• 제목/요약/키워드: Zingiberis rhizoma

검색결과 103건 처리시간 0.022초

Antithrombotic Activity of Sunghyangjunggisan

  • Park, Eun-Kyung;Yoon, Hae-Kyung;Kim, Dong-Hyun
    • Natural Product Sciences
    • /
    • 제8권2호
    • /
    • pp.71-75
    • /
    • 2002
  • As apart of our continuing search for antistroke agents from the herbal medicinal resources, we examined in vitro, ex vivo and in vivo the possibility of Sunghyangjunggisan and its ingradients as a novel antithrombotic agent. In vitro ADP- and collagen-induced rat platelet aggregations were potently inhibited by Arisaematis Rhizoma, Cinnamomi Cortex and Zingiberis Rhizoma in a dose-dependent manner, but not by Sunghyangjunggisan. However, Sunghyangjunggisan significantly inhibited ex vivo rat platelet aggregation. Arisaematis Rhizoma, Atractylodis Rhizoma Alba, and Pinelliae Rhizoma also significantly inhibited ex vivo rat platelet aggregation. Sunghyangjunggisan, Alpiniae Fructus and Zingiberis Rhizoma showed significant protection from death due to pulmonary thrombosis in mice. Therefore, Sunghyangjunggisan can express the antithrombotic action, when it is orally administered.

뇌신경소교세포(腦神經小膠細胞)에서 생강 헥산 분획물의 염증매개물질 생성(生成) 억제효과(抑制效果) (Hexane Fraction of Zingiberis Rhizoma Crudus Extract Inhibits the Production of Nitric Oxide and Pro-inflammatory Cytokines in LPS-stimulated BV2 Microglial Cells)

  • 정환용;주예진;정혜미;신우진;서운교
    • 대한한의학회지
    • /
    • 제30권2호
    • /
    • pp.17-29
    • /
    • 2009
  • Objectives: The present study is focused on the inhibitory effect of the rhizome hexane fraction extract of Zingiberis Rhizoma Crudus (ginger hexan extract; GHE) on the production of inflammatory mediators such as NO, $PGE_2$, and proinflammatory cytokines in lipopolysaccharide (LPS)-stimulated BV2 cells, a mouse microglial cell line. Methods: We separated the hexane fraction from Zingiberis Rhizoma Crudus's methanol extract. The inhibitory and anti-inflammatory effect of GHE was examined on microglial activation. Results: GHE significantly inhibited the excessive production of NO, $PGE_2$, TNF-${\alpha}$, and IL-1${\beta}$ in LPS-stimulated BV2 cells. In addition, GHE attenuated the mRNA expressions and protein levels of inducible nitric oxide synthase (iNOS), cyclooxygenase-2 (COX-2), and proinflammatory cytokines. Conclusion: The anti-inflammatory properties of GHE may make it useful as a therapeutic candidate for the treatment of human neurodegenerative diseases.

  • PDF

보기, 보양, 온리약에 의해 유발된 이온전류와 흥분성 신경전달물질인 glutamate 수용체와의 관계 (Relationship of Bogi, Boyang, Onri herbs activated ion currents and excitatory neurotransmitter, glutamate, receptor)

  • 이충열;김연정;김창주
    • 동의생리학회지
    • /
    • 제14권2호통권20호
    • /
    • pp.55-66
    • /
    • 1999
  • The effects of Bogi, Boyang and Onri herbs on glutamate receptor using nystatin perforated patch clamp under voltage clamp were investigated and the following results was obtained. Ginseng radix and Astragali radix were chosen as Bogi herbs. Cervi cornu and Boshniakiae herba were used as Boyang herbs. Aconiti tuber and Zingiberis rhizoma were selected as Onri herbs. 1. $10^{-6}M$ of glycine brought about the increase of the ion currents by Astragali radix. Cervi cornu. Boshniakiae herba and Aconiti tuber to a minute extent that has no experimental meaning. 2. It was assumed that Bogi herb had no effect on glutamate receptor since the ion currents of Ginseng radix and Astragali radix that are Bogi herbs had not been inhibited to a great extent by D-AP5 and CNQX. 3. Boyang herbs were seem to have meaningful relationship with non-NMDA receptor since the ion currents by Cervi cornu and Boshniakiae that are Boyang herbs were inhibited by CNQX but not inhibited to a meaningful extent by D-AP5. 4. D-AP5 had not a significant effect on the ion currents activated by Onri herbs such as Aconiti tuber and Zingiberis rhizoma. CNQX had a significant effect on the ion currents of Aconiti tuber but no effect on the ion currents of Zingiberis rhizoma. Therefore, it seemed that Aconiti tuber had a relationship with non-NMDA receptor while Zingiberis rhizoma had no relationship with glutamate receptor. In summary, Bogi, Boyang and Onri herbs had different effects through different mechanisms on glutamate receptor. Hence it was believed that. through experiments on acetylcholine receptor new classification could be made.

  • PDF

환도(GB30) 및 족삼리(ST36) 건강약침이 신경병증성 통증 유발 흰쥐에 미치는 영향 (Effects of Zingiberis Rhizoma Pharmacopuncture Injected at GB30 and ST36 on Neuropathic Pain in Rats)

  • 황민섭
    • Korean Journal of Acupuncture
    • /
    • 제36권1호
    • /
    • pp.52-62
    • /
    • 2019
  • Objectives : The objective of this study was to investigate the effects of Zingiberis Rhizoma Pharmacopuncture(ZP) at GB30 and ST36 in neuropathic pain induced SD rats by the block of Transient Receptor Potential Vanilloid 1(TRPV1). Methods : Neuropathic pain in rats was induced by tibial and common peroneal nerve transection of right leg. The rat subjects were divided into 6 groups : normal(Nor, n=5), control(Con, n=5), neuropathic pain plus 2 mg/kg ZP injection at GB30 and ST36(ZP-A, n=5), 10 mg/kg ZP(ZP-B, n=5), 20 mg/kg ZP(ZP-C, n=5) and 0.45 mg/kg Tramadol(Tra, n=5). Three days after the surgery, injections were administered once a day for 17 days. Withdrawal response of neuropathic rats' legs were measured by stimulating the paw of Right leg with von frey filament, acetone and radient heat on day 3, 7, 11, 15, 19 after surgery. After all treatments were completed, c-Fos in the midbrain central gray and TRPV1 & TRPA1 of DRG(L5) were analyzed. Results : Groups ZP-B and ZP-C showed a meaningful decrease in the withdrawal response of mechanical allodynia, thermal hyperalgesia and cold allodynia compared to the control group(p<0.05, p<0.01, p<0.001). Groups ZP-B and ZP-C showed a meaningful decrease in the expression of c-fos and TRPV1 protein level compared to the control group(p<0.05, p<0.01, p<0.001). Conclusions : These results suggest that Zingiberis Rhizoma Pharmacopuncture at GB30 and ST36 could decrease mechanical & cold allodynia and thermal hyperalgesia by block the TRPV1 on the model of neuropathic pain.

수치에 따른 건강 중 6-Gingerol의 함량 비교 (Content of 6-Gingerol in Zingiberis Rhizoma by Roasting)

  • 서창섭;김정훈;신현규;김병수
    • 생약학회지
    • /
    • 제48권3호
    • /
    • pp.226-231
    • /
    • 2017
  • Zingiberis Rhizoma (ZR, Zingiberaceae) is one of the foods for a long time and contains various biological activities including anti-cancer, anti-inflammatory, anti-oxidant, and anti-platelet activities. The present study was to compare the amount of 6-gingerol in the 70% ethanol extracts of non-processed ZR and processed ZR by roasting using a high-performance liquid chromatography equipped with photodiode array detector. The 6-gingerol was separated on a Gemini $C_{18}$ analytical column ($5{\mu}m$, $4.6{\times}250mm$) using two mobile phase consisting of distilled water and acetonitrile. The flow rate was 1.0 mL/min and injection volume was $10{\mu}L$. In non-processed ZR sample, the amount of the 6-gingerol was 0.43% and the amount of the marker compound in processed ZR samples by roasting were 0.16-0.55%.

Apoptosis Induction Effect of Zingiberis Rhizoma Extract in Microglia BV-2 Cells

  • Seo, Jeongbin;Oh, Myung Sook;Jang, Young Pyo;Kim, Jeong Hee
    • International Journal of Oral Biology
    • /
    • 제42권1호
    • /
    • pp.9-15
    • /
    • 2017
  • Microglia have multiple functions in regulating homeostasis of the central nervous system. Microglia cells have been implicated as active contributors to neuron damage in neurodegenerative disorders. In this study, medicinal plant extracts (MPEs) were used to evaluate the cell-death induction effect in microglia BV-2 cells. Among 35 MPEs tested in this study, 4 MPEs showed less than a 30% cell survival after 24 hours of incubation. These were Foeniculi Fructus, Forsythiae Fructus, Zingiberis Rhizoma and Hedera Rhombea. The concentration showed that 50% cell death ($IC_{50}$) occurred with 33, 83, 67 Ed highlight: Please confirm wording, and $81{\mu}/ml$, respectively. For further study, we chose Zingiberis Rhizoma (ZR) which showed a reasonably low $IC_{50}$ value and an induction of cell death in a relatively narrow range. Western blot analysis showed that ZR-treated cells showed activation of caspase-3 and cleavage of PARP Ed highlight: When an acronym is first presented it needs to be spelled out in both dose- and time-dependent manners. However, the level of Bcl-2 and Bax were not changed by ZR-treatment in BV-2 cells. These results suggest that ZR-induced apoptosis in BV-2 cells occured through caspase-3 activation. The results also suggested that ZR may be useful in developing treatments for neurodegenerative diseases.

건강 추출물의 뇌세포 보호 작용 (Neuroprotective Effects of the Extract of Zingiberis Rhizoma)

  • 정길생;리빈;이동성;최현규;김윤철
    • 생약학회지
    • /
    • 제41권3호
    • /
    • pp.190-195
    • /
    • 2010
  • Glutamate-induced oxidative injury contributes to neuronal degeneration in many central nervous system (CNS) diseases, such as Parkinson's disease, Alzheimer's disease, epilepsy and ischemia. Inducible heme oxygenase (HO)-1 acts against oxidants that are thought to play a role in the pathogenesis of these diseases. NNMBS098, a composition comprising the water insoluble of the 70% EtOH extract of Zingiberis Rhizoma, showed the potent neuroprotective effects on glutamateinduced neurotoxicity by induced the expression of heme oxygenase (HO)-1 and increased HO activity in the mouse hippocampal HT22 cells. Furthermore, NNMBS098 caused the nuclear accumulation of nuclear factor E2-related factor 2 (Nrf2) in mouse hippocampal HT22 cells. In addition, we found that treatment with c-Jun N-terminal kinase (JNK) inhibitor (SP600125) reduced NNMBS098-induced HO-1 expression and NNMBS098 also increased JNK phosphorylation. Therefore, these results suggest that NNMBS098 increases cellular resistance to glutamate-induced oxidative injury in mouse hippocampal HT22 cells, presumably through JNK pathway-Nrf2-dependent HO-1 expression.

향사양위탕(香砂養胃湯)의 기원(基源), 변천과정(變遷過程) 및 구성원리(構成原理) (The Origin, Changes and Compositive Principles of Hyangsayangyi-tang)

  • 강미정;이준희;고병희;이의주
    • 사상체질의학회지
    • /
    • 제22권3호
    • /
    • pp.43-50
    • /
    • 2010
  • 1. Objectives: The purpose of this study is to understand the origin, changes and the constructive principles of Hyangsayangyitang (XiangShaYangWeiTang). 2. Methods: Hyangsayangyi-tang and other related prescriptions were analyzed in terms of pathology, based on "Donguibogam(東醫寶鑑)", "Donguisusebowon Chobongwon(東醫壽世保元 草本卷)", "Donguisusebowon Gabobon(東醫壽世保元 甲午本)", "Donguisusebowon Sinchukbon(東醫壽世保元 辛丑本)". 3. Results and Conclusions: (1) The origin of Hyangsayangyi-tang is Hyangsayukgunja-tang from "Gongshin Uigamseo(龔信 醫鑑書"). Hyangsayangyi-tang inherited some of the principles from"Euihangangmok(醫學綱目)" Jeonssiyigong-san's (全氏異功散) application. (2) "Gabobon(甲午本)"'s prescription emphasizes the effect of lossen up by using Pogostemonis Herba(藿香). "Sinchukbon(辛丑本)"'s prescription emphasizes the effect of warm up stomach by using Zingiberis Rhizoma(乾薑). (3) Ginseng Radix(人蔘), Atractlodis Rhizoma White(白朮), Glycyrrhizae Radix(甘草), Paeoniae Radix Alba(白芍藥) support Soeumin's spleen root (脾元), and Zingiberis Rhizoma(乾薑), Myristicae Semen(肉豆蔲) warm up stomach, and Pinelliae Rhizoma(半夏), Citri Pericarpium(陳皮) circulate Qi, Crataegi Fructus(山楂), Amomi Fructus(砂仁), Cyperi Rhizoma(香附子) encourage digestion to maintain descending Yin.

생약(生藥)의 항산화활성검색(抗酸化活性檢索) 연구(硏究) (Antioxidant Activity Screening on Crude Drugs)

  • 한병훈;유시용;박명환;이혜정
    • 생약학회지
    • /
    • 제10권3호
    • /
    • pp.108-111
    • /
    • 1979
  • In vivo antioxidant activities were screened over 30 kinds of crude drugs which are most frequently prescribed in oriental medicine. Of these, only Ginseng Radix, Cimicifugae Rhizoma, Zingiberis Rhizoma(steam dried), Alismatis Rhizoma, and Liriopes Tuber were shown to be positive in the activity.

  • PDF

소청룡탕(小靑龍湯)의 군신좌사(君臣佐使) 배오(配伍) 분석 (Compatibility Analysis Through the System of Chief, Deputy, Assistant, and Envoy for Socheongnyong-tang)

  • 김도회;윤미정;신순식
    • 대한한의학방제학회지
    • /
    • 제26권4호
    • /
    • pp.363-380
    • /
    • 2018
  • Objectives : We analyzed the principle of compatibility of socheongnyong-tang and expanded the range of application in clinical practice. Methods : Socheongnyong-tang was analyzed by the compatibility principle through the system of chief, deputy, assistant, and envoy, but the basic formulas of "Discussion of Cold Damage" and "Synopsis of the Golden Chamber" was used. Results : Socheongnyong-tang treats exterior wind-cold and interior water accumulation. Exterior wind-cold is treated mainly by exterior-releasing herbs constituting mahwang-tang and gyeji-tang in "Discussion of Cold Damage", and interior water accumulation is treated mainly by retained fluid-resolving herbs constituting yeonggamomigangsinha-tang, yeonggamomigagangsinbanhahaengin-tang and yeonggamomigagangsinbanhaengdaehwang-tang in "Synopsis of the Golden Chamber". Depending on the weight of exterior wind-cold and interior water accumulation, the disease condition is classified as a case where exterior wind-cold is heavier than interior water accumulation, a case where exterior wind-cold is lighter than interior water accumulation, and a case where it is equivalent. When exterior wind-cold is heavier than interior water accumulation, chief herbs are Ephedrae Herba and Cinnamomi Ramulus of exterior-releasing herbs series and deputy herbs are Zingiberis Rhizoma and Asari Radix et Rhizoma of retained fluid-resolving herbs series. When exterior wind-cold is equal to the disease condition of interior water accumulation, chief herbs are Ephedrae Herba of exterior-releasing herbs series and Zingiberis Rhizoma of retained fluid-resolving herbs series, and deputy herbs are Cinnamomi Ramulus of the exterior-releasing herbs series and Asari Radix et Rhizoma of retained fluid-resolving herbs series. When exterior wind-cold is lighter than interior water accumulation, chief herbs are Zingiberis Rhizoma and Asari Radix et Rhizoma of retained fluid-resolving herbs series and deputy herbs are Ephedrae Herba and Cinnamomi Ramulus of exterior-releasing herbs series. In any case, assistant herbs are Pinelliae Rhizoma, Paeoniae Radix alba, and Schisandrae Fructus, and envoy herb is Glycyrrhizae Radix preparata. Conclusions : In conclusion, socheongnyong-tang must differently formulate the system of chief, deputy, assistant, and envoy according to the grade of exterior wind-cold and interior water accumulation. These results suggest that socheongnyong-tang can be applied flexibly when applied in clinical practice to enhance the therapeutic effect.