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http://dx.doi.org/10.14406/acu.2019.033

Effects of Zingiberis Rhizoma Pharmacopuncture Injected at GB30 and ST36 on Neuropathic Pain in Rats  

Hwang, Min Sub (Department of Acupuncture & Moxibustion, College of Korean Medicine, Dongguk University)
Publication Information
Korean Journal of Acupuncture / v.36, no.1, 2019 , pp. 52-62 More about this Journal
Abstract
Objectives : The objective of this study was to investigate the effects of Zingiberis Rhizoma Pharmacopuncture(ZP) at GB30 and ST36 in neuropathic pain induced SD rats by the block of Transient Receptor Potential Vanilloid 1(TRPV1). Methods : Neuropathic pain in rats was induced by tibial and common peroneal nerve transection of right leg. The rat subjects were divided into 6 groups : normal(Nor, n=5), control(Con, n=5), neuropathic pain plus 2 mg/kg ZP injection at GB30 and ST36(ZP-A, n=5), 10 mg/kg ZP(ZP-B, n=5), 20 mg/kg ZP(ZP-C, n=5) and 0.45 mg/kg Tramadol(Tra, n=5). Three days after the surgery, injections were administered once a day for 17 days. Withdrawal response of neuropathic rats' legs were measured by stimulating the paw of Right leg with von frey filament, acetone and radient heat on day 3, 7, 11, 15, 19 after surgery. After all treatments were completed, c-Fos in the midbrain central gray and TRPV1 & TRPA1 of DRG(L5) were analyzed. Results : Groups ZP-B and ZP-C showed a meaningful decrease in the withdrawal response of mechanical allodynia, thermal hyperalgesia and cold allodynia compared to the control group(p<0.05, p<0.01, p<0.001). Groups ZP-B and ZP-C showed a meaningful decrease in the expression of c-fos and TRPV1 protein level compared to the control group(p<0.05, p<0.01, p<0.001). Conclusions : These results suggest that Zingiberis Rhizoma Pharmacopuncture at GB30 and ST36 could decrease mechanical & cold allodynia and thermal hyperalgesia by block the TRPV1 on the model of neuropathic pain.
Keywords
neuropathic pain; Zingiberis Rhizoma; pharmacopuncture; TRPV1;
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1 Nam SC, Kang H, Shim BS, Kim SH, Choi SH, Ahn KS. Angiogenic inhibitory effect of Zingiberis Rhizoma. J Korean Orient Oncology. 2006 ; 11(1) : 55-63.
2 Sharma, J.N., Srivastava, K.C., Gan, E.K. Suppressive effects of eugenol and ginger oil on arthritic rats. Pharmacology. 1994 ; 49 : 314-8. doi: 10.1159 /000139248.   DOI
3 Nickel FT, Seifert F, Lanz S, Maihofner C. Mechanisms of neuropathic pain. Eur Neuropsychopharmacology. 2012 ; 22 : 81-91.   DOI
4 Butera JA. Current and emerging targets to treat neuropathic pain. J Med Chem. 2007 ; 50(11) : 2543-6. doi: 10.1002/chin.200731182.   DOI
5 Yun DM. Neuropathic pain. The Korean Journal of Pain. 1995 ; 8(2) : 219-24.
6 E. Palazzo, L. Luongo, V. de Novellis, L. Berrino, F. Rossi, S. Maione. Moving towards supraspinal TRPV1 receptors for chronic pain relief. Molecular Pain. 2010 ; 6 : 66. doi: 10.1186/1744-8069-6-66.   DOI
7 K. M. Walker, L. Urban, S. J. Medhurst et al. The VR1 antagonist capsazepine reverses mechanical hyperalgesia in models of inflammatory and neuropathic pain. Journal of Pharmacology and Experimental Therapeutics. 2003 ; 304(1) : 56-62. doi: 10.1124/jpet.102.042010.   DOI
8 Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D. The capsaicin receptor: a heat-activated ion channel in the pain pathway. Nature. 1997 ; 389 : 816-824. doi: 10.2142/biophys.39.159.   DOI
9 Story GM, Peier AM, Reeve AJ et al. ANKTM1, a TRP-like channel expressed in nociceptive neurons, is activated by cold temperatures. Cell. 2003 ; 112 : 819-29. doi: 10.1016/s0092-8674(03)00158-2.   DOI
10 Bennett GJ, Xie YK. A peripheral mononeuropathy in rats that produces disorders of pain sensation like those seen in man. Pain. 1988 ; 33 : 87-107. doi: 10.1016/0304-3959(88)90209-6.   DOI
11 Seltzer Z, Dubner R, Shir Y. A novel behavioral model of neuropathic pain disorders produced in rats by partial sciatic nerve injury. Pain. 1990 ; 43 : 205-18. doi: 10.1016/0304-3959(90)91074-s.   DOI
12 Kim SH, Chung JM. An experimental model for peripheral neuropathy produced by segmental spinal nerve ligation in the rat. Pain. 1992 ; 50 : 355-63. doi: 10.1016/0304-3959(92)90041-9.   DOI
13 Decosterd I, Woolf CJ. Spared nerve injury: an animal model of persistent peripheral neuropathic pain. Pain. 2000 ; 87 : 149-58. doi: 10.1016/s0304-3959(00)00276-1.   DOI
14 Dowdall T, Robinson I, Meert TF. Comparison of five different rat models of peripheral nerve injury. Pharmacol Biochem Behav. 2005 ; 80(1) : 93-108. doi: 10.1016/j.pbb.2004.10.016.   DOI
15 Jongen JL, Hans G, Benzon HT, Huygen F, Hartrick CT. Neuropathic pain and pharmacological treatment. Pain Pract. 2014 ; 14(3) : 283-95.   DOI
16 Barber J. Examining the use of tramadol hydrochloride as an antidepressant. Exp Clin Psychopharmacol. 2011 ; 19(2) : 123-30. doi: 10.1037/a0022721.   DOI
17 Greenberg ME, Greene LA, Ziff EB. Nerve growth factor and epidermal growth factor induce rapid transient change on pro-to-oncogene transcription in PC12 cells. J Biol Chem. 1985 ; 206(26) : 14101-10.   DOI
18 Hunt SP, Pini A, Evan G. Induction of c-fos-like protrein in spinal cord neurons following sensory stimulation. Nature. 1987 ; 328(6131) : 632-4. doi: 10.1038/328632a0.   DOI
19 Munglani R, Hunt SP. Molecular biology of pain. Br J Anaesth. 1995 ; 75(2) : 186-92.   DOI
20 Luigi Rodella, Rita Rezzani, Cristina Agostini and Rossela Bianchi. Expression of NADP-diaphorase and colocalization with Fos in the brain neurons of the rat following viseral noxious stimulation. Brain Research. 1999 ; 834 : 173-7.   DOI
21 Suekawa, M., Ishige, A., Sudo, K., Aburada, M. and Hosoya, E. Pharmacological studies on ginger. I. Pharmacological actions of pungent constitutents, (6)-gingerol and (6)-shogaol. J Pharmacobiodyn. 1984 ; 7 : 836-48. doi: 10.1248/bpb1978.7.836.   DOI
22 Sharma, J.N., Srivastava, K.C., Gan, E.K., Suppressive effects of eugenol and ginger oil on arthritic rats. Pharmacology. 1994 ; 49 : 314-8. doi: 10.1159/000139248.   DOI
23 Young Joo Sohn, Yong Suk Kim, Young Bae Park, Ja Seung Ku, Ran Won, Nak Won Sohn, Hyuk Sang Jung. Alterations of Cerebral Metabolic Activation Folling Electro-Acupuncture Stimulation on ST36 and LR3 Acu-Points in Rats. The Journal of Korean Acupuncture & Moxibustion Society. 2002 ; 19(1) : 159-174.
24 Jensen TS, Baron R, Haanpaa M et al. A new definition of neuropathic pain. Pain. 2011 ; 152 : 2204-5. doi: 10.1016/j.pain.2011.06.017.   DOI
25 Maag R, Baron R. Neuropathic pain: translational research and impact for patient care. Curr Pain Headache Rep. 2006 ; 10(3) : 191-8. doi: 10.1007/s11916-006-0045-8.   DOI
26 Minn YK, Kim SM. Diagnosis and Treament of Neuropathic Pain. J Korean Med Assoc. 2008 ; 51(12) : 1139-48.   DOI
27 Kim SS, Ko SG, Cho KH et al. East-West Medical Study on Bi syndrome-Focusing on Causes and Symptoms. Korean J. Orient. Int. Med 1994 ; 15(1) : 116-27.
28 Kim moo-jin, yoon jong-hwa, kim kyung-ho, lee seung-deok, kim kap-sung. Acupuncture therapy literaturre study on the knee Rheumatoid Arthritis. The journal of Korean Acupuncture & Moxibustion Society. 2005 ; 22(1) : 191-201.
29 Korean Medicine Univ. Acupuncture & Moxibustion and Acupoint Department. The Acupuncture and Moxibustion 1. Seoul: Jipmoondang. 382-4, 643-5.
30 Kwon YB, Lee JD, Lee HJ, Han HJ, Mar WC, Kang SK et al. Bee Venom injection into an acupuncture point reduces arthritis associated edema and nociceptive responses. Pain. 2001 ; 90(3) : 271-80. doi: 10.1016/s0304- 3959(00)00412-7.   DOI
31 Je BS, Lim LC, Oh MS. The Literatural Study on Acupuncture and Moxibustion Therapy of Arthalgia Syndrome. Research Institute of Korean Medicine Thesis Collection. 2004 ; 13(1) : 61-9.
32 Korean Phamcopuncture Institute science committee. Pharmacopuncturology. Seoul: Elsevier Korea. 2008: 3, 6-8.
33 Caterina MJ, Schumacher MA, Tominaga M, Rosen TA, Levine JD, Julius D. The capsaicin receptor: a heatactivated ion channel in the pain pathway. Nature. 1997 ; 389 : 816-24.   DOI
34 Vellani V, Kinsey AM, Prandini M et al. Protease activated receptors 1 and 4 sensitize TRPV1 in nociceptive neurones. Mol Pain. 2010 ; 6 : 61. doi: 10.1186/1744-8069-6-61.   DOI
35 Jeonguk hanuigwadahak bonchohak gongdong gyojaepyeonchanwiwonhoe. Herbology. Seoul: Younglimsa publishing company. 2016 : 375-7.