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Molecular and Structural Characterization of the Domain 2 of Hepatitis C Virus Non-structural Protein 5A  

Liang, Yu (Division of Structural and Computational Biology, School of Biological Sciences, Nanyang Technological University)
Kang, Cong Bao (Division of Structural and Computational Biology, School of Biological Sciences, Nanyang Technological University)
Yoon, Ho Sup (Division of Structural and Computational Biology, School of Biological Sciences, Nanyang Technological University)
Publication Information
Molecules and Cells / v.22, no.1, 2006 , pp. 13-20 More about this Journal
Abstract
Hepatitis C virus (HCV) non-structural protein 5A protein (NS5A), which consists of three functional domains, is involved in regulating viral replication, interferon resistance, and apoptosis. Recently, the three-dimensional structure of the domain 1 was determined. However, currently the molecular basis for the domains 2 and 3 of HCV NS5A is yet to be defined. Toward this end, we expressed, purified the domain 2 of the NS5A (NS5A-D2), and then performed biochemical and structural studies. The purified domain 2 was active and was able to bind NS5B and PKR, biological partners of NS5A. The results from gel filtration, CD analysis, 1D $^1H$ NMR and 2D $^1H-^{15}N$ heteronuclear single quantum correlation (HSQC) spectroscopy indicate that the domain 2 of NS5A appears to be flexible and disordered.
Keywords
Expression and Purification; Hepatitis C Virus NS5A; NMR; NS5A Domain 2; NS5B;
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
Times Cited By Web Of Science : 14  (Related Records In Web of Science)
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