• The Journal of Korean Physical Therapy
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• 제24권4호
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• pp.282-289
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• 2012

Purpose: The purpose of this study is to analyze the home-stay disability's activity, as well as the participation domains and the environment factor domains of international classification of functioning (ICF), in order to examine the effect of the home visiting physical therapy and the disability's activity. Methods: A total of 211 home-stay disabled subjects with brain lesions or crippled disorder, living in 5 cities and districts of Jeollanam-do, underwent 90 minutes of home visiting physical therapy per week during a 6-month period, and using the ICF checklist, evaluated the subject's activity and participation domains and environmental factor domains. Results: The performance qualifier showed a significant statistical change in the movement, self-care, domestic life, interpersonal interactions and relationships, community, society and civic life domains (p<0.05); and the capacity qualifier showed a significant change only in the mobility domains in the before and after of the home visiting physical therapy (p<0.05). The barrier factor in the order of services, systems and policies domains, product and technology domains, and attitude domains it influenced significantly in the performance (p<0.05), and in the facilitator factor in the order of product and technology domains, support and relationships domains, services, systems and policies domains it influenced significantly in the performance of the disabled (p<0.05). Conclusion: The visiting physical therapy can help in the improvement of the activity and participation of the home-stay disabled subjects, and for the accurate evaluation of the home-stay disabled subjects, it is considered that an evaluation including various environmental factors, such as ICF, must be fulfilled.

• Molecules and Cells
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• 제19권2호
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• pp.246-255
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• 2005

Retroviral integrases insert viral DNA into target DNA. In this process they recognize their own DNA specifically via functional domains. In order to analyze these functional domains, we constructed six chimeric integrases by swapping domains between HIV-1 and HFV integrases, and two point mutants of HFV integrase. Chimeric integrases with the central domain of HIV-1 integrase had strand transfer and disintegration activities, in agreement with the idea that the central domain determines viral DNA specificity and has catalytic activity. On the other hand, chimeric integrases with the central domain of HFV integrase did not have any enzymatic activity apart from FFH that had weak disintegration activity, suggesting that the central domain of HFV integrase was defective catalytically or structurally. However, these inactive chimeras were efficiently complemented by the point mutants (D164A and E200A) of HFV integrase, indicating that the central domain of HFV integrase possesses potential enzymatic activity but is not able to recognize viral or target DNA without the help of its homologous N-terminal and C-terminal domains.

• BMB Reports
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• 제46권1호
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• pp.53-58
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• 2013

We constructed deletion mutants and seven point mutants by polymerase chain reaction to investigate the specificity of feline foamy virus integrase functional domains. Complementation reactions were performed for three enzymatic activities such as 3'-end processing, strand transfer, and disintegration. The complementation reactions with deletion mutants showed several activities for 3'-end processing and strand transfer. The conserved central domain and the combination of the N-terminal or C-terminal domains increased disintegration activity significantly. In the complementation reactions between deletion and point mutants, the combination between D107V and deletion mutants revealed 3'-end processing activities, but the combination with others did not have any activity, including strand transfer activities. Disintegration activity increased evenly, except the combination with glutamic acid 200. These results suggest that an intact central domain mediates enzymatic activities but fails to show these activities in the absence of the N-terminal or C-terminal domains.

• 한국초등과학교육학회지:초등과학교육
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• 제26권4호
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• pp.468-474
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• 2007

The purpose of this study was to examine the elementary pre-service teachers' interest in biological topics and activities. The instrument to survey the interest of them consists of 8 domains containing 27 topic items and 7 domains containing 12 activity items(three-point from 0 to 2 Likert scale). Data were collected from 108 pre-service teachers. The interest level in the topics was high, but the interest level in the activities was average. There was not different between interest levels of females and males in both topic and activity components (p > .05). Among the topics, the 'genetics' domain correlated strongly with the domains of 'nutrition and digestion', 'circulation', 'respiration' and 'nervous system', and the 'respiration' domain correlated with the 'circulation' domain(r >.450, p<.01). The correlation between the domains of 'out-of class experience activity' and 'visual information activity', and 'experimental performance following procedure' and 'discussing and debating'(r >.450, p<.01). This results suggested that the educational contents including learning materials and experiential activities be developed to activate the interest of elementary pre-service teachers in biology.

• Journal of Microbiology and Biotechnology
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• 제8권6호
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• pp.669-675
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• 1998

Taq DNA polymerase exhibits a sizable drawback compared to the other thermophilic DNA polymerases in that it demonstrates lower proof-reading activity due to the deficiency of 3'-5'exonuclease activity. A study was undertaken to improve the 3'-5' exonuclease activity in the PCR of Taq DNA polymerase. The three-dimensional structural alignment of the polymerase and 3'-5' exonuclease domains from the pol I family DNA polymerases explains why Taq DNA polymerase has just a background level of 3'-5'exonuclease activity. A comparison indicated that the two polymerase domains are very similar in primary and tertiary conformations, even though Taq DNA polymerase carries a much shorter 3'-5'exonuclease domain than that of E. coli DNA polymerase I. Those two polymerase domains were interchanged between Taq DNA polymerase and E. coli DNA polymerase I. The 3'-5' exonuclease domain from E. coli DNA polymerase I was separated and pasted into the polymerase domain of Taq DNA polymerase I, which resulted in a functional fusion-Stoffel fragment. The 3'-5'exonuclease activity of the fusion-Stoffel fragment increased up to 48% of the value of the Klenow fragment, while that of Taq DNA polymerase remained at 6.0% of the Klenow fragment.

• Safety and Health at Work
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• 제12권3호
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• pp.311-316
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• 2021

Background: The present study investigated the association between two domains of physical activity (occupational physical activity [OPA] and leisure-time physical activity [LTPA]) and sleep duration. Methods: We investigated 3,421 paid workers from the Korea National Health and Nutrition Examination Survey, 2014-2015. Sleep duration was categorized into three categories (short for less than 5 h, optimal for 5-9 h, and long for more than 9 h). OPA and LTPA were defined in terms of answers to relevant questions. Odds ratios were calculated for sleep duration according to each physical activity domain using multinomial logistic regression models. Results: There were 464 subjects (13.6%) who showed short sleep duration, and 169 subjects (4.9%) who showed long sleep duration. Prevalence of OPA and LTPA was higher in male workers than in female workers (for OPA: 3.67% and 1.76%, respectively, p = 0.0108; for LTPA: 16.14% and 6.07%, respectively, p < 0.0001). The odds ratio of OPA for long sleep duration in female workers was 3.35 (95% confidence interval, 1.37-8.21). Otherwise, LTPA was not associated with sleep duration in female paid workers, nor both physical activity domains in male paid workers. Conclusion: Female paid workers with work-related physical activity were at risk of oversleeping. These findings also suggested that physical activity has distinct associations with sleep duration according to the physical activity domains and sex.

• 대한간호학회지
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• 제30권6호
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• pp.1411-1426
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• 2000

A nursing activity classification for hospitalized patients was performed based on an article review regarding nursing definition and nursing activity classification system. The study was conducted as follows: 1) Taxonomy was developed by the research team through the Delphi process and review article. The taxonomy consists of four nursing processes, (assessment, diagnosis, intervention and evaluation) and twelve nursing activity domains space (resperation, nutrition, elimination, exercise/alignment maintenance, comfort, hygiene, safety, spiritual support, counseling/ education, medication, communication, patient and information management). 2) First, nursing activities of the intervention process were listed and then classified by the nursing process of assessment, diagnosis, intervention and evaluation. The list consists of twelve nursing activity domains and 136 nursing activities. 3) A pilot study was conducted in two hospitals to verify validity and appropriateness of nursing activities. 4) The content validity index, which was calculated by 6 clinical practice experts, was 0.95. Also, a nursing activity classification system should also be developed in the department of community nursing and home health care nursing.

• 보건교육건강증진학회지
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• 제29권3호
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• pp.23-34
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• 2012

Objectives: This study aims to compare the contents of physical activity questions of Korean National Health and Nutrition Examination Survey(KNHANES), and to propose suggestions for improvement. Methods: Questions measuring physical activity prevalence of adults in the KNHANES from KNHANES I(1998) to the first year of KNHANES V(2010) were compared. Results: The questions used in each survey showed some problems in consistency and appropriateness. Major issues identified are as follows. First, the questions in each survey were not consistent, and would be inappropriate to make time series comparisons. Second, the purpose of physical activity was not clear. Third, the examples of physical activities suggested in the questions were not appropriate. Fourth, the intensity of physical activity were not adequately described. Fifth, by excluding walking from the moderate-intensity physical activity category, the prevalence of moderate-intensity physical activity could be underestimated. Sixth, it is difficult to measure the total amount of physical activity performed in one day because answers from the other domains cannot be clearly differentiated. Conclusions: The physical activity questions in the KNHANES questionnaire should be improved so that they are consistent with the purpose of survey, the respondents can easily understand the contents of the questions, and the questions can measure all physical activity performed in the different domains.

• Asian-Australasian Journal of Animal Sciences
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• 제26권8호
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• pp.1144-1151
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• 2013

In this study, protein domains with cellulase activity in goat rumen microbes were investigated using metagenomic and bioinformatic analyses. After the complete genome of goat rumen microbes was obtained using a shotgun sequencing method, 217,892,109 pair reads were filtered, including only those with 70% identity, 100-bp matches, and thresholds below $E^{-10}$ using METAIDBA. These filtered contigs were assembled and annotated using blastN against the NCBI nucleotide database. As a result, a microbial community structure with 1431 species was analyzed, among which Prevotella ruminicola 23 bacteria and Butyrivibrio proteoclasticus B316 were the dominant groups. In parallel, 201 sequences related with cellulase activities (EC.3.2.1.4) were obtained through blast searches using the enzyme.dat file provided by the NCBI database. After translating the nucleotide sequence into a protein sequence using Interproscan, 28 protein domains with cellulase activity were identified using the HMMER package with threshold E values below $10^{-5}$. Cellulase activity protein domain profiling showed that the major protein domains such as lipase GDSL, cellulase, and Glyco hydro 10 were present in bacterial species with strong cellulase activities. Furthermore, correlation plots clearly displayed the strong positive correlation between some protein domain groups, which was indicative of microbial adaption in the goat rumen based on feeding habits. This is the first metagenomic analysis of cellulase activity protein domains using bioinformatics from the goat rumen.

• 아동학회지
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• 제24권6호
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• pp.47-60
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• 2003

This study analyzed the pattern of preschool children's abilities based on Project Spectrum and compared the relationship between intelligence domains with working styles of children. Generally, children showed both strengths and weaknesses in specific Spectrum domains, but some children had only strengths and others had only weaknesses without strengths. It appeared that working style does not relate to children's strong domains, but individual disposition was reflected by characteristics of the task. During both individual and group spectrum activities, children with strengths were more "easily engaged in activity", "confident", and "focused" than those without strong intelligence domains. During individual activities, the "reflective" domain showed children with strengths in intelligence domains are more reflective than those without strengths.