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Statistical Optimization of Medium Components for Milk-Clotting Enzyme Production by Bacillus amyloliquefaciens D4 Using Wheat Bran-an Agro-Industry Waste

  • Zhang, Weibing;He, Xiaoling;Liu, Hongna;Guo, Huiyuan;Ren, Fazheng;Gao, Weidong;Wen, Pengcheng
    • Journal of Microbiology and Biotechnology
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    • v.23 no.8
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    • pp.1084-1091
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    • 2013
  • In this paper, two statistical methods were applied to optimize medium components to improve the production of the milk-clotting enzyme by Bacillus amyloliquefaciens D4. First, wheat bran juice, skim milk powder, and $Na_2HPO_4$ were shown to have significant effects on D4 enzyme production using the Plackett-Burman experimental design. Subsequently, an optimal medium was obtained using the Box-Behnken method, which consisted of 3.31 g/l of skim milk powder, 5.0 g/l of sucrose, 0.1 g/l of $FeSO_4{\cdot}7H_2O$, 0.1 g/l of $MgSO_4{\cdot}7H_2O$, 0.1 g/l of $MnSO_4{\cdot}2H_2O$, 0.1 g/l of $ZnSO_4{\cdot}7H_2O$, 1.52 g/l of $Na_2HPO_4$, and 172.45 g/l of wheat bran juice. With this optimal medium, the milk-clotting enzyme production was remarkably enhanced. The milk-clotting enzyme activity reached 3,326.7 SU/ml after incubation of 48 h, which was 1.76-fold higher than that of the basic medium, showing that the Plackett-Burman design and Box-Behnken response surface method are effective to optimize medium components, and B. amyloliquefaciens D4 possessed a high rennet-producing capacity in the optimal medium.

Effects of Silkworm (Bombyx mori L.) Powder on Oxygen Radicals and Their Scavenger Enzymes in Liver of SD Rats (간장조직의 활성산소 및 그 제거효소에 미치는 누에(Bombyx mori L.) 분말의 영향)

  • 최진호;김대익;박수현;김정민;조원기;이희삼;류강선
    • Journal of Life Science
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    • v.10 no.4
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    • pp.347-353
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    • 2000
  • This study was designed to investigate the effects of silkworm (Bombyx mori L.) powder (SWP) on oxygen radicals and their scavenger enzymes in liver membranes of rats. Sprague-Dawley(SD) male rats(160$\pm$10g) were fed basic diet (control group), and experimental diets(SWP-200 and SWP-400 groups) added 200 and 400 mg/kg BW/day for 6 weeks. Hydroxyl radical (.OH) levels resulted in a consistent decreases (4.0% and 7.2%, 5.0% and 14.1%, respectively) in liver mitochondria and microsomes of SWP-200 SWP-400 groups compared with control group, and O2 radical level was significantly decreased about 12% in liver cytosol of SWP-400 group compared with control group. Lipid peroxide(LPO) levels were significantly decreased (14.4% and 9.1%, respectively) in liver mitochondria and microsomes of SWP-400 group only compared with control group. Oxidized protein (OP) levels were remarkably decreased about 12.7% and 16.3% in liver microsomes only of SWP-200 and SWP-400 groups, but significant difference between liver mitochondria could not obtained. Mn-SOD activities were remarkably increased (15.8% and 25.2%, respectively) in mitochondria of SWP-200 and SWP-400 groups, but significant difference between Cu, Zn-SOD activities in these group could be not obtained. GSHPx activity was significantly increased in liver cytosol of SWP-400 group compared with control group. These results suggest that silkworm powder may play an effective role in a attenuating a oxidative stress and increasing a scavenger enzyme activity in liver membranes.

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Organic Dispersion Type Back Light EL Display Device as a New Light Source (신광원 유기분산형 백라이트 EL 디스플레이 소자)

  • 임인호;박종주;장관식;정회승;박창엽
    • Journal of the Korean Institute of Illuminating and Electrical Installation Engineers
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    • v.14 no.1
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    • pp.1-6
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    • 2000
  • In this paper, organic dispersion type back light EL(Electroluminescent) devices were manufactured using Ethyl hydroxy ethyl cellulose as organic binder, ZnS:Cu as phosphor powder and $BaTiO_3$ as dielectrics by screen printing method, which are focused on as a new light source. The properties of the fabricated organic dispersion type back light EL devices were showed $1.98[mA/\m^2]$ of current density, 0.075[W] of power consumption, 7.1[nF] of capacitance at $25[^{\circ}C]$, 100[V], 400[Hz], respectively. Also brightness of the fabricated device was revealed $20~110[cd/\m^2]$ at 50~150[V] and the change of color was shoed bluish green of x=0.1711, y=0.3676 which are color coordinate by CIE.

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Visible light assisted photocatalytic degradation of methylene blue dye using Ni doped Co-Zn nanoferrites

  • Thakur, Preeti;Chahar, Deepika;Thakur, Atul
    • Advances in nano research
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    • v.12 no.4
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    • pp.415-426
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    • 2022
  • Nickel substituted cobalt-zinc ferrite nanoparticles with composition Co0.5Zn0.5NixFe2-xO4 (x = 0.25, 0.5, 0.75, 1.0) were synthesized using a wet chemical method named citrate precursor method. Various characterizations of the prepared nanoferrites were done using X-ray powder diffractometry (XRD), Scanning electron microscopy (SEM), UV visible spectroscopy and Fourier transform spectroscopy technique (FT-IR). XRD confirmed the formation of cubic spinel structure of the samples with single phase having one characteristic peak at (311). The value of optical band gap (Eg) was found to decrease with Ni substitution and have values in the range 2.30eV to 1.69eV. A Fenton-type system was created by photocatalytic activity using source of visible light for removal of methylene blue dye. Observations revealed increase in the degradation of methylene blue dye with increasing nickel content in the samples. The degradation percentage was increased from 77.32% for x = 0.25 to 90.16% for x = 1.0 in one hour under the irradiation of visible light. Also, the degradation process was found to have pseudo first order kinetics model. Hence, it can be observed that synthesized nickel doped cobalt-zinc ferrites have good capability for water purification and its degradation efficiency enhanced with increase in nickel concentration.

A Study on the Optimum Design for LTCC Micro-Reformer: (Performance Evaluation of Various Flow Channel Structures ('LTCC를 소재로 하는 마이크로 리포머의 최적 설계에 관한 연구: (다양한 채널구조에 따른 성능변화 고찰)')

  • Chung Chan-Hwa;Oh Jeong-Hoon
    • Proceedings of the Korean Society of Precision Engineering Conference
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    • 2006.05a
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    • pp.551-552
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    • 2006
  • The miniature fuel cells have emerged as a promising power source for applications such as cellular phones, small digital devices, and autonomous sensors to embedded monitors or to micro-electro mechanical system (MEMS) devices. Several chemicals run candidate at a fuel in those systems, such as hydrogen. methanol, ethanol, acetic acid, and di-methyl ether (DME). Among them, hydrogen shows most efficient fuel performance. However, there are some difficulties in practical application for portable power sources. Therefore, more recently, there have been many efforts for development of micro-reformer to operate highly efficient micro fuel cells with liquid fuels such as methanol, ethanol, and DME In our experiments, we have integrated a micro-fuel processor system using low temperature co-fired ceramics (LTCC) materials. Our integrated micro-fuel processor system is containing embedded heaters, cavities, and 3D structures of micro- channels within LTCC layers for embedding catalysts (cf. Figs. 1 and 2). In the micro-channels of LTCC, we have loaded $CuO/ZnO/Al_2O_3$ catalysts using several different coating methods such as powder packing or spraying, dipping, and washing of catalyst slurry.

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Interfacial Defects in $SiO_2$-Glass Bond During VCR Head Fabrication (VCR 헤드 제조시 $SiO_2$박막과 유리의 계면 결함)

  • Yun, Neung-Gu;Hwang, Jae-Ung;Go, Gyeong-Hyeon;An, Jae-Hwan;Je, Hae-Jun;Hong, Guk-Seon
    • Korean Journal of Materials Research
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    • v.4 no.1
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    • pp.31-36
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    • 1994
  • The bonding behavior of $SiO_{2}$ thin film and glass during VCR head fabrication was investigated, varying the surface roughness of substrate and the sputtering parameter. Insufficient fillings of grooves In the $SiO_{2}$ film with glass was postulated to give rise to the generation of bubble in the glass. The surface roughness of $SiO_{2}$ film was found to depend on that of substrate. The lower the deposition rate, the smoother the surface of film. The bubble free glass after bonding could be obtained using substrate polished with 0.05$\mu\textrm{m}$ $Al_2O_3$ powder under the sputtering condition of 10% oxygen pressure.

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Partially Dry-Transferred Graphene Electrode with Zinc Oxide Nanopowder and Its Application on Organic Solar Cells (ZnO 나노 분말 코팅 기반 건식전사 그래핀 전극 제작 및 유기태양전지 응용)

  • Jo, Yeongsu;Woo, Chae Young;Hong, Soon Kyu;Lee, Hyung Woo
    • Journal of Powder Materials
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    • v.27 no.4
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    • pp.305-310
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    • 2020
  • In this study, partially dry transfer is investigated to solve the problem of fully dry transfer. Partially dry transfer is a method in which multiple layers of graphene are dry-transferred over a wet-transferred graphene layer. At a wavelength of 550 nm, the transmittance of the partially dry-transferred graphene is seen to be about 3% higher for each layer than that of the fully dry-transferred graphene. Furthermore, the sheet resistance of the partially dry-transferred graphene is relatively lower than that of the fully dry-transferred graphene, with the minimum sheet resistance being 179 Ω/sq. In addition, the fully dry-transferred graphene is easily damaged during the solution process, so that the performance of the organic photovoltaics (OPV) does not occur. In contrast, the best efficiency achievable for OPV using the partially dry-transferred graphene is 2.37% for 4 layers.

The Recycling of Inorganic Industrial Waste in Cement Industry (시멘트산업에서 무기질 산업 폐·부산물의 재활용)

  • Kang, S.K.;Nam, K.U.;Seo, H.N.;Kim, N.J.;Min, K.S.;Chung, H.S.;Oh, H.K.
    • Clean Technology
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    • v.6 no.1
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    • pp.61-69
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    • 2000
  • In this study, generation process and properties of inorganic industrial waste which can be used in cement industry were investigated. The scheme of recycling to use the selected waste as raw materials, mineralizer and flux, admixture and raw materials for special cement was decided and then various experiments were carried out. The experimental results were as follows ; In the use of industrial waste as raw materials, ferrous materials could be substituted by Cu-slag, Zn-slag, electric arc furnace or convertor furnace slag etc., and a siliceous material could be substituted by sand from cast-iron industry. By-products from sugar or fertilizer industry, which has $CaF_2$ as the main component, and jarosite from Zn refinery enabled clinker phases to be formed at lower temperature by $100{\sim}150^{\circ}C$. Adding Cu slag and STS sludge in proper proportion to cement improved properties of cement. Fly ash and limestone powder as admixture had the same effect on cement. As a raw material for special cement, aluminium waste sludge could be used in making ultra early strength cement, which had the compressive strength of $300kg/cm^2$ within 2hours. And two different ashes from municipal incinerator could be raw materials of the cement which was mainly composed of $C_3S$ and $C_{11}A_7{\cdot}CaCl_2$ as clinker phases.

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Mineralization of Geothite and Lepidocrocite on the Twisted-stalk and Sheathed-filaments from the Microbial Origin (미생물 기원의 나선형 및 협막구조물에 형성된 침철석과 레피도크로사이트의 결정화작용)

  • Park, Cheon-Young;Cho, Sang-Seob
    • Economic and Environmental Geology
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    • v.42 no.6
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    • pp.549-559
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    • 2009
  • The objectives of this study are to investigate the biomineralization of goethite and lepidocrocite formed on the twisted-stalk and the sheathed-filament structure that is suggest microbe origin, and heavy metal in the yellow Fe-hydroxide. On the ratio of Cl and the Cl/Br ratios that are a pollution and non-pollution of groundwaters, it is indicated that the groundwater in this areas were relatively contaminated by human activity. The composition of the yellow Fe-hydroxide consisted mainly of $Fe_2O_3$ and $SiO_2$. The content of $Fe_2O_3$ ranges from 58.57 wt.% to 75.7 3wt.%, and $SiO_2$ content ranges from 5.8 wt.% to 16.17 wt.%. Heavy metal elements such as Zn(max. 6,160 mg/kg), Pb(max. 377 mg/kg), U(max. 503 mg/kg), Cr(max. 203 mg/kg), Cu(max. 77 mg/kg), V(max. 162 mg/kg), Ni(max. 105 mg/kg) were observed to be rich in those yellow Fe-hydroxide. The lath and platy crystals and needle-shaped crystals were clearly observed on the twisted-stalks and sheathed-filaments structure. The goethite, gypsum, and lepidocrocite were identified in the yellow Fe-hydroxide by x-ray powder diffraction.

Experimental Investigation for Shear Modulus of MRE due to Magnetic Field and Volume Percent of CIP (자기장과 CIP 성분비 변화에 따른 MRE의 전단탄성계수에 대한 실험적 조사)

  • Yoon, Ji-Hyun;Fawazi, N.;Yoon, K.;Chung, K.;Oh, Jae-Eung
    • Proceedings of the Korean Society for Noise and Vibration Engineering Conference
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    • 2008.04a
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    • pp.753-753
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    • 2008
  • Magneto-rheological elastomers(MRE) are similar to magnetorheological fluids. Thus, rheological properties of MRE can be also controlled by an applied magnetic field. The MRE in this experiment is a mixture of natural rubber(NR), carbonyl iron powder(CIP), ZnO, and sulfur. Three specimens were prepared and tested by using the vibration testing instruments. The magnetic field was generated by the electromagnets. Natural frequencies of the oscillator were changed by the applied magnetic field. The properties of the MRE were increased due to magnetic field strength.

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