• Microbiology and Biotechnology Letters
• /
• v.13 no.3
• /
• pp.297-302
• /
• 1985

The 7.1 Mdal Xbaf fragment of Bacillus pasteurii ATCC 11859 containing gene for urease was inserted into the Xbal site of bifunctional plasmid pGR71, and its urease gene was cloned and expressed in E. coil RRI. But the cloned gene was not expressed in Bacillus subtilis BR151 in consequence of deletion of inserted DNA fragment. The recombinant plasmid thus formed was named pGU66. The restriction map of the plasmid pGU66 was determined, and the size of the plasmid was estimated to be 12.6 Mdal by double digestion of restriction enzymes of the plasmid. The urease of the cloned strain was accumulated in periplasmic space and very similiar to that of donor strains in their enzymatic properties.

• Proceedings of the Korean Society for Noise and Vibration Engineering Conference
• /
• 2011.10a
• /
• pp.488-493
• /
• 2011

Authors previously suggested the design sensitivity analysis based on transmissibility function and identified the sensitivity of measured point over the small modification of system dynamics. On the other hand, the acceleration data will not reveal the strain information at the same location and authors suggested energy isoclines that successfully predict the fatigue damage on the interesting location to overcome the drawback of acceleration over fatigue society. Both of methodologies, sensitivity analysis and fatigue damage prediction, commonly use the response acceleration response as main indicator. In this paper, authors investigate the advanced method of vibration fatigue prediction using design sensitivity analysis to enhance the accuracy of predicted accumulated fatigue. Uni-axial vibration testing is performed with finite element model of a simple notched specimen and the prediction of fatigue damage at notched location is conducted for accelerations at different measurement locations that show different sensitivity contribution, either.

• Transactions of Materials Processing
• /
• v.26 no.4
• /
• pp.210-215
• /
• 2017

In this paper, utilizing the theory of ductile fracture a chevron crack in a 4-stage open cold extrusion process is predicted by the finite element methods and then compared with previous experiments. The normalized Cockcroft-Latham damage model is employed and the material is identified using a tensile test based material identification technique that gives fracture information as well as flow stress at large strain. A large difference between the predicted cracks and actual experiments is observed, specifically narrower width and greater maximum height of the crack. This reveals the limitation of this approach based on the conventional theory of ductile fracture. Based on the observations and the related criticisms, a new approach for predicting the chevron crack is proposed, suggesting that either the critical damage should not be a fixed material constant, or that the conventional fracture theory should be considered with the effects of embrittlement due to accumulated plastic deformation while the duration of crack generation and plastic deformation should be reduced.

• Biotechnology and Bioprocess Engineering:BBE
• /
• v.9 no.3
• /
• pp.196-200
• /
• 2004

The supra molecular weight poly(〔R〕-3-hydroxybutyrate) (PH B), having a molecular weight greater than 2 million Da, has recently been found to possess improved mechanical properties compared with the normal molecular weight PHB, which has a molecular weight of less than 1 million Da. However, applications for this PHB have been hampered due to the difficulty of its production. Reported here, is the development of a new metabolically engineered Escherichia coli strain and its fermentation for high level production of supra molecular weight PHB. Recombinant E. coli strains, harboring plasm ids of different copy numbers containing the Alcaligenes latus PHB biosynthesis genes, were cultured and the molecular weights of the accumulated PHB were compared. When the recombinant E. coli XL1-Blue, harboring a medium-copy-number pJC2 containing the A. latus PHB biosynthesis genes, was cultivated by fed-batch culture at pH 6.0, supra molecular weight PHB could be produced at up to 89.8 g/L with a productivity of 2.07 g PHB/L-h. The molecular weight of PHB obtained under these conditions was as high as 22 MDa, exceeding by an order of magnitude the molecular weight of PHB typically produced in Ralstonia eutropha or recombinant E. coli.

• Proceedings of the Korean Institute of Building Construction Conference
• /
• 2014.11a
• /
• pp.214-215
• /
• 2014

Recently, Russia decided to export an annual 7.5 million tons of natural gas to Korea over 30 years from 2015, as also deal with China, planed to build a pipeline connecting Siberia to Shandong Peninsula about 4000km. Risk management is required depending on the project in extreme cold weather, because it is concerned about the behavior of the seasonal changes in soil temperature and the strain of pipe according to the long-distance pipeline construction. The plan of data management shall be prepared in parallel for a sophisticated risk management, because a data is massive scale and it is generated/accumulated in real time. Therefore, this research is aimed to classify a data items in engineering stage of pipeline by previous studies for managing a generated data depending on the detail works in extreme cold weather. We expect to be provided the foundation of an efficient classification system of a generated data from the pipeline project life cycle.

• Earthquakes and Structures
• /
• v.7 no.5
• /
• pp.843-859
• /
• 2014

In the present study, the seismic performance of structural walls with boundary elements confined by conventional tie hoops and steel fiber concrete (SFC) was investigated. Cyclic lateral loading tests on four wall specimens under constant axial load were performed. The primary test parameters considered were the spacing of boundary element transverse reinforcement and the use of steel fiber concrete. Test results showed that the wall specimen with boundary elements complying with ACI 318-11 21.9.6 failed at a high drift ratio of 4.5% due to concrete crushing and re-bar buckling. For the specimens where SFC was selectively used in the plastic hinge region, the spalling and crushing of concrete were substantially alleviated. However, sliding shear failure occurred at the interface of SFC and plain concrete at a moderate drift ratio of 3.0% as tensile plastic strains of longitudinal bars were accumulated during cyclic loading. The behaviors of wall specimens were examined through nonlinear section analysis adopting the stress-strain relationships of confined concrete and SFC.

• The Korean Journal of Zoology
• /
• v.31 no.3
• /
• pp.218-224
• /
• 1988

Restriction endonucleases were used to search for intraspecific variation at 32 cleavage sites in mitochondrial DNA(mtDNA) purified from fourteen strains of Drosophila melanogaster helonging to different localities of the world. mtDNA of D. melanogaster was displayed site variation(Hpall, Haelll and Seal endonucleases) and length variation(maxirnum 550bp). Six genotypes, Ml, M2, M3, M4, M6 and M7, could be distinguished based on ihe site types witti a low average of intraspecific substitution rate (1.88%),but M5 type of Ogasawara strain in Japan was not detected in this study. A possible explanation for the low divergence was that mtDNA variation of fourteen strains in D. melanogaster could not he accumulated sufficiently owing to recent divergence of few individuals, and that sequence divergence was prevented by frequent migration in spite of the geographical isolation.

• Transactions of the Korean Society for Noise and Vibration Engineering
• /
• v.24 no.11
• /
• pp.840-845
• /
• 2014

Vibration profiles consist of two kinds of pattern, random and harmonic, at general engineering problems and the detailed vibration test mode of a target system is decided by the spectral condition that is exposed under operation. In moving mobility, random responses come generally from road source; whereas the harmonic responses are triggered from rotating machinery parts, such as combustion engine or drive shaft. Different spectral input may accumulate different damage in frequency domain since the accumulated fatigue damage dependent on the pattern of input spectrum in high cyclic loading condition. To evaluate the sensitivity of spectral damage according to different loading conditions, a linear elastic system is introduced to conduct a uniaxial vibration testing. Measured data, acceleration and strain, is analyzed using energy isocline function and then, the calculated fatigue damage is compared by different loading cases, random and harmonic.

• Geomechanics and Engineering
• /
• v.37 no.4
• /
• pp.359-370
• /
• 2024

The primary goal of this study is to evaluate the migration of fine granular materials into overlying layers under cyclic loading using a modified large-scale triaxial system as a physical model test. Samples prepared for the modified large-scale triaxial system comprised a 60 mm thick gravel layer overlying a 120 mm thick subgrade layer, which could be either tailings or railway sand. A quantitative analysis of the migration of fine granular materials was based on the mass percentage and grain size of migrated materials collected in the gravel. In addition, the cyclic characteristics, i.e., accumulated axial strain and excess pore water pressure, were evaluated. As a result, the total migration rate of the railway sand sample was found to be small. However, the total migration rate of the sample containing tailings in the subgrade layer was much higher than that of the railway sand sample. In addition, the migration analysis revealed that finer tailings particles tended to be migrated into the upper gravel layer easier than coarser tailings particles under cyclic loading. This could be involved in significant increases in excess pore water pressure at the last cycles of the physical model test.

• Nuclear Engineering and Technology
• /
• v.56 no.5
• /
• pp.1638-1653
• /
• 2024

Given the unpredictability of the occurrence of the earthquake and other potential disasters into consideration, the nuclear power plant may be confronted with beyond design-basis earthquake load in the future. The containment structure may be severely damaged under such severe earthquake loading, increasing the risk of containment concrete cracking and potential radioactive materials leaking. Moreover, initial damage caused by the earthquake may significantly alter the pressure performance of the containment under follow-up internal pressure. To compromise the dangers of beyond design-basis earthquake to the containment, an alternative of replacing the conventional concrete with fiber-reinforced concrete (FRC) to upgrade the seismic resistance capacity of the containment is attempted and thoroughly researched. In this study, the influence of various fiber types such as rigid fiber and mixed fiber is regarded to constitute fiber-reinforced PCCVs. The physical properties of traditional and fiber-reinforced PCCVs under earthquake ground motions are scientifically compared and identified by using traditional and proposed evaluation indices. The results indicate that both the traditional evaluation index (i.e. top displacement, stress, strain) and the proposed damage index are greatly reduced by the practice of fiber strengthening under earthquake ground motions.