Journal of Microbiology and Biotechnology

The Korean Society for Microbiology and Biotechnology (한국미생물·생명공학회)
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Statistical Optimization of Medium Components for Milk-Clotting Enzyme Production by Bacillus amyloliquefaciens D4 Using Wheat Bran-an Agro-Industry Waste

  • Zhang, Weibing (College of Food Science and Technology Engineering, Gansu Agricultural University) ;
  • He, Xiaoling (Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University) ;
  • Liu, Hongna (College of Food Science and Technology Engineering, Gansu Agricultural University) ;
  • Guo, Huiyuan (Key Laboratory of Functional Dairy, College of Food Science and Nutritional Engineering, China Agricultural University) ;
  • Ren, Fazheng (College of Food Science and Technology Engineering, Gansu Agricultural University) ;
  • Gao, Weidong (College of Food Science and Technology Engineering, Gansu Agricultural University) ;
  • Wen, Pengcheng (College of Food Science and Technology Engineering, Gansu Agricultural University)
  • Received : 2012.12.20
  • Accepted : 2013.05.01
  • Published : 2013.08.28
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Abstract

In this paper, two statistical methods were applied to optimize medium components to improve the production of the milk-clotting enzyme by Bacillus amyloliquefaciens D4. First, wheat bran juice, skim milk powder, and $Na_2HPO_4$ were shown to have significant effects on D4 enzyme production using the Plackett-Burman experimental design. Subsequently, an optimal medium was obtained using the Box-Behnken method, which consisted of 3.31 g/l of skim milk powder, 5.0 g/l of sucrose, 0.1 g/l of $FeSO_4{\cdot}7H_2O$, 0.1 g/l of $MgSO_4{\cdot}7H_2O$, 0.1 g/l of $MnSO_4{\cdot}2H_2O$, 0.1 g/l of $ZnSO_4{\cdot}7H_2O$, 1.52 g/l of $Na_2HPO_4$, and 172.45 g/l of wheat bran juice. With this optimal medium, the milk-clotting enzyme production was remarkably enhanced. The milk-clotting enzyme activity reached 3,326.7 SU/ml after incubation of 48 h, which was 1.76-fold higher than that of the basic medium, showing that the Plackett-Burman design and Box-Behnken response surface method are effective to optimize medium components, and B. amyloliquefaciens D4 possessed a high rennet-producing capacity in the optimal medium.

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References

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