Browse > Article
http://dx.doi.org/10.5010/JPB.2008.35.2.101

Analysis of junction between T-DNA and plant genome in insect resistance GM Chinese cabbage  

Lim, Sun-Hyung (Biosafety Division, National Institute of Agricultural Biotechnology, RDA)
Park, Seung-Hye (Biosafety Division, National Institute of Agricultural Biotechnology, RDA)
Kim, Jung-Hwan (Biosafety Division, National Institute of Agricultural Biotechnology, RDA)
Kim, Na-Young (Biosafety Division, National Institute of Agricultural Biotechnology, RDA)
Won, So-Youn (Biosafety Division, National Institute of Agricultural Biotechnology, RDA)
Lee, Si-Myung (Biosafety Division, National Institute of Agricultural Biotechnology, RDA)
Shin, Kong-Sik (Biosafety Division, National Institute of Agricultural Biotechnology, RDA)
Woo, Hee-Jong (Biosafety Division, National Institute of Agricultural Biotechnology, RDA)
Kim, Dong-Hern (Biosafety Division, National Institute of Agricultural Biotechnology, RDA)
Cho, Hyun-Suk (Biosafety Division, National Institute of Agricultural Biotechnology, RDA)
Publication Information
Journal of Plant Biotechnology / v.35, no.2, 2008 , pp. 101-108 More about this Journal
Abstract
The Agrobacterium-mediated transformation has been successfully used method to introduce foreign genes into some monocotyledonous as well as a large number of dicotyledonous plants genome, We developed transgenic Chinese cabbage plants with insect-resistance gene, modified CryIAc, by Agrobacterium-transformation and confirmed transgene copy number by Southern blot analysis. We confirmed that twenty-nine out of 46 transgenic Chinese cabbage plants have single copy of CryIAc. To obtain the sequences information on the transferred DNA (T-DNA) integration into plant genome, we analyzed left border (LB) flanking sequences by genome walking (GW) PCR method. Out of 46 transgenic Chinese cabbage plants examined, 37 carried the vector backbone sequences. This result indicates that the transfer of the vector backbone from the binary vectors resulted mainly from inefficient termination of LB site. Analysis of T-DNA LB flanking region of 9 transgenic Chinese cabbage plants without vector backbone revealed that all LB ends were not conserved and nucleotides up to 36bp from the LB cleavage site were deleted.
Keywords
Citations & Related Records
Times Cited By KSCI : 1  (Citation Analysis)
연도 인용수 순위
1 Abdal-Aziz SA, Pliego-Alfaro F, Quesada MA, Mercado JA (2006) Evidence of Frequent Integration of Non-T-DNA Vector Backbone Sequences in Transgenic Strawberry. Plant. J Biosci Bioeng 101: 508-510   DOI   ScienceOn
2 Alonso JM, Stepanova AN, Leisse TJ, Kim CJ, Chen H, Shinn P, Stevenson DK, Zimmerman J, Barajas P, Cheuk R, Gadrinab C, Heller C, Jeske A, Koesema E, Meyers CC, Parker H, Prednis L, Ansari Y, Choy N, Deen H, Geralt M, Hazari N, Hom E, Karnes M, Mulholland C, Ndubaku R, Schmidt I, Guzman P, Aguilar-Henonin L, Schmid M, Weigel D, Carter DE, Marchand T, Risseeuw E, Brogden D, Zeko A, Crosby WL, Berry CC, Ecker JR (2003) Genome-wide insertional mutagenesis of Arabidopsis thaliana. Science 301: 653-657   DOI   ScienceOn
3 Gamble J, Gunson A (2002) The New Zealand public's attitudes regarding genetically modified food: May and October 2001. HortReserch Client Report No. 2003/35
4 De Buck S, De Wilde C, Van Montagu M, Depicker A (2000) T-DNA vector backbone sequences are frequently integrated into the genome of transgenic plants obtained by Agrobacterium-mediated transformation. Mol Breed 6: 459-468   DOI   ScienceOn
5 Kim JS (2002) Composite of a linkage map of Brassica rapa (ssp. pekinenssis) using EST clones and comparative genome study to Arabidopsis thaliana. PhD thesis, Kyunghee University, Suwon, Republic of Korea
6 Huang S, Gilbertson LA, Adams TH, Malloy KP, Reisenbigler EK, Birr DH, Snyder MW, Zhang Q, Luethy MH (2004) Generation of marker-free transgenic maize by regular two-border Agrobacterium transformation vectors. Transgenic Res 13: 451-461   DOI
7 Kim JH, Lee S (2007) Analysis of Junctions between T-DNA and Plant genome in transgenic Arabidopsis thaliana. J of plant biology 50: 455-460   과학기술학회마을   DOI   ScienceOn
8 Lusk JL, Sullivan P (2002) Consumer acceptance of genetically modified foods. Food Technol 56: 32-37
9 Murashige T, Skoog F (1962) A revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15: 473-497   DOI
10 Small B (2004) Public perceptions about genetically engineered forage crops and resultant animal products. In: new directions for a diverse planet, proceedings of the 4th international crop science congress, Brisbane, Australia, 26 Sep-1 Oct 2004. In: T. Fisher, N. Tuner, J. Angus, L. McIntyre, M. Robertson, A. Borrell and D. Lioyd, eds. CD Rom. The Regional Institute Ltd, Gosford, Australi
11 Stahl R, Horvath H, van Fleet J, Voetz M, von Wettstein D, Wolf N (2002) T-DNA integration into the barley genome from single and double cassette vectors. Proc Natl Acad Sci USA 99: 2146-2151
12 Sha Y, Li S, Pei Z, Luo L, Tian Y, He C (2004) Generation and flanking sequence analysis of a rice T-DNA tagged population. Theor Appl Genet 108: 306-314   DOI
13 Sheng J, Citovsky V (1996) Agrobacterium-plant cell DNA transport: have virulence proteins, will travel. Plant Cell 8:1699-1710   DOI   ScienceOn
14 Sallaud C, Meynard D, van Boxtel J, Gay C, Bes M, Brizard JP, Larmande P, Ortega D, Raynal M, Portefaix M, Ouwerkerk PB, Rueb S, Delseny M, Guiderdoni E (2003) Highly efficient production and characterization of T-DNA plants for rice (Oryza sativa L.) functional genomics. Theor Appl Genet 106: 1396-1408   DOI
15 Cho HS, Cao J, Ren JP, Earle ED (2001) Control of lepidopteran insect pests in transgenic Chinese cabbage transformed with synthetic Bacillus thuringiensis cry1C gene. Plant Cell Rep 20: 1-7   DOI
16 Kononov ME, Bassuner B, Gelvin SB (1997) Integration of T-DNA vector 'backbone' sequences into the tobacco genome: evidence for multiple complex patterns of integration. Plant J 11: 945-957   DOI   ScienceOn
17 Hanson B, Engler D, Moy Y, Newman B, Ralston E, Gutter-son N (1999) A simple method to enrich an Agrobacterium-transformed population for plants containing only T-DNA sequences. Plant J 19: 727-734   DOI   ScienceOn
18 Hoekema A, Hirsh PR, Hooykass PJJJ, Schilperoort RA (1983) A binary plant vector strategy based on separation of vir- and T-region of the Agrobacterium tumefaciens Ti-plasmid. Nature 303: 179-180   DOI
19 Kim SR, Lee J, Jun SH, Park S, Kang HG, Kwon S, Ahn GH (2003) Transgene structures in T-DNA-inserted rice plants. Plant Mol Biol 52: 761-773   DOI   ScienceOn
20 Kuraya Y, Ohta S, Fukuda M, Hiei Y, Murai N, Hamada K, Ueki J, Imaseki H, Komari T (2004) Suppression of transfer of non-T-DNA 'vector backbone' sequences by multiple left border repeats in vectors for transformation of higher plants mediated by Agrobacterium tumefaciens. Mol Breed 14: 309-320   DOI
21 Zupan J, Muth TR, Draper J, Zambryski P (2000) The transfer of DNA from Agrobacterium tumefaciens into plants: a feast of fundamental insights. Plant J 23: 11-23   DOI   ScienceOn
22 Kumar S, Fladung M (2002) Transgene integration in aspen: Structures of integration sites and mechanism of T-DNA integration. Plant J 31: 543-551   DOI   ScienceOn
23 Hansen G, Wright MS (1999) Recent advances in the transformation of plants. Trends Plant Sci 3: 226-231
24 Southern EM (1975) Detection of specific sequences among DNA fragments. J Mol Biol 98: 503-517   DOI
25 Gelvin SB (2003) Agrobacterium-mediated plant transformation: the biology behind the "gene-jockeying" tool. Microbio and Mol Bio Rev 67: 16-37   DOI