Journal of Plant Biotechnology

  • 제30권4호
  • /
  • Pages.365-368
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  • 2003
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  • 1229-2818(pISSN)
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  • 2384-1397(eISSN)
한국식물생명공학회 (The Korean Society of Plant Biotechnology)
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벼에서 캘러스 유기원와 계대배양 기간에 따른 재분화 계통의 주요 농업형질 변이

Variations of Agronomic Traits on the Progenies of the Different Callus Origin and Subculture Period in Rice

  • Yi, Gi-Hwan (National Yeongnam Agricultural Experiment Station, RDA) ;
  • Oh, Byeong-Geun (National Yeongnam Agricultural Experiment Station, RDA) ;
  • Yang, Sae-Jun (National Yeongnam Agricultural Experiment Station, RDA) ;
  • Kim, Soon-Chul (National Yeongnam Agricultural Experiment Station, RDA) ;
  • Nam, Min-Hee (National Yeongnam Agricultural Experiment Station, RDA)
  • 발행 : 2003.12.01
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초록

벼의 미수분 자방배양으로부터 분화된 후대집단 (0$_2$)의 주요 농업적 형질특성을 조사하고 이를 약배양 및 현미배양으로부터 분화된 후대집단과 상호비교하여 본 바, 주요농업형질의 계통내 고정도는 자방배양에서 87.9%,약에서는 89.8%,현미배양은 82.6%로 나타나 약배양과 마찬가지로 자방배양에서도 고정도가 높았다. 주요 형질별 분리양상을 보면 자방배양에서는 엽색이 4.2%로 가장 빈도가 높았으며, 약배양에서는 임성 (6.1%),현미배양에서는 엽색 (4.9%),임성 (4.9%)순이었다. 또한 캘러스를 1달과 2달 계대배양한 후대의 경우 간장에서 각각 8.9%와 10.0%로 분리하는 형질의 계통비율이 높게 나타났다. 간장, 수장, 출수기에 대한 변이성 정도는 자방배양에서는 약배양과 비슷한 정도의 변이를 보인 반면, 현미배양에서는 크게 나타났으며 간장과 수장의 평균은 모두 모품종인 화영벼에 비하여 줄어들었고 출수기는 다소 지연되는 경향을 보였다.

Variations of agronomic traits were evaluated on the progenies of regenerated rice plants of different callus origin and subculture period. From eighty-eight percent to ninety percents of ovary culture-derived plants (OCP) and anther culture-derived plants (ACP) were not segregated within the lines in major agronomic characters. Compare to ACP and OCP lines, the seed culture derived lines (SCP) showed more segregation (17％) in major agronomic traits among the lines. The most frequent segregating traits were grain fertility (6.1％) in ACP, leaf color (4.2％) in OCP and grain fertility (4.9％) and leaf color (4.9％) in SCP lines. The SCP line showed more variation in culm length, panicle length and heading date than those of OCP and ACP lines. The variation of agronomic traits in SCP lines was tended to increase with prolonged subculture. Culm and panicle length were shorter than those of original cultivar in all three types of tissue culture-derived lines (OCP, ACP and SCP).

키워드

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