명되어진다. 원형은 문화전반의 사상과 배경의 핵심으로 생활 공예 및 건축, 미술의 제작의도와 작업 행위에 해당되는 것으로 한 민족의 문화와 예술 그리고 생활 전반에 내재한 공통분모라 할 수 있다. 우리는 새로운 문화를 흡수, 통합하는 과정에서 시 공간을 축으로 하여 디자인 원형을 형성시켜왔다. 현대 한국 디자인의 원형은 조선 후기로 볼 수 있는데 상층문화와 기층문화가 융합하면서 활발한 문화가 전개되었고, 이런 조선의 문화와 예술은 오늘날 우리 디자인과 한국 미의 근간이 되어 있음은 부정할 수 없다. 디자인 원형을 형성시킴에는 지형과 기후 등과 같은 자연 환경적 요소가 무엇보다 중요하며 이런 자연 환경적 요소는 건축과 실내 환경의 형태뿐만 아니라 예술과 생활 공예 전반의 디자인 의식을 주도해왔다. 한국디자인은 우리 민족의 기질과 정서로서 명분과 본능에 충실한 장인 정신을 낳았고 이성보다는 감성적 유희성의 작업을 가능하게 하여, 재료와 형태감에 순응하게 하는 자연주의를 형성시켜왔다. 이와 같은 우리의 디자인 원형의 경로 추적과 연구는 미래 사회의 새로운 디자인 identity의 에너지로써 우리의 디자인 발전에 중요한 테마라 할 수 있다.

• Journal of Periodontal and Implant Science
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• 제38권sup2호
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• pp.317-324
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• 2008

Purpose: Aggregatibacter actinomycetemcomitans was associated with localized aggressive periodontitis, endocarditis, meningitis, and osteomyelitis. The cytolethal distending toxin (CDT) of A. actinomycetemcomitans was considered as a key factor of these diseases is composed of five open reading frames (ORFs). Among of them, An enzymatic subunit of the CDT, CdtB has been known to be internalized into the host cell in order to induce its genotoxic effect. However, CdtB can not be localized in host cytoplasm without the help of a heterodimeric complex consisting of CdtA and CdtC. So, some studies suggested that CdtC functions as a ligand to interact with GM3 ganglioside of host cell surface. The precise role of the CdtC protein in the mechanism of action of the holotoxin is unknown at the present time. The aim of this study was to generate recombinant CdtC proteins expression from A. actinomycetemcomitans, through gene cloning and protein used to investigate the function of Cdt C protein in the bacterial pathogenesis. Materials and Methods: The genomic DNA of A. actinomycetemcomitans Y4 (ATCC29522) was isolated using the genomic DNA extraction kit and used as template to yield cdtC genes by PCR. The amplifed cdtC genes were cloned into T-vector and cloned cdt C gene was then subcloned to pET28a expression vector. The pET28a-cdtC plasmid expressed in BL21 (DE3) Escherichia coli system. Diverse conditons were tested to opitimize the expression and purification of functional CdtC protein in E. coli. Results: In this study we reconstructed CdtC subunit of A. actinomycetemcomitans Y4 and comfirmed the recombinant CdtC expression by SDS-PAGE and Western Blotting. The expression level of the recombinant CdtC was about 2% of total bacterial proteins. Conclusion: The lab condition of procedure for the purification of functionally active recombinant CdtC protein is established. The active recombinant CdtC protein will serve to examine the role of CdtC proteins in the host recognition and enzyme activity of CDT and investigate the pathological process of A. actinomycetemcomitans in periodontal disease.

• 한국결정학회:학술대회논문집
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• 한국결정학회 2003년도 춘계학술연구발표회
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• pp.18-18
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• 2003

Thioredoxin (Trx) is a small redox protein that is ubiquitously distributed from achaes to human. In diverse organisms, the protein is involved in various physiological roles by acting as electron donor and regulators of transcription and apoptosis as well as antioxidants. Sequences of Trx within various species are 27～69% identical to that of E. coli and all Trx proteins have the same overall fold, which consists of central five β strands surrounded by four α helices. The N-terminal cysteine in WCGPC motif of Trx is redox sensitive and the motif is highly conserved. Compared with general cysteine, the N-terminal cysteine has low pKa value. The result leads to increased reduction activity of protein. Recently, novel thio.edoxin-related protein (TRP14) was found from rat brain. TRP14 acts as disulfide reductase like Trx1, and its redox potential and pKa are similar to those of Trx1. However, TRP14 takes up electrons from cytosolic thioredoxin reductase (TrxR1), not from the mitochondrial thioredoxin reductase (TrxR2). Biological roles of TES14 were reported to be involved in regulating TNF-α induced signaling pathways in different manner with Trx1. In depletion experiments, depletion of TRP14 increased TNF-α induced phosphorylation and degradation of IκBα more than the depletion Trx1 did. It also facilitated activation of JNK and p38 MAP kinase induced by TNF-α. Unlike Trx1, TRP14 shows neither interaction nor interference with ASK1. Here, we determined three-dimensional crystal structure of TRP14 by MAD method at 1.8Å. The structure reveals that the conserved cis-Pro (Pro90) and active site-W-C-X-X-C motif, which may be involved in substrate recognition similar to Trx1 , are located at the beginning position of strand β4 and helix α2, respectively. The TRP14 structure also shows that surface of TRP14 in the vicinity of the active site, which is surrounded by an extended flexible loop and an additional short a helix, is different from that of Trx1. In addition, the structure exhibits that TRP14 interact with a distinct target proteins compared with Trx1 and the binding may depend mainly on hydrophobic and charge interactions. Consequently, the structure supports biological data that the TRP14 is involved in regulating TNF-α induced signaling pathways in different manner with Trx1.

• 디지털융복합연구
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• 제18권9호
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• pp.191-198
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• 2020

본 연구에서는 화재대응능력 향상을 위한 화재모의훈련의 개선방안을 제안하기 위해 3개의 업무시설물을 대상으로 화재모의훈련을 실시하고 훈련 교보재의 활용 여부와 대응단계별 대응시간의 훈련평가 반영 여부에 따라 변화하는 훈련 참여자들의 대응자세 및 대응능력을 관찰 및 분석하였다. 분석결과 대다수의 훈련 참가자들은 훈련 교보재 활용과 대응단계별 대응시간이 훈련평가에 반영될 때 훈련 참여도, 대응자세 및 대응능력이 향상되는 것으로 분석되었다. 화재모의훈련을 실시할 경우 훈련 참여자들의 화재대응능력(화재통보 및 화재신고, 초기소화, 대피) 향상과 유지를 위해 화재발생 상황을 유사하게 구현할 수 있는 훈련 교보재를 다수 활용하고 각 대응단계별 목표시간을 설정한 후 이를 기반으로 정량적인 훈련평가를 하여야만 화재대응능력 향상과 향후 화재모의훈련의 개선 및 피드백에 유용한 지표로 활용될 수 있다는 결과를 도출하였다.

• 전자공학회논문지SC
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• 제42권6호
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• pp.37-48
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• 2005

내장형 시스템의 DC-DC 변환기나 배터리의 효율은 시스템을 구성하는 디바이스들의 종류 및 다양한 동작 패턴에 따른 전류의 시간적인 변화에 영향을 받는다. 이러한 특성은 DC-DC 변환기나 배터리의 효율을 분석하기 위해서는 다양한 부하를 가지는 실제 시스템을 구현, 응용프로그램을 수행하고, 배터리와 DC-DC 변환기를 포함한 전원 공급 회로를 연결하여 실제로 전력 소모를 측정할 것을 요구하지만, 이와 같이 실제 시스템을 구현한다는 것은 엄청난 개발 시간과 비용을 요구한다. 본 논문에서는 부하들의 전력 소모를 측정에 의해 얻어 저장하고 전원 공급 회로로부터 실제 시스템의 부하처럼 전력을 소모하도록 에뮬레이션 해줄 수 있는 시스템을 구현하였다. 구현한 부하 에뮬레이터(load emulator)는 측정한 전력 소모 프로파일의 양을 줄이기 위해 패턴 인식 후 압축하는 알고리즘을 사용하며, 저속 대용량 능동부하(active load)와 고속 소용량 능동부하들로 이루어진 비대칭(heterogeneous) 구조로 구현함으로써 전력 소모의 양이 많고 전력 소모가 신속하게 변하는 디지털 시스템의 부하를 에뮬레이션 할 수 있게 해준다. 구현한 부하 에뮬레이터의 성능을 평가하기 위해 하드디스크의 전력 소모를 측정 및 재생하여 비교하며, 부하 에뮬레이터의 응용으로써 부하의 전력 소모 패턴에 따른 DC-DC 변환기의 효율을 검토해 보았다.

• 보건교육건강증진학회지
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• 제17권2호
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• pp.183-205
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• 2000

This research was designed to compare the health condition and the quality of life of the elderly inpatients with those of the normal elderly people. The subjects of study were 482 elderly inpatients and 304 normal elderly people. The research was conducted in July and August using the instrument WHOQOL. The results of the research are as follows: For the level of health, it was recognized that male was healthier than female, having higher the education, living in the city rather than in the rural, keeping the normal life than being in the hospital. There were little difference in the past medical histories of the elderly inpatients and the normal elderly people. As to the diseases currently under the treatment, there were some difference between the elderly inpatients and the normal elderly people but the tendency was similar in the kinds and the frequencies. As to the recognition for the quality of life between the elderly inpatients and the normal elderly people based on the records reflected on each of the domains of WHOQOL, the normal elderly people more positively recognized in the overall quality of life and the following domains: physical, psychological, level of independence, and spirituality/religion/personal beliefs. The normal elderly people more positively recognized especially in the level of independence domain (mobility, activities of daily living, dependence on medication or treatments, working capacity). In the social relationships domain only the sexual activity was significant and the normal elderly people more positively recognized. The elderly inpatients showed the correlation of over 0.5 in the overall quality of life and the following domains: environment, social relationships, physical, psychological, level of independence. It was over 0.6 in the physical domain and the domains of psychological and level of independence. and the psychological domains of level of independence and social relationships. For the points of overall quality of life, it had no correlation with hospitalization but the health condition, residential district, occupation, and taste exerted a significant effect. As a result of separate analyses of the elderly inpatients and the normal elderly people, the health condition and the age only were the common variable which would exercise a significant effect. Besides, the primary factors which would exercise the quality of life were the occupation and taste for the elderly inpatients, and the residential district and source of income for the normal elderly people. In conclusion, it is first and foremost important to improve the standard of health for the overall quality of life for the elderly people, regardless of hospitalization. Therefore, a plan must be urgently drawn up for revitalization of the health promoting projects for the elderly people and the public health projects for the elderly people, and the investment must be increased for settlement of health problems of the elderly people.

• 수산경영론집
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• 제39권2호
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• pp.81-108
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• 2008

Since the declaration made by UN Convention on the Law of the Sea on EEZs, The open seas of Northeast Asia, considerd as a convention area, needed new agreements in conformity with the changes brought by the introduction of the Exclusive Economic Zone(EEZ) system. The Contracting Parties of these agreements set up their own EEZs, which extend certain ranges from their baselines, Fishing in the other party's EEZ is done based on mutual agreements, which take into account traditional fishing activity in the zones. Seperate fishries management systems, in accordance with the relevant legal status of the waters, are applied to individual overlapping areas: Middle Zone in the Bast Sea and the waters south of jeju Island, Interim Measure Zone in the Yellow Sea and East China Sea, and the Transitional Zone in the Yellow Sea. They decided to conclude fisheries agreements as the provisional agreement under Article 74(3) of the UN Convention before the delimitations of the EEZs to avoid the territorial disputes. China and Japan concluded the Fishries Agreement in the November 1997, allowing each coastal State 52 mile EEZ. it was followed by Korea and Japan in September 1998, reaching a final compromise. And also Korea and China came to a satisfactary settlement in November 1998. Fisheries agreements have been established between the three North-east Asian States, the agreement are all bilateral. That implies inefficient resource management on the overlapping waters of the three states, especially on the East China Sea. The Korea-Japan Fisheries Agreement and the China-Japan Fishery Agreement worked as governing rules in the North-east Asian seas before the establishment of EEZs (Exclusive Economic Zones). However the conclusion of the bilateral fishery agreements, Korea China and Japan have developed EEZs, and these three countries have competed for the exploitation of fisheries resources. Therefore, the issue of fisheries resource management was no longer a single countries' problem and emerged as a common issue facing these three countries. In recognition of the above-mentioned problem, it is needed for the construction of cooperative System fishery management in the North-east Asian seas. Therefore, cooperative measures should be establishied. The final goal of the construction of fisheries management cooperative system is to establish sustainable fisheries in the North-east Asian seas. However, there is a big difference in fisheries management tools, fishing gear, exploitation rate of species, etc. This implies that a careful approach should be taken in order to achieve the cooperative fisheries management among Korea, China and Japan. conclusionly, the Governments of Korea, China and Japan should complement three bilateral agreemens, and which they prepares to 'Fisheries Resource Restore Program' Between Korea, China and Japan in the adjacent waters south of Jeju Island.

• 말소리와 음성과학
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• 제12권2호
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• pp.51-61
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• 2020

본 연구의 목적은 음성의 청지각적 평가도구(GRBAS)와 음성관련 삶의 질(K-VRQOL) 척도를 통해 합창활동에 참여하는 여성 노인의 음성 특성과 음성관련 삶의 질을 비교하는 것이다. 연구 대상은 서울 및 부산 소재의 합창단에서 활동 중인 만 60세 이상의 여성 노인으로 총 77명이었다. 합창단은 참여 유형에 따라 합창단(Regular choir)과 찬양단(Church choir) 두 개의 집단으로 분류하였다. 청지각적 음성평가는 /a/ 모음을 발성하는 음성을 듣고 전문가가 청지각적 평가(GRBAS) 척도를 사용하여 평정하였다. 연구 결과, 합창활동 참여 유형에 따라 집단 간 차이를 비교했을 때 찬양단에서 활동하는 여성 노인에 비해 합창단에서 활동하는 여성 노인의 경우 주관적 음성 인식 수준에서 대화 시 음성 사용 만족도가 높은 것으로 나타났다. 또한, 음성관련 삶의 질(K-VRQOL) 척도의 신체 기능 영역에 해당하는 문항에서 만족도가 높은 것으로 분석되었다. 본 연구는 합창활동이 노년기 음성기능의 개선뿐 아니라 음성사용의 주관적 인식 수준을 향상시키는데 긍정적인 결과를 기대할 수 있을 것이라는 점을 확인하였으며, 노인 음성개선을 위한 체계적인 음악 중재 프로그램의 필요성을 시사하고 있다.