• ETRI Journal
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• v.10 no.4
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• pp.118-126
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• 1988

A numerical analysis was performed for the flow field in the vertical channels consist of dummy cards and active cards to define the hydrodynamic role of dummy card which is often installed in electronic equipment between active cards to control the cooling air distribution. For a given velocity profile at the inlet and a pressure-based boundary condition at the outlet of the computation domain, the percentage of the flow rate distribution through active channel and dummy channel formed by an active card and dummy card, respectively, were investigated. As a result of present analysis, the pecentage of flow rate through active channel increases quadraticaly with the increase of the ratio of the height of barrier to the width of the dummy channel.

• Proceedings of the KIEE Conference
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• 2003.04a
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• pp.292-295
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• 2003

Conventional methods in time / frequency domain for evaluating the compensation performance of active power filters are not provided easy solutions. So, the authors have previously proposed 3-D current coordinates which is composed into active component, fundamental reactive component and distorted component of nonlinear loads current. This method has excellent performance, but can not evaluate the characteristics of nonlinear load current whether it is inductive or capacitive. Therefore, To overcome problems mentioned previously, this paper deals with the simple Average power theory and the expanded 3-D current coordinates for evaluating the compensation performance of active power filters. To confirm the validity, active power filters simulator is developed using C-language. From the simulation, results are discuss their utility.

• Journal of Information Technology Applications and Management
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• v.27 no.1
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• pp.125-145
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• 2020

We explore technology ecosystem of smart surgical medical system by analyzing patent data applied for in Korea and Japan. First, a review of trends of patent application by country/technological domain show that there exist a minority of technology domains focused on R&D, which represent their trends have been increasingly active. Also, while a number of Japanese firms mainly consist of the patent market of Japan, in case of the Korean market, a few universities, SMEs, and foreign firms are found to be the main applicants. As a result of the network analysis with the links as the relations of co-patenting, the relationships, which are active of convergence and knowledge spillover among the heterogeneous technology domains within each market, as well as the technology domains, which are the most active in international cooperation among each homogeneous domain, could get derived and visualized in the ecosystem. In addition, the technology domains in each patent market with leading locations, roles, and influence in the network can also be identified through the centrality analysis. In this study, the analysis for technology competitiveness are carried out focusing on patent activity and patent impact. The results denote that across all domains, the Japanese market may possess higher patent activity and patent impact compared to the Korean market. In consequence, we derive the position map for comparison by country and technology domain from a perspective considering comprehensively the multi-dimensional attributes based on the results of both network analysis and technology competitiveness.

• The Journal of the Acoustical Society of Korea
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• v.37 no.6
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• pp.489-498
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• 2018

In this paper, a target detection method based on beamspace-domain multichannel nonnegative matrix factorization is studied when an echo of continuous-wave ping is received from a low-Doppler target in reverberant environment. If the receiver of the continuous-wave active sonar moves, the frequency range of the reverberation is broadened due to the Doppler effect, so the low-Doppler target echo is interfered by the reverberation in this case. The developed algorithm analyzes the multichannel spectrogram of the received signal into frequency bases, time bases, and beamformer gains using the beamspace-domain multichannel nonnnegative matrix factorization, then the algorithm estimates the frequency, time, and bearing of target echo by choosing a proper basis. To analyze the performance of the developed algorithm, simulations were performed in various signal-to-reverberation conditions. The results show that the proposed algorithm can estimate the frequency, time, and bearing, but the performance was degraded in the low signal-to-reverberation condition. It is expected that modifying the selection algorithm of the target echo basis can enhance the performance according to the simulation results.

• Molecules and Cells
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• v.22 no.1
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• pp.13-20
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• 2006

Hepatitis C virus (HCV) non-structural protein 5A protein (NS5A), which consists of three functional domains, is involved in regulating viral replication, interferon resistance, and apoptosis. Recently, the three-dimensional structure of the domain 1 was determined. However, currently the molecular basis for the domains 2 and 3 of HCV NS5A is yet to be defined. Toward this end, we expressed, purified the domain 2 of the NS5A (NS5A-D2), and then performed biochemical and structural studies. The purified domain 2 was active and was able to bind NS5B and PKR, biological partners of NS5A. The results from gel filtration, CD analysis, 1D $^1H$ NMR and 2D $^1H-^{15}N$ heteronuclear single quantum correlation (HSQC) spectroscopy indicate that the domain 2 of NS5A appears to be flexible and disordered.

• Journal of KSNVE
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• v.7 no.3
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• pp.427-437
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• 1997

Mean active intensity based active control for the cancellation of radiated noise out of the duct exit is studied. The active intensity control strategy is drerived based on the relation of the exterior sound field out of the duct termination and interior sound field of the duct. One of the characteristics of this control strategy is that the control performance can be maintained regardless of the sensor loction, compared with the conventional local pressure control methods at either interior downstream or exterior field positions. It is also suggested that the digital filtering for the active intensity control can be achieved by time-domain filtered-x LMP (Lest-Mean-Product) adaptive algorithm. Experiments for an open-ended duct are performed to compare the active intensity control performance with conventional pressure control one. Active control experiment of local sound pressure is conducted by widely used filtered-x LMS adaptive Algorithm and active intensity control implementaion uses the derived filter d-x LMP algorithm. It is shown that the exterior sound fileds was much better observable by sensing of the active intensity than by just sound pressure. It is also demonstrated that the global control performance of external field by acoustic intensity is superior to the conventional sound pressure control performance.

• Journal of Microbiology and Biotechnology
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• v.26 no.10
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• pp.1701-1707
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• 2016

Lipoxygenase (LOX) is an industrial enzyme with wide applications in food and pharmaceutical industries. The available structure information indicates that eukaryotic LOXs consist of N terminus β-barrel and C terminus catalytic domains. However, the latest crystal structure of Pseudomonas aeruginosa LOX shows it is significantly different from those of eukaryotic LOXs, including the N-terminal helix domain. In this paper, the functions of this N-terminal helix domain in the soluble expression and catalysis of P. aeruginosa LOX were analyzed. Genetic truncation of this helix domain resulted in an insoluble P. aeruginosa LOX mutant. The active C-terminal domain was obtained by dispase digestion of the P. aeruginosa LOX derivative containing the genetically introduced dispase recognition sites. This functional C-terminal domain showed raised substrate affinity but reduced catalytic activity and thermostability. Crystal structure analyses demonstrate that the broken polar contacts connecting the two domains and the exposed hydrophobic substrate binding pocket may contribute to the insoluble expression of the C terminus domain and the changes in the enzyme properties. Our data suggest that the N terminus domain of P. aeruginosa LOX is required for its soluble expression in E. coli, which is different from that of the eukaryotic LOXs. Besides this, this N-terminal domain is not necessary for catalysis but shows positive effects on the enzyme properties. The results presented here provide new and valuable information on the functions of the N terminus helix domain of P. aeruginosa LOX and further improvement of its enzyme properties by molecular modification.

• BMB Reports
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• v.40 no.1
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• pp.58-64
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• 2007

Similar to the other known structures of Bacillus thuringiensis Cry $\delta$-endotoxins, the crystal structure of the 65-kDa activated Cry4Ba toxin comprises three domains which are, from the N- to C-terminus, a bundle of $\alpha$-helices, a three-$\beta$-sheet domain, and a $\beta$-sandwich. To investigate the properties of the C-terminal domain III in isolation from the rest of the toxin, the cloned Cry4Ba-domain III was over-expressed as a 21-kDa soluble protein in Escherichia coli, which cross-reacted with anti-Cry4Ba domain III monoclonal antibody. A highly-purified domain III was obtained in a monomeric form by ion-exchange and size-exclusion FPLC. Circular dichroism spectroscopy indicated that the isolated domain III fragment distinctly exists as a $\beta$-sheet structure, corresponding to the domain III structure embodied in the Cry4Ba crystal structure. In vitro binding analysis via immuno-histochemical assay revealed that the Cry4Ba-domain III protein was able to bind to the apical microvilli of the susceptible Stegomyia aegypti larval midguts, albeit at lower-binding activity when compared with the full-length active toxin. These results demonstrate for the first time that the C-terminal domain III of the Cry4Ba mosquito-larvicidal protein, which can be isolated as a native folded monomer, conceivably participates in toxin-receptor recognition.

• Proceedings of the Korean Society for Applied Microbiology Conference
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• 2002.10a
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• pp.18-25
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• 2002

The pikromycin biosynthetic system in Streptomyces venezuleae is unique for its ability to produce two groups of antibiotics that include the 12-membered ring macrolides methymycin and neomethymycin, and the 14-membered ring macrolides narbomycin and pikromycin. The metabolic pathway also contains two post polyketide-modification enzymes, a glycosyltransferase and P450 hydroxylase that have unusually broad substrate specificities. In order to explore further the substrate flexibility of these enzymes a series of hybrid polyketide synthases were constructed and their metabolic products characterized. The plasmid-based replacement of the multifunctional protein subunits of the pikromycin PKS in S. venezuelae by the corresponding subunits from heterologous modular PKSs resulted in recombinant strains that produce both 12- and 14-membered ring macrolactones with predicted structural alterations. In all cases, novel macrolactones were produced and further modified by the DesVII glycosyltransferase and PikC hydroxylase leading to biologically active macrolide structures. These results demonstrate that hybrid PKSs in S. venezuelae can produce a multiplicity of new macrolactones that are modified further by the highly flexible DesVII glycosyltransferase and PikC hydroxylase tailoring enzymes. This work demonstrates the unique capacity of the S. venezuelae pikromycin pathway to expand the toolbox of combinatorial biosynthesis and to accelerate the creation of novel biologically active natural products. The polyketide backbone of rifamycin B is assembled through successive condensation and ${\beta}$-carbonyl processing of the extender units by the modular rifamycin PKS. The eighth module, in the RifD protein, contains nonfunctional DH domain and functional KR domain, which specify the reduction of the ${\beta}$-carbonyl group resulting in the C-21 bydroxyl of rifamycin B. A four amino acid substitution and one amino acid deletion were introduced in the putative NADPH binding motif in the proposed KR domain encoded by rifD. This strategy of mutation was based on the amino acid sequences of the corresponding motif of the KR domain of module 3 in the RifA protein, which is believed dysfunctional, so as to introduce a minimum alteration and retain the reading frame intact, yet ensure loss of function. The resulting strain produces linear polyketides, from tetraketide to octaketide, which are also produced by a rifD disrupted mutant as a consequence of premature termination of polyketide assembly. Much of the structural diversity within the polyketide superfamily of natural products is due to the ability of PKSs to vary the reduction level of every other alternate carbon atom in the backbone. Thus, the ability to introduce heterologous reductive segments such as ketoreductase (KR), dehydratase (DH), and enoylreductase (ER) into modules that naturally lack these activities would increase the power of the combinatorial biosynthetic toolbox. The dehydratase domain of module 7 of the rifamycin PKS, which is predicted to be nonfunctional in view of the sequence of the apparent active site, was replaced with its functional homolog from module 7 of rapamycin-producing polyketide synthase. The resulting mutant strain behaved like a rifC disrupted mutant, i.e., it accumulated the heptaketide intermediate and its precursors. This result points out a major difficulty we have encountered with all the Amycolatopsis mediterranei strain containing hybrid polyketide synthases: all the engineered strains prepared so far accumulate a plethora of products derived from the polyketide chain assembly intermediates as major products instead of just analogs of rifamycin B or its ansamycin precursors.

• Hwankyungkyoyuk
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• v.12 no.1
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• pp.150-171
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• 1999

This study is aimed to systematize the contents of environmental education in the home education and to develop the environmental education. For this, this study firstly examined the characteristics and goals of home education and its relation to environmental education. Secondly, in the paradigm of environmental education this study analyzed the goals and contents of environmental education in the domain of home education which is included in the practicum subject of the 7th curriculum. Thirdly, this study examined the effective teaching and learning methods for home education and the considerations in organization of textbook contents which reflect these teaching and learning methods. Finally, this study suggested an example of textbooks which reflect all these considerations. It has been found out that: (1) the home education is based on the recognition of environmental problems related to home life, (2) the home education explains the environmental problems, and (3) the home education has its goals of acquring active attitudes and skills to solve these problems. In the teaching contents, the home education was analyzed to have the following concerning environmental education: the utilization of resources for food, clothes, and housing and home life, that is, the domain of ‘environmental sanitation’ related to food ingestion and sanitary life, the domain of ‘sound consumption life’ related to the utilization of resources and their consumption, and the domain of ‘environmental pollution’ on the prevention of every kind of pollutants in the home life. However, the environmental education in the home education according to the 7th educational curriculum has the distinctiveness from the contents of environmental education which were emphasized in the past home education. The distinctiveness are as following: (1)the resources matter is dealt with in the aspect of recycling various resouces from home life, (2)the prevention or reduction of pollutants in life which take the considerable part of environmental pollution is emphasized, (3)children's sensitivity In environment is emphasized to be developed, and (4)the importance of life is emphasized to be taught.