• Korean Journal of Environmental Agriculture
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• v.33 no.3
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• pp.149-154
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• 2014

BACKGROUND: To evaluate the application level of expeller cake fertilizer(ECF), we have investigated soil chemical properties, leaf mineral contents and yield of tah tasai Chinese cabbage in a plastic film greenhouse. METHODS AND RESULTS: Five levels of fertilizer were applied as 0%(ECF 0), 50%(ECF 50), 75%(ECF 75), 100% (ECF 100) and 150%(ECF 150) by base 1,848 kg/ha of ECF. In 2012, tah tasai Chinese cabbage was planted on April 28 in a silt loam soil and harvested on July 12. Commercial yields were measured 10 times from May 10 to July 12. Electrical conductivity (2.24~3.09 dS/m), available $P_2O_5$(484~581 mg/kg) and exchangeable cations($K^+$, $Ca^{2+}$ and $Mg^{2+}$) were tended to increase by the application of ECF. However, the range of those was not significant. The contents of T-N, K, Ca and P of tah tasai Chinese cabbage leaves were 62.2~66.5 g/kg, 44.3~48.7 g/kg, 5.1~5.9 g/kg and 5.6~6.2 g/kg, respectively. The nitrogen utilization rate of tah tasai Chinese cabbage was 39.4~51.6%, and it was decreased with increased application amount of ECF. The yield of tah tasai Chinese cabbage was 9,806 to 12,730 kg/ha on the basis of application amount of ECF and it was not increased in spite of increased ECF. CONCLUSION: The optimum dose of application of ECF for cultivation of tah tasai Chinese cabbage was ranged from 924 kg/ha(as ECF 50) to 1,386 kg/ha(as ECF 75). Environment-friendly and economical amount of applied fertilizer is more important than yield for cultivation of tah tasai Chinese cabbage.

• Korean Journal of Food Science and Technology
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• v.26 no.3
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• pp.231-238
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• 1994

The brined Chinese cabbages and kimchi seasoning mixture were separately stored and used for preparation of kimchi and the alternative method of conventional kimchi-manufacturing process was investigated. Chinese cabbages brined with 3% and 7% salt contents were stored at $0^{\circ}C$ and $10^{\circ}C$, and kimchi seasoning mixture without salt was stored at $4^{\circ}C$ and $10^{\circ}C$. The changes in pH and total acid of the cabbages were significantly slowly for the brined cabbage of 7% salt and storage at $0^{\circ}C$. Microbial growth including lactic acid bacteria were also increased slowly at $0^{\circ}C$. But, pH and total acid content of the kimchi seasoning mixture were not changed significantly at both temperatures during storage. Sensory evaluation on the kimchi samples showed that the modified method of kimchi preparation with separately stored seasoning mixture and brined cabbages is useful industrially or at home.

• Korean Journal of Soil Science and Fertilizer
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• v.36 no.6
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• pp.417-422
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• 2003

The objective of this study was to assess the regime of heat shock protein and leaf temperature caused by high temperature stress in chinese cabbage grown in alpine region. In monsoon period, high temperature and heavy rain have caused a stress condition for the cultured higher plants. Chinese cabbages were grown in different altitude, i.e. 600 m and 1,100 m. It was demonstrated that heat shock protein (Hsp 90) in alpine chinese cabbage leaf was actively expressed by high temperature and surplus nitrogen application. As a results of thermo-graphically observed leaf temperatures, chinese cabbage grown in high altitude region were ranged from 20.5 to $24.3^{\circ}C$ while in low altitude from 24.0 to $31.5^{\circ}C$. Furthermore, analysis of assimilated nutrients indicated that total nitrogen content was higher in plant grown under high temperature than under low temperature.

• Journal of Food Hygiene and Safety
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• v.33 no.6
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• pp.447-452
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• 2018

Water is an important component in the production of fresh produce. It is mainly used for irrigation and application of pesticides and fertilizers. Several outbreaks cases related to fresh produce have been reported and water has been identified as the most likely source. On the other hand, wildlife has been identified as a possible source of the waterborne pathogens. The purpose of this study was to investigate the effect of wildlife access on irrigation water used in the cultivation of Chinese cabbage in Goesan. The frequency of wild animals access to upstream water source and the contamination level of bacteria such as Escherichia coli and Enterococci of irrigation water used in Chinese cabbage farm was examined. A total of 37 wildlife including the wild bear and water deer were observed in upstream of water source during the cultivation of Chinese cabbage. The result indicated the presence of hygienic indicator bacteria from the upstream where there is no human access. The contamination range of coliforms, E. coli, and Enterococcus spp. Detected in the irrigation water were 2.13~4.32 log MPN / 100 mL, 0.26~2.03 log MPN / 100 mL, and 1.43~3.49 log MPN / 100 mL, respectively. Due to low water temperatures, the contamination levels of coliform bacteria and E. coli in the irrigation water during harvesting time was lower compared to those recorded during transplanting of Chinese cabbage. However, no significant difference was detected in the number of Enterococci during the cultivation of Chinese cabbage. The results indicated the need to manage the microbial risk in irrigation water to enhance safety in cultivation of Chinese cabbage.

• Korean Journal of Food Science and Technology
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• v.26 no.3
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• pp.239-245
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• 1994

Fermentation characteristics and microbial changes during storage of brined Chinese cabbage were investigated. Ten Chinese cabbage cultivars harvested in fall were brined and stored at $0^{\circ}C$ and $10^{\circ}C$. The decrease in pH and increase in total acidity during storage were different in their tendency among the varieties. Microbial growth including lactic acid bacteria was more active at $10^{\circ}C$ and most active in `Noranmat'. The reduction of their reducing sugar contents during storage resulted in slow fermentation of kimchi prepared with those cabbage stored. From the results of sensory evaluation on kimchi prepared with each brined Chinese cabbage cultivar in storage, `Keunyureum' among the cultivats stored at $0^{\circ}C$ received the highest points on preference. But, at $10^{\circ}C$, `Sinkiwon' received the highest points and 'Noktab' received the lowest points.

• Journal of Plant Biotechnology
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• v.35 no.3
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• pp.235-243
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• 2008

Here we describe a procedure for Chinese cabbage protoplast culture and effects of various treatments. Chinese cabbage protoplasts were isolated from different parts of young seedlings as using an enzyme mixture, of which yield was maximized in seven hours around after digestion. The highest rate of initial cell division followed by micro-callus formation was obtained in the medium with 1.0 mg/L 2,4-D, 0.5 mg/L NAA, and 1.0 mg/L BA when the cotyledon-derived protoplasts were cultured. Initiation of cell division and micro-callus proliferation significantly depended upon Chinese cabbage genotype under a same culture circumstances. The micro-calli developed from cotyledon tissue of Norang-Bom cultivar successfully grew toward callus colonies on the solidified medium with 0.2 mg/L zeatin and 0.1 mM spermidine. The callus colonies generated de novo shoots at the maximum frequency of 4.3% on the medium with 5.0 mg/L BA and 1.0 mg/L NM. Our results might be helpful for further studies to enhance the regeneration efficiency in Chinese cabbage protoplast culture.

• Journal of Plant Biotechnology
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• v.35 no.2
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• pp.101-108
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• 2008

The Agrobacterium-mediated transformation has been successfully used method to introduce foreign genes into some monocotyledonous as well as a large number of dicotyledonous plants genome, We developed transgenic Chinese cabbage plants with insect-resistance gene, modified CryIAc, by Agrobacterium-transformation and confirmed transgene copy number by Southern blot analysis. We confirmed that twenty-nine out of 46 transgenic Chinese cabbage plants have single copy of CryIAc. To obtain the sequences information on the transferred DNA (T-DNA) integration into plant genome, we analyzed left border (LB) flanking sequences by genome walking (GW) PCR method. Out of 46 transgenic Chinese cabbage plants examined, 37 carried the vector backbone sequences. This result indicates that the transfer of the vector backbone from the binary vectors resulted mainly from inefficient termination of LB site. Analysis of T-DNA LB flanking region of 9 transgenic Chinese cabbage plants without vector backbone revealed that all LB ends were not conserved and nucleotides up to 36bp from the LB cleavage site were deleted.

• Korean Journal of Soil Science and Fertilizer
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• v.26 no.1
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• pp.25-30
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• 1993

It is common that in the vegetable cultivation areas, the farmers tend to continue the heavy fertilization to maximize the crop yields in short term. This results in the accumulation of fertilizer elements in the soil and increase in the electrical conductivity of soil. Disregarding these, farmers continue to apply heavy doses of fertilizers. A field experiment was conducted to find out on such a soil whether fertilizer application taking the quantity of nutrients accumulated in the soil into account would save the fertilizer without losing the yield of crop, using Chinese cabbage and spinach as test crops. The findings of the experiment is summarized as following. 1. The yield of Chinese cabbage was not affected by reduction of $19kg\;P_2O_5/10a$ and $7kg\;K_2O/10a$ as compared to farmers doses, and in case of spinach the yield rather was increased under the reduction of $22kg\;P_2O_5/10a$ and $22kg\;K_2O/10a$. 2. The reduction of fertilizers according to plant nutrient status of soils did not affect the inorganic nutrient contents of the crops significantly, in both Chinese cabbage and spinach. 3. A trend was observed that the reduction in the P and K fertilizers application would shorten the storage period of Chinese cabbage. 4. The reduction in P and K fertilizers application resulted in the reduction in available P, exchangeable K, EC and $NO_3-N$ in the soil after the harvest of the crops.

• Korean Journal of Agricultural Science
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• v.41 no.2
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• pp.125-133
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• 2014

The variation of glucosinolates (GSLs) in Chinese cabbage ('Sinhongssam') (Brassica rapa L. spp. pekinensis) cultivated under lights to control plant growth conditions was evaluated at different development stages. Under experimental conditions in plant factory system, plant growth conditions including light, temperature, and nutrients were designed to enhance GSLs. The variation of glucosinolates (GSLs) in Chinese cabbage ('Sinhongssam') (Brassica rapa L. spp. pekinensis) cultivated under lights to control plant growth conditions was evaluated at different development stages. Under experimental conditions in plant factory system, plant growth conditions including light, temperature, and nutrients were designed to enhance GSLs. The contents of GSLs were quantified in Chinese cabbage according to different light sources (Red+White, RW; Red+Blue+White, RBW, Fluorescence lamp, FL) at development stages (28, 42, and 56 days after sowing, DAS) using HPLC. Nine GSLs including five aliphatic (progoitrin, sinigrin, glucoalyssin, gluconapin, and glucobrassicanapin) three indolyl (glucobrassicin, 4-methoxyglucobrassicin, and neoglucobrassicin), and one aromatic (gluconasturtiin) GSLs were identified based on peak retention time in previous results of our laboratory. GSL contents were higher in RBW (36.55) and lower in FL ($15.24{\mu}mol/g/\;DW$). Results revealed that GSL contents were higher under controlled photoperiods (20/4 h) ($58.35{\mu}mol/g\;DW$) and controlled light intensity ($160{\mu}mol/m^2/s$) ($34.02{\mu}mol/g\;DW$), respectively. Lower amount of progoitrin and comparatively higher amount of glucobrassicin and gluconasturtiin was noted in Chinese cabbage cultivated under FL light (2.38, 9.82, and 2.10) at 42 DAS, photoperiod 20/4 h (3.16, 2.52, and 1.30) at 28 DAS, and light intensity at $130{\mu}mol/m^2/s$ (2.28, 2.24, and $1.51{\mu}mol/g\;DW$) at 42 DAS. Therefore FL light, photoperiod (20/4 h), and light intensity ($130{\mu}mol/m^2/s$) were considered as most suitable for the enhancement of GSLs in Chinese cabbage.

• Korean Journal of Agricultural and Forest Meteorology
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• v.20 no.2
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• pp.228-232
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• 2018

Process-based crop models have been used to assess the impact of climate change on crop production. These models are implemented in procedural or object oriented computer programming languages including FORTRAN, C++, Delphi, Java, which have a stiff learning curve. The requirement for a high level of computer programming is one of barriers for efforts to develop and improve crop models based on biophysical process. In this study, we attempted to develop a Chinese cabbage model using Microsoft Excel with Visual Basic for Application (VBA), which would be easy enough for most agricultural scientists to develop a simple model for crop growth simulation. Results from Soil-Plant-Atmosphere-Research (SPAR) experiments under six temperature conditions were used to determine parameters of the Chinese cabbage model. During a plant growing season in SPAR chambers, numbers of leaves, leaf areas, growth rate of plants were measured six times. Leaf photosynthesis was also measured using LI-6400 Potable Photosynthesis System. Farquhar, von Caemmerer, and Berry (FvCB) model was used to simulate a leaf-level photosynthesis process. A sun/shade model was used to scale up to canopy-level photosynthesis. An Excel add-in, which is a small VBA program to assist crop modeling, was used to implement a Chinese cabbage model under the environment of Excel organizing all of equations into a single set of crop model. The model was able to simulate hourly changes in photosynthesis, growth rate, and other physiological variables using meteorological input data. Estimates and measurements of dry weight obtained from six SPAR chambers were linearly related ($R^2=0.985$). This result indicated that the Excel/VBA can be widely used for many crop scientists to develop crop models.