• Title/Summary/Keyword: acp

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Development of the Interface Module for an Effective Application of a Digital Mockup

  • Song, Tai-Gil;Kim, Sung-Hyun;Lim, Gwang-Mook;Yoon, Ji-Sup;Lee, Sang-Ho
    • 제어로봇시스템학회:학술대회논문집
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    • 2005.06a
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    • pp.2407-2409
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    • 2005
  • As the cumulative amount of spent fuel increases, the reliable and effective management of the spent fuel has become a world-wide mission. For this mission, KAERI is developing the Advanced Spent Fuel Conditioning Process (ACP) as a pre-disposal treatment process for spent fuel. Conventional approach to the development of the process and the remote operation technology is to fabricate the process equipment on the same scale as the real environment and demonstrate the remote handling operation using simulated fuel called a mock-up test. But this mock-up test is expensive and time consuming, since the design may need to be modified and the equipment fabricated again to account for the problems found during a testing. To deal with this problem, we developed a digital mockup for the ACP. Also, for an effective utilization of the digital mockup, we developed user interface modules such as the data acquisition and display module and the external input device interface module. The result of this implementation shows that a continuous motion of the manipulator using the external device interface can be represented easily and the information display screens responded well to the simulation situation.

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The Effects of Acute Osmotic Stress on Innate Immunity of Nile Tilapia (Oreochromis niloticus)

  • Choi, Sang-Hoon;Park, Kwan-Ha
    • Fisheries and Aquatic Sciences
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    • v.13 no.4
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    • pp.343-349
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    • 2010
  • The effects of osmotic stress on the non-specific immune response of Nile tilapia, Oreochromis niloticus, were investigated. Osmoregulatory mechanism of tilapia has been studied, but less information is available about innate immune response of O. niloticus faced with hyperosmolality. Acute osmotic stress was elicited by transferring tilapia from freshwater (FW) to 24 psu seawater (SW). Non-specific immune parameters including lysozyme activities of plasma and head kidney (HK), alternative complement pathway (ACP) activity in plasma, phagocytic capacities of spleen and HK immune cells, and respiratory burst activity of immune cells in both HK and spleen were analyzed. Lysozyme activities were increased at 1 h and 30 h after transfer to SW, but decreased at 10 h after SW transfer. Conversely, ACP activity increased 10 h after SW transfer. Phagocytic capacity increased slightly at 1 h and 5 h after SW transfer, and respiratory burst activity showed an increase in superoxide release at 10 h after SW transfer. Taken together, these results indicate that the exposure of tilapia to hyperosmotic conditions has immunostimulatory effects on cellular and humoral immune reactions.

Radiation Dose Evaluation for Metallization Process Facility of Spent Fuel (사용후핵연료 금속전환공정시설의 방사선환경영향평가)

  • 국동학;정원명;구정회;조일제;이은표;유길성
    • Proceedings of the Korean Radioactive Waste Society Conference
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    • 2003.11a
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    • pp.596-600
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    • 2003
  • The Advanced spent fuel Conditioning Process(ACP) is under development for the effective management of spent fuel which had been generated in nuclear plants. The ACP needs a hot cell where most operations will be peformed. To give priority to the environment safety, radiation doses evaluation for the radioactive nuclides were preliminarily peformed in both normal operation and accident case. The evaluation result shows a safe margin for regulation limits and SAR limit of IMEF where this facility will be constructed.

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Comparison of enzyme cytochemical activities between rosetted cells in peripheral blood of Korean native cattle (한우(韓牛) 말초혈액내(末梢血液內) rosette 형성세포(形成細胞)의 효소화학적(酵素化學的) 염색성(染色性) 비교(比較))

  • Kim, Nyeun-su;Song, Hee-jong;Kim, Sun-jae;Seo, Ye-won
    • Korean Journal of Veterinary Research
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    • v.37 no.1
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    • pp.129-135
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    • 1997
  • Peripheral blood mononuclear cells(PBMNC) of Korean native cattle rosetted with Korean goat erythrocytes(KGRBC) and blood monocytes were evaluated for four cytochemical reactions such as acid phosphatase(ACP), alkaline phosphatase-anti-boby(ALP-Ab), ${\alpha}$-naphthyl butyrate esterase(${\alpha}$-NBE) and peroxidase. The results obtained were as follows; In rosetted cells, the positivities of ACP in E AET-DeX, EA and EAC were 70.3%, 22.4% and 25.2%, those of ${\alpha}$-NB were 27.4%, 44.2% and 79.8%, and those of ALP-Ab were 9.5%, 88.3% and 91.5%, respectively. Whereas, the positivity for Peroxidase in monocytes was 100%. In non-rosetted (remained) cells, the positivities of ACP in E AET+DeX. EA and EAC were 41.4%, 57.2% and 61.9%, those of ${\alpha}$-NB were 38.6%, 16.5% and 18.9% and those of ALP-Ab were 98.2%, 5.3% and 6.3%, in order.

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Multiplex PCR Detection of the MON1445, MON15985, MON88913, and LLcotton25 Varieties of GM Cotton

  • Kim, Jae-Hwan;Kim, Sun-A;Seo, Young-Ju;Lee, Woo-Young;Park, Sun-Hee;Kim, Hae-Yeong
    • Food Science and Biotechnology
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    • v.17 no.4
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    • pp.829-832
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    • 2008
  • A multiplex polymerase chain reaction (PCR) method was developed to simultaneously detect 4 varieties of genetically modified (GM) cotton. The event-specific primers were used to distinguish the 4 varieties of GM cotton (MON1445, MON15985, MON88913, and LLcotton25) using multiplex PCR. The acyl carrier protein 1 (Acp1) gene was used as an endogenous reference gene of cotton in the PCR detection. The primer pair Acp1-AF/AR containing a 99 bp amplicon was used to amplify the Acp1 gene and no amplified product was observed in any of the 13 different plants used as templates. This multiplex PCR method allowed for the detection of event-specific targets in a genomic DNA mixture of up to 1% GM cotton containing MON1445, MON15985, MON88913, and LLcotton25.

Differentially Expressed mRNA Profiles between Immature Germinal Vesicle(GV) and Mature Metaphase II(MII) Mouse Oocytes (미성숙 난자와 성숙 난자에서 서로 다르게 발현하는 유전자에 관한 연구)

  • Yoon Se-Jin;Chung Hyung-Min;Cha Kwang-Yul;Kim Nam-Hyung;Lee Kyung-Ah
    • Development and Reproduction
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    • v.8 no.1
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    • pp.35-42
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    • 2004
  • Oocyte maturation refers to the process that prophase I arrested germinal vesicle(GV) drives the progression of meiosis to metaphase II(MII) to have the capacity for fertilization and embryo development. To better understand the molecular mechanism(s) involved in oocyte maturation, we identified differentially expressed genes(DEGs) between GV and MII mouse oocytes using a new innovative annealing control primer (ACP) technology. Using 20 ACPs, we successfully cloned 32 DEGs between GV and Mll oocytes, and 26 out of these 32 DEGs were functionally known genes. Four genes including Pscd2 were GV-specific, 10 genes including PKD2 and CSN3 were highly expressed in GV oocytes(GV-selective), and 12 genes including Diva were highly expressed in MII oocytes (MII-selective). Ail of the genes identified in this study were first reported in the oocyte expression using ACP system and especially, we could characterize the existence of PKD-CSW signaling pathwayin the mouse oocytes. Results of the present study would provide insight for studying molecular mechanisms regulating oocyte maturation.

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Structural Safety Analysis of Openable Working Table in ACP Hot Cell for Spent Fuel Treatment (사용후핵연료 처리를 위한 ACP 실증시설내 개폐형 작업대의 구조적 안전성 평가)

  • Kwon, Kie-Chan;Ku, Jeong-Hoe;Lee, Eun-Pyo;Choung, Won-Myung;You, Gil-Sung;Lee, Won-Kyung;Cho, Il-Je;Kuk, Dong-Hak
    • Journal of Nuclear Fuel Cycle and Waste Technology(JNFCWT)
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    • v.4 no.1
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    • pp.17-24
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    • 2006
  • A demonstration facility for advanced spent fuel conditioning process (ACP) is under construction in KAERI. In this hot cell facility, all process equipments and materials are taken in and out only through the rear door. The working table in front of the process rear door is specially designed to be openable for the efficient use of the space. This paper presents the structural safety analysis of the openable working table, for the normal operational load condition and accidential drop condition of heavy object. Both cases are investigated through static and dynamic finite element analyses. The analysis results show that structural safety of the working table is sufficiently assured and the working table is not collapsed even when an object of 500 kg is dropped from the height of 50 cm.

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Heritability Estimates of Sink and Source Characters by $F_2$ and $F_5$ Correlation in Rice ($F_2$$F_5$ 상관에 의한 벼 Sink 및 Source 관련형질의 유전력 추정)

  • Ha, Woon-Goo;Kim, Ho-Yeong;Choi, Hae-Chune;Lim, Sang-Jong;Suh, Hak-Soo;Lim, Moo-Sang
    • KOREAN JOURNAL OF CROP SCIENCE
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    • v.47 no.3
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    • pp.151-156
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    • 2002
  • The heritability of sink-source characters was estimated by regression coefficient between F$_2$ and F$_{5}$ in two crosses of IR 66738-118-1-2/Gayabyeo and the Ilpumbyeo/YR 15965 Acp 33. In order to get the some basic information for breeding of high yielding rice, genetic resources of new plant type with low tiller and heavy panicle were used. Most of the sink and source characters in Ilpumbyeo/YR 15965 Acp 33 cross showed high heritability over than 0.224. But the specific leaf area and sink-source ratio displayed low heritability being 0.009 and 0.013, respectively. Heritability of all sink and source characters in IR 66738-118-1-2/Gayabyeo cross ranged from 0.115 to 0.247. Correlation coefficient between yield and yield components in both combination were in the ranged between 0.001 and 0.247. But correlations among the yield components were not significant. Correlations between the yield and sink-source characters in both combinations were also highly significant. Particularly, correlation between the grain tiling ratio and most of the sink-source characters in IR 66738-118-1-2/Gayabyeo cross were not significant. In Ilpumbyeo/YR 15965 Acp 33 cross, correlations between grain filling ratio and source characters were positively significant. but sink characters (sink capacity and sink-source ratio, etc) were negatively significant.

Preparation of Calcium Carbonate with High Purity by using Ammonium Chloride Process and Ammonium Nitrate Process (Ammonium Chloride Process 및 Ammonium Nitrate Process를 이용한 고순도 탄산칼슘의 제조)

  • Kang Mee-Sook;Soh Gowan-Soon;Shin Dong-Hwa
    • Journal of Food Hygiene and Safety
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    • v.19 no.4
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    • pp.203-208
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    • 2004
  • The shells of Anadarac tegillarca granosa, Crassostrea gigas, Crassostrea nippona, and Patinopecten yessoensis were used for preparation of calcium carbonate with high purity. Calcium content in ash of shell was the highest $64.9\%$ in Anadarac tegillarca granosa ashed for 5 hr at $900^{\circ}C$, and followed as Patinopecten yessoensis $62.5\%$, Crassostrea gigas $62.4\%$, and Crassostrea nippona $61.5\%$. Whiteness of ash was the highest 81.6-85.8 in Patinopecten yessoensis shell. Calcium contents in calcium carbonates made with shells of Anadarac tegillarca granosa by using ammonium chloride process (ACP) and ammonium nitrate process (ANP) were higher $40.03-40.04\%$ than $39.92\%$ in Anadarac tegillarca granosa ash. Calcium content was the highest $40.04\%$ in pH adjusted calcium carbonate prepared by using ANP. Whiteness of calcium carbonate prepared by using ACP and ANP was the level of 101.0-101.5. Therefore, we estimated that the calcium carbonate made by using ACP and ANP could be used potentiality as a food additive for calcium supplement.

Variations of Agronomic Traits on the Progenies of the Different Callus Origin and Subculture Period in Rice (벼에서 캘러스 유기원와 계대배양 기간에 따른 재분화 계통의 주요 농업형질 변이)

  • Yi, Gi-Hwan;Oh, Byeong-Geun;Yang, Sae-Jun;Kim, Soon-Chul;Nam, Min-Hee
    • Journal of Plant Biotechnology
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    • v.30 no.4
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    • pp.365-368
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    • 2003
  • Variations of agronomic traits were evaluated on the progenies of regenerated rice plants of different callus origin and subculture period. From eighty-eight percent to ninety percents of ovary culture-derived plants (OCP) and anther culture-derived plants (ACP) were not segregated within the lines in major agronomic characters. Compare to ACP and OCP lines, the seed culture derived lines (SCP) showed more segregation (17%) in major agronomic traits among the lines. The most frequent segregating traits were grain fertility (6.1%) in ACP, leaf color (4.2%) in OCP and grain fertility (4.9%) and leaf color (4.9%) in SCP lines. The SCP line showed more variation in culm length, panicle length and heading date than those of OCP and ACP lines. The variation of agronomic traits in SCP lines was tended to increase with prolonged subculture. Culm and panicle length were shorter than those of original cultivar in all three types of tissue culture-derived lines (OCP, ACP and SCP).