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http://dx.doi.org/10.5010/JPB.2008.35.3.235

Effects of a variety of treatments affecting Chinese cabbage protoplast culture, and plant regeneration from protoplast-derived callus  

Han, Jeung-Sul (National Horticultural Research Institute, Rural Development Administration)
Yoon, Moo-Kyeong (National Horticultural Research Institute, Rural Development Administration)
Jeong, Mi-Hye (National Institute of Agricultural Science and Technology, Rural Development Administration)
Publication Information
Journal of Plant Biotechnology / v.35, no.3, 2008 , pp. 235-243 More about this Journal
Abstract
Here we describe a procedure for Chinese cabbage protoplast culture and effects of various treatments. Chinese cabbage protoplasts were isolated from different parts of young seedlings as using an enzyme mixture, of which yield was maximized in seven hours around after digestion. The highest rate of initial cell division followed by micro-callus formation was obtained in the medium with 1.0 mg/L 2,4-D, 0.5 mg/L NAA, and 1.0 mg/L BA when the cotyledon-derived protoplasts were cultured. Initiation of cell division and micro-callus proliferation significantly depended upon Chinese cabbage genotype under a same culture circumstances. The micro-calli developed from cotyledon tissue of Norang-Bom cultivar successfully grew toward callus colonies on the solidified medium with 0.2 mg/L zeatin and 0.1 mM spermidine. The callus colonies generated de novo shoots at the maximum frequency of 4.3% on the medium with 5.0 mg/L BA and 1.0 mg/L NM. Our results might be helpful for further studies to enhance the regeneration efficiency in Chinese cabbage protoplast culture.
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