• Journal of Microbiology and Biotechnology
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• 제8권4호
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• pp.412-415
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• 1998

The accumulation process of $Pb^{2＋}$ in an industrial strain of Saccharomyces cerevisiae proved to be temperature-dependent, and was quite similar to chemical adsorption at the initial stage of $Pb^{2＋}$accumulation. The initial $Pb^{2＋}$ accumulation rate increased from 11.4 to 46.2 mg $Pb^{2＋}$/g cell dry weight/day, in response to the increased temperature from $20^{\circ}C\;to\;50^{\circ}C$ while the maximal $Pb^{2＋}$ accumulation amount (175.8 mg $Pb^{2＋}$/g cell dry weight) was achieved at $30^{\circ}C$. The maximal $Pb^{2＋}$/ accumulation amount with temperature was independent of ion exchange with $K^＋\;and\;Mg^{2+}$.

• 한국환경농학회지
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• 제16권2호
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• pp.124-129
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• 1997

중금속오염폐수처리에 미생물 이용 가능성을 검토하기 위하여 중금속에 강한 내성을 지님과 동시에 균체내 중금속 축적능력이 우수한 중금속 내성균을 분리하여 온도, pH, 및 균체 전배양시간 등과 같은 외부 요인에 따른 균체내 중금속 축적변화를 조사한 결과는 다음과 같다. 중금속화하물 종류에 따른 균체내 중금속 축적량은 $-nitrate(NO_3)$ group을 가진 중금속화합물에서 약간 높았으나 화합물 종류에 따라서 큰 차이는 없었다. 균체 전배양시간에 따른 중금속 축적은 24시간 전배양한 균체에서 높게 나타났으나 전반적으로 전배양시간에 따라서 큰 차이는 없었다. 중금속 내성균의 균체내 중금속 축적은 온도, pH등 외부환경요인에 영향을 받았으며, 중금속 내성균의 중금속 최적축적 온도는 전반적으로 $20{\sim}37^{\circ}C$ 범위였고, 중금속 최적축적 pH는 전반적으로 $6{\sim}8$ 범위였다. 중금속을 처리한 용액중 중금속 내성균의 균체량을 증가시킬수록 균체내 축적된 총 중금속양은 증가되었으나 균체 단위 g당 중금속 축적량은 감소되었으며, 균체량의 증가에 비례해서 중금속 축적량이 증가되지는 않았다.

• 한국지리정보학회지
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• 제18권2호
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• pp.45-58
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• 2015

본 연구는 낙동강 부유쓰레기에 대한 실태 조사와 이동 경로 추적 시험을 통해 주요 집적 구간을 파악하고자 하였다. 또한, 주요 집적 구간을 대상으로 표본 조사를 실시하여 부유쓰레기의 유입량 및 성상을 파악하였다. 그 결과 낙동강 중 하류 지역으로부터 총 5곳의 집적 구간이 선정되었으며 대량의 초목류와 생활쓰레기가 집적되어 있었다. 그리고 하류 지역에 위치한 2곳의 집적 구간에서는 부유쓰레기가 해양으로 유출될 가능성이 큰 것으로 예측되었다. 주요 집적 구간에 대한 표본 조사에서는 초목류와 생활쓰레기를 포함한 $40{\ell}$이상의 쓰레기가 수거되었다. 생활쓰레기의 성상을 분류한 결과 플라스틱류가 49%를 차지하였으며 스티로폼류가 36%로 대부분을 차지하였다. 향후 본 연구는 낙동강 부유쓰레기의 해양 유입을 줄이고 효율적인 사전 수거 활동을 계획하는데 도움을 줄 것으로 기대된다.

• 한국미생물·생명공학회지
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• 제23권3호
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• pp.352-358
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• 1995

In this study, authors investigated the cadmium tolerance, the accumulation of cadmium, and the cellular distribution of accumlated cadmium in lactic acid bacteria under the anaerobic condition. Lactic acid bacteria grew fairly well in modified EG medium containing 10 ppm of cadmium but could hardly grow at 50 ppm of cadmium. Tolerance to cadmium of genus Lactobacillus was greater than that of genus Streptococcus, and Lactobacillus acidophilus showed the higest cadmium tolerance amomg the bacteria tested. The capacity of cadmium accumlation (9.304-12.428 mg/g wet cell) of lactic acid bacteria was higher than that (6.775 mg/g wet cell) of Escherichia coli. Lactobacillus casei of them took up the largest amount of cadmium. The cadmium elimination amount (28.46-29.25%) of lactic acid bacteria from modified EG medium containing cadmium were also higher than that (14.43%) of Escherichia coli. Accumulated cadmium in Lactobacillus acidophilus was distributed by 42.41% at cell wall, 28.97% at cytoplasm, and 28.62% at plasma membrane, respectively.

• Weed & Turfgrass Science
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• 제2권1호
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• pp.82-87
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• 2013

본 연구는 한국잔디의 재배기간 동안 생육 및 무기성분 축적량을 조사하여 한국잔디의 재배전략을 수립하는데 기초자료로 활용하고자 수행하였다. 한국잔디의 경우 다른 작물과 달리 시비와 잔디깎기 등과 같은 관리에 따라 생장 및 무기양분 축적에 차이가 있었다. 잔디깎기로 인해 부위별로 무기양분이 손실되는 양에 비해 축적되는 양이 적었다. 무기양분 축적 차이는 있었으나 K, Ca, Mg은 N과 비슷한 양상을 보였으며, 무기양분의 축적 순서는 N > K > P > Ca > Mg 이었다. 이와 같은 한국잔디의 무기양분 축적 및 흡수특성은 재배의 기초로서 시비량의 결정이나 토양관리 면에서 각 무기양분에 대한 요구성과 서로 밀접한 관계가 있다. 한국잔디의 생육 및 밀도율 향상에 따른 생산량을 늘리기 위해서는 생육시기별 무기양분 축적 파악을 통해 생육시기에 맞추어서 효율적으로 양분을 공급하는 것이 바람직하므로 그에 따른 기초 연구가 필요할 것으로 판단되었다.

• 한국작물학회지
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• 제46권5호
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• pp.387-394
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• 2001

Blackgram (Vigna mungo) is more salt tolerant than mungbean (Vigna radiata). This study was initiated to know whether the accumulation pattern of mineral ions in different plant parts plays a significant role in the differences in salt tolerance between the two Vigna species. Different mineral ions, viz. N, Cl, Na, K, Mg and Ca in different organs of two varieties of each of blackgram- Barimash-l (susceptible one) and Barimash-2 (tolerant one), and mungbean-Barimung-3 (tolerant one) and Barimung-4(susceptible one), were analyzed after growing with 0, 50, 75 and 100 mM NaCl solutions. The two crops showed a decreased but similar pattern of total N accumulation under saline conditions. The tolerant variety of both the crops showed a less reduction in total N than the susceptible one. Leaves showed the maximum while stem the minimum N, irrespective of levels of salinity. C $l^{[-10]}$ and N $a^{+}$ accumulation increased with the increasing salinity levels. Interestingly, similar to a halophyte, the salt tolerant blackgram exhibited conspicuously higher amount of N $a^{+}$ in the shoot than the salt-susceptible mungbean. However, the tolerant varieties showed less amount of N $a^{+}$ than the susceptible one, especially in blackgram. Seeds of both Vigna spp. accumulated the minimum amount of N $a^{+}$ than other plant parts. $K^{+}$ accumulation decreased by salinity in most of the plant parts, except seeds. Blackgram showed larger reduction in K than mungbean. The $Mg^{++}$ increased in leaves, petioles and stem by salinity while decreased in the roots, podshells and seeds in both the crops. Salinity increased $Ca^{++}$ accumulation in all plant-parts except roots of both Vigna spp. Apparently, the leaves of mungbean accumulated higher concentration of $Ca^{++}$ than blackgram. Varietal differences in the accumulation pattern of $K^{+}$, $Mg^{++}$ and $Ca^{++}$ were not clear. It was concluded that blackgram, presumably, possesses a similar salt tolerance mechanism to halophyte, and the pattern of accumulation of mineral ions in blackgram and mungbean was not fully ascribed to the differences in salinity tolerance between the two Vigna species.gna species.ies.s.ies.

• 한국환경복원기술학회지
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• 제11권5호
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• pp.1-11
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• 2008

This study was carried out to provide primary data set for cleaning of contamination site. By having hydroponic culture on Salix alba and S. caprea seedling treated with Ni solution, the result of Ni accumulation came out as followings : In a treatment of Ni to the Salix alba and S. caprea, Ni accumulation increased in its root, leaves, and stem, as Ni concentration became higher until $10.0{\mu}mol$. But in a $100.0{\mu}mol$ treatment, the seedlings died after 4 weeks. Ni accumulation in the Salix alba and S. caprea was the highest in its roots, second-highest in leaves, and the lowest in stems. In the case of $10.0{\mu}mol$ treatment of Ni solution, Ni accumulation in roots were above 500.0mg/L, and leaves were above 20.0mg/L. But it was lower than 13.0mg/L in stems. Ni accumulation in the plant increased more when nutrient solution containing Ni was weekly changed than just refilling the same amount of nutrient solution that evaporated Ni accumulation in Salix alba was higher than S. caprea when the nutrient solution had been refilled only.

• Journal of Nutrition and Health
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• 제27권8호
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• pp.828-836
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• 1994

A low level exposure experiment was conducted on growing rats to investigate the accumulation and organ distribution of protein bound cadmium compared with cadmium chloride. Male Sprague-Dawley rats were fed for 21days with one of the semisynthetic diets, which contains cadmium as either bovine liver- or kidney meal bound cadmium, cadmium chloride with uncontaminated liver meal or cadmium chloride without organ meal, in the levels of ca. 0.5, 1 and 1.5mg/kg diet, respectively. After 21days of exposure cadmium was accumulated in liver, kidney and gastrointestinal tracts depending upon cadmium levels in diet. Inspite of very low cadmium accumulation in whole blood, it tends also to increase with dietary cadmium levels. The blood cadmium concentration of animals fed organ meal containing diets was about 4-7 fold higher than that without organ meal, regardless of cadmium was intrinsically bound to protein or not. However, significant effects of organ protein on cadmium accumulation in liver, kidney and digestive tracts were not detectable, when cadmium was supplemented as cadmium chloride. On the other hands, animals fed diet containing ca. 1.5mg Cd/kg as organ bound cadmium retained more cadmium in liver, kidney and digestive tracts compared to cadmium chloride with organ meal, whereby the increase of cadmium concentration in kidney was greater then in liver. However, when the concentration of protein bound cadmium was<1mg/kg diet, organ bound cadmium was not significantly different from cadmium chloride in bioavailability and organ distribution. From this result it is suggested that the intestinal absorption of protein bound cadmium is influenced of the amount of cadmium bound in protein. When cadmium concentration in protein is relatively low, protein bound cadmium seems to be absorbed in the same way as cadmium ions are absorbed. However, when the concentration is high, at least a small amount of intact protein bound cadmium could be absorbed and accumulated selectively in kidney.

• Journal of Microbiology and Biotechnology
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• 제1권3호
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• pp.220-226
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• 1991

Large and rapid increases in benzophenanthridine alkaloid production occured in suspension cultures of Eschscholtzia californica cells treated with elicitors. Response to different biotic elicitors showed that elicitors prepared from yeast extract, Collectotrichum lindemuthianum and Verticillium dahliae induced alkaloid formation. Highest alkaloid accumulation was obtained with $60\;\mu\textrm{g}$ of yeast extract elicitor per gram of fresh cell weight. In time course performance after elicitor addition, more than 40 hours were required to obtain saturated alkaloid accumulation. Compounded silicone fluid, an ideal accumulation phase for two-phase culture of E. californica, accumulated a large amount of alkaloids produced in a specific manner. Elicitation in two-phase culture clearly increased net alkaloid production as well as their concentrations in the accumulation phase.

• 한국식품영양학회지
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• 제11권6호
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• pp.600-605
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• 1998

Present study was investigate to evaluate the aluminum absorption effect on lactic acid bacteria(Lactobacillus acidophilus ATTC 4356, Lactogacillus bulgaricus ATTC 11842, Lactobacillus casei IFO 3533, and Streptococcus thermophilus KCTC 2185 ; LAB) and Clostridium perfringens ATCC 3627 (CP) in artificial intestinal tract. Their growth rate, aluminum accumulation and cellular distribution was studied under anaerobic broth system. All of above microbes were inhibited by adding 10 to 100ppm of aluminum. The degree of aluminum in LAB (Lactobacillus acidophilus ATCC 4356, Lactobacillus bulgaricus ATCC 11842, Lactobacillus casei IFO 3533, and Streptococcus thermophilus KCTC 2185) was higher than of CP. The largest amount of aluminum was accumulated in Lactobacillus bulgaricus ATCC 11842. Aluminum accumulation in LAB was distributed in 49.1% at cell wall, 27.3% at plasma membrane, and 23.6% at cytoplasm, respectively. This study suggests that LAB might help to eliminate the ingested aluminum in intestinal tract.