• Biomedical Science Letters
• /
• v.28 no.1
• /
• pp.9-24
• /
• 2022

The aim of this study was to identify the expression of zinc finger E-box binding homeobox 1 (ZEB1), its prognostic significance, and correlation between ZEB1 and infiltrating immune cells in lung cancer. Correlation between ZEB1 and telomerase was also analyzed in different types of cancers. RNA sequencing analysis and survival rates of patients were confirmed by Gene Expression Profiling Interactive Analysis (GEPIA). The Kaplan-Meier plotter and PrognoScan databases were used to analyze the prognostic value of ZEB1 in various cancers. The Tumor IMmune Estimation Resource (TIMER) was used to determine the correlation between ZEB1 and infiltrating immune cells. Lower ZEB1 expression was lower in lung cancer and was related to poor prognosis in lung adenocarcinoma (LUAD). ZEB1 expression exhibited a significantly positive correlation with infiltration levels of immune cells in LUAD and lung squamous cell carcinoma. Furthermore, we found that the ZEB1 expression correlated with subunits of telomerase. Our findings suggest ZEB1 as a potential biomarker to be used for prognostic significance and tumor immunology in lung cancer. The correlation between the expression of ZEB1 and telomere-related gene will help in understand the cancer-promoting mechanisms.

• 한국균학회소식:학술대회논문집
• /
• 2016.05a
• /
• pp.27-27
• /
• 2016

The ascomycete fungus Fusarium graminearum is the most common pathogen of Fusarium head blight (FHB), a devastating disease for major cereal crops worldwide. FHB causes significant crop losses by reducing grain yield and quality as well as contaminating cereals with trichothecenes and zearalenone (ZEA) that pose a serious threat to animal health and food safety. ZEA is a causative agent of hyperestrogenic syndrome in mammals and can result in reproductive disorders in farm animals. In F. graminearum, the ZEA biosynthetic cluster is composed of four genes, PKS4, PKS13, ZEB1, and ZEB2, which encode a reducing polyketide synthase, a nonreducing polyketide synthase, an isoamyl alcohol oxidase, and a transcription factor, respectively. Although it is known that ZEB2 primarily acts as a regulator of ZEA biosynthetic cluster genes, the mechanism underlying this regulation remains undetermined. In this study, two isoforms (ZEB2L and ZEB2S) from the ZEB2 gene in F. graminearum were characterized. It was revealed that ZEB2L contains a basic leucine zipper (bZIP) DNA-binding domain at the N-terminus, whereas ZEB2S is an N-terminally truncated form of ZEB2L that lacks the bZIP domain. Interestingly, ZEA triggered the induction of both ZEB2L and ZEB2S transcription. In ZEA producing condition, the expression of ZEB2S transcripts via alternative promoter usage was directly or indirectly initiated by ZEA. Physical interaction between ZEB2L and ZEB2L as well as between ZEB2L and ZEB2S was observed in the nucleus. The ZEB2S-ZEB2S interaction was detected in both the cytosol and the nucleus. ZEB2L-ZEB2L oligomers activated ZEA biosynthetic cluster genes, including ZEB2L. ZEB2S inhibited ZEB2L transcription by forming ZEB2L-ZEB2S heterodimers, which reduced the DNA-binding activity of ZEB2L. This study provides insight into the autoregulation of ZEB2 expression by alternative promoter usage and a feedback loop during ZEA production.

• Journal of the Korean Society for Geothermal and Hydrothermal Energy
• /
• v.19 no.1
• /
• pp.1-13
• /
• 2023

Many government in the world have conducted building energy performance certification program to reduce building energy consumption. In this study, a reference building and its HVAC system was modeled, and the energy load and consumption were estimated by the ECO2 program. The software is a simple building energy simulation program based on monthly calculated method. The building energy efficiency rating the the reference building was 1+ under baseline condition. The simulation results showed that the insulation performance slightly affected building energy load and consumption, but light density had a significant effect on them. The application of geothermal heat pumps gave improvement of building energy efficiency rating but it could not make it possible to get zero energy building(ZEB) certification. The ZEB 5 certification could be achieved by using photovoltaics, however getting better grade was difficult. The simulation results showed that the ZEB 4 certification, one grade higher than ZEB 5, could be attained by using more than one renewable energy source such as geothermal and solar energy in this study.

• Journal of the Korean Solar Energy Society
• /
• v.39 no.3
• /
• pp.1-7
• /
• 2019

As part of the government's energy policy, Zero Energy Building certification was launched on January of 2017. However, the three passive-housing rental housing projects are the only ZEB-certified detached housing since the certification's launch. The reason is that, in order for a detached housing to earn ZEB certification, it has to secure self-reliance in energy, and a photovoltaic system is the only viable renewable energy system. Therefore, conducting an analysis to optimize the photovoltaic system in an early design stage is strongly recommended. This study aimed to propose an optimal photovoltaic system design for a detached housing after analyzing through the ECO2 energy simulation of 44 cases, varying in a module type and efficiency, inclination and azimuth. As a result, 15 cases out of 44 cases were analyzed to satisfy ZEB evaluation criteria, and it is thought that these data could contribute greatly to the expansion of ZEB certification dissemination.

• The Journal of Sustainable Design and Educational Environment Research
• /
• v.18 no.4
• /
• pp.58-65
• /
• 2019

Under the regulation of rational energy use in public institutions, it has been mandatory for educational facilities to meet the first grade or higher since 2014. Also the regulation has forced educational facilities as public institutions to use renewable energy since September 2008. Educational facilities are required to be qualified as zero-energy buildings from 2020 under the revision of the Green Building Act. In this study, we identified the current status of the building energy efficiency rating system and the renewable energy system installation for 316 educational facilities that were accredited as "building energy efficiency rating system" from February 2015 to March 2019. Also we analyzed the energy self-sufficiency rate based on energy requirements and renewable energy output. Of the 316 facilities, 12 had 1++ and 293 had 1++ for the "Building Energy Efficiency Rating System". Among the 12 facilities which had 1+++, 11 recorded ZEB level 5 or higher, and 28 out of the 293 facilities(11%) which got received 1++ had ZEB level 5. Thus, it is impossible to implement the ZEB certification system for educational facilities under the present conditions. Expanding the ratio of 1+++ and investing in renewable energy systems should be preceded.

• Journal of Genetic Medicine
• /
• v.16 no.2
• /
• pp.71-75
• /
• 2019

Periventricular nodular heterotopia (PNH) is a malformation of cortical development in which normal neurons inappropriately cluster in periventricular areas. Patients with Mowat-Wilson syndrome (MWS) typically present with facial gestalt, complex neurologic problems (e.g., severe developmental delay with marked speech impairment and epilepsy), and multiple anomalies (e.g., Hirschsprung disease, urogenital anomalies, congenital heart defects, eye anomalies, and agenesis of the corpus callosum [CC]). MWS is mostly caused by haploinsufficiency of the gene encoding zinc-finger E-box-binding homeobox 2 (ZEB2) due to premature stops or large deletions. We present a case report of a 9-year-old girl with PNH, drug-responsive epilepsy, severe intellectual disability, and facial dysmorphisms only in whom we performed whole-exome sequencing and found a de novo heterozygous missense mutation (c.3134A>C; p.His1045Pro) of ZEB2 (NM_014795.3; NP_055610.1). This mild case of MWS caused by a rare novel missense mutation of ZEB2 represents the first report of MWS with isolated PNH.

• Journal of Gastric Cancer
• /
• v.20 no.2
• /
• pp.212-224
• /
• 2020

Purpose: miR-205 is a tumor suppressor and plays an important role in tumor invasiveness. However, the role of miR-205 in human gastric cancer (GC) epithelial-mesenchymal transition (EMT) remains unclear. The aim of this study was to investigate the molecular mechanism of miR-205 in the regulation of EMT in GC invasion. Materials and Methods: Quantitative polymerase chain reaction (qPCR) was used to detect the expression of miR-205 in GC. Further, the correlation between the pathological parameters and prognosis of GC was statistically analyzed. A transwell model was used to evaluate the effect of miR-205-3p on the invasion and migration of GC cells. qPCR, western blotting, and luciferase assay were performed to analyze the relationship and target effects between miR-205-3p and the expression of zinc finger electron box binding homologous box 1 (ZEB1) and 2 (ZEB2). Results: We found that the levels of miR-205-3p were significantly lower (P<0.05) in GC tissues than in matched normal tissues. Additionally, the expression of miR-205-3p was related to the tumor invasion depth, lymph node metastasis, lymph node invasion, and tumor, node, metastasis stage. Patients with lower miR-205-3p expression levels in the tumors had a poorer prognosis. The in vitro assays indicated that miR-205-3p could affect the invasion ability and EMT of GC cells by targeting the expression of both ZEB1 and ZEB2. Conclusions: miR-205-3p promotes GC progression and affects the prognosis of patients by targeting both ZEB1 and ZEB2 to directly influence EMT.

• The Journal of Sustainable Design and Educational Environment Research
• /
• v.20 no.1
• /
• pp.19-28
• /
• 2021

The Ministry of Land, Infrastructure, and Transport (MOLIT) is implementing a zero-energy building (ZEB) certification to save energy for the building section and to accelerate the achievement of national greenhouse gases reduction goals in accordance with a new climate regime. In 2014, the MOLIT announced a plan for early activation of the ZEB, and in January 2016, the "Green Buildings Construction Support Act" was revised and established. In addition, the plan was established to gradually spread zero-energy buildings from the public sector in 2020 to the private sector by 2025. Therefore, this study analyzed the self-sufficiency rate of each energy factor according to the implementation of the zero energy building certification of school facilities that belong to the public sector and are included in the mandatory zero energy buildings from 2020.

• Land and Housing Review
• /
• v.11 no.3
• /
• pp.69-74
• /
• 2020

Since the design Standard for Energy Conservation in Building was implemented in 2008 for the first time, building elements such as window and door should satisfy the minimum criteria to apply for a building. Though its regulation does not cover the whole building yet, recent demand to reduce energy consumption in building sector grows rapidly year by year and also draws a lot of interest to ensure the whole building level. For example, a Zero energy building, one of low-energy buildings, requires a customized solution to resolve the air leakage issue to meet the standards in achieving the high level of air tightness. In this study, six non-residential buildings were tested by fan pressurization method to observe the air tightness of whole building to suggest the construction guideline for air tightness of low-energy building. Five out of six tested buildings showed 0.27 to 1.16 h^-1 of number of air changes except one community center. These buildings were carefully constructed not only for building planning but also for parts where there was a concern of air leakage, thereby securing high levels of air-tightness. The construction skills were developed as a checklist to manage and supervise the construction site. It is our suggestion to use this checklist at construction sites for ZEB with the high level of air-tightness.

• Asian Pacific Journal of Cancer Prevention
• /
• v.16 no.6
• /
• pp.2473-2481
• /
• 2015

Colon cancer is one of the leading causes of cancer-associated death worldwide. The prognosis for advanced colorectal cancers remains dismal, mainly due to the propensity for metastatic progression. Accordingly, there is a need for effective anti-metastasis therapeutic agents. Since a great body of research has indicated anticancer effects for curcumin, we investigated the effects of dendrosomal curcumin (DNC) on cellular migration and adhesion of human SW480 cells and possible molecular mechanisms involved. Different methods were applied in this study including MTT, Scratch and adhesion assays as well as real-time PCR and transwell chamber assays. Based on the results obtained, DNC inhibits metastasis by decreasing Hef 1, Zeb 1 and Claudin 1 mRNA levels and can reduce SW480 cell proliferation with $IC_{50}$values of 15.9, 11.6 and $7.64{\mu}M$ at 24, 48 and 72h post-treatment. Thus it might be considered as a safe formulation for therapeutic purpose in colorectal cancer cases.