• Mass Spectrometry Letters
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• v.7 no.1
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• pp.16-20
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• 2016

Characterization of intact protein structures in the gas phase using electrospray ionization combined with ion mobility mass spectrometry has become an important tool of research. However, the biophysical properties that govern the structures of protein ions in the gas phase remain to be understood. Here, we investigated the impact of host-guest complexation of ubiquitin (Ubq) with macrocyclic host molecules, cucurbit[n]urils (CB[n]s, n = 6, 7), on its structure in the gas phase. We found that CB[n] complexation induces the formation of compact Ubq ions. Both CB[6] and CB[7] exhibited similar effects despite differences in their binding properties in solution. In addition, CB[n] attachment prevented Ubq from unfolding by collisional activation. Based on the experimental results, we suggest that CB[n]s prevent unfolding of Ubq during transfer to the gas phase to promote the formation of compact protein ions. Furthermore, interaction with positively charged residues per se is suggested to be the most important factor for the host-guest complexation effect.

• 한국신재생에너지학회:학술대회논문집
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• 2005.11a
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• pp.112-121
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• 2005

본 연구는 2003년부터 2005년까지 2년간 에너지관리공단의 선행연구과제로서 진행되었으며 20 Watt 급 소형 연료전지에 수소를 공급할 수 있는 소형의 마이크로 채널 메탄올 개질장치를 개발하는 것을 목적으로 하였다. 개질장치는 개질기 본체, 여기에 반응열을 공급 해주는 촉매 연소기 그리고 연료를 증발시켜 주는 연료증발기의 세부분으로 구성되며 각 반응기의 개발 및 통합을 수행하였다. 반응기는 반응면적을 증가시키기 위하여 폭 $200{\sim}5000{\mu}m$, 필이 $200{\sim}5000{\mu}m$ 규모의 마이크로 채널 유로를 금속 박판을 화학 에칭하여 구현하였으며 이를 수십장 적층하여 전체 반응기를 제작하였다. 마이크로 채널표면에 내부 촉매 지지체를 먼저 코팅한 후 촉매를 코팅하는 방법을 사용하여 담지체 코팅으로 기하학적 표면적 대비 표면적이 10 배 이상 향상되는 우수한 결과를 얻을 수 있었고 촉매의 내구성이 월등히 향상 되었다. 저온 활성 촉매를 사용하여 $350^{\circ}C$ 이하에서 메탄을 전환율 90% 이상을 구현하였다. 실제 운전 후 측정 결과 개질 반응기의 부분별 온도차가 $20^{\circ}C$ 이내로 설계의 우수성을 확인하였다. 촉매 연소기를 이용한 개질 반응열 공급장치를 개발하여 20Watt 급 수소 생산을 위한 개질 반응기에 반응열을 공급하도록 하였다. 이와 함께 촉매 연소기를 이용한 연료 증발열 공급 장치 개발하여 개질기 공급 연료의 90% 이상이 기화되도록 하였다.

• Korean Parent-Child Health Journal
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• v.7 no.2
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• pp.160-167
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• 2004

Purpose: The purpose of this study was to examine the effect of a preparation method using hospital play on anxiety and role behaviors of children undergoing tonsillectomy. Method: Twenty-eight children aged 5 to 7 years who were admitted for tonsillectomy were assigned to either an experimental group or a control group purposefully. The preparation method included provision of information using hospital play. The data were analyzed using Mann-Whitney test and Chi-square test. Result: There was no Significant difference in anxiety before surgery between the experimental and control groups. Also, there was no Significant difference in role behaviors on the 1st and the 2nd day of surgery. Conclusion: Even though there was no statistical significance, the result showed that the preparation method had some positive effect on children and parents. It is needed to develop systematic preparation methods for children undergoing surgery to enhance their coping.

• Review of KIISC
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• v.20 no.6
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• pp.7-16
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• 2010

인터넷 및 통신 기술의 발전은 사용자로 하여금 금융, 방송, 게임 등의 온라인 서비스 제공에 대한 시간 및 공간적 제한을 없애 주였지만, 다른 한편으로는 해커 등의 악의적 사용자로 인한 피해 가능성도 높이고 있다. 이를 해결하기 위한 다양한 보안 기법 가운데 하나가 OTP를 이용한 사용자 인증 방법이다. OTP는 재사용하지 않는 패스워드로서 기존 패스워드 인증 방식이 갖는 취약점을 해결할 수 있는 방식이다. 하지만 OTP 생성 단말의 도난이나 서버 해킹으로 인한 패스워드 추측공격 또는 Stolen verifier 공격 등에 취약할 수 있다. 본 논문에서는 위와 같은 문제점을 해결하기 위해서 두 가지 인증 정보 즉, 시간 기반 OTP 생성방식인 TOTP 및 패스워드를 이용하는 새로운 Two-Factor 인증 프로토콜인 POTP(Password embedded OTP)를 제안한다. 제안한 방식은 재전송 공격에 안전하며, 공격자가 OTP 생성용 디바이스를 획득하더라도 패스워드를 유추할 수 없고 서버의 인증 정보 데이터베이스를 획득하더라도 정상적인 사용자로 위장할 수 함께, 서버에서 인증 정보 보관시 연산 속도가 빠른 해쉬 함수를 이용할 수 있어 보다 효율적이다.

• Journal of Microbiology and Biotechnology
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• v.11 no.6
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• pp.1099-1101
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• 2001

In an attempt to search for serotonin N-acetyltransferase (arylalkylamine N-acetyltransferasem, AA-NAT) inhibitors from microbial metabolites, we fecund the culture broth of Penicillium sp. 80722 which showed a strong inhibitory activity against AA-NNT. The active principle has been identified as citrinin hydrate through bioassay-guided fractionation of cultural broth, and structure elucidation derived by spectroscopic analyses. Citrinin hydrate inhibits AA-NAT with an $IC_50$ value of $173{\mu}M$ in a dose-dependent manner. Although citrinin hydrate was previously isolated as human rhinovirus 3C-protease inhibitor, this was recognized as the first AA-NAT inhibitor isolated from natural sources.

• Journal of Powder Materials
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• v.15 no.4
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• pp.314-319
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• 2008

Nano-sized tungsten disulfide ($WS_2$) powders were synthesized by chemical vapor condensation (CVC) process using tungsten carbonyl ($W(CO)_6$) as precursor and vaporized pure sulfur. Prior to the synthesis of tungsten disulfide nanoparticles, the pure tungsten nanoparticles were produced by same route to define the optimum synthesis parameters, which were then successfully applied to synthesize tungsten disulfide. The influence of experimental parameters on the phase and chemical composition as well as mean size of the particles for the produced pure tungsten and tungsten disulfide nanoparticles, were investigated.

• Journal of Korea Multimedia Society
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• v.10 no.6
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• pp.707-715
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• 2007

The need for content-based retrieval from visual media, such as image and video data, is ever increasing rapidly in many applications, such as electronic art museums, internet shopping malls, internet search engines, and medical information systems. In our previous research, we proposed an architecture, called the HERMES, which is a Web Service-enabled visual media retrieval framework. In this paper, we propose the Web Service workflows that are employed in the HERMES. We describe how we designed the workflows for service registration and query processing in the framework. We especially explain how metadata and ontology can be utilized to realize more intelligent content-based retrieval on visual media data.

• Proceedings of the Korean Society of Propulsion Engineers Conference
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• 2011.11a
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• pp.919-921
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• 2011

Variable nozzles are used to enhance the effectiveness of aircraft engines at various altitudes. Unsynchronized movements of variable nozzle flaps affect the direction of thrust in case the variable nozzle consists of many flaps. A synchronization test system was developed to verify the synchronization characteristics of variable nozzle mechanism including flaps. The test system has a capability to simulate combustion pressure in variable nozzle space. The test system was used to qualify the synchronization characteristics of a variable nozzle flaps affected by magnitude and uniformity of simulated combustion pressure, and time delay of each nozzle actuators.

• Journal of the Korea Academia-Industrial cooperation Society
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• v.10 no.5
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• pp.955-961
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• 2009

Present, a portable battery have problem that the volume increases according to capacity Increase. Direct Methanol Fuel Cell is alternative by solution plan of this problem. In this paper, the characteristics of DMFC are analyzed by change in concentration and discharge of fuel in natural convection and room temperature condition. According to the analysis result, polarization by delay of diffusion velocity of hydrogen ion appeared in methanol of low concentration. And if have a lot of supplies of methanol, generation power declines by electric cell cooling effect.

• Proceedings of the PSK Conference
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• 2002.10a
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• pp.191-192
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• 2002

Since capsaicin was found as an excellent vanilloid receptor agonist, considerable efforts toward the development of a novel analgesic have been continued. However, the small therapeutic window between these effects and the excitatory side effects, such as hypothermia, bronchoconstriction, increased GI mobility, and hypertension, precluded the development of capsaicin as a systemic agent. (omitted)