• Title/Summary/Keyword: active transport

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Changes on the Methylmercury-induced Cytotoxicity by Control of Cell Membrane Transport System (세포막 물질수송계의 조절에 의한 유기수은의 세포독성 변화에 대한 연구)

  • 염정호;고대하;김준연;김남송
    • Journal of Environmental Health Sciences
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    • v.26 no.4
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    • pp.88-96
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    • 2000
  • The aim of the current study was to evaluate the transport system in EMT-6 cell for the uptake of the methylmercury(MeHg). Several inhibitors ere used to test used to test which potential transport system might be involoved in MeHg uptake. Probenecid was used to test the organic transport system, valinomycin for testing the effect of the membrane potential, cytochalasin B for testing the facilitated diffusive D-glucose transport system and colchicine for testing the microtubule system. Ouabain for evaluating active transport system, 4',4-diisothiocyano-2',2-stilbenedisulfonic acid(DIDS) the Cl- ion transport system and verapamil for the $Ca^{2+}$ transprot system. Significantly, MeHg decreased the synthesis of nitric oxcide(NO) and intracellular ATP in ENT-6 cells. In the condition of ouabain containing with MeHg decreased the production of NO and intracelluar ATP. In the treatment of inhibitors, ouabain showed protective effect against cytotoxicity of MeHg but ather inhibitors not showed protective effects. The protective effects of ouabain against the cytotoxicity of MeHg deoended on the concentration of added ouabain to the culture medium for MET-6 cells. These result showed that the uptake of MeHg might be involved in the active transport system. Active transports system seems to share similarities with the transport systems for the uptake of MeHg when using MeHg and MeHg-glutathione complex.x.

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Carrier-Mediated Active Transport Models by Affinity-Switching Strategy

  • Lee, Sung-Kil;Araki, Koji
    • Proceedings of the Membrane Society of Korea Conference
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    • 1996.04a
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    • pp.5-10
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    • 1996
  • Active transport is one of the essential function of biological membrane transport systems, in which substrates are transported from lower to higher concentration side against their concentration gradient. In this article, we describe photo- and pH-induced active transport models by designing the functional carriers based on the affinity-switching strategy.

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Development of Transport Parameters affecting on the Removal of Micro Organic Compounds such as Disinfection By-Products and Pharmaceutically Active Compounds by Low-Pressure Nanofiltration

  • Oh, Jeong-Ik;Yamamoto, Kazuo
    • Environmental Engineering Research
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    • v.14 no.2
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    • pp.126-133
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    • 2009
  • This study investigated the removal characteristics of various micro organic compounds by low-pressure nanofiltration membranes comprised of disinfection by products and pharmaceutically active compounds. The experimental removal of micro organic compounds by low-pressure nanofiltration membranes was compared with the transport model calculations, which consist of diffusion and convection terms including steric hindrance factor. The selected molecule from the disinfection byproducts and pharmaceutical active compounds showed a much lower removal than polysac-charides with a similar molecular size. However,the difference between model calculation and experimental removal of disinfection by-products and pharmaceutically active compounds could be corrected. The correlation of Ks with solute radius was further considered to clarity transport phenomena of micro organic solutes through nanofiltration membranes.

Measuring stress responses using active biotelemetry in cattle II. Fluid administration for reducing transport stress (Active biotelemetry를 이용한 젖소의 스트레스 반응 측정: II. 수송 스트레스 감소를 위한 수액투여 효과)

  • Lee, Su-han;Lee, Byeong-han;Lim, Joa-jin;Kim, Jin-young;Lee, Dong-hee;Kim, Jae-kyung;Choe, Nong-hoon;Jeong, Soon-wuk;Chung, Byung-hyun
    • Korean Journal of Veterinary Research
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    • v.41 no.4
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    • pp.603-609
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    • 2001
  • A transport stress is one of the main causes of economic losses and physiological dysfunction. The present study has been performed to suggest a method to decrease the adverse effects above mentioned from transport. The groups were prepared as follows; (1) Control group : 4 cattle transported for 5 hrs (274 km) without any treatment, (2) Treatment group : 4 cattle treated with electrolyte-mineral solution (I.V.) at 1 hr before the enrollment of transport under same experimental condition with Control group. The blood specimens were collected at 1 hr before transport, 2.3 hrs (135 km) and 5 hrs (274 km) after the enrollment of transport, and 1, 6 and 18 hrs after fulfillment of transport. The collected blood specimens were analyzed for cortisol and epinephrine. Core temperature and heart rate were measured with active biotelemetry in every 30 minutes from 0.5 hr before the start of transport to 18 hrs after the end of transport. In results, the level of cortisol considerably increased to the peak either in Control group ($5.3{\pm}1.3{\mu}g/d{\ell}$) and in Treatment group ($4.0{\pm}2.6{\mu}g/d{\ell}$) at 2.3 hrs in transport. The concentration of epinephrine of Treatment group had been higher than that of Control group from the start of transport to 18 hrs after the fulfillment of transport. Particularly there was the biggest gap between Control and Treatment groups, $424.0{\pm}194.1pg/m{\ell}$ and $209.1{\pm}65.1pg/m{\ell}$ respectively, at 6 hrs after the end of transport. The heart rates were considerably increased either in Control group ($81.5{\pm}18.5$ to $126.3{\pm}7.8beats/min$) and in Treatment group ($114.3{\pm}14.4$ to $140.8{\pm}22.4beats/min$) with the enrollment of transport. These results indicate that the concentration of cortisol and the heart rate were pertinent to cognitive parameters to evaluate physiological responses against stress such as transport. In addition, the intravenous administration of electrolyte-mineral solution could be suggested as the method to decrease the adverse effects from a transport stress.

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Transport Coefficients across Charged Mosaic Membrane

  • Yang, Wong-Kang
    • Bulletin of the Korean Chemical Society
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    • v.25 no.5
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    • pp.665-667
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    • 2004
  • In previous studies, charged mosaic membranes having two different fixed charges in the membrane matrix indicated unique transport behavior such as preferential material transport. In this study, the composite charged mosaic membrane endurable to mechanical pressure in practical application was investigated from the same aspect of solute and solvent transport as before. Lp and ${\omega}$ estimated by taking account of active layer thickness were satisfactorily consistent with those in mosaic membrane without reinforcement. On the other hand, the reflection coefficient s indicated the negative value that suggests preferential material transport.

The Effect of LSC/GDC (50 : 50 vol%) Active Layers on Oxygen Transport Properties of LSCF/GDC (20 : 80 vol%) Dual-phase Membrane (LSC/GDC (50 : 50 vol%) 활성층이 LSCF/GDC (20 : 80 vol%) 복합 분리막의 산소투과 거동에 미치는 영향)

  • Cha, Da-Som;Yoo, Chung-Yul;Joo, Jong Hoon;Yu, Ji Haeng;Han, Moon-Hee;Cho, Churl-Hee
    • Membrane Journal
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    • v.24 no.5
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    • pp.367-374
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    • 2014
  • In the present study, disc-type LSCF/GDC (20 : 80 vol%) dual-phase membranes having porous LSC/GDC (50 : 50 vol%) active layers were prepared and effect of active layers on oxygen ion transport behavior was investigated. Introduction of active layers improved drastically oxygen flux due to enhanced electron conductivity and oxygen surface exchange activity. As firing temperature of active layer increased from $900^{\circ}C$ to $1000^{\circ}C$, oxygen flux increased due to improved contact between membrane and active layer or between grains of active layer. The enhanced contact would improve oxygen ion and electron transports from active layer to membrane. Also, as thickness of active layer increased from 10 to $20{\mu}m$, oxygen flux decreased since thick active layer rather prevented oxygen molecules diffusing through the pores. And, STF infiltration improved oxygen flux due to enhanced oxygen reduction reaction rate. The experimental data announces that coating and property control of active layer is an effective method to improve oxygen flux of dual-phase oxygen transport membrane.

Enhancement of Optical Transport Technology for 5G Mobile Fronthaul (5G 모바일 프론트홀 광전송 기술 동향)

  • Chang, S.H.;Hwang, I.K.;Kang, H.S.;Lee, S.W.;Lee, J.K.
    • Electronics and Telecommunications Trends
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    • v.37 no.3
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    • pp.23-32
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    • 2022
  • This paper provides an overview of optical transport technology for 5G mobile fronthaul. The configuration of fronthaul network is classified with dedicated fiber, passive WDM(Wavelength Division Multiplexing), active WDM, and semi-active WDM, which has its own advantages and drawbacks. Various WDM technology is applied for fronthaul transport, depending on the wavelength bands, required number of wavelength channels, configuration of fronthaul network, etc. In order to meet the increasing transport capacity, a 50/100 Gbps optical transceiver will be used in place of the present 10/25 Gbps technology. Trends will be continued to enhance the flexibility and reliability of the fronthaul optical network supporting highly advanced 5G mobile services.

On the implementation of Taper slot array antenna structure (Taper 슬롯구조배열 안테나 구현)

  • Lee, Cheon-Hee;Kim, Ho-Jun;Kwak, Kyung-Sup
    • The Journal of The Korea Institute of Intelligent Transport Systems
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    • v.13 no.1
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    • pp.127-134
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    • 2014
  • X-Band taper slot-typed active phased array antenna is studied and designed. Through the simulated and measured performances, it is confirmed that both of active reflection coefficient and active radiation pattern of the designed phased array antenna are agreed well with those of the prototype manufactured one. From this study, the proposed antenna structure is matched to the design target of characteristics of antenna's broadband beam.

Performance Analysis of Fuel Cell by Controlling Active Layer Thickness of Catalyst (촉매 활성층 두께 제어를 통한 연료전지 성능 해석)

  • Kim, H.G.
    • Transactions of the Korean Society of Machine Tool Engineers
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    • v.16 no.3
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    • pp.133-140
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    • 2007
  • A 2-D model of fluid flow, mass transport and electrochemistry is analysed to examine the effect of current density at the current collector depending on active layer thickness of catlyst in polymer elecrolyte fuel cells. The finite element method is used to solve the continuity, potential and Maxwell-Stefan equations in the flow channel and gas diffusion electrode regions. For the material behavior of electrode reactions in the active catalyst layers, the agglomerate model is implemented to solve the diffusion-reaction problem. The calculated model results are described and compared with the different thickness of active catalyst layers. The significance of the results is discussed in the viewpoint of the current collecting capabilities as well as mass transportation phenomena, which is inferred that the mass transport of reactants dictates the efficiency of the electrode in the present analysis.

The Induction Time of Sucrose Active Transport System during the Phloem Cell Development in Suspension Cultures of Streptantus tortus Cotyledon (Streptanthus tortus 자엽의 배양세포에서 사부세포 발달동안 Sucrose 능동수송계의 유도 시기)

  • Cho, Bong-Heuy
    • Journal of Plant Biotechnology
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    • v.31 no.2
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    • pp.169-173
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    • 2004
  • Parenchyma cells of Streptanthus tortus suspension cultures possessed the different transport system for aldose-formed D-glucose and for ketose-formed D-fructose. $K_{m}$ value for D-glucose and D-fructose were 0.28mM and 15.02mM, respectively. $K_{m}$ value of D-mannose was 0.44 mM which is similar to the D-glucose transport system, but D-mannose was transported also through its own special uptake system. Parenchyma cells possessed the transport system of L-glucose, but the function of L-glucose was not known at all. Protoplast of parenchyma cells possessed only the monosugars transport system, but didn't possess the disugars, sucrose transport system. Early developing phloem protoplasts possessed glucose and sucrose transport system at the same time. On the contrary, in the complete developed phloem cells disappeared preexisted glucose transport system in the parenchyma cells, only new induced sucrose transport system existed.ted.