• 한국균학회소식:학술대회논문집
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• 2016.05a
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• pp.27-27
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• 2016

The ascomycete fungus Fusarium graminearum is the most common pathogen of Fusarium head blight (FHB), a devastating disease for major cereal crops worldwide. FHB causes significant crop losses by reducing grain yield and quality as well as contaminating cereals with trichothecenes and zearalenone (ZEA) that pose a serious threat to animal health and food safety. ZEA is a causative agent of hyperestrogenic syndrome in mammals and can result in reproductive disorders in farm animals. In F. graminearum, the ZEA biosynthetic cluster is composed of four genes, PKS4, PKS13, ZEB1, and ZEB2, which encode a reducing polyketide synthase, a nonreducing polyketide synthase, an isoamyl alcohol oxidase, and a transcription factor, respectively. Although it is known that ZEB2 primarily acts as a regulator of ZEA biosynthetic cluster genes, the mechanism underlying this regulation remains undetermined. In this study, two isoforms (ZEB2L and ZEB2S) from the ZEB2 gene in F. graminearum were characterized. It was revealed that ZEB2L contains a basic leucine zipper (bZIP) DNA-binding domain at the N-terminus, whereas ZEB2S is an N-terminally truncated form of ZEB2L that lacks the bZIP domain. Interestingly, ZEA triggered the induction of both ZEB2L and ZEB2S transcription. In ZEA producing condition, the expression of ZEB2S transcripts via alternative promoter usage was directly or indirectly initiated by ZEA. Physical interaction between ZEB2L and ZEB2L as well as between ZEB2L and ZEB2S was observed in the nucleus. The ZEB2S-ZEB2S interaction was detected in both the cytosol and the nucleus. ZEB2L-ZEB2L oligomers activated ZEA biosynthetic cluster genes, including ZEB2L. ZEB2S inhibited ZEB2L transcription by forming ZEB2L-ZEB2S heterodimers, which reduced the DNA-binding activity of ZEB2L. This study provides insight into the autoregulation of ZEB2 expression by alternative promoter usage and a feedback loop during ZEA production.

• Journal of Microbiology and Biotechnology
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• v.26 no.5
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• pp.967-974
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• 2016

Zearalenone (ZEA) is an estrogenic mycotoxin that is produced by several Fusarium species, including Fusarium graminearum. One of the ZEA biosynthetic genes, ZEB2, encodes two isoforms of Zeb2 by alternative transcription, forming an activator (Zeb2L-Zeb2L homooligomer) and an inhibitor (Zeb2L-Zeb2S heterodimer) that directly regulate the ZEA biosynthetic genes in F. graminearum. Cyclic AMP-dependent protein kinase A (PKA) signaling regulates secondary metabolic processes in several filamentous fungi. In this study, we investigated the effects of the PKA signaling pathway on ZEA biosynthesis. Through functional analyses of PKA catalytic and regulatory subunits (CPKs and PKR), we found that the PKA pathway negatively regulates ZEA production. Genetic and biochemical evidence further demonstrated that the PKA pathway specifically represses ZEB2L transcription and also takes part in posttranscriptional regulation of ZEB2L during ZEA production. Our findings reveal the intriguing mechanism that the PKA pathway regulates secondary metabolite production by reprograming alternative transcription.

• Journal of Digital Convergence
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• v.20 no.2
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• pp.277-287
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• 2022

ZEB is a building that increases the energy independence of the building itself, and new and renewable elements that can produce energy are essential, and BIPV is the most notable technology. In ZEB's design, BIPV should be planned early in the design, but BIPV plans are insufficient in the early stages. Therefore, this study derived elements for theoretical consideration of BIM and ZEB and analysis of ZEB independence rate based on BIM, a convergence design technology, and analyzed BIPV energy production and building energy consumption. Finally, by calculating the energy independence rate and reviewing the rating criteria in the project model, a basic research method for calculating the energy independence rate of ZEB at the beginning of the design was presented. Through this, it is expected that design productivity can be improved by supporting the decision of ZEB subjects.

• Journal of Korean Institute of Architectural Sustainable Environment and Building Systems
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• v.12 no.6
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• pp.557-566
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• 2018

The ZEB certification system has come into effect since 2018 according to the ZEB road map. From 2020, the public buildings with the total floor area smaller than $3000m^2$ are the target of the certification, and it will be extended to the buildings with up to $5000m^2$ floor area in 2025. However, current mandatory regulations for public office buildings seem already to meet the ZEB certification system planned for 2025. In this work, two buildings belong to $3,000{\sim}5,000m^2$ in total floor area were selected to analyze the possibility of meeting the ZEB certification only by following current obligation regulations. Results showed that the test buildings satisfied the minimum building energy efficiency and energy self-sufficiency rates for the ZEB certification when the mandatory insulation and installation ratio of renewable energy are applied. This can be useful for revising the road map or extending the target buildings of the ZEB certification.

• Journal of the Korean Solar Energy Society
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• v.39 no.3
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• pp.1-7
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• 2019

As part of the government's energy policy, Zero Energy Building certification was launched on January of 2017. However, the three passive-housing rental housing projects are the only ZEB-certified detached housing since the certification's launch. The reason is that, in order for a detached housing to earn ZEB certification, it has to secure self-reliance in energy, and a photovoltaic system is the only viable renewable energy system. Therefore, conducting an analysis to optimize the photovoltaic system in an early design stage is strongly recommended. This study aimed to propose an optimal photovoltaic system design for a detached housing after analyzing through the ECO2 energy simulation of 44 cases, varying in a module type and efficiency, inclination and azimuth. As a result, 15 cases out of 44 cases were analyzed to satisfy ZEB evaluation criteria, and it is thought that these data could contribute greatly to the expansion of ZEB certification dissemination.

• Journal of the Korean Society for Geothermal and Hydrothermal Energy
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• v.19 no.1
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• pp.1-13
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• 2023

Many government in the world have conducted building energy performance certification program to reduce building energy consumption. In this study, a reference building and its HVAC system was modeled, and the energy load and consumption were estimated by the ECO2 program. The software is a simple building energy simulation program based on monthly calculated method. The building energy efficiency rating the the reference building was 1+ under baseline condition. The simulation results showed that the insulation performance slightly affected building energy load and consumption, but light density had a significant effect on them. The application of geothermal heat pumps gave improvement of building energy efficiency rating but it could not make it possible to get zero energy building(ZEB) certification. The ZEB 5 certification could be achieved by using photovoltaics, however getting better grade was difficult. The simulation results showed that the ZEB 4 certification, one grade higher than ZEB 5, could be attained by using more than one renewable energy source such as geothermal and solar energy in this study.

• Journal of the Korean Solar Energy Society
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• v.30 no.2
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• pp.39-45
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• 2010

Zero Emission Building is abuilding which emits virtually '0(zero)' carbon dioxide. Although simple in concept, ZEB requires totally different approach from conventional building in terms of design, engineering, construction and operation. There are few research on ZEB design process as ZEB design requires understanding and knowledge regarding energy and technology. The study aims to propose a design process of Zero Emission Building for architects. The study examined the concept of Zero Emission Building through intensive literature search. The examples of Zero Emission Buildings were investigated, and strategies and technologies applied to the buildings were analyzed. Various conventional design processes were identified and analyzed to examine the applicability to ZEB design, Finally, a new design process which effectively accommodate the requirement of Zero Emission Building was proposed.

• Journal of Genetic Medicine
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• v.16 no.2
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• pp.71-75
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• 2019

Periventricular nodular heterotopia (PNH) is a malformation of cortical development in which normal neurons inappropriately cluster in periventricular areas. Patients with Mowat-Wilson syndrome (MWS) typically present with facial gestalt, complex neurologic problems (e.g., severe developmental delay with marked speech impairment and epilepsy), and multiple anomalies (e.g., Hirschsprung disease, urogenital anomalies, congenital heart defects, eye anomalies, and agenesis of the corpus callosum [CC]). MWS is mostly caused by haploinsufficiency of the gene encoding zinc-finger E-box-binding homeobox 2 (ZEB2) due to premature stops or large deletions. We present a case report of a 9-year-old girl with PNH, drug-responsive epilepsy, severe intellectual disability, and facial dysmorphisms only in whom we performed whole-exome sequencing and found a de novo heterozygous missense mutation (c.3134A>C; p.His1045Pro) of ZEB2 (NM_014795.3; NP_055610.1). This mild case of MWS caused by a rare novel missense mutation of ZEB2 represents the first report of MWS with isolated PNH.

• Molecules and Cells
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• v.37 no.5
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• pp.383-388
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• 2014

Renal cell carcinoma (RCC) is associated with a high frequency of metastasis and only few therapies substantially prolong survival. Honokiol, isolated from Magnolia spp. bark, has been shown to exhibit pleiotropic anticancer effects in many cancer types. However, whether honokiol could suppress RCC metastasis has not been fully elucidated. In the present study, we found that honokiol suppressed renal cancer cells' metastasis via dual-blocking epithelial-mesenchymal transition (EMT) and cancer stem cell (CSC) properties. In addition, honokiol inhibited tumor growth in vivo. It was found that honokiol could upregulate miR-141, which targeted ZEB2 and modulated ZEB2 expression. Honokiol reversed EMT and suppressed CSC properties partly through the miR-141/ZEB2 axis. Our study suggested that honokiol may be a suitable therapeutic strategy for RCC treatment.

• The Journal of Sustainable Design and Educational Environment Research
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• v.18 no.4
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• pp.58-65
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• 2019

Under the regulation of rational energy use in public institutions, it has been mandatory for educational facilities to meet the first grade or higher since 2014. Also the regulation has forced educational facilities as public institutions to use renewable energy since September 2008. Educational facilities are required to be qualified as zero-energy buildings from 2020 under the revision of the Green Building Act. In this study, we identified the current status of the building energy efficiency rating system and the renewable energy system installation for 316 educational facilities that were accredited as "building energy efficiency rating system" from February 2015 to March 2019. Also we analyzed the energy self-sufficiency rate based on energy requirements and renewable energy output. Of the 316 facilities, 12 had 1++ and 293 had 1++ for the "Building Energy Efficiency Rating System". Among the 12 facilities which had 1+++, 11 recorded ZEB level 5 or higher, and 28 out of the 293 facilities(11%) which got received 1++ had ZEB level 5. Thus, it is impossible to implement the ZEB certification system for educational facilities under the present conditions. Expanding the ratio of 1+++ and investing in renewable energy systems should be preceded.